(Circular) (Six-base) MAPSORT of: pFN21AE6606.seq Check: 3086 from: 1 to: 5707 pFN21AE6606 5707 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3517 278 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3050 1937 Size: 1113 4594 Acc65I G'GTAC_C Cuts at: 2514 3028 2514 Size: 514 5193 AclI AA'CG_TT Cuts at: 1563 3006 3834 4207 1563 Size: 1443 828 373 3063 Fragments arranged by size: 3063 1443 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3770 5154 5422 1632 Size: 2138 1384 268 1917 Fragments arranged by size: 2138 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5510 AflIII A'CryG_T Cuts at: 1203 2652 2905 4606 5583 1203 Size: 1449 253 1701 977 1327 Fragments arranged by size: 1701 1449 1327 977 253 AgeI A'CCGG_T Cuts at: 4538 4538 Size: 5707 AhdI GACnn_n'nnGTC Cuts at: 4436 4436 Size: 5707 AleI CACnn'nnGTG Cuts at: 1166 2297 2621 1166 Size: 1131 324 4252 Fragments arranged by size: 4252 1131 324 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5707 AlwNI CAG_nnn'CTG Cuts at: 2018 3450 5022 2018 Size: 1432 1572 2703 Fragments arranged by size: 2703 1572 1432 ApaI G_GGCC'C Cuts at: 2109 2109 Size: 5707 ApaLI G'TGCA_C Cuts at: 2073 3764 4920 2073 Size: 1691 1156 2860 Fragments arranged by size: 2860 1691 1156 ApoI r'AATT_y Cuts at: 1493 3016 3242 3394 5503 1493 Size: 1523 226 152 2109 1697 Fragments arranged by size: 2109 1697 1523 226 152 AseI AT'TA_AT Cuts at: 160 1032 4261 160 Size: 872 3229 1606 Fragments arranged by size: 3229 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 AvaI C'yCGr_G Cuts at: 1870 1943 2168 3032 1870 Size: 73 225 864 4545 Fragments arranged by size: 4545 864 225 73 BamHI G'GATC_C Cuts at: 3037 3404 3037 Size: 367 5340 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2366 2514 3028 4483 618 Size: 325 217 627 579 148 514 1455 1842 Fragments arranged by size: 1842 1455 627 579 514 325 217 148 BanII G_rGCy'C Cuts at: 1391 2109 2991 3026 1391 Size: 718 882 35 4072 Fragments arranged by size: 4072 882 718 35 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2481 928 Size: 971 582 4154 Fragments arranged by size: 4154 971 582 BbvCI CC'TCA_GC Cuts at: 2063 2181 2063 Size: 118 5589 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2159 2193 3898 3932 1903 Size: 34 222 34 1705 34 3678 Fragments arranged by size: 3678 1705 222 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3599 4809 3599 Size: 1210 4497 BclI T'GATC_A Cuts at: 1548 2625 1548 Size: 1077 4630 BfrBI ATG'CAT Cuts at: 2799 2799 Size: 5707 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4318 136 Size: 107 122 71 631 605 2646 1525 Fragments arranged by size: 2646 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5702 5702 Size: 5707 BlpI GC'TnA_GC Cuts at: 2985 3124 2985 Size: 139 5568 Bme1580I G_kGCm'C Cuts at: 1790 2077 2109 3768 4924 1790 Size: 287 32 1659 1156 2573 Fragments arranged by size: 2573 1659 1156 287 32 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5707 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2083 2724 3438 Size: 416 53 83 399 24 666 641 714 Cuts at: 3438 4396 442 Size: 958 1753 Fragments arranged by size: 1753 958 714 666 641 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5707 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2513 2915 4367 1239 Size: 1274 402 1452 2579 Fragments arranged by size: 2579 1452 1274 402 Bpu10I CC'TnA_GC Cuts at: 2063 2181 2063 Size: 118 5589 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3832 4697 4995 3832 Size: 865 298 4544 Fragments arranged by size: 4544 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2086 2214 2584 4370 882 Size: 617 587 128 370 1786 2219 Fragments arranged by size: 2219 1786 617 587 370 128 BsaAI yAC'GTr Cuts at: 493 1204 3498 493 Size: 711 2294 2702 Fragments arranged by size: 2702 2294 711 BsaBI GATnn'nnATC Cuts at: 5701 5701 Size: 5707 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3514 3896 4531 275 Size: 53 83 186 936 1981 382 635 1451 Fragments arranged by size: 1981 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2205 4140 4538 4812 4959 5370 2205 Size: 1935 398 274 147 411 2542 Fragments arranged by size: 2542 1935 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2183 2213 1178 Size: 30 165 30 780 30 4672 Fragments arranged by size: 4672 780 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 2042 2045 2048 2051 2276 2288 1186 Size: 853 3 3 3 3 225 12 4605 Fragments arranged by size: 4605 853 225 12 3 3 3 3 BseYI C'CCAG_C Cuts at: 4910 5406 4910 Size: 496 5211 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2009 2402 2766 1163 Size: 573 273 393 364 4104 Fragments arranged by size: 4104 573 393 364 273 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3157 3918 4946 1540 Size: 275 186 1156 761 1028 2301 Fragments arranged by size: 2301 1156 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2077 2991 3768 3853 4924 2077 Size: 914 777 85 1071 2860 Fragments arranged by size: 2860 1071 914 777 85 BsmI GAATG_Cn' Cuts at: 3218 3311 3218 Size: 93 5614 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2077 2109 2991 3026 3768 3853 4924 Size: 399 287 32 882 35 742 85 1071 Cuts at: 4924 1391 Size: 2174 Fragments arranged by size: 2174 1071 882 742 399 287 85 35 32 BspEI T'CCGG_A Cuts at: 5370 5370 Size: 5707 BspHI T'CATG_A Cuts at: 3596 5326 3596 Size: 1730 3977 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2385 3062 844 Size: 1541 677 3489 Fragments arranged by size: 3489 1541 677 BsrBI CCG'CTC Cuts at: 1394 1823 3594 1394 Size: 429 1771 3507 Fragments arranged by size: 3507 1771 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2787 4202 4376 65 Size: 1183 1539 1415 174 1396 Fragments arranged by size: 1539 1415 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4351 4538 1700 Size: 110 2541 187 2869 Fragments arranged by size: 2869 2541 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5707 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5707 BssSI C'ACGA_G Cuts at: 3767 4779 3767 Size: 1012 4695 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5707 BstBI TT'CG_AA Cuts at: 4511 4511 Size: 5707 BstEII G'GTnAC_C Cuts at: 1184 2611 1184 Size: 1427 4280 BstYI r'GATC_y Cuts at: 1972 3037 3404 3789 3806 5258 5344 5356 5702 Size: 1065 367 385 17 1452 86 12 346 Cuts at: 5702 1972 Size: 1977 Fragments arranged by size: 1977 1452 1065 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2127 2616 513 Size: 552 939 123 489 3604 Fragments arranged by size: 3604 939 552 489 123 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2974 487 Size: 683 56 1748 3220 Fragments arranged by size: 3220 1748 683 56 BtsI GCAGTG_nn' Cuts at: 2232 2754 3223 4017 4037 2232 Size: 522 469 794 20 3902 Fragments arranged by size: 3902 794 522 469 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5707 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5672 DraI TTT'AAA Cuts at: 3011 3011 Size: 5707 DraIII CAC_nnn'GTG Cuts at: 2535 2535 Size: 5707 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4714 809 Size: 507 3398 1802 Fragments arranged by size: 3398 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2036 2124 2683 Size: 54 1425 50 275 18 206 88 559 Cuts at: 2683 3154 4043 5672 8 Size: 471 889 1629 43 Fragments arranged by size: 1629 1425 889 559 471 275 206 88 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3154 1537 Size: 275 1342 4090 Fragments arranged by size: 4090 1342 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2609 3637 1353 Size: 1256 1028 3423 Fragments arranged by size: 3423 1256 1028 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4294 4668 4814 387 Size: 1064 138 2705 374 146 1280 Fragments arranged by size: 2705 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2989 2989 Size: 5707 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2513 2915 3770 4367 5154 5422 1239 Size: 393 881 402 855 597 787 268 1524 Fragments arranged by size: 1524 881 855 787 597 402 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2573 2573 Size: 5707 EcoO109I rG'GnC_Cy Cuts at: 2105 2397 2542 2105 Size: 292 145 5270 Fragments arranged by size: 5270 292 145 EcoRI G'AATT_C Cuts at: 3016 3394 3016 Size: 378 5329 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2960 2960 Size: 5707 FspI TGC'GCA Cuts at: 4213 4213 Size: 5707 HaeII r_GCGC'y Cuts at: 1678 4854 1678 Size: 3176 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2486 2518 4348 4380 4422 Size: 32 135 32 914 32 1830 32 42 Cuts at: 4422 4454 1373 Size: 32 2626 Fragments arranged by size: 2626 1830 914 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3051 3297 669 Size: 988 281 1113 246 3079 Fragments arranged by size: 3079 1113 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5707 HpaI GTT'AAC Cuts at: 3297 3297 Size: 5707 KpnI G_GTAC'C Cuts at: 2518 3032 2518 Size: 514 5193 MfeI C'AATT_G Cuts at: 3306 3306 Size: 5707 MluI A'CGCG_T Cuts at: 5583 5583 Size: 5707 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2113 4821 5005 1305 Size: 808 2708 184 2007 Fragments arranged by size: 2708 2007 808 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2126 10 Size: 54 1425 343 294 3591 Fragments arranged by size: 3591 1425 343 294 54 MslI CAynn'nnrTG Cuts at: 518 1166 2192 2297 2459 2621 3665 4024 4183 Size: 648 1026 105 162 162 1044 359 159 Cuts at: 4183 518 Size: 2042 Fragments arranged by size: 2042 1044 1026 648 359 162 162 159 105 MspA1I CmG'CkG Cuts at: 1606 2015 2376 3123 3800 4948 5193 1606 Size: 409 361 747 677 1148 245 2120 Fragments arranged by size: 2120 1148 747 677 409 361 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4216 Fragments arranged by size: 4216 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5707 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5707 NotI GC'GGCC_GC Cuts at: 3154 3154 Size: 5707 NsiI A_TGCA'T Cuts at: 2801 2801 Size: 5707 NspI r_CATG'y Cuts at: 1132 1229 2656 2984 3065 4610 1132 Size: 97 1427 328 81 1545 2229 Fragments arranged by size: 2229 1545 1427 328 97 81 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5707 PciI A'CATG_T Cuts at: 2652 4606 2652 Size: 1954 3753 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5707 PmeI GTTT'AAAC Cuts at: 3011 3011 Size: 5707 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3776 3808 5289 5321 3776 Size: 32 1481 32 4162 Fragments arranged by size: 4162 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2397 2542 2397 Size: 145 5562 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5707 PsiI TTA'TAA Cuts at: 3277 3277 Size: 5707 PspOMI G'GGCC_C Cuts at: 2105 2105 Size: 5707 PstI C_TGCA'G Cuts at: 830 3059 830 Size: 2229 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5707 PvuII CAG'CTG Cuts at: 2015 2015 Size: 5707 SacI G_AGCT'C Cuts at: 2991 2991 Size: 5707 SalI G'TCGA_C Cuts at: 1936 3049 1936 Size: 1113 4594 SanDI GG'GwC_CC Cuts at: 2542 2542 Size: 5707 SbfI CC_TGCA'GG Cuts at: 3059 3059 Size: 5707 ScaI AGT'ACT Cuts at: 3955 5667 3955 Size: 1712 3995 SfcI C'TryA_G Cuts at: 826 1045 3055 4190 4871 5062 826 Size: 219 2010 1135 681 191 1471 Fragments arranged by size: 2010 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5707 SmaI CCC'GGG Cuts at: 1872 3034 1872 Size: 1162 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3811 4712 4974 820 Size: 197 926 1868 901 262 1553 Fragments arranged by size: 1868 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3498 493 Size: 3005 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5707 SphI G_CATG'C Cuts at: 1132 3065 1132 Size: 1933 3774 SspI AAT'ATT Cuts at: 5 52 3631 5 Size: 47 3579 2081 Fragments arranged by size: 3579 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5707 StyI C'CwwG_G Cuts at: 513 1065 2004 3146 513 Size: 552 939 1142 3074 Fragments arranged by size: 3074 1142 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3734 3751 3904 4089 4534 325 Size: 1255 2154 17 153 185 445 1498 Fragments arranged by size: 2154 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3953 5665 96 Size: 274 80 33 51 1443 1976 1712 138 Fragments arranged by size: 1976 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2183 2215 5667 5699 2183 Size: 32 3452 32 2191 Fragments arranged by size: 3452 2191 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5707 XbaI T'CTAG_A Cuts at: 2504 3043 2504 Size: 539 5168 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5707 XmaI C'CCGG_G Cuts at: 1870 3032 1870 Size: 1162 4545 XmnI GAAnn'nnTTC Cuts at: 2925 3836 2925 Size: 911 4796 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3515 276 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI