(Circular) (Six-base) MAPSORT of: pFN21AE9604.seq Check: 6384 from: 1 to: 5362 pFN21AE9604 5362 bp With 182 enzymes: SgfI * March 28, 2012 12:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2078 2078 Size: 5362 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3172 278 Size: 53 83 186 936 1636 2468 Fragments arranged by size: 2468 1636 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2705 1937 Size: 768 4594 Acc65I G'GTAC_C Cuts at: 2683 2683 Size: 5362 AclI AA'CG_TT Cuts at: 1563 3489 3862 1563 Size: 1926 373 3063 Fragments arranged by size: 3063 1926 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3425 4809 5077 1632 Size: 1793 1384 268 1917 Fragments arranged by size: 1917 1793 1384 268 AfeI AGC'GCT Cuts at: 2199 2199 Size: 5362 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5165 AflIII A'CryG_T Cuts at: 1203 4261 5238 1203 Size: 3058 977 1327 Fragments arranged by size: 3058 1327 977 AgeI A'CCGG_T Cuts at: 4193 4193 Size: 5362 AhdI GACnn_n'nnGTC Cuts at: 4091 4091 Size: 5362 AleI CACnn'nnGTG Cuts at: 1166 2282 1166 Size: 1116 4246 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2300 1184 Size: 1116 4246 AlwNI CAG_nnn'CTG Cuts at: 2210 2498 3105 4677 2210 Size: 288 607 1572 2895 Fragments arranged by size: 2895 1572 607 288 ApaI G_GGCC'C Cuts at: 2118 2118 Size: 5362 ApaLI G'TGCA_C Cuts at: 3419 4575 3419 Size: 1156 4206 ApoI r'AATT_y Cuts at: 1493 2671 2897 3049 5158 1493 Size: 1178 226 152 2109 1697 Fragments arranged by size: 2109 1697 1178 226 152 AseI AT'TA_AT Cuts at: 160 1032 3916 160 Size: 872 2884 1606 Fragments arranged by size: 2884 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5362 AvaI C'yCGr_G Cuts at: 1870 1943 2687 1870 Size: 73 744 4545 Fragments arranged by size: 4545 744 73 BamHI G'GATC_C Cuts at: 2692 3059 2692 Size: 367 4995 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2276 2297 2554 2683 4138 Size: 325 217 627 489 21 257 129 1455 Cuts at: 4138 618 Size: 1842 Fragments arranged by size: 1842 1455 627 489 325 257 217 129 21 BanII G_rGCy'C Cuts at: 1391 2118 2133 2567 2681 1391 Size: 727 15 434 114 4072 Fragments arranged by size: 4072 727 434 114 15 BbeI G_GCGC'C Cuts at: 2558 2558 Size: 5362 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2060 2468 928 Size: 971 161 408 3822 Fragments arranged by size: 3822 971 408 161 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3553 3587 1903 Size: 34 1616 34 3678 Fragments arranged by size: 3678 1616 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3254 4464 3254 Size: 1210 4152 BclI T'GATC_A Cuts at: 1548 1548 Size: 5362 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3973 136 Size: 107 122 71 631 605 2301 1525 Fragments arranged by size: 2301 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5357 5357 Size: 5362 BlpI GC'TnA_GC Cuts at: 2779 2779 Size: 5362 Bme1580I G_kGCm'C Cuts at: 1790 2118 2279 2300 3423 4579 1790 Size: 328 161 21 1123 1156 2573 Fragments arranged by size: 2573 1156 1123 328 161 21 BmgBI CAC'GTC Cuts at: 1318 2221 1318 Size: 903 4459 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3093 4051 442 Size: 416 53 83 399 24 1676 958 1753 Fragments arranged by size: 1753 1676 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5362 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2520 4022 1239 Size: 1281 1502 2579 Fragments arranged by size: 2579 1502 1281 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2174 2240 3487 4352 4650 2174 Size: 66 1247 865 298 2886 Fragments arranged by size: 2886 1247 865 298 66 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2258 4025 882 Size: 617 759 1767 2219 Fragments arranged by size: 2219 1767 759 617 BsaAI yAC'GTr Cuts at: 493 1204 3153 493 Size: 711 1949 2702 Fragments arranged by size: 2702 1949 711 BsaBI GATnn'nnATC Cuts at: 5356 5356 Size: 5362 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2555 3169 3551 4186 Size: 53 83 186 936 1022 614 382 635 Cuts at: 4186 275 Size: 1451 Fragments arranged by size: 1451 1022 936 635 614 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3795 4193 4467 4614 5025 3795 Size: 398 274 147 411 4132 Fragments arranged by size: 4132 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5137 Fragments arranged by size: 5137 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5362 BseYI C'CCAG_C Cuts at: 2248 2566 4565 5061 2248 Size: 318 1999 496 2549 Fragments arranged by size: 2549 1999 496 318 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2306 1163 Size: 573 570 4219 Fragments arranged by size: 4219 573 570 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2812 3573 4601 1540 Size: 275 186 811 761 1028 2301 Fragments arranged by size: 2301 1028 811 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2133 3423 3508 4579 2133 Size: 1290 85 1071 2916 Fragments arranged by size: 2916 1290 1071 85 BsmI GAATG_Cn' Cuts at: 2873 2966 2873 Size: 93 5269 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2133 2279 2300 2567 2681 3423 Size: 399 328 15 146 21 267 114 742 Cuts at: 3423 3508 4579 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 328 267 146 114 85 21 15 BspEI T'CCGG_A Cuts at: 5025 5025 Size: 5362 BspHI T'CATG_A Cuts at: 3251 4981 3251 Size: 1730 3632 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2078 2717 844 Size: 1234 639 3489 Fragments arranged by size: 3489 1234 639 BsrBI CCG'CTC Cuts at: 1394 1823 2186 2405 3249 1394 Size: 429 363 219 844 3507 Fragments arranged by size: 3507 844 429 363 219 BsrDI GCAATG_nn' Cuts at: 65 1248 3857 4031 65 Size: 1183 2609 174 1396 Fragments arranged by size: 2609 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2300 2485 4006 4193 1700 Size: 110 490 185 1521 187 2869 Fragments arranged by size: 2869 1521 490 187 185 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5362 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5362 BssSI C'ACGA_G Cuts at: 3422 4434 3422 Size: 1012 4350 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5362 BstBI TT'CG_AA Cuts at: 4166 4166 Size: 5362 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5362 BstYI r'GATC_y Cuts at: 1972 2692 3059 3444 3461 4913 4999 5011 5357 Size: 720 367 385 17 1452 86 12 346 Cuts at: 5357 1972 Size: 1977 Fragments arranged by size: 1977 1452 720 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2358 2358 Size: 5362 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4810 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4623 Fragments arranged by size: 4623 683 56 BtsI GCAGTG_nn' Cuts at: 2878 3672 3692 2878 Size: 794 20 4548 Fragments arranged by size: 4548 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5362 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5327 DraI TTT'AAA Cuts at: 2666 2666 Size: 5362 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4369 809 Size: 507 3053 1802 Fragments arranged by size: 3053 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2094 2181 2379 Size: 54 1425 50 275 18 264 87 198 Cuts at: 2379 2436 2809 3698 5327 8 Size: 57 373 889 1629 43 Fragments arranged by size: 1629 1425 889 373 275 264 198 87 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2809 1537 Size: 275 997 4090 Fragments arranged by size: 4090 997 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2444 3292 1353 Size: 1091 848 3423 Fragments arranged by size: 3423 1091 848 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3949 4323 4469 387 Size: 1064 138 2360 374 146 1280 Fragments arranged by size: 2360 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2131 2131 Size: 5362 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2520 3425 4022 4809 5077 1239 Size: 393 888 905 597 787 268 1524 Fragments arranged by size: 1524 905 888 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2115 2115 Size: 5362 EcoRI G'AATT_C Cuts at: 2671 3049 2671 Size: 378 4984 FspI TGC'GCA Cuts at: 3868 3868 Size: 5362 HaeII r_GCGC'y Cuts at: 1678 2201 2457 2558 4509 1678 Size: 523 256 101 1951 2531 Fragments arranged by size: 2531 1951 523 256 101 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4003 4035 4077 4109 1373 Size: 32 135 32 2431 32 42 32 2626 Fragments arranged by size: 2626 2431 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2706 2952 669 Size: 988 281 768 246 3079 Fragments arranged by size: 3079 988 768 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5362 HpaI GTT'AAC Cuts at: 2952 2952 Size: 5362 KasI G'GCGC_C Cuts at: 2554 2554 Size: 5362 KpnI G_GTAC'C Cuts at: 2687 2687 Size: 5362 MfeI C'AATT_G Cuts at: 2961 2961 Size: 5362 MluI A'CGCG_T Cuts at: 5238 5238 Size: 5362 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4476 4660 1305 Size: 3171 184 2007 Fragments arranged by size: 3171 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3540 Fragments arranged by size: 3540 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2282 3320 3679 3838 518 Size: 648 1116 1038 359 159 2042 Fragments arranged by size: 2042 1116 1038 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2033 2169 2210 2495 2778 3455 4603 4848 Size: 427 136 41 285 283 677 1148 245 Cuts at: 4848 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 427 285 283 245 136 41 NaeI GCC'GGC Cuts at: 2487 2487 Size: 5362 NarI GG'CG_CC Cuts at: 2555 2555 Size: 5362 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4810 NdeI CA'TA_TG Cuts at: 387 387 Size: 5362 NgoMIV G'CCGG_C Cuts at: 2485 2485 Size: 5362 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5362 NotI GC'GGCC_GC Cuts at: 2809 2809 Size: 5362 NspI r_CATG'y Cuts at: 1132 1229 2720 4265 1132 Size: 97 1491 1545 2229 Fragments arranged by size: 2229 1545 1491 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5362 PciI A'CATG_T Cuts at: 4261 4261 Size: 5362 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5362 PmeI GTTT'AAAC Cuts at: 2666 2666 Size: 5362 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3431 3463 4944 4976 3431 Size: 32 1481 32 3817 Fragments arranged by size: 3817 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5362 PsiI TTA'TAA Cuts at: 2932 2932 Size: 5362 PspOMI G'GGCC_C Cuts at: 2114 2114 Size: 5362 PstI C_TGCA'G Cuts at: 830 2089 2494 2714 830 Size: 1259 405 220 3478 Fragments arranged by size: 3478 1259 405 220 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5362 PvuII CAG'CTG Cuts at: 2495 2495 Size: 5362 SacI G_AGCT'C Cuts at: 2133 2133 Size: 5362 SalI G'TCGA_C Cuts at: 1936 2704 1936 Size: 768 4594 SapI GCTCTTCn'nnn_ Cuts at: 2444 2444 Size: 5362 SbfI CC_TGCA'GG Cuts at: 2714 2714 Size: 5362 ScaI AGT'ACT Cuts at: 3610 5322 3610 Size: 1712 3650 SfcI C'TryA_G Cuts at: 826 1045 2085 2490 2710 3845 4526 4717 826 Size: 219 1040 405 220 1135 681 191 1471 Fragments arranged by size: 1471 1135 1040 681 405 220 219 191 SfoI GGC'GCC Cuts at: 2556 2556 Size: 5362 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5362 SgrAI Cr'CCGG_yG Cuts at: 1700 2300 1700 Size: 600 4762 SmaI CCC'GGG Cuts at: 1872 2689 1872 Size: 817 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2189 2255 3466 4367 4629 820 Size: 197 926 246 66 1211 901 262 1553 Fragments arranged by size: 1553 1211 926 901 262 246 197 66 SnaBI TAC'GTA Cuts at: 493 3153 493 Size: 2660 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5362 SphI G_CATG'C Cuts at: 1132 2720 1132 Size: 1588 3774 SspI AAT'ATT Cuts at: 5 52 3286 5 Size: 47 3234 2081 Fragments arranged by size: 3234 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5362 StyI C'CwwG_G Cuts at: 513 1065 2157 2292 2382 2592 2622 2801 513 Size: 552 1092 135 90 210 30 179 3074 Fragments arranged by size: 3074 1092 552 210 179 135 90 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3389 3406 3559 3744 4189 325 Size: 1255 1809 17 153 185 445 1498 Fragments arranged by size: 1809 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3608 5320 96 Size: 274 80 33 51 1443 1631 1712 138 Fragments arranged by size: 1712 1631 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5322 5354 5322 Size: 32 5330 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5362 XbaI T'CTAG_A Cuts at: 2698 2698 Size: 5362 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5362 XmaI C'CCGG_G Cuts at: 1870 2687 1870 Size: 817 4545 XmnI GAAnn'nnTTC Cuts at: 3491 3491 Size: 5362 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3170 276 Size: 53 83 186 936 1636 2468 Fragments arranged by size: 2468 1636 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI