(Linear) (Six-base) MAPSORT of: KIEE9605.seq  Check: 4726  from: 1  to: 663



KIEE9605.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 18:32  ..

Acc65I G'GTAC_C

Cuts at:      0     295     663 
   Size:        295     368
 
AfeI AGC'GCT

Cuts at:      0     197     663 
   Size:        197     466
 
AflII C'TTAA_G

Cuts at:      0     187     663 
   Size:        187     476
 
AgeI A'CCGG_T

Cuts at:      0     298     663 
   Size:        298     365
 
AlwNI CAG_nnn'CTG

Cuts at:      0     124     663 
   Size:        124     539
 
AvrII C'CTAG_G

Cuts at:      0     385     663 
   Size:        385     278
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     222     255     266     299     663 
   Size:        222      33      11      33     364

  Fragments arranged by size: 

                 364     222      33      33      11
 
BanI G'GyrC_C

Cuts at:      0     295     430     663 
   Size:        295     135     233

  Fragments arranged by size: 

                 295     233     135
 
BbeI G_GCGC'C

Cuts at:      0     434     663 
   Size:        434     229
 
BbvCI CC'TCA_GC

Cuts at:      0     542     663 
   Size:        542     121
 
BglI GCCn_nnn'nGGC

Cuts at:      0     367     663 
   Size:        367     296
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     112     663 
   Size:        112     551
 
Bpu10I CC'TnA_GC

Cuts at:      0     542     572     587     663 
   Size:        542      30      15      76

  Fragments arranged by size: 

                 542      76      30      15
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     663 
   Size:        238     425
 
BsaHI Gr'CG_yC

Cuts at:      0     431     663 
   Size:        431     232
 
BsaWI w'CCGG_w

Cuts at:      0     109     298     663 
   Size:        109     189     365

  Fragments arranged by size: 

                 365     189     109
 
BseYI C'CCAG_C

Cuts at:      0      91     397     496     663 
   Size:         91     306      99     167

  Fragments arranged by size: 

                 306     167      99      91
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      20     304     663 
   Size:         20     284     359

  Fragments arranged by size: 

                 359     284      20
 
BspEI T'CCGG_A

Cuts at:      0     109     663 
   Size:        109     554
 
BsrFI r'CCGG_y

Cuts at:      0     298     663 
   Size:        298     365
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     493     663 
   Size:        493     170
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     144     423     510     663 
   Size:        144     279      87     153

  Fragments arranged by size: 

                 279     153     144      87
 
BtsI GCAGTG_nn'

Cuts at:      0     149     663 
   Size:        149     514
 
EaeI y'GGCC_r

Cuts at:      0     179     512     599     663 
   Size:        179     333      87      64

  Fragments arranged by size: 

                 333     179      87      64
 
HaeII r_GCGC'y

Cuts at:      0     199     434     663 
   Size:        199     235     229

  Fragments arranged by size: 

                 235     229     199
 
HindIII A'AGCT_T

Cuts at:      0     244     663 
   Size:        244     419
 
KasI G'GCGC_C

Cuts at:      0     430     663 
   Size:        430     233
 
KpnI G_GTAC'C

Cuts at:      0     299     663 
   Size:        299     364
 
MscI TGG'CCA

Cuts at:      0     514     663 
   Size:        514     149
 
MspA1I CmG'CkG

Cuts at:      0     167     493     663 
   Size:        167     326     170

  Fragments arranged by size: 

                 326     170     167
 
NarI GG'CG_CC

Cuts at:      0     431     663 
   Size:        431     232
 
SfoI GGC'GCC

Cuts at:      0     432     663 
   Size:        432     231
 
SmlI C'TyrA_G

Cuts at:      0     187     253     663 
   Size:        187      66     410

  Fragments arranged by size: 

                 410     187      66
 
StyI C'CwwG_G

Cuts at:      0     137     356     385     593     663 
   Size:        137     219      29     208      70

  Fragments arranged by size: 

                 219     208     137      70      29
 

 Enzymes that do cut:

   Acc65I     AfeI    AflII     AgeI    AlwNI    AvrII     BaeI     BanI
     BbeI    BbvCI     BglI     BmrI   Bpu10I    BpuEI    BsaHI    BsaWI
    BseYI     BsgI    BspEI    BsrFI   BstAPI    BstXI     BtsI     EaeI
    HaeII  HindIII     KasI     KpnI     MscI   MspA1I     NarI     SfoI
     SmlI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI   AflIII     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
     AvaI    BamHI    BanII     BbsI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI     BsaI
    BsaAI    BsaBI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII     HpaI     MfeI     MluI     MmeI
     MslI     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI