Restriction map for
pFN21AE9605
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII AccI AclI AcsI AflIII
AhdI Alw44I AlwI AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspHI AspI AvaI
AviII BamHI BanII BbrPI BbsI
BcgI BciVI BclI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1286I
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstNI BstPI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DpnII DraI DraII DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRII EcoRV EcoT22I
FbaI FseI FspI HgaI HincII
HindII HpaI Ksp632I KspI MaeII
MamI MboI MfeI MflI MluI
MslI MunI MvaI NaeI NcoI
NdeI NdeII NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tsp45I
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 295 GGTACC G^GTACC
AccII 183 CGCG CG^CG
AccIII 109 TCCGGA T^CCGGA
AcyI 430 GGCGCC G[GA]^CG[CT]C
AflII 187 CTTAAG C^TTAAG
AgeI 298 ACCGGT A^CCGGT
AlwNI 119 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 195 AGCGCT AGC^GCT
Asp718I 295 GGTACC G^GTACC
AvrII 385 CCTAGG C^CTAGG
BalI 512 TGGCCA TGG^CCA
BbeI 430 GGCGCC GGCGC^C
BbvCI 541 CCTCAGC CCTCAGC(-5/-2)
BcnI 341 CCGGG CC^[GC]GG
BfaI 386 CTAG C^TAG
BfrI 187 CTTAAG C^TTAAG
BglI 361 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 385 CCTAGG C^CTAGG
BsaHI 430 GGCGCC G[GA]^CG[CT]C
BseAI 109 TCCGGA T^CCGGA
BsmAI 7 GAGAC GTCTC(1/5)
BsmFI 499 GGGAC GGGAC(10/14)
BspEI 109 TCCGGA T^CCGGA
BsrFI 298 ACCGGT [GA]^CCGG[CT]
BsrI 118 CCAGT ACTGG(1/-1)
BssKI 341 CCGGG ^CC[ACGT]GG
BstAPI 487 GCAACCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 107 CATCC GGATG(2/0)
BstUI 183 CGCG CG^CG
Cfr10I 298 ACCGGT [GA]^CCGG[CT]
Eco47III 195 AGCGCT AGC^GCT
FauI 40 CCCGC CCCGC(4/6)
FokI 107 CATCC GGATG(9/13)
Hin1I 430 GGCGCC G[GA]^CG[CT]C
HindIII 244 AAGCTT A^AGCTT
KasI 430 GGCGCC G^GCGCC
KpnI 295 GGTACC GGTAC^C
MaeI 386 CTAG C^TAG
MaeIII 628 GTTAC ^GT[ACGT]AC
MluNI 512 TGGCCA TGG^CCA
MroI 109 TCCGGA T^CCGGA
MscI 512 TGGCCA TGG^CCA
MseI 188 TTAA T^TAA
MvnI 183 CGCG CG^CG
NarI 430 GGCGCC GG^CGCC
NciI 341 CCGGG CC^[GC]GG
PinAI 298 ACCGGT A^CCGGT
ScrFI 341 CCGGG CC^[ACGT]GG
SfoI 430 GGCGCC GGC^GCC
Tru9I 188 TTAA T^TAA
TspRI 143 CAGTG CA[GC]TG[ACGT][ACGT]^
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# TWO CUT ENZYMES ( 15 enzymes) #
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AfaI 275 GTAC GT^AC
296 GTAC
AvaII 112 GGACC G^G[AT]CC
500 GGACC
BanI 295 GGTACC G^G[CT][GA]CC
430 GGCGCC
BsaWI 109 TCCGGA [AT]^CCGG[AT]
298 ACCGGT
BsgI 35 CTGCAC GTGCAG(16/14)
283 GTGCAG
Cfr13I 112 GGACC G^G[ACGT]CC
500 GGACC
HaeII 195 AGCGCT [GA]GCGC^[CT]
430 GGCGCC
HinfI 462 GACTC G^A[ACGT]TC
564 GAGTC
MnlI 252 CCTC CCTC(7/6)
541 CCTC
MspA1I 165 CAGCGG C[AC]G^C[GT]G
491 CCGCTG
PleI 462 GACTC GAGTC(4/5)
564 GAGTC
RsaI 275 GTAC GT^AC
296 GTAC
Sau96I 112 GGACC G^G[ACGT]CC
500 GGACC
SfaNI 106 GCATC GCATC(5/9)
452 GCATC
SmlI 187 CTTAAG C^T[CT][GA]AG
253 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BstXI Cac8I CfoI DdeI
EaeI EcoT14I Fnu4HI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MboII MspI MwoI NlaIV StyI
TseI