################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BciVI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FseI FspI HgaI Hin1I HpaI KasI KpnI MamI MfeI MflI MluI MluNI MroI MscI MunI NarI NciI NdeI NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SalI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SwaI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AflIII 287 ACATGT A^C[GA][CT]GT ApaI 3 GGGCCC GGGCC^C AspHI 853 GAGCAC G[AT]GC[AT]^C BbsI 382 GAAGAC GAAGAC(2/6) BcgI 513 GCACAAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 183 TGATCA T^GATCA BfaI 913 CTAG C^TAG BglI 13 GCCAGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 382 GAAGAC GAAGAC(2/6) BsiHKAI 853 GAGCAC G[AT]GC[AT]^C BspHI 186 TCATGA T^CATGA BspLU11I 287 ACATGT A^CATGT BspMI 354 GCAGGT ACCTGC(4/8) BsrFI 18 GCCGGC [GA]^CCGG[CT] BstAPI 573 GCAGCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 751 TTCGAA TT^CGAA BstXI 491 CCACCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 18 GCCGGC [GA]^CCGG[CT] EcoRI 439 GAATTC G^AATTC FauI 6 CCCGC CCCGC(4/6) FbaI 183 TGATCA T^GATCA HapII 19 CCGG C^CGG HincII 298 GTTGAC GT[CT]^[GA]AC HindII 298 GTTGAC GT[CT]^[GA]AC HindIII 143 AAGCTT A^AGCTT HpaII 19 CCGG C^CGG KspI 50 CCGCGG CCGC^GG MaeI 913 CTAG C^TAG MseI 985 TTAA T^TAA MslI 702 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 19 CCGG C^CGG NaeI 18 GCCGGC GCC^GGC NgoMIV 18 GCCGGC G^CCGGC NspI 287 ACATGT [GA]CATG^[CT] NspV 751 TTCGAA TT^CGAA RcaI 186 TCATGA T^CATGA SacII 50 CCGCGG CCGC^GG SapI 826 GAAGAGC GCTCTTC(1/4) SfuI 751 TTCGAA TT^CGAA SspI 981 AATATT AAT^ATT StuI 861 AGGCCT AGG^CCT TaqI 752 TCGA T^CGA TfiI 519 GATTC G^A[AT]TC Tru9I 985 TTAA T^TAA TthHB8I 752 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccII 8 CGCG CG^CG 51 CGCG AlwI 282 GGATC GGATC(4/5) 867 GGATC BanI 106 GGTGCC G^G[CT][GA]CC 907 GGCACC BsaAI 154 TACGTG [CT]AC^GT[GA] 840 TACGTG BseRI 28 CTCCTC GAGGAG(10/8) 1007 GAGGAG BsgI 88 CTGCAC GTGCAG(16/14) 563 CTGCAC BstUI 8 CGCG CG^CG 51 CGCG CfoI 22 GCGC GCG^C 66 GCGC HaeII 21 GGCGCT [GA]GCGC^[CT] 65 GGCGCT HhaI 22 GCGC GCG^C 66 GCGC HinP1I 22 GCGC G^CGC 66 GCGC MvnI 8 CGCG CG^CG 51 CGCG NcoI 217 CCATGG C^CATGG 1013 CCATGG PflMI 479 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 707 CCATGAAGTGG PpuMI 119 AGGACCT [GA]G^G[AT]CC[CT] 784 GGGACCT PshAI 48 GACCGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 121 GACCTTGGTC SbfI 57 CCTGCAGG CCTGCA^GG 351 CCTGCAGG SfaNI 36 GATGC GCATC(5/9) 739 GATGC Sse8387I 57 CCTGCAGG CCTGCA^GG 351 CCTGCAGG Van91I 479 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 707 CCATGAAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AcsI AfaI AluI AlwNI ApoI AvaII BanII BbvI BmyI BpmI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfcI StyI TseI Tsp45I Tsp509I TspRI