################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AciI 1004 CCGC CCGC(-3/-1) AcsI 1063 AAATTT [GA]^AATT[CT] AflII 490 CTTAAG C^TTAAG AlwI 522 GGATC GGATC(4/5) ApoI 1063 AAATTT [GA]^AATT[CT] Asp700I 331 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 207 GGACC G^G[AT]CC BcnI 807 CCGGG CC^[GC]GG BfaI 67 CTAG C^TAG BfrI 490 CTTAAG C^TTAAG BspMI 776 ACCTGC ACCTGC(4/8) BsrDI 840 CATTGC GCAATG(2/0) DraI 901 TTTAAA TTT^AAA DraIII 500 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 61 CCATGG C^C[GA][CT]GG EaeI 344 TGGCCG [CT]^GGCC[GA] EarI 826 CTCTTC CTCTTC(1/4) EcoNI 899 CCTTTAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HapII 807 CCGG C^CGG HindIII 960 AAGCTT A^AGCTT HpaII 807 CCGG C^CGG HphI 790 TCACC GGTGA(8/7) Ksp632I 826 CTCTTC CTCTTC(1/4) MaeI 67 CTAG C^TAG MspA1I 434 CAGCTG C[AC]G^C[GT]G MspI 807 CCGG C^CGG NciI 807 CCGGG CC^[GC]GG NcoI 61 CCATGG C^CATGG NdeI 252 CATATG CA^TATG PflMI 499 CCACTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 921 GAGTC GAGTC(4/5) PvuII 434 CAGCTG CAG^CTG SfcI 1031 CTATAG C^T[GA][CT]AG Van91I 499 CCACTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 331 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AflIII 147 ACATGT A^C[GA][CT]GT 718 ACATGT BbsI 335 GTCTTC GAAGAC(2/6) 923 GTCTTC BbvI 433 GCAGC GCAGC(8/12) 436 GCTGC BpuAI 335 GTCTTC GAAGAC(2/6) 923 GTCTTC BspLU11I 147 ACATGT A^CATGT 718 ACATGT BsrI 426 CCAGT ACTGG(1/-1) 684 CCAGT BstF5I 440 CATCC GGATG(2/0) 639 CATCC Cfr13I 207 GGACC G^G[ACGT]CC 294 GGCCC DpnII 55 GATC ^GATC 523 GATC EcoT14I 61 CCATGG C^C[AT][AT]GG 317 CCTTGG Fnu4HI 433 GCAGC GC^[ACGT]GC 436 GCTGC FokI 440 CATCC GGATG(9/13) 639 CATCC HincII 568 GTTGAC GT[CT]^[GA]AC 698 GTCAAC HindII 568 GTTGAC GT[CT]^[GA]AC 698 GTCAAC ItaI 433 GCAGC GC^[ACGT]GC 436 GCTGC MboI 55 GATC ^GATC 523 GATC MslI 168 CACCCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 603 CATCAGTGTG MwoI 408 GCACCAGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 580 GCAAATGAGGC NdeII 55 GATC ^GATC 523 GATC NlaIV 322 GGTTCC GG[ACGT]^[ACGT]CC 724 GGCTCC NspI 147 ACATGT [GA]CATG^[CT] 718 ACATGT Sau3AI 55 GATC ^GATC 523 GATC Sau96I 207 GGACC G^G[ACGT]CC 294 GGCCC SfaNI 495 GATGC GCATC(5/9) 439 GCATC SmlI 490 CTTAAG C^T[CT][GA]AG 553 CTCAAG StyI 61 CCATGG C^C[AT][AT]GG 317 CCTTGG TseI 433 GCAGC G^C[AT]GC 436 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AfaI AluI BsaJI BsiYI BslI BsmFI BssKI BstNI DdeI EcoRII HaeIII HinfI MaeIII MboII MnlI MseI MvaI NlaIII RsaI ScrFI TfiI Tru9I Tsp45I Tsp509I TspRI