(Circular) (Six-base) MAPSORT of: pFN21AE1218.seq Check: 627 from: 1 to: 5344 pFN21AE1218 5344 bp With 182 enzymes: SgfI * November 11, 2016 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3154 278 Size: 53 83 186 936 1618 2468 Fragments arranged by size: 2468 1618 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2687 1937 Size: 750 4594 Acc65I G'GTAC_C Cuts at: 2665 2665 Size: 5344 AclI AA'CG_TT Cuts at: 1563 3471 3844 1563 Size: 1908 373 3063 Fragments arranged by size: 3063 1908 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3407 4791 5059 1632 Size: 1775 1384 268 1917 Fragments arranged by size: 1917 1775 1384 268 AfeI AGC'GCT Cuts at: 2455 2455 Size: 5344 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5147 AflIII A'CryG_T Cuts at: 1203 4243 5220 1203 Size: 3040 977 1327 Fragments arranged by size: 3040 1327 977 AgeI A'CCGG_T Cuts at: 2300 4175 2300 Size: 1875 3469 AhdI GACnn_n'nnGTC Cuts at: 4073 4073 Size: 5344 AleI CACnn'nnGTG Cuts at: 1166 2282 1166 Size: 1116 4228 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2300 1184 Size: 1116 4228 AlwNI CAG_nnn'CTG Cuts at: 3087 4659 3087 Size: 1572 3772 ApaLI G'TGCA_C Cuts at: 3401 4557 3401 Size: 1156 4188 ApoI r'AATT_y Cuts at: 1493 2653 2879 3031 5140 1493 Size: 1160 226 152 2109 1697 Fragments arranged by size: 2109 1697 1160 226 152 AseI AT'TA_AT Cuts at: 160 1032 3898 160 Size: 872 2866 1606 Fragments arranged by size: 2866 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5344 AvaI C'yCGr_G Cuts at: 1870 1943 2669 1870 Size: 73 726 4545 Fragments arranged by size: 4545 726 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2224 2257 2224 Size: 33 5311 BamHI G'GATC_C Cuts at: 2674 3041 2674 Size: 367 4977 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2297 2629 2665 4120 618 Size: 325 217 627 510 332 36 1455 1842 Fragments arranged by size: 1842 1455 627 510 332 325 217 36 BanII G_rGCy'C Cuts at: 1391 2133 2525 2663 1391 Size: 742 392 138 4072 Fragments arranged by size: 4072 742 392 138 BbeI G_GCGC'C Cuts at: 2633 2633 Size: 5344 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4373 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3535 3569 1903 Size: 34 1598 34 3678 Fragments arranged by size: 3678 1598 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3236 4446 3236 Size: 1210 4134 BclI T'GATC_A Cuts at: 1548 1548 Size: 5344 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2084 3955 136 Size: 107 122 71 631 605 412 1871 1525 Fragments arranged by size: 1871 1525 631 605 412 122 107 71 BglII A'GATC_T Cuts at: 5339 5339 Size: 5344 BlpI GC'TnA_GC Cuts at: 2598 2761 2598 Size: 163 5181 Bme1580I G_kGCm'C Cuts at: 1790 2279 3405 4561 1790 Size: 489 1126 1156 2573 Fragments arranged by size: 2573 1156 1126 489 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5344 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3075 4033 442 Size: 416 53 83 399 24 1658 958 1753 Fragments arranged by size: 1753 1658 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5344 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4004 1239 Size: 2765 2579 Bpu10I CC'TnA_GC Cuts at: 2574 2574 Size: 5344 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2240 3469 4334 4632 2240 Size: 1229 865 298 2952 Fragments arranged by size: 2952 1229 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2258 4007 882 Size: 617 759 1749 2219 Fragments arranged by size: 2219 1749 759 617 BsaAI yAC'GTr Cuts at: 493 1204 3135 493 Size: 711 1931 2702 Fragments arranged by size: 2702 1931 711 BsaBI GATnn'nnATC Cuts at: 5338 5338 Size: 5344 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2108 2630 3151 3533 Size: 53 83 186 936 575 522 521 382 Cuts at: 3533 4168 275 Size: 635 1451 Fragments arranged by size: 1451 936 635 575 522 521 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2111 2300 3777 4175 4449 4596 5007 2111 Size: 189 1477 398 274 147 411 2448 Fragments arranged by size: 2448 1477 411 398 274 189 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5119 Fragments arranged by size: 5119 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5344 BseYI C'CCAG_C Cuts at: 2087 4547 5043 2087 Size: 2460 496 2388 Fragments arranged by size: 2460 2388 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4771 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2490 2794 3555 4583 1540 Size: 275 186 489 304 761 1028 2301 Fragments arranged by size: 2301 1028 761 489 304 275 186 BsiHKAI G_wGCw'C Cuts at: 2133 3405 3490 4561 2133 Size: 1272 85 1071 2916 Fragments arranged by size: 2916 1272 1071 85 BsmI GAATG_Cn' Cuts at: 2855 2948 2855 Size: 93 5251 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2133 2279 2525 2663 3405 3490 4561 Size: 399 343 146 246 138 742 85 1071 Cuts at: 4561 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 343 246 146 138 85 BspEI T'CCGG_A Cuts at: 5007 5007 Size: 5344 BspHI T'CATG_A Cuts at: 3233 4963 3233 Size: 1730 3614 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2699 844 Size: 1855 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2027 3231 1394 Size: 429 204 1204 3507 Fragments arranged by size: 3507 1204 429 204 BsrDI GCAATG_nn' Cuts at: 65 1248 3839 4013 65 Size: 1183 2591 174 1396 Fragments arranged by size: 2591 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2210 2300 2485 3988 4175 1700 Size: 110 400 90 185 1503 187 2869 Fragments arranged by size: 2869 1503 400 187 185 110 90 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5344 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5344 BssSI C'ACGA_G Cuts at: 3404 4416 3404 Size: 1012 4332 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5344 BstBI TT'CG_AA Cuts at: 4148 4148 Size: 5344 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5344 BstXI CCAn_nnnn'nTGG Cuts at: 2146 2512 2146 Size: 366 4978 BstYI r'GATC_y Cuts at: 1972 2674 3041 3426 3443 4895 4981 4993 5339 Size: 702 367 385 17 1452 86 12 346 Cuts at: 5339 1972 Size: 1977 Fragments arranged by size: 1977 1452 702 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2490 513 Size: 552 1425 3367 Fragments arranged by size: 3367 1425 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4605 Fragments arranged by size: 4605 683 56 BtsI GCAGTG_nn' Cuts at: 2860 3654 3674 2860 Size: 794 20 4530 Fragments arranged by size: 4530 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5344 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5309 DraI TTT'AAA Cuts at: 2320 2648 2320 Size: 328 5016 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4351 809 Size: 507 3035 1802 Fragments arranged by size: 3035 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2076 2148 2487 Size: 54 1425 50 275 18 246 72 339 Cuts at: 2487 2493 2791 3680 5309 8 Size: 6 298 889 1629 43 Fragments arranged by size: 1629 1425 889 339 298 275 246 72 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2487 2791 1537 Size: 275 675 304 4090 Fragments arranged by size: 4090 675 304 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2444 3274 1353 Size: 1091 830 3423 Fragments arranged by size: 3423 1091 830 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2391 3931 4305 4451 387 Size: 1064 138 802 1540 374 146 1280 Fragments arranged by size: 1540 1280 1064 802 374 146 138 EcoICRI GAG'CTC Cuts at: 2131 2131 Size: 5344 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3407 4004 4791 5059 1239 Size: 393 1775 597 787 268 1524 Fragments arranged by size: 1775 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2056 2056 Size: 5344 EcoRI G'AATT_C Cuts at: 2653 3031 2653 Size: 378 4966 FspI TGC'GCA Cuts at: 3850 3850 Size: 5344 HaeII r_GCGC'y Cuts at: 1678 2457 2633 4491 1678 Size: 779 176 1858 2531 Fragments arranged by size: 2531 1858 779 176 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3985 4017 4059 4091 1373 Size: 32 135 32 2413 32 42 32 2626 Fragments arranged by size: 2626 2413 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2688 2934 669 Size: 988 281 750 246 3079 Fragments arranged by size: 3079 988 750 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5344 HpaI GTT'AAC Cuts at: 2934 2934 Size: 5344 KasI G'GCGC_C Cuts at: 2629 2629 Size: 5344 KpnI G_GTAC'C Cuts at: 2669 2669 Size: 5344 MfeI C'AATT_G Cuts at: 2943 2943 Size: 5344 MluI A'CGCG_T Cuts at: 5220 5220 Size: 5344 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4458 4642 1305 Size: 3153 184 2007 Fragments arranged by size: 3153 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3522 Fragments arranged by size: 3522 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2282 3302 3661 3820 518 Size: 648 1116 1020 359 159 2042 Fragments arranged by size: 2042 1116 1020 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2033 2492 2760 3437 4585 4830 1606 Size: 427 459 268 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 459 427 268 245 NaeI GCC'GGC Cuts at: 2212 2487 2212 Size: 275 5069 NarI GG'CG_CC Cuts at: 2630 2630 Size: 5344 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4792 NdeI CA'TA_TG Cuts at: 387 387 Size: 5344 NgoMIV G'CCGG_C Cuts at: 2210 2485 2210 Size: 275 5069 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5344 NotI GC'GGCC_GC Cuts at: 2791 2791 Size: 5344 NspI r_CATG'y Cuts at: 1132 1229 2702 4247 1132 Size: 97 1473 1545 2229 Fragments arranged by size: 2229 1545 1473 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5344 PciI A'CATG_T Cuts at: 4243 4243 Size: 5344 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5344 PmeI GTTT'AAAC Cuts at: 2648 2648 Size: 5344 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3413 3445 4926 4958 3413 Size: 32 1481 32 3799 Fragments arranged by size: 3799 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5344 PsiI TTA'TAA Cuts at: 2914 2914 Size: 5344 PstI C_TGCA'G Cuts at: 830 2696 830 Size: 1866 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5344 SacI G_AGCT'C Cuts at: 2133 2133 Size: 5344 SacII CC_GC'GG Cuts at: 2493 2493 Size: 5344 SalI G'TCGA_C Cuts at: 1936 2686 1936 Size: 750 4594 SapI GCTCTTCn'nnn_ Cuts at: 2444 2444 Size: 5344 SbfI CC_TGCA'GG Cuts at: 2696 2696 Size: 5344 ScaI AGT'ACT Cuts at: 3592 5304 3592 Size: 1712 3632 SfcI C'TryA_G Cuts at: 826 1045 2692 3827 4508 4699 826 Size: 219 1647 1135 681 191 1471 Fragments arranged by size: 1647 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2631 2631 Size: 5344 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5344 SgrAI Cr'CCGG_yG Cuts at: 1700 2300 1700 Size: 600 4744 SmaI CCC'GGG Cuts at: 1872 2671 1872 Size: 799 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2255 3448 4349 4611 820 Size: 197 926 312 1193 901 262 1553 Fragments arranged by size: 1553 1193 926 901 312 262 197 SnaBI TAC'GTA Cuts at: 493 3135 493 Size: 2642 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5344 SphI G_CATG'C Cuts at: 1132 2702 1132 Size: 1570 3774 SspI AAT'ATT Cuts at: 5 52 3268 5 Size: 47 3216 2081 Fragments arranged by size: 3216 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5344 StyI C'CwwG_G Cuts at: 513 1065 2139 2358 2535 2589 2604 2783 513 Size: 552 1074 219 177 54 15 179 3074 Fragments arranged by size: 3074 1074 552 219 179 177 54 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3371 3388 3541 3726 4171 325 Size: 1255 1791 17 153 185 445 1498 Fragments arranged by size: 1791 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3590 5302 96 Size: 274 80 33 51 1443 1613 1712 138 Fragments arranged by size: 1712 1613 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5304 5336 5304 Size: 32 5312 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5344 XbaI T'CTAG_A Cuts at: 2680 2680 Size: 5344 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5344 XmaI C'CCGG_G Cuts at: 1870 2669 1870 Size: 799 4545 XmnI GAAnn'nnTTC Cuts at: 3473 3473 Size: 5344 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3152 276 Size: 53 83 186 936 1618 2468 Fragments arranged by size: 2468 1618 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI SexAI SfiI SrfI SwaI XcmI