################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I CelII ClaI CpoI DpnII DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MunI NcoI NdeI NdeII NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacII SalI Sau3AI SbfI ScaI SexAI SfiI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AcyI 549 GGCGCC G[GA]^CG[CT]C AocI 352 CCTAAGG CC^T[ACGT]AGG Aor51HI 192 AGCGCT AGC^GCT ApaI 109 GGGCCC GGGCC^C AspHI 124 GAGCTC G[AT]GC[AT]^C BbeI 549 GGCGCC GGCGC^C BcnI 114 CCCGG CC^[GC]GG BpmI 494 CTGGAG CTGGAG(16/14) BsaHI 549 GGCGCC G[GA]^CG[CT]C BsaI 247 GGTCTC GGTCTC(1/5) BsgI 280 GTGCAG GTGCAG(16/14) BsiHKAI 124 GAGCTC G[AT]GC[AT]^C BspMI 82 GCAGGT ACCTGC(4/8) Bsu36I 352 CCTAAGG CC^T[ACGT]AGG DdeI 353 CTAAG C^T[ACGT]AG DraII 109 GGGCCCC [GA]G^G[ACGT]CC[CT] EarI 444 GAAGAG CTCTTC(1/4) Eco47III 192 AGCGCT AGC^GCT Eco81I 352 CCTAAGG CC^T[ACGT]AGG EcoO109I 109 GGGCCCC [GA]G^G[ACGT]CC[CT] Hin1I 549 GGCGCC G[GA]^CG[CT]C HinfI 51 GACTC G^A[ACGT]TC HphI 261 GGTGA GGTGA(8/7) KasI 549 GGCGCC G^GCGCC Ksp632I 444 GAAGAG CTCTTC(1/4) MaeII 215 ACGT A^CGT MseI 581 TTAA T^TAA MslI 273 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 480 GCCGGC GCC^GGC NarI 549 GGCGCC GG^CGCC NciI 114 CCCGG CC^[GC]GG NgoMIV 480 GCCGGC G^CCGGC PleI 51 GACTC GAGTC(4/5) PvuII 488 CAGCTG CAG^CTG SacI 124 GAGCTC GAGCT^C SapI 444 GAAGAGC GCTCTTC(1/4) SfaNI 236 GCATC GCATC(5/9) SfoI 549 GGCGCC GGC^GCC SgrAI 294 CACCGGCG C[GA]^CCGG[CT]G Tru9I 581 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AlwNI 200 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG 488 CAGCTGCTG BbsI 48 GAAGAC GAAGAC(2/6) 456 GAAGAC BpuAI 48 GAAGAC GAAGAC(2/6) 456 GAAGAC BsmAI 7 GAGAC GTCTC(1/5) 248 GTCTC BsrBI 179 CCGCTC CCGCTC(-3/-3) 398 GAGCGG BsrFI 295 ACCGGC [GA]^CCGG[CT] 480 GCCGGC BstNI 258 CCTGG CC^[AT]GG 276 CCTGG Cfr10I 295 ACCGGC [GA]^CCGG[CT] 480 GCCGGC EcoRII 258 CCTGG ^CC[AT]GG 276 CCTGG FauI 25 CCCGC CCCGC(4/6) 71 CCCGC HgaI 103 GCGTC GACGC(5/10) 301 GCGTC MnlI 149 CCTC CCTC(7/6) 335 CCTC MvaI 258 CCTGG CC^[AT]GG 276 CCTGG PstI 80 CTGCAG CTGCA^G 485 CTGCAG SfcI 80 CTGCAG C^T[GA][CT]AG 485 CTGCAG SmlI 184 CTCAAG C^T[CT][GA]AG 250 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AccII AciI AluI BanI BanII BbvI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstUI Cac8I CfoI Cfr13I EaeI EcoT14I Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MboII MspA1I MspI MvnI MwoI NlaIV Sau96I ScrFI StyI TseI