################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI AspEI AspI AviII AvrII BamHI BbeI BbrPI BciVI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT14I FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MflI MluI MroI MseI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 509 GGTACC G^GTACC Alw44I 68 GTGCAC G^TGCAC AlwNI 8 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 100 GGGCCC GGGCC^C ApaLI 68 GTGCAC G^TGCAC Asp700I 916 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 509 GGTACC G^GTACC AvaI 163 CCCGAG C^[CT]CG[GA]G BalI 119 TGGCCA TGG^CCA BbsI 469 GAAGAC GAAGAC(2/6) BcgI 165 CGAGAAAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 620 TGATCA T^GATCA BcnI 29 CCCGG CC^[GC]GG BlpI 979 GCTGAGC GC^T[ACGT]AGC Bpu1102I 979 GCTGAGC GC^T[ACGT]AGC BpuAI 469 GAAGAC GAAGAC(2/6) BsaWI 200 TCCGGT [AT]^CCGG[AT] BsmFI 536 GGGAC GGGAC(10/14) BsoBI 163 CCCGAG C^[CT]CG[GA]G BspLU11I 647 ACATGT A^CATGT BspMI 389 GCAGGT ACCTGC(4/8) BsrDI 783 CATTGC GCAATG(2/0) BstEII 606 GGTCACC G^GT[ACGT]ACC BstPI 606 GGTCACC G^GT[ACGT]ACC CelII 979 GCTGAGC GC^T[ACGT]AGC CfoI 556 GCGC GCG^C DraIII 525 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 598 CTCTTC CTCTTC(1/4) EcoNI 564 CCTGAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 606 GGTCACC G^GT[ACGT]ACC EcoT22I 792 ATGCAT ATGCA^T FauI 217 CCCGC CCCGC(4/6) FbaI 620 TGATCA T^GATCA HhaI 556 GCGC GCG^C HinP1I 556 GCGC G^CGC KpnI 509 GGTACC GGTAC^C Ksp632I 598 CTCTTC CTCTTC(1/4) MluNI 119 TGGCCA TGG^CCA MscI 119 TGGCCA TGG^CCA NciI 29 CCCGG CC^[GC]GG NsiI 792 ATGCAT ATGCA^T PvuII 8 CAGCTG CAG^CTG SacI 982 GAGCTC GAGCT^C XbaI 499 TCTAGA T^CTAGA XmnI 916 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AfaI 273 GTAC GT^AC 510 GTAC AflIII 647 ACATGT A^C[GA][CT]GT 900 ACGTGT AluI 9 AGCT AG^CT 983 AGCT AlwI 109 GATCC GGATC(4/5) 803 GGATC AspHI 68 GTGCAC G[AT]GC[AT]^C 982 GAGCTC AvaII 392 GGTCC G^G[AT]CC 537 GGACC BanI 361 GGCACC G^G[CT][GA]CC 509 GGTACC BanII 100 GGGCCC G[GA]GC[CT]^C 982 GAGCTC BbvCI 57 CCTCAGC CCTCAGC(-5/-2) 175 GCTGAGG BfaI 433 CTAG C^TAG 500 CTAG BpmI 487 CTGGAG CTGGAG(16/14) 889 CTGGAG BsiHKAI 68 GTGCAC G[AT]GC[AT]^C 982 GAGCTC BstF5I 408 CATCC GGATG(2/0) 480 CATCC BstNI 327 CCTGG CC^[AT]GG 351 CCAGG Cac8I 33 GCCAGC GC[ACGT]^[ACGT]GC 558 GCCTGC DsaI 122 CCACGG C^C[GA][CT]GG 611 CCACGG EcoRII 327 CCTGG ^CC[AT]GG 351 CCAGG FokI 408 CATCC GGATG(9/13) 480 CATCC HphI 160 TCACC GGTGA(8/7) 608 TCACC MaeI 433 CTAG C^TAG 500 CTAG MaeII 142 ACGT A^CGT 900 ACGT MspA1I 8 CAGCTG C[AC]G^C[GT]G 369 CCGCTG MvaI 327 CCTGG CC^[AT]GG 351 CCAGG MwoI 311 GCAGGGGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 362 GCACCTACCGC NspI 647 ACATGT [GA]CATG^[CT] 975 ACATGC PpuMI 391 AGGTCCT [GA]G^G[AT]CC[CT] 536 GGGACCC RsaI 273 GTAC GT^AC 510 GTAC Tsp45I 159 GTCAC ^GT[GC]AC 607 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI BbvI BmyI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI Bsp1286I BsrI BssKI Cfr13I DdeI DpnII DraII EaeI EcoO109I Fnu4HI HaeIII HapII HinfI HpaII ItaI MaeIII MboI MboII MnlI MslI MspI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TfiI TseI Tsp509I TspRI