Restriction map for
pFN21AE5431
(Circular) (Six-base) MAPSORT of: pFN21AE5431.seq Check: 9197 from: 1 to: 5749
pFN21AE5431 5749 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3559 278
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3092 1937
Size: 1155 4594
Acc65I G'GTAC_C
Cuts at: 3070 3070
Size: 5749
AclI AA'CG_TT
Cuts at: 1563 3876 4249 1563
Size: 2313 373 3063
Fragments arranged by size:
3063 2313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2643 2744 3812 5196 5464 1632
Size: 1011 101 1068 1384 268 1917
Fragments arranged by size:
1917 1384 1068 1011 268 101
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5552
AflIII A'CryG_T
Cuts at: 1203 2292 4648 5625 1203
Size: 1089 2356 977 1327
Fragments arranged by size:
2356 1327 1089 977
AgeI A'CCGG_T
Cuts at: 4580 4580
Size: 5749
AhdI GACnn_n'nnGTC
Cuts at: 4478 4478
Size: 5749
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5749
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5749
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2279 2311 2279
Size: 32 5717
AlwNI CAG_nnn'CTG
Cuts at: 2275 2490 2587 3492 5064 2275
Size: 215 97 905 1572 2960
Fragments arranged by size:
2960 1572 905 215 97
ApaI G_GGCC'C
Cuts at: 2012 2012
Size: 5749
ApaLI G'TGCA_C
Cuts at: 3806 4962 3806
Size: 1156 4593
ApoI r'AATT_y
Cuts at: 1493 2238 2444 2973 3058 3284 3436 5545 1493
Size: 745 206 529 85 226 152 2109 1697
Fragments arranged by size:
2109 1697 745 529 226 206 152 85
AseI AT'TA_AT
Cuts at: 160 1032 4303 160
Size: 872 3271 1606
Fragments arranged by size:
3271 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
AvaI C'yCGr_G
Cuts at: 1870 1943 3074 1870
Size: 73 1131 4545
Fragments arranged by size:
4545 1131 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2380 2413 2380
Size: 33 5716
BamHI G'GATC_C
Cuts at: 3079 3446 3079
Size: 367 5382
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2111 2912 3070 4525 618
Size: 325 217 627 324 801 158 1455 1842
Fragments arranged by size:
1842 1455 801 627 325 324 217 158
BanII G_rGCy'C
Cuts at: 1391 2012 2214 2901 3068 1391
Size: 621 202 687 167 4072
Fragments arranged by size:
4072 687 621 202 167
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2394 928
Size: 971 495 4283
Fragments arranged by size:
4283 971 495
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2507 2541 3940 3974 1903
Size: 34 570 34 1399 34 3678
Fragments arranged by size:
3678 1399 570 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3641 4851 3641
Size: 1210 4539
BclI T'GATC_A
Cuts at: 1548 2188 1548
Size: 640 5109
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2024 4360 136
Size: 107 122 71 631 605 352 2336 1525
Fragments arranged by size:
2336 1525 631 605 352 122 107 71
BglII A'GATC_T
Cuts at: 5744 5744
Size: 5749
BlpI GC'TnA_GC
Cuts at: 3166 3166
Size: 5749
Bme1580I G_kGCm'C
Cuts at: 1790 2012 3027 3810 4966 1790
Size: 222 1015 783 1156 2573
Fragments arranged by size:
2573 1156 1015 783 222
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5749
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3480 4438 442
Size: 416 53 83 399 24 2063 958 1753
Fragments arranged by size:
2063 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2525 2573 2609 4409 1239
Size: 1286 48 36 1800 2579
Fragments arranged by size:
2579 1800 1286 48 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3874 4739 5037 3874
Size: 865 298 4586
Fragments arranged by size:
4586 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4412 882
Size: 617 2913 2219
Fragments arranged by size:
2913 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2161 2847 3540 493
Size: 711 957 686 693 2702
Fragments arranged by size:
2702 957 711 693 686
BsaBI GATnn'nnATC
Cuts at: 5743 5743
Size: 5749
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3556 3938 4573 275
Size: 53 83 186 936 2023 382 635 1451
Fragments arranged by size:
2023 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4182 4580 4854 5001 5412 4182
Size: 398 274 147 411 4519
Fragments arranged by size:
4519 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5524
Fragments arranged by size:
5524 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 3027 1186
Size: 838 1003 3908
Fragments arranged by size:
3908 1003 838
BseYI C'CCAG_C
Cuts at: 2593 4952 5448 2593
Size: 2359 496 2894
Fragments arranged by size:
2894 2359 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2078 2553 1163
Size: 573 342 475 4359
Fragments arranged by size:
4359 573 475 342
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3199 3960 4988 1540
Size: 275 186 1198 761 1028 2301
Fragments arranged by size:
2301 1198 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2862 3810 3895 4966 2862
Size: 948 85 1071 3645
Fragments arranged by size:
3645 1071 948 85
BsmI GAATG_Cn'
Cuts at: 3260 3353 3260
Size: 93 5656
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2214 2862 2901 3027 3068 3810
Size: 399 222 202 648 39 126 41 742
Cuts at: 3810 3895 4966 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 648 399 222 202 126
85 41 39
BspEI T'CCGG_A
Cuts at: 5412 5412
Size: 5749
BspHI T'CATG_A
Cuts at: 2191 3638 5368 2191
Size: 1447 1730 2572
Fragments arranged by size:
2572 1730 1447
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2350 3104 844
Size: 1506 754 3489
Fragments arranged by size:
3489 1506 754
BsrBI CCG'CTC
Cuts at: 1394 1823 3636 1394
Size: 429 1813 3507
Fragments arranged by size:
3507 1813 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4244 4418 65
Size: 1183 2996 174 1396
Fragments arranged by size:
2996 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2023 4393 4580 1700
Size: 110 213 2370 187 2869
Fragments arranged by size:
2869 2370 213 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5749
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5749
BssSI C'ACGA_G
Cuts at: 3809 4821 3809
Size: 1012 4737
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2584 794
Size: 1790 3959
BstBI TT'CG_AA
Cuts at: 2757 4553 2757
Size: 1796 3953
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5749
BstXI CCAn_nnnn'nTGG
Cuts at: 2503 2503
Size: 5749
BstYI r'GATC_y
Cuts at: 1972 3079 3446 3831 3848 5300 5386 5398 5744
Size: 1107 367 385 17 1452 86 12 346
Cuts at: 5744 1972
Size: 1977
Fragments arranged by size:
1977 1452 1107 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2055 2222 3018 513
Size: 552 939 51 167 796 3244
Fragments arranged by size:
3244 939 796 552 167 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5010
Fragments arranged by size:
5010 683 56
BtsI GCAGTG_nn'
Cuts at: 3265 4059 4079 3265
Size: 794 20 4935
Fragments arranged by size:
4935 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5749
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5714
DraI TTT'AAA
Cuts at: 3053 3053
Size: 5749
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4756 809
Size: 507 3440 1802
Fragments arranged by size:
3440 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3196 4085 5714
Size: 54 1425 50 275 18 1366 889 1629
Cuts at: 5714 8
Size: 43
Fragments arranged by size:
1629 1425 1366 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3196 1537
Size: 275 1384 4090
Fragments arranged by size:
4090 1384 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2108 2662 2826 2990 3679 1353
Size: 755 554 164 164 689 3423
Fragments arranged by size:
3423 755 689 554 164 164
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4336 4710 4856 387
Size: 1064 138 2747 374 146 1280
Fragments arranged by size:
2747 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2525 2573 2609 2643 2744 3812 4409
Size: 393 893 48 36 34 101 1068 597
Cuts at: 4409 5196 5464 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1068 893 787 597 393 268 101
48 36 34
EcoO109I rG'GnC_Cy
Cuts at: 2125 2487 2544 2790 2125
Size: 362 57 246 5084
Fragments arranged by size:
5084 362 246 57
EcoRI G'AATT_C
Cuts at: 2444 3058 3436 2444
Size: 614 378 4757
Fragments arranged by size:
4757 614 378
FspI TGC'GCA
Cuts at: 4255 4255
Size: 5749
HaeII r_GCGC'y
Cuts at: 1678 2030 2074 4896 1678
Size: 352 44 2822 2531
Fragments arranged by size:
2822 2531 352 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2257 2289 4390 4422 4464
Size: 32 135 32 685 32 2101 32 42
Cuts at: 4464 4496 1373
Size: 32 2626
Fragments arranged by size:
2626 2101 685 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2305 3093 3339 669
Size: 988 281 367 788 246 3079
Fragments arranged by size:
3079 988 788 367 281 246
HindIII A'AGCT_T
Cuts at: 748 2148 748
Size: 1400 4349
HpaI GTT'AAC
Cuts at: 3339 3339
Size: 5749
KpnI G_GTAC'C
Cuts at: 3074 3074
Size: 5749
MfeI C'AATT_G
Cuts at: 3348 3348
Size: 5749
MluI A'CGCG_T
Cuts at: 5625 5625
Size: 5749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4863 5047 1305
Size: 3558 184 2007
Fragments arranged by size:
3558 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3927
Fragments arranged by size:
3927 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2711 3707 4066 4225 518
Size: 648 1545 996 359 159 2042
Fragments arranged by size:
2042 1545 996 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2057 2173 2263 2575 3165 3842 4990 5235
Size: 451 116 90 312 590 677 1148 245
Cuts at: 5235 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 590 451 312 245 116
90
NaeI GCC'GGC
Cuts at: 2025 2025
Size: 5749
NcoI C'CATG_G
Cuts at: 513 1065 2004 2222 3018 513
Size: 552 939 218 796 3244
Fragments arranged by size:
3244 939 796 552 218
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5749
NgoMIV G'CCGG_C
Cuts at: 2023 2023
Size: 5749
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5749
NotI GC'GGCC_GC
Cuts at: 3196 3196
Size: 5749
NspI r_CATG'y
Cuts at: 1132 1229 2296 3107 4652 1132
Size: 97 1067 811 1545 2229
Fragments arranged by size:
2229 1545 1067 811 97
PasI CC'CwG_GG
Cuts at: 1348 2484 2825 1348
Size: 1136 341 4272
Fragments arranged by size:
4272 1136 341
PciI A'CATG_T
Cuts at: 2292 4648 2292
Size: 2356 3393
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2490 2718 1108
Size: 1382 228 4139
Fragments arranged by size:
4139 1382 228
PmeI GTTT'AAAC
Cuts at: 3053 3053
Size: 5749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2989 3021 3818 3850 5331 5363 2989
Size: 32 797 32 1481 32 3375
Fragments arranged by size:
3375 1481 797 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2125 2790 2125
Size: 665 5084
PshAI GACnn'nnGTC
Cuts at: 1613 2057 2130 1613
Size: 444 73 5232
Fragments arranged by size:
5232 444 73
PsiI TTA'TAA
Cuts at: 3319 3319
Size: 5749
PspOMI G'GGCC_C
Cuts at: 2008 2008
Size: 5749
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2531 2563 2531
Size: 32 5717
PstI C_TGCA'G
Cuts at: 830 2067 2262 2361 2580 3101 830
Size: 1237 195 99 219 521 3478
Fragments arranged by size:
3478 1237 521 219 195 99
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5749
PvuII CAG'CTG
Cuts at: 2173 2263 2575 2173
Size: 90 312 5347
Fragments arranged by size:
5347 312 90
SacII CC_GC'GG
Cuts at: 2058 2058
Size: 5749
SalI G'TCGA_C
Cuts at: 1936 3091 1936
Size: 1155 4594
SapI GCTCTTCn'nnn_
Cuts at: 2826 2826
Size: 5749
SbfI CC_TGCA'GG
Cuts at: 2067 2361 3101 2067
Size: 294 740 4715
Fragments arranged by size:
4715 740 294
ScaI AGT'ACT
Cuts at: 3997 5709 3997
Size: 1712 4037
SfcI C'TryA_G
Cuts at: 826 1045 2063 2258 2357 2576 3097 4232 4913
Size: 219 1018 195 99 219 521 1135 681
Cuts at: 4913 5104 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1018 681 521 219 219 195
191 99
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5749
SmaI CCC'GGG
Cuts at: 1872 3076 1872
Size: 1204 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3853 4754 5016 820
Size: 197 926 1910 901 262 1553
Fragments arranged by size:
1910 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3540 493
Size: 3047 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5749
SphI G_CATG'C
Cuts at: 1132 3107 1132
Size: 1975 3774
SspI AAT'ATT
Cuts at: 5 52 2988 3673 5
Size: 47 2936 685 2081
Fragments arranged by size:
2936 2081 685 47
StuI AGG'CCT
Cuts at: 1517 2868 1517
Size: 1351 4398
StyI C'CwwG_G
Cuts at: 513 1065 2004 2128 2222 2748 2811 3018 3188
Size: 552 939 124 94 526 63 207 170
Cuts at: 3188 513
Size: 3074
Fragments arranged by size:
3074 939 552 526 207 170 124 94
63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2099 2877 3776 3793 3946 4131 4576
Size: 1255 519 778 899 17 153 185 445
Cuts at: 4576 325
Size: 1498
Fragments arranged by size:
1498 1255 899 778 519 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3995 5707 96
Size: 274 80 33 51 1443 2018 1712 138
Fragments arranged by size:
2018 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5709 5741 5709
Size: 32 5717
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5749
XbaI T'CTAG_A
Cuts at: 3085 3085
Size: 5749
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5749
XmaI C'CCGG_G
Cuts at: 1870 3074 1870
Size: 1204 4545
XmnI GAAnn'nnTTC
Cuts at: 3878 3878
Size: 5749
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3557 276
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PspXI RsrII SacI SanDI SexAI
SfiI SfoI SrfI SwaI XcmI