(Circular) (Six-base) MAPSORT of: pFN21AE1206.seq Check: 5345 from: 1 to: 5083 pFN21AE1206 5083 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2219 2219 Size: 5083 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2893 278 Size: 53 83 186 936 1357 2468 Fragments arranged by size: 2468 1357 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2426 1937 Size: 489 4594 Acc65I G'GTAC_C Cuts at: 2404 2404 Size: 5083 AclI AA'CG_TT Cuts at: 1563 3210 3583 1563 Size: 1647 373 3063 Fragments arranged by size: 3063 1647 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2162 3146 4530 4798 1632 Size: 530 984 1384 268 1917 Fragments arranged by size: 1917 1384 984 530 268 AfeI AGC'GCT Cuts at: 2264 2377 2264 Size: 113 4970 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4886 AflIII A'CryG_T Cuts at: 1203 3982 4959 1203 Size: 2779 977 1327 Fragments arranged by size: 2779 1327 977 AgeI A'CCGG_T Cuts at: 3914 3914 Size: 5083 AhdI GACnn_n'nnGTC Cuts at: 3812 3812 Size: 5083 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5083 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5083 AlwNI CAG_nnn'CTG Cuts at: 2826 4398 2826 Size: 1572 3511 ApaLI G'TGCA_C Cuts at: 3140 4296 3140 Size: 1156 3927 ApoI r'AATT_y Cuts at: 1493 2194 2392 2618 2770 4879 1493 Size: 701 198 226 152 2109 1697 Fragments arranged by size: 2109 1697 701 226 198 152 AseI AT'TA_AT Cuts at: 160 1032 3637 160 Size: 872 2605 1606 Fragments arranged by size: 2605 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5083 AvaI C'yCGr_G Cuts at: 1870 1943 2408 1870 Size: 73 465 4545 Fragments arranged by size: 4545 465 73 BamHI G'GATC_C Cuts at: 2413 2780 2413 Size: 367 4716 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2404 3859 618 Size: 325 217 627 617 1455 1842 Fragments arranged by size: 1842 1455 627 617 325 217 BanII G_rGCy'C Cuts at: 1391 2048 2402 1391 Size: 657 354 4072 Fragments arranged by size: 4072 657 354 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4112 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3274 3308 1903 Size: 34 50 34 1253 34 3678 Fragments arranged by size: 3678 1253 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2975 4185 2975 Size: 1210 3873 BclI T'GATC_A Cuts at: 1548 1548 Size: 5083 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3694 136 Size: 107 122 71 631 605 2022 1525 Fragments arranged by size: 2022 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5078 5078 Size: 5083 BlpI GC'TnA_GC Cuts at: 2500 2500 Size: 5083 Bme1580I G_kGCm'C Cuts at: 1790 3144 4300 1790 Size: 1354 1156 2573 Fragments arranged by size: 2573 1354 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5083 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2814 3772 442 Size: 416 53 83 399 24 1397 958 1753 Fragments arranged by size: 1753 1397 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5083 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2263 3743 1239 Size: 1024 1480 2579 Fragments arranged by size: 2579 1480 1024 Bpu10I CC'TnA_GC Cuts at: 2379 2379 Size: 5083 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3208 4073 4371 3208 Size: 865 298 3920 Fragments arranged by size: 3920 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3746 882 Size: 617 2247 2219 Fragments arranged by size: 2247 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2874 493 Size: 711 1670 2702 Fragments arranged by size: 2702 1670 711 BsaBI GATnn'nnATC Cuts at: 5077 5077 Size: 5083 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2890 3272 3907 275 Size: 53 83 186 936 1357 382 635 1451 Fragments arranged by size: 1451 1357 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3516 3914 4188 4335 4746 3516 Size: 398 274 147 411 3853 Fragments arranged by size: 3853 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4858 Fragments arranged by size: 4858 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5083 BseYI C'CCAG_C Cuts at: 4286 4782 4286 Size: 496 4587 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4510 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2298 2533 3294 4322 1540 Size: 275 186 297 235 761 1028 2301 Fragments arranged by size: 2301 1028 761 297 275 235 186 BsiHKAI G_wGCw'C Cuts at: 3144 3229 4300 3144 Size: 85 1071 3927 Fragments arranged by size: 3927 1071 85 BsmI GAATG_Cn' Cuts at: 2594 2687 2594 Size: 93 4990 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2402 3144 3229 4300 1391 Size: 399 258 354 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 354 258 85 BspEI T'CCGG_A Cuts at: 4746 4746 Size: 5083 BspHI T'CATG_A Cuts at: 2190 2972 4702 2190 Size: 782 1730 2571 Fragments arranged by size: 2571 1730 782 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2137 2219 2438 844 Size: 1293 82 219 3489 Fragments arranged by size: 3489 1293 219 82 BsrBI CCG'CTC Cuts at: 1394 1823 2970 1394 Size: 429 1147 3507 Fragments arranged by size: 3507 1147 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3578 3752 65 Size: 1183 2330 174 1396 Fragments arranged by size: 2330 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2162 3727 3914 1700 Size: 110 352 1565 187 2869 Fragments arranged by size: 2869 1565 352 187 110 BsrGI T'GTAC_A Cuts at: 96 2130 96 Size: 2034 3049 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5083 BssSI C'ACGA_G Cuts at: 3143 4155 3143 Size: 1012 4071 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5083 BstBI TT'CG_AA Cuts at: 3887 3887 Size: 5083 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5083 BstXI CCAn_nnnn'nTGG Cuts at: 2182 2182 Size: 5083 BstYI r'GATC_y Cuts at: 1972 2286 2413 2780 3165 3182 4634 4720 4732 Size: 314 127 367 385 17 1452 86 12 Cuts at: 4732 5078 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 314 127 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2181 513 Size: 552 1116 3415 Fragments arranged by size: 3415 1116 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2209 487 Size: 683 56 983 3361 Fragments arranged by size: 3361 983 683 56 BtsI GCAGTG_nn' Cuts at: 2599 3393 3413 2599 Size: 794 20 4269 Fragments arranged by size: 4269 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5083 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5048 DraI TTT'AAA Cuts at: 2387 2387 Size: 5083 DraIII CAC_nnn'GTG Cuts at: 2338 2338 Size: 5083 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4090 809 Size: 507 2774 1802 Fragments arranged by size: 2774 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2295 2325 2530 Size: 54 1425 50 275 18 465 30 205 Cuts at: 2530 3419 5048 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 465 275 205 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2295 2530 1537 Size: 275 483 235 4090 Fragments arranged by size: 4090 483 275 235 EarI CTCTTCn'nnn_ Cuts at: 1353 3013 1353 Size: 1660 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3670 4044 4190 387 Size: 1064 138 2081 374 146 1280 Fragments arranged by size: 2081 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2162 2263 3146 3743 4530 4798 1239 Size: 393 530 101 883 597 787 268 1524 Fragments arranged by size: 1524 883 787 597 530 393 268 101 EcoRI G'AATT_C Cuts at: 2194 2392 2770 2194 Size: 198 378 4507 Fragments arranged by size: 4507 378 198 FspI TGC'GCA Cuts at: 3589 3589 Size: 5083 HaeII r_GCGC'y Cuts at: 1678 2266 2379 4230 1678 Size: 588 113 1851 2531 Fragments arranged by size: 2531 1851 588 113 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3724 3756 3798 3830 1373 Size: 32 135 32 2152 32 42 32 2626 Fragments arranged by size: 2626 2152 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2427 2673 669 Size: 988 281 489 246 3079 Fragments arranged by size: 3079 988 489 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5083 HpaI GTT'AAC Cuts at: 2673 2673 Size: 5083 KpnI G_GTAC'C Cuts at: 2408 2408 Size: 5083 MfeI C'AATT_G Cuts at: 2682 2682 Size: 5083 MluI A'CGCG_T Cuts at: 4959 4959 Size: 5083 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4197 4381 1305 Size: 2892 184 2007 Fragments arranged by size: 2892 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2327 10 Size: 54 1425 343 495 2766 Fragments arranged by size: 2766 1425 495 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2126 3041 3400 3559 518 Size: 648 960 915 359 159 2042 Fragments arranged by size: 2042 960 915 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2499 3176 4324 4569 1606 Size: 893 677 1148 245 2120 Fragments arranged by size: 2120 1148 893 677 245 NcoI C'CATG_G Cuts at: 513 1065 2181 513 Size: 552 1116 3415 Fragments arranged by size: 3415 1116 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5083 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5083 NotI GC'GGCC_GC Cuts at: 2530 2530 Size: 5083 NspI r_CATG'y Cuts at: 1132 1229 2441 3986 1132 Size: 97 1212 1545 2229 Fragments arranged by size: 2229 1545 1212 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5083 PciI A'CATG_T Cuts at: 3982 3982 Size: 5083 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5083 PmeI GTTT'AAAC Cuts at: 2387 2387 Size: 5083 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3152 3184 4665 4697 3152 Size: 32 1481 32 3538 Fragments arranged by size: 3538 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5083 PsiI TTA'TAA Cuts at: 2653 2653 Size: 5083 PstI C_TGCA'G Cuts at: 830 2435 830 Size: 1605 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5083 SalI G'TCGA_C Cuts at: 1936 2425 1936 Size: 489 4594 SbfI CC_TGCA'GG Cuts at: 2435 2435 Size: 5083 ScaI AGT'ACT Cuts at: 3331 5043 3331 Size: 1712 3371 SfcI C'TryA_G Cuts at: 826 1045 2431 3566 4247 4438 826 Size: 219 1386 1135 681 191 1471 Fragments arranged by size: 1471 1386 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5083 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5083 SmaI CCC'GGG Cuts at: 1872 2410 1872 Size: 538 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3187 4088 4350 820 Size: 197 926 1244 901 262 1553 Fragments arranged by size: 1553 1244 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2874 493 Size: 2381 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5083 SphI G_CATG'C Cuts at: 1132 2441 1132 Size: 1309 3774 SspI AAT'ATT Cuts at: 5 52 3007 5 Size: 47 2955 2081 Fragments arranged by size: 2955 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5083 StyI C'CwwG_G Cuts at: 513 1065 2175 2181 2522 513 Size: 552 1110 6 341 3074 Fragments arranged by size: 3074 1110 552 341 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3110 3127 3280 3465 3910 325 Size: 1255 1530 17 153 185 445 1498 Fragments arranged by size: 1530 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2130 2367 3329 Size: 274 80 33 51 1443 153 237 962 Cuts at: 3329 5041 96 Size: 1712 138 Fragments arranged by size: 1712 1443 962 274 237 153 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2324 2356 5043 5075 2324 Size: 32 2687 32 2332 Fragments arranged by size: 2687 2332 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5083 XbaI T'CTAG_A Cuts at: 2419 2419 Size: 5083 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5083 XmaI C'CCGG_G Cuts at: 1870 2408 1870 Size: 538 4545 XmnI GAAnn'nnTTC Cuts at: 3212 3212 Size: 5083 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2891 276 Size: 53 83 186 936 1357 2468 Fragments arranged by size: 2468 1357 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI