(Circular) (Six-base) MAPSORT of: pFN21AB5846.seq Check: 1436 from: 1 to: 6160 pFN21AB5846 6160 bp With 182 enzymes: SgfI * July 6, 2012 17:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3970 278 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3503 1937 Size: 1566 4594 Acc65I G'GTAC_C Cuts at: 2888 3481 2888 Size: 593 5567 AclI AA'CG_TT Cuts at: 1563 4287 4660 1563 Size: 2724 373 3063 Fragments arranged by size: 3063 2724 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2258 3382 4223 5607 5875 1632 Size: 626 1124 841 1384 268 1917 Fragments arranged by size: 1917 1384 1124 841 626 268 AflII C'TTAA_G Cuts at: 820 1017 2132 820 Size: 197 1115 4848 Fragments arranged by size: 4848 1115 197 AflIII A'CryG_T Cuts at: 1203 5059 6036 1203 Size: 3856 977 1327 Fragments arranged by size: 3856 1327 977 AgeI A'CCGG_T Cuts at: 4991 4991 Size: 6160 AhdI GACnn_n'nnGTC Cuts at: 4889 4889 Size: 6160 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6160 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6160 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2523 2555 2523 Size: 32 6128 AlwNI CAG_nnn'CTG Cuts at: 2236 2269 2296 3903 5475 2236 Size: 33 27 1607 1572 2921 Fragments arranged by size: 2921 1607 1572 33 27 ApaLI G'TGCA_C Cuts at: 4217 5373 4217 Size: 1156 5004 ApoI r'AATT_y Cuts at: 1493 2087 2595 2858 3180 3264 3348 3432 3469 Size: 594 508 263 322 84 84 84 37 Cuts at: 3469 3695 3847 5956 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 594 508 322 263 226 152 84 84 84 37 AseI AT'TA_AT Cuts at: 160 1032 3455 4714 160 Size: 872 2423 1259 1606 Fragments arranged by size: 2423 1606 1259 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 AvaI C'yCGr_G Cuts at: 1870 1943 3485 1870 Size: 73 1542 4545 Fragments arranged by size: 4545 1542 73 BamHI G'GATC_C Cuts at: 3490 3857 3490 Size: 367 5793 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2888 3481 4936 618 Size: 325 217 627 1101 593 1455 1842 Fragments arranged by size: 1842 1455 1101 627 593 325 217 BanII G_rGCy'C Cuts at: 1391 2145 2223 2906 2990 3158 3326 3410 3479 Size: 754 78 683 84 168 168 84 69 Cuts at: 3479 1391 Size: 4072 Fragments arranged by size: 4072 754 683 168 168 84 84 78 69 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5189 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4351 4385 1903 Size: 34 2414 34 3678 Fragments arranged by size: 3678 2414 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2639 4052 5262 2639 Size: 1413 1210 3537 Fragments arranged by size: 3537 1413 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6160 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4771 136 Size: 107 122 71 631 605 3099 1525 Fragments arranged by size: 3099 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3160 6155 3160 Size: 2995 3165 BlpI GC'TnA_GC Cuts at: 3577 3577 Size: 6160 Bme1580I G_kGCm'C Cuts at: 1790 4221 5377 1790 Size: 2431 1156 2573 Fragments arranged by size: 2573 2431 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6160 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3198 3891 4849 Size: 416 53 83 399 24 1781 693 958 Cuts at: 4849 442 Size: 1753 Fragments arranged by size: 1781 1753 958 693 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6160 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2028 2396 2970 3126 3378 4820 1239 Size: 789 368 574 156 252 1442 2579 Fragments arranged by size: 2579 1442 789 574 368 252 156 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2658 4285 5150 5448 2658 Size: 1627 865 298 3370 Fragments arranged by size: 3370 1627 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2498 4823 882 Size: 617 999 2325 2219 Fragments arranged by size: 2325 2219 999 617 BsaAI yAC'GTr Cuts at: 493 1204 3951 493 Size: 711 2747 2702 Fragments arranged by size: 2747 2702 711 BsaBI GATnn'nnATC Cuts at: 2650 6154 2650 Size: 3504 2656 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3967 4349 4984 275 Size: 53 83 186 936 2434 382 635 1451 Fragments arranged by size: 2434 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4593 4991 5265 5412 5823 4593 Size: 398 274 147 411 4930 Fragments arranged by size: 4930 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2523 2553 2888 2918 1178 Size: 30 165 30 1120 30 335 30 4420 Fragments arranged by size: 4420 1120 335 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2372 2848 1186 Size: 1186 476 4498 Fragments arranged by size: 4498 1186 476 BseYI C'CCAG_C Cuts at: 2535 5363 5859 2535 Size: 2828 496 2836 Fragments arranged by size: 2836 2828 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5587 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3610 4371 5399 1540 Size: 275 186 1609 761 1028 2301 Fragments arranged by size: 2301 1609 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2145 2223 2906 3158 3326 3410 4221 4306 5377 Size: 78 683 252 168 84 811 85 1071 Cuts at: 5377 2145 Size: 2928 Fragments arranged by size: 2928 1071 811 683 252 168 85 84 78 BsmI GAATG_Cn' Cuts at: 3227 3380 3671 3764 3227 Size: 153 291 93 5623 Fragments arranged by size: 5623 291 153 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2223 2906 2990 3158 3326 3410 Size: 399 355 78 683 84 168 168 84 Cuts at: 3410 3479 4221 4306 5377 1391 Size: 69 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 683 399 355 168 168 85 84 84 78 69 BspEI T'CCGG_A Cuts at: 5823 5823 Size: 6160 BspHI T'CATG_A Cuts at: 4049 5779 4049 Size: 1730 4430 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3515 844 Size: 2671 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4047 1394 Size: 429 2224 3507 Fragments arranged by size: 3507 2224 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4655 4829 65 Size: 1183 3407 174 1396 Fragments arranged by size: 3407 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4804 4991 1700 Size: 110 2994 187 2869 Fragments arranged by size: 2994 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6160 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6160 BssSI C'ACGA_G Cuts at: 4220 5232 4220 Size: 1012 5148 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6160 BstBI TT'CG_AA Cuts at: 4964 4964 Size: 6160 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6160 BstXI CCAn_nnnn'nTGG Cuts at: 2432 2432 Size: 6160 BstYI r'GATC_y Cuts at: 1972 2247 2576 3160 3490 3857 4242 4259 5711 Size: 275 329 584 330 367 385 17 1452 Cuts at: 5711 5797 5809 6155 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 584 385 367 346 330 329 275 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2095 513 Size: 552 939 91 4578 Fragments arranged by size: 4578 939 552 91 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5421 Fragments arranged by size: 5421 683 56 BtsI GCAGTG_nn' Cuts at: 2370 3385 3676 4470 4490 2370 Size: 1015 291 794 20 4040 Fragments arranged by size: 4040 1015 794 291 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6160 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3199 3234 529 Size: 35 2635 35 3455 Fragments arranged by size: 3455 2635 35 35 DraI TTT'AAA Cuts at: 3464 3464 Size: 6160 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5167 809 Size: 507 3851 1802 Fragments arranged by size: 3851 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3607 4496 6125 Size: 54 1425 50 275 18 1777 889 1629 Cuts at: 6125 8 Size: 43 Fragments arranged by size: 1777 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3607 1537 Size: 275 1795 4090 Fragments arranged by size: 4090 1795 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3385 4090 1353 Size: 2032 705 3423 Fragments arranged by size: 3423 2032 705 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4747 5121 5267 387 Size: 1064 138 3158 374 146 1280 Fragments arranged by size: 3158 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2143 2221 2904 3156 3324 3408 2143 Size: 78 683 252 168 84 4895 Fragments arranged by size: 4895 683 252 168 84 78 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2028 2258 2396 2970 3126 3378 3382 Size: 393 396 230 138 574 156 252 4 Cuts at: 3382 4223 4820 5607 5875 1239 Size: 841 597 787 268 1524 Fragments arranged by size: 1524 841 787 597 574 396 393 268 252 230 156 138 4 EcoNI CCTnn'n_nnAGG Cuts at: 2135 2135 Size: 6160 EcoRI G'AATT_C Cuts at: 2858 3180 3264 3348 3432 3469 3847 2858 Size: 322 84 84 84 37 378 5171 Fragments arranged by size: 5171 378 322 84 84 84 37 FspI TGC'GCA Cuts at: 4666 4666 Size: 6160 HaeII r_GCGC'y Cuts at: 1678 5307 1678 Size: 3629 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2827 2859 2888 2920 3145 Size: 32 135 32 1255 32 29 32 225 Cuts at: 3145 3177 3313 3345 4801 4833 4875 4907 1373 Size: 32 136 32 1456 32 42 32 2626 Fragments arranged by size: 2626 1456 1255 225 136 135 42 32 32 32 32 32 32 32 32 29 HincII GTy'rAC Cuts at: 669 1657 1938 3422 3504 3750 669 Size: 988 281 1484 82 246 3079 Fragments arranged by size: 3079 1484 988 281 246 82 HindIII A'AGCT_T Cuts at: 748 3057 3142 748 Size: 2309 85 3766 Fragments arranged by size: 3766 2309 85 HpaI GTT'AAC Cuts at: 3750 3750 Size: 6160 KpnI G_GTAC'C Cuts at: 2892 3485 2892 Size: 593 5567 MfeI C'AATT_G Cuts at: 3759 3759 Size: 6160 MluI A'CGCG_T Cuts at: 6036 6036 Size: 6160 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5274 5458 1305 Size: 3969 184 2007 Fragments arranged by size: 3969 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4338 Fragments arranged by size: 4338 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2430 2967 4118 4477 4636 518 Size: 648 1264 537 1151 359 159 2042 Fragments arranged by size: 2042 1264 1151 648 537 359 159 MspA1I CmG'CkG Cuts at: 1606 3576 4253 5401 5646 1606 Size: 1970 677 1148 245 2120 Fragments arranged by size: 2120 1970 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2095 513 Size: 552 939 91 4578 Fragments arranged by size: 4578 939 552 91 NdeI CA'TA_TG Cuts at: 387 387 Size: 6160 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6160 NotI GC'GGCC_GC Cuts at: 3607 3607 Size: 6160 NruI TCG'CGA Cuts at: 2196 2196 Size: 6160 NspI r_CATG'y Cuts at: 1132 1229 3008 3518 5063 1132 Size: 97 1779 510 1545 2229 Fragments arranged by size: 2229 1779 1545 510 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6160 PciI A'CATG_T Cuts at: 5059 5059 Size: 6160 PflMI CCAn_nnn'nTGG Cuts at: 1108 2351 1108 Size: 1243 4917 PfoI T'CCnGG_A Cuts at: 2185 2214 2467 2185 Size: 29 253 5878 Fragments arranged by size: 5878 253 29 PmeI GTTT'AAAC Cuts at: 3464 3464 Size: 6160 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2523 2555 4229 4261 5742 5774 2523 Size: 32 1674 32 1481 32 2909 Fragments arranged by size: 2909 1674 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2746 1613 Size: 1133 5027 PsiI TTA'TAA Cuts at: 3730 3730 Size: 6160 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2400 2432 2400 Size: 32 6128 PstI C_TGCA'G Cuts at: 830 2032 2150 2695 3512 830 Size: 1202 118 545 817 3478 Fragments arranged by size: 3478 1202 817 545 118 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6160 SacI G_AGCT'C Cuts at: 2145 2223 2906 3158 3326 3410 2145 Size: 78 683 252 168 84 4895 Fragments arranged by size: 4895 683 252 168 84 78 SalI G'TCGA_C Cuts at: 1936 3502 1936 Size: 1566 4594 SapI GCTCTTCn'nnn_ Cuts at: 3385 3385 Size: 6160 SbfI CC_TGCA'GG Cuts at: 3512 3512 Size: 6160 ScaI AGT'ACT Cuts at: 2409 4408 6120 2409 Size: 1999 1712 2449 Fragments arranged by size: 2449 1999 1712 SfcI C'TryA_G Cuts at: 826 1045 2010 2028 2146 2691 3508 4643 5324 Size: 219 965 18 118 545 817 1135 681 Cuts at: 5324 5515 826 Size: 191 1471 Fragments arranged by size: 1471 1135 965 817 681 545 219 191 118 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6160 SmaI CCC'GGG Cuts at: 1872 3487 1872 Size: 1615 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2132 2673 4264 5165 5427 820 Size: 197 926 189 541 1591 901 262 1553 Fragments arranged by size: 1591 1553 926 901 541 262 197 189 SnaBI TAC'GTA Cuts at: 493 3951 493 Size: 3458 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6160 SphI G_CATG'C Cuts at: 1132 3518 1132 Size: 2386 3774 SspI AAT'ATT Cuts at: 5 52 2445 4084 5 Size: 47 2393 1639 2081 Fragments arranged by size: 2393 2081 1639 47 StuI AGG'CCT Cuts at: 1517 2324 1517 Size: 807 5353 StyI C'CwwG_G Cuts at: 513 1065 2004 2095 2256 2508 3599 513 Size: 552 939 91 161 252 1091 3074 Fragments arranged by size: 3074 1091 939 552 252 161 91 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2798 4187 4204 4357 4542 4987 325 Size: 1255 1218 1389 17 153 185 445 1498 Fragments arranged by size: 1498 1389 1255 1218 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2407 4406 6118 Size: 274 80 33 51 1443 430 1999 1712 Cuts at: 6118 96 Size: 138 Fragments arranged by size: 1999 1712 1443 430 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6120 6152 6120 Size: 32 6128 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6160 XbaI T'CTAG_A Cuts at: 3496 3496 Size: 6160 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2183 2587 2183 Size: 404 5756 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6160 XmaI C'CCGG_G Cuts at: 1870 3485 1870 Size: 1615 4545 XmnI GAAnn'nnTTC Cuts at: 2058 2265 3059 3311 4289 2058 Size: 207 794 252 978 3929 Fragments arranged by size: 3929 978 794 252 207 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3968 276 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PpuMI PspOMI PspXI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI