Restriction map for
pFN21AE1218
(Linear) (Six-base) MAPSORT of: KIEE1218.seq Check: 2247 from: 1 to: 639
KIEE1218.orf
With 182 enzymes: SgfI *
November 11, 2016 16:52 ..
AfeI AGC'GCT
Cuts at: 0 450 639
Size: 450 189
AgeI A'CCGG_T
Cuts at: 0 295 639
Size: 295 344
AleI CACnn'nnGTG
Cuts at: 0 277 639
Size: 277 362
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 295 639
Size: 295 344
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 219 252 639
Size: 219 33 387
Fragments arranged by size:
387 219 33
BanI G'GyrC_C
Cuts at: 0 292 624 639
Size: 292 332 15
Fragments arranged by size:
332 292 15
BanII G_rGCy'C
Cuts at: 0 128 520 639
Size: 128 392 119
Fragments arranged by size:
392 128 119
BbeI G_GCGC'C
Cuts at: 0 628 639
Size: 628 11
BglI GCCn_nnn'nGGC
Cuts at: 0 79 639
Size: 79 560
BlpI GC'TnA_GC
Cuts at: 0 593 639
Size: 593 46
Bme1580I G_kGCm'C
Cuts at: 0 274 639
Size: 274 365
Bpu10I CC'TnA_GC
Cuts at: 0 569 639
Size: 569 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 639
Size: 235 404
BsaI GGTCTCn'nnnn_
Cuts at: 0 253 639
Size: 253 386
BsaHI Gr'CG_yC
Cuts at: 0 103 625 639
Size: 103 522 14
Fragments arranged by size:
522 103 14
BsaWI w'CCGG_w
Cuts at: 0 106 295 639
Size: 106 189 344
Fragments arranged by size:
344 189 106
BseYI C'CCAG_C
Cuts at: 0 82 639
Size: 82 557
BsiEI CG_ry'CG
Cuts at: 0 485 639
Size: 485 154
BsiHKAI G_wGCw'C
Cuts at: 0 128 639
Size: 128 511
Bsp1286I G_dGCh'C
Cuts at: 0 128 274 520 639
Size: 128 146 246 119
Fragments arranged by size:
246 146 128 119
BsrBI CCG'CTC
Cuts at: 0 22 639
Size: 22 617
BsrFI r'CCGG_y
Cuts at: 0 205 295 480 639
Size: 205 90 185 159
Fragments arranged by size:
205 185 159 90
BstXI CCAn_nnnn'nTGG
Cuts at: 0 141 507 639
Size: 141 366 132
Fragments arranged by size:
366 141 132
BtgI C'CryG_G
Cuts at: 0 485 639
Size: 485 154
DraI TTT'AAA
Cuts at: 0 315 639
Size: 315 324
EaeI y'GGCC_r
Cuts at: 0 71 143 482 488 639
Size: 71 72 339 6 151
Fragments arranged by size:
339 151 72 71 6
EagI C'GGCC_G
Cuts at: 0 482 639
Size: 482 157
EarI CTCTTCn'nnn_
Cuts at: 0 439 639
Size: 439 200
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 386 639
Size: 386 253
EcoICRI GAG'CTC
Cuts at: 0 126 639
Size: 126 513
EcoO109I rG'GnC_Cy
Cuts at: 0 51 639
Size: 51 588
HaeII r_GCGC'y
Cuts at: 0 452 628 639
Size: 452 176 11
Fragments arranged by size:
452 176 11
KasI G'GCGC_C
Cuts at: 0 624 639
Size: 624 15
MslI CAynn'nnrTG
Cuts at: 0 277 639
Size: 277 362
MspA1I CmG'CkG
Cuts at: 0 28 487 639
Size: 28 459 152
Fragments arranged by size:
459 152 28
NaeI GCC'GGC
Cuts at: 0 207 482 639
Size: 207 275 157
Fragments arranged by size:
275 207 157
NarI GG'CG_CC
Cuts at: 0 625 639
Size: 625 14
NgoMIV G'CCGG_C
Cuts at: 0 205 480 639
Size: 205 275 159
Fragments arranged by size:
275 205 159
SacI G_AGCT'C
Cuts at: 0 128 639
Size: 128 511
SacII CC_GC'GG
Cuts at: 0 488 639
Size: 488 151
SapI GCTCTTCn'nnn_
Cuts at: 0 439 639
Size: 439 200
SfoI GGC'GCC
Cuts at: 0 626 639
Size: 626 13
SgrAI Cr'CCGG_yG
Cuts at: 0 295 639
Size: 295 344
SmlI C'TyrA_G
Cuts at: 0 250 639
Size: 250 389
StyI C'CwwG_G
Cuts at: 0 134 353 530 584 599 639
Size: 134 219 177 54 15 40
Fragments arranged by size:
219 177 134 54 40 15
Enzymes that do cut:
AfeI AgeI AleI AlfI BaeI BanI BanII BbeI
BglI BlpI Bme1580I Bpu10I BpuEI BsaI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BsrBI BsrFI BstXI BtgI
DraI EaeI EagI EarI EciI EcoICRI EcoO109I HaeII
KasI MslI MspA1I NaeI NarI NgoMIV SacI SacII
SapI SfoI SgrAI SmlI StyI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AhdI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglII BmgBI BmrI BmtI BplI BpmI
BsaAI BsaBI BsaXI BseRI BsgI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraIII DrdI Eco57MI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SalI SanDI SbfI
ScaI SexAI SfcI SfiI SgfI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI