(Linear) (Six-base) MAPSORT of: KIEE5431.seq Check: 7550 from: 1 to: 1044 KIEE5431.orf With 182 enzymes: SgfI * January 29, 2014 12:00 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 638 739 1044 Size: 638 101 305 Fragments arranged by size: 638 305 101 AflIII A'CryG_T Cuts at: 0 287 1044 Size: 287 757 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 274 306 1044 Size: 274 32 738 Fragments arranged by size: 738 274 32 AlwNI CAG_nnn'CTG Cuts at: 0 270 485 582 1044 Size: 270 215 97 462 Fragments arranged by size: 462 270 215 97 ApaI G_GGCC'C Cuts at: 0 7 1044 Size: 7 1037 ApoI r'AATT_y Cuts at: 0 233 439 968 1044 Size: 233 206 529 76 Fragments arranged by size: 529 233 206 76 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 375 408 1044 Size: 375 33 636 Fragments arranged by size: 636 375 33 BanI G'GyrC_C Cuts at: 0 106 907 1044 Size: 106 801 137 Fragments arranged by size: 801 137 106 BanII G_rGCy'C Cuts at: 0 7 209 896 1044 Size: 7 202 687 148 Fragments arranged by size: 687 202 148 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 389 1044 Size: 389 655 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 502 536 1044 Size: 502 34 508 Fragments arranged by size: 508 502 34 BclI T'GATC_A Cuts at: 0 183 1044 Size: 183 861 BglI GCCn_nnn'nGGC Cuts at: 0 19 1044 Size: 19 1025 Bme1580I G_kGCm'C Cuts at: 0 7 1022 1044 Size: 7 1015 22 Fragments arranged by size: 1015 22 7 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 520 568 604 1044 Size: 520 48 36 440 Fragments arranged by size: 520 440 48 36 BsaAI yAC'GTr Cuts at: 0 156 842 1044 Size: 156 686 202 Fragments arranged by size: 686 202 156 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 19 1022 1044 Size: 19 1003 22 Fragments arranged by size: 1003 22 19 BseYI C'CCAG_C Cuts at: 0 588 1044 Size: 588 456 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 73 548 1044 Size: 73 475 496 Fragments arranged by size: 496 475 73 BsiHKAI G_wGCw'C Cuts at: 0 857 1044 Size: 857 187 Bsp1286I G_dGCh'C Cuts at: 0 7 209 857 896 1022 1044 Size: 7 202 648 39 126 22 Fragments arranged by size: 648 202 126 39 22 7 BspHI T'CATG_A Cuts at: 0 186 1044 Size: 186 858 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 345 1044 Size: 345 699 BsrFI r'CCGG_y Cuts at: 0 18 1044 Size: 18 1026 BstAPI GCAn_nnn'nTGC Cuts at: 0 579 1044 Size: 579 465 BstBI TT'CG_AA Cuts at: 0 752 1044 Size: 752 292 BstXI CCAn_nnnn'nTGG Cuts at: 0 498 1044 Size: 498 546 BtgI C'CryG_G Cuts at: 0 50 217 1013 1044 Size: 50 167 796 31 Fragments arranged by size: 796 167 50 31 EarI CTCTTCn'nnn_ Cuts at: 0 103 657 821 985 1044 Size: 103 554 164 164 59 Fragments arranged by size: 554 164 164 103 59 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 520 568 604 638 739 1044 Size: 520 48 36 34 101 305 Fragments arranged by size: 520 305 101 48 36 34 EcoO109I rG'GnC_Cy Cuts at: 0 120 482 539 785 1044 Size: 120 362 57 246 259 Fragments arranged by size: 362 259 246 120 57 EcoRI G'AATT_C Cuts at: 0 439 1044 Size: 439 605 HaeII r_GCGC'y Cuts at: 0 25 69 1044 Size: 25 44 975 Fragments arranged by size: 975 44 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 252 284 1044 Size: 252 32 760 Fragments arranged by size: 760 252 32 HincII GTy'rAC Cuts at: 0 300 1044 Size: 300 744 HindIII A'AGCT_T Cuts at: 0 143 1044 Size: 143 901 MslI CAynn'nnrTG Cuts at: 0 706 1044 Size: 706 338 MspA1I CmG'CkG Cuts at: 0 52 168 258 570 1044 Size: 52 116 90 312 474 Fragments arranged by size: 474 312 116 90 52 NaeI GCC'GGC Cuts at: 0 20 1044 Size: 20 1024 NcoI C'CATG_G Cuts at: 0 217 1013 1044 Size: 217 796 31 Fragments arranged by size: 796 217 31 NgoMIV G'CCGG_C Cuts at: 0 18 1044 Size: 18 1026 NspI r_CATG'y Cuts at: 0 291 1044 Size: 291 753 PasI CC'CwG_GG Cuts at: 0 479 820 1044 Size: 479 341 224 Fragments arranged by size: 479 341 224 PciI A'CATG_T Cuts at: 0 287 1044 Size: 287 757 PflMI CCAn_nnn'nTGG Cuts at: 0 485 713 1044 Size: 485 228 331 Fragments arranged by size: 485 331 228 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 984 1016 1044 Size: 984 32 28 Fragments arranged by size: 984 32 28 PpuMI rG'GwC_Cy Cuts at: 0 120 785 1044 Size: 120 665 259 Fragments arranged by size: 665 259 120 PshAI GACnn'nnGTC Cuts at: 0 52 125 1044 Size: 52 73 919 Fragments arranged by size: 919 73 52 PspOMI G'GGCC_C Cuts at: 0 3 1044 Size: 3 1041 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 526 558 1044 Size: 526 32 486 Fragments arranged by size: 526 486 32 PstI C_TGCA'G Cuts at: 0 62 257 356 575 1044 Size: 62 195 99 219 469 Fragments arranged by size: 469 219 195 99 62 PvuII CAG'CTG Cuts at: 0 168 258 570 1044 Size: 168 90 312 474 Fragments arranged by size: 474 312 168 90 SacII CC_GC'GG Cuts at: 0 53 1044 Size: 53 991 SapI GCTCTTCn'nnn_ Cuts at: 0 821 1044 Size: 821 223 SbfI CC_TGCA'GG Cuts at: 0 62 356 1044 Size: 62 294 688 Fragments arranged by size: 688 294 62 SfcI C'TryA_G Cuts at: 0 58 253 352 571 1044 Size: 58 195 99 219 473 Fragments arranged by size: 473 219 195 99 58 SspI AAT'ATT Cuts at: 0 983 1044 Size: 983 61 StuI AGG'CCT Cuts at: 0 863 1044 Size: 863 181 StyI C'CwwG_G Cuts at: 0 123 217 743 806 1013 1044 Size: 123 94 526 63 207 31 Fragments arranged by size: 526 207 123 94 63 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 94 872 1044 Size: 94 778 172 Fragments arranged by size: 778 172 94 Enzymes that do cut: AcuI AflIII AloI AlwNI ApaI ApoI BaeI BanI BanII BbsI BcgI BclI BglI Bme1580I BpmI BsaAI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrFI BstAPI BstBI BstXI BtgI EarI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII HindIII MslI MspA1I NaeI NcoI NgoMIV NspI PasI PciI PflMI PpiI PpuMI PshAI PspOMI PsrI PstI PvuII SacII SapI SbfI SfcI SspI StuI StyI TaqII Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI ApaLI AscI AseI AsiSI AvaI AvrII BamHI BbeI BbvCI BciVI BfrBI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrGI BssHII BssSI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HpaI KasI KpnI MfeI MluI MmeI MscI NarI NdeI NheI NotI NruI NsiI PacI PfoI PmeI PmlI PsiI PspXI PvuI RsrII SacI SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI