Restriction map for
pFN21AE1218
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AhdI Alw44I
AlwI AlwNI AocI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BpmI BpuAI
BsaAI BsaBI BseAI BseRI BsgI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstYI BstZ17I Bsu36I ClaI CpoI
DpnII DraIII DrdI Eam1105I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FokI FseI FspI HincII
HindII HindIII HinfI HpaI KpnI
MaeI MaeII MamI MboI MfeI
MflI MluI MluNI MroI MscI
MunI NcoI NdeI NdeII NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SalI Sau3AI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tsp45I Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AfaI 89 GTAC GT^AC
AgeI 295 ACCGGT A^CCGGT
Aor51HI 448 AGCGCT AGC^GCT
AspHI 124 GAGCTC G[AT]GC[AT]^C
AvaII 109 GGTCC G^G[AT]CC
BbeI 624 GGCGCC GGCGC^C
BglI 73 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 592 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 592 GCTAAGC GC^T[ACGT]AGC
BsaI 247 GGTCTC GGTCTC(1/5)
BsiEI 482 CGGCCG CG[GA][CT]^CG
BsiHKAI 124 GAGCTC G[AT]GC[AT]^C
BsiYI 519 CCCAAAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 519 CCCAAAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 110 GTCCC GGGAC(10/14)
BsrBI 20 CCGCTC CCGCTC(-3/-3)
BsrI 391 CCAGT ACTGG(1/-1)
BstNI 258 CCTGG CC^[AT]GG
CelII 592 GCTAAGC GC^T[ACGT]AGC
DraI 313 TTTAAA TTT^AAA
DraII 50 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 485 CCGCGG C^C[GA][CT]GG
EagI 482 CGGCCG C^GGCCG
EarI 444 GAAGAG CTCTTC(1/4)
EclXI 482 CGGCCG C^GGCCG
Eco47III 448 AGCGCT AGC^GCT
Eco52I 482 CGGCCG C^GGCCG
EcoO109I 50 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRII 258 CCTGG ^CC[AT]GG
HgaI 103 GCGTC GACGC(5/10)
KasI 624 GGCGCC G^GCGCC
Ksp632I 444 GAAGAG CTCTTC(1/4)
KspI 485 CCGCGG CCGC^GG
MaeIII 496 GTAAC ^GT[ACGT]AC
MslI 273 CACTCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 258 CCTGG CC^[AT]GG
NarI 624 GGCGCC GG^CGCC
PinAI 295 ACCGGT A^CCGGT
RsaI 89 GTAC GT^AC
SacI 124 GAGCTC GAGCT^C
SacII 485 CCGCGG CCGC^GG
SapI 444 GAAGAGC GCTCTTC(1/4)
SfoI 624 GGCGCC GGC^GCC
SgrAI 294 CACCGGTG C[GA]^CCGG[CT]G
SmlI 250 CTCAAG C^T[CT][GA]AG
TspRI 492 CACTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
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AccII 33 CGCG CG^CG
486 CGCG
AcyI 102 GGCGTC G[GA]^CG[CT]C
624 GGCGCC
AluI 125 AGCT AG^CT
552 AGCT
BanI 292 GGCACC G^G[CT][GA]CC
624 GGCGCC
BanII 124 GAGCTC G[GA]GC[CT]^C
516 GAGCCC
BcnI 114 CCCGG CC^[GC]GG
338 CCGGG
BsaHI 102 GGCGTC G[GA]^CG[CT]C
624 GGCGCC
BsaWI 106 TCCGGT [AT]^CCGG[AT]
295 ACCGGT
BsmAI 7 GAGAC GTCTC(1/5)
248 GTCTC
BstUI 33 CGCG CG^CG
486 CGCG
BstXI 134 CCAAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
500 CCAAGAAAGTGG
Cfr13I 51 GGCCC G^G[ACGT]CC
109 GGTCC
FauI 25 CCCGC CCCGC(4/6)
367 GCGGG
HaeII 448 AGCGCT [GA]GCGC^[CT]
624 GGCGCC
Hin1I 102 GGCGTC G[GA]^CG[CT]C
624 GGCGCC
HphI 261 GGTGA GGTGA(8/7)
131 TCACC
MseI 314 TTAA T^TAA
359 TTAA
MspA1I 26 CCGCTG C[AC]G^C[GT]G
485 CCGCGG
MvnI 33 CGCG CG^CG
486 CGCG
NaeI 205 GCCGGC GCC^GGC
480 GCCGGC
NciI 114 CCCGG CC^[GC]GG
338 CCGGG
NgoMIV 205 GCCGGC G^CCGGC
480 GCCGGC
Sau96I 51 GGCCC G^G[ACGT]CC
109 GGTCC
Tru9I 314 TTAA T^TAA
359 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI BbvI BmyI BsaJI Bsp1286I
BsrFI BssKI Cac8I CfoI Cfr10I
DdeI EaeI EcoT14I Fnu4HI HaeIII
HapII HhaI HinP1I HpaII ItaI
MboII MnlI MspI MwoI NlaIV
ScrFI StyI TseI