(Linear) (Six-base) MAPSORT of: KIBB5846.seq Check: 2430 from: 1 to: 1455 KIBB5846.orf With 182 enzymes: SgfI * July 3, 2012 16:08 .. Acc65I G'GTAC_C Cuts at: 0 883 1455 Size: 883 572 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 253 1377 1455 Size: 253 1124 78 Fragments arranged by size: 1124 253 78 AflII C'TTAA_G Cuts at: 0 127 1455 Size: 127 1328 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 518 550 1455 Size: 518 32 905 Fragments arranged by size: 905 518 32 AlwNI CAG_nnn'CTG Cuts at: 0 231 264 291 1455 Size: 231 33 27 1164 Fragments arranged by size: 1164 231 33 27 ApoI r'AATT_y Cuts at: 0 82 590 853 1175 1259 1343 1427 1455 Size: 82 508 263 322 84 84 84 28 Fragments arranged by size: 508 322 263 84 84 84 82 28 AseI AT'TA_AT Cuts at: 0 1450 1455 Size: 1450 5 BanI G'GyrC_C Cuts at: 0 883 1455 Size: 883 572 BanII G_rGCy'C Cuts at: 0 140 218 901 985 1153 1321 1405 1455 Size: 140 78 683 84 168 168 84 50 Fragments arranged by size: 683 168 168 140 84 84 78 50 BciVI GTATCCnnnnn_n' Cuts at: 0 634 1455 Size: 634 821 BglII A'GATC_T Cuts at: 0 1155 1455 Size: 1155 300 BmrI ACTGGGnnnn_n' Cuts at: 0 1193 1455 Size: 1193 262 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 23 391 965 1121 1373 1455 Size: 23 368 574 156 252 82 Fragments arranged by size: 574 368 252 156 82 23 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 653 1455 Size: 653 802 BsaI GGTCTCn'nnnn_ Cuts at: 0 493 1455 Size: 493 962 BsaBI GATnn'nnATC Cuts at: 0 645 1455 Size: 645 810 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 518 548 883 913 1455 Size: 518 30 335 30 542 Fragments arranged by size: 542 518 335 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 367 843 1455 Size: 367 476 612 Fragments arranged by size: 612 476 367 BseYI C'CCAG_C Cuts at: 0 530 1455 Size: 530 925 BsiHKAI G_wGCw'C Cuts at: 0 140 218 901 1153 1321 1405 1455 Size: 140 78 683 252 168 84 50 Fragments arranged by size: 683 252 168 140 84 78 50 BsmI GAATG_Cn' Cuts at: 0 1222 1375 1455 Size: 1222 153 80 Fragments arranged by size: 1222 153 80 Bsp1286I G_dGCh'C Cuts at: 0 140 218 901 985 1153 1321 1405 1455 Size: 140 78 683 84 168 168 84 50 Fragments arranged by size: 683 168 168 140 84 84 78 50 BstXI CCAn_nnnn'nTGG Cuts at: 0 427 1455 Size: 427 1028 BstYI r'GATC_y Cuts at: 0 242 571 1155 1455 Size: 242 329 584 300 Fragments arranged by size: 584 329 300 242 BtgI C'CryG_G Cuts at: 0 90 1455 Size: 90 1365 BtsI GCAGTG_nn' Cuts at: 0 365 1380 1455 Size: 365 1015 75 Fragments arranged by size: 1015 365 75 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1194 1229 1455 Size: 1194 35 226 Fragments arranged by size: 1194 226 35 EarI CTCTTCn'nnn_ Cuts at: 0 1380 1455 Size: 1380 75 EcoICRI GAG'CTC Cuts at: 0 138 216 899 1151 1319 1403 1455 Size: 138 78 683 252 168 84 52 Fragments arranged by size: 683 252 168 138 84 78 52 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 23 253 391 965 1121 1373 1377 1455 Size: 23 230 138 574 156 252 4 78 Fragments arranged by size: 574 252 230 156 138 78 23 4 EcoNI CCTnn'n_nnAGG Cuts at: 0 130 1455 Size: 130 1325 EcoRI G'AATT_C Cuts at: 0 853 1175 1259 1343 1427 1455 Size: 853 322 84 84 84 28 Fragments arranged by size: 853 322 84 84 84 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 822 854 883 915 1140 1172 1308 1340 Size: 822 32 29 32 225 32 136 32 Cuts at: 1340 1455 Size: 115 Fragments arranged by size: 822 225 136 115 32 32 32 32 29 HincII GTy'rAC Cuts at: 0 1417 1455 Size: 1417 38 HindIII A'AGCT_T Cuts at: 0 1052 1137 1455 Size: 1052 85 318 Fragments arranged by size: 1052 318 85 KpnI G_GTAC'C Cuts at: 0 887 1455 Size: 887 568 MslI CAynn'nnrTG Cuts at: 0 425 962 1455 Size: 425 537 493 Fragments arranged by size: 537 493 425 NcoI C'CATG_G Cuts at: 0 90 1455 Size: 90 1365 NruI TCG'CGA Cuts at: 0 191 1455 Size: 191 1264 NspI r_CATG'y Cuts at: 0 1003 1455 Size: 1003 452 PflMI CCAn_nnn'nTGG Cuts at: 0 346 1455 Size: 346 1109 PfoI T'CCnGG_A Cuts at: 0 180 209 462 1455 Size: 180 29 253 993 Fragments arranged by size: 993 253 180 29 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 518 550 1455 Size: 518 32 905 Fragments arranged by size: 905 518 32 PshAI GACnn'nnGTC Cuts at: 0 741 1455 Size: 741 714 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 395 427 1455 Size: 395 32 1028 Fragments arranged by size: 1028 395 32 PstI C_TGCA'G Cuts at: 0 27 145 690 1455 Size: 27 118 545 765 Fragments arranged by size: 765 545 118 27 SacI G_AGCT'C Cuts at: 0 140 218 901 1153 1321 1405 1455 Size: 140 78 683 252 168 84 50 Fragments arranged by size: 683 252 168 140 84 78 50 SapI GCTCTTCn'nnn_ Cuts at: 0 1380 1455 Size: 1380 75 ScaI AGT'ACT Cuts at: 0 404 1455 Size: 404 1051 SfcI C'TryA_G Cuts at: 0 5 23 141 686 1455 Size: 5 18 118 545 769 Fragments arranged by size: 769 545 118 18 5 SmlI C'TyrA_G Cuts at: 0 127 668 1455 Size: 127 541 787 Fragments arranged by size: 787 541 127 SspI AAT'ATT Cuts at: 0 440 1455 Size: 440 1015 StuI AGG'CCT Cuts at: 0 319 1455 Size: 319 1136 StyI C'CwwG_G Cuts at: 0 90 251 503 1455 Size: 90 161 252 952 Fragments arranged by size: 952 252 161 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 793 1455 Size: 793 662 TatI w'GTAC_w Cuts at: 0 402 1455 Size: 402 1053 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 178 582 1455 Size: 178 404 873 Fragments arranged by size: 873 404 178 XmnI GAAnn'nnTTC Cuts at: 0 53 260 1054 1306 1455 Size: 53 207 794 252 149 Fragments arranged by size: 794 252 207 149 53 Enzymes that do cut: Acc65I AcuI AflII AloI AlwNI ApoI AseI BanI BanII BciVI BglII BmrI BpmI BpuEI BsaI BsaBI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BstXI BstYI BtgI BtsI CspCI EarI EcoICRI Eco57MI EcoNI EcoRI Hin4I HincII HindIII KpnI MslI NcoI NruI NspI PflMI PfoI PpiI PshAI PsrI PstI SacI SapI ScaI SfcI SmlI SspI StuI StyI TaqII TatI XcmI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflIII AgeI AhdI AleI AlfI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI Bpu10I BsaAI BsaHI BsaWI BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EaeI EagI EciI EcoO109I EcoRV FalI FseI FspI FspAI HaeII HpaI KasI MfeI MluI MmeI MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NsiI PacI PasI PciI PmeI PmlI PpuMI PsiI PspOMI PspXI PvuI PvuII RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TstI Tth111I XbaI XhoI XmaI ZraI