################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII AccI AccII AccIII AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BanII BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BseAI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HpaI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI NaeI NcoI NdeI NgoMIV NlaIII NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 301 GGTACC G^GTACC AclI 282 AACGTT AA^CGTT AfaI 302 GTAC GT^AC Asp718I 301 GGTACC G^GTACC AvaI 312 CTCGGG C^[CT]CG[GA]G BbeI 364 GGCGCC GGCGC^C BcnI 361 CCCGG CC^[GC]GG BglI 424 GCCACGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 304 ACCGGA [AT]^CCGG[AT] BseRI 156 CTCCTC GAGGAG(10/8) BsiEI 209 CGGCCG CG[GA][CT]^CG BsoBI 312 CTCGGG C^[CT]CG[GA]G BspMI 78 ACCTGC ACCTGC(4/8) BstAPI 394 GCAACGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 267 CCTGG CC^[AT]GG Cfr13I 118 GGCCC G^G[ACGT]CC DpnII 138 GATC ^GATC EagI 209 CGGCCG C^GGCCG EclXI 209 CGGCCG C^GGCCG Eco52I 209 CGGCCG C^GGCCG EcoRII 267 CCTGG ^CC[AT]GG HphI 594 GGTGA GGTGA(8/7) KasI 364 GGCGCC G^GCGCC KpnI 301 GGTACC GGTAC^C MaeIII 595 GTGAC ^GT[ACGT]AC MboI 138 GATC ^GATC MboII 231 GAAGA GAAGA(8/7) MspA1I 113 CCGCTG C[AC]G^C[GT]G MvaI 267 CCTGG CC^[AT]GG NarI 364 GGCGCC GG^CGCC NciI 361 CCCGG CC^[GC]GG NdeII 138 GATC ^GATC NheI 433 GCTAGC G^CTAGC NotI 208 GCGGCCGC GC^GGCCGC PleI 453 GACTC GAGTC(4/5) Psp1406I 282 AACGTT AA^CGTT PstI 89 CTGCAG CTGCA^G RsaI 302 GTAC GT^AC Sau3AI 138 GATC ^GATC Sau96I 118 GGCCC G^G[ACGT]CC SfaNI 403 GCATC GCATC(5/9) SfcI 89 CTGCAG C^T[GA][CT]AG SfoI 364 GGCGCC GGC^GCC TfiI 501 GAATC G^A[AT]TC Tsp45I 595 GTGAC ^GT[GC]AC TspRI 11 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcyI 339 GGCGTC G[GA]^CG[CT]C 364 GGCGCC BfaI 434 CTAG C^TAG 590 CTAG BsaHI 339 GGCGTC G[GA]^CG[CT]C 364 GGCGCC BsgI 142 GTGCAG GTGCAG(16/14) 289 GTGCAG BsiYI 52 CCTTTAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 584 CCAAGGCTAGG BslI 52 CCTTTAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 584 CCAAGGCTAGG BssKI 267 CCTGG ^CC[ACGT]GG 361 CCCGG CfoI 202 GCGC GCG^C 365 GCGC DsaI 347 CCGTGG C^C[GA][CT]GG 425 CCACGG EaeI 209 CGGCCG [CT]^GGCC[GA] 581 CGGCCA EcoT14I 575 CCAAGG C^C[AT][AT]GG 584 CCAAGG HaeII 201 GGCGCT [GA]GCGC^[CT] 364 GGCGCC HapII 305 CCGG C^CGG 362 CCGG HgaI 340 GCGTC GACGC(5/10) 446 GCGTC HhaI 202 GCGC GCG^C 365 GCGC Hin1I 339 GGCGTC G[GA]^CG[CT]C 364 GGCGCC HinP1I 202 GCGC G^CGC 365 GCGC HinfI 453 GACTC G^A[ACGT]TC 501 GAATC HpaII 305 CCGG C^CGG 362 CCGG MaeI 434 CTAG C^TAG 590 CTAG MaeII 224 ACGT A^CGT 283 ACGT MspI 305 CCGG C^CGG 362 CCGG ScrFI 267 CCTGG CC^[ACGT]GG 361 CCCGG StyI 575 CCAAGG C^C[AT][AT]GG 584 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 17 enzymes) # ######################################### AciI AluI BanI BbvI BsaJI Cac8I DdeI FauI Fnu4HI HaeIII ItaI MnlI MseI MwoI NlaIV Tru9I TseI