Restriction map for
pFN21AE2715
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspHI AspI AvaI
AviII AvrII BalI BanI BbeI
BbrPI BcgI BciVI BcnI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaHI BsiEI BsiHKAI BsiWI BsmBI
BsoBI BspDI BspHI BspLU11I BspMI
BsrBI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
KasI KpnI KspI MaeIII MamI
MfeI MluI MluNI MscI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI TaqI
Tsp45I Tth111I TthHB8I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 506 TCCGGA T^CCGGA
AciI 481 GCGG CCGC(-3/-1)
AcsI 283 GAATTT [GA]^AATT[CT]
AhdI 11 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 283 GAATTT [GA]^AATT[CT]
Asp700I 1101 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 11 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 992 GGATCC G^GATCC
BbvCI 339 CCTCAGC CCTCAGC(-5/-2)
BclI 863 TGATCA T^GATCA
BfaI 212 CTAG C^TAG
BlpI 817 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 817 GCTCAGC GC^T[ACGT]AGC
BseAI 506 TCCGGA T^CCGGA
BsgI 1453 GTGCAG GTGCAG(16/14)
BsmI 76 GAATGC GAATGC(1/-1)
Bsp1407I 513 TGTACA T^GTACA
BspEI 506 TCCGGA T^CCGGA
BsrGI 513 TGTACA T^GTACA
BssSI 1144 CTCGTG CACGAG(-5/-1)
BstAPI 472 GCAGCTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 410 CCAAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 817 GCTCAGC GC^T[ACGT]AGC
DraII 631 GGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 1440 TGGCCG [CT]^GGCC[GA]
Eam1105I 11 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 631 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 1038 CCTTGG C^C[AT][AT]GG
FauI 481 GCGGG CCCGC(4/6)
FbaI 863 TGATCA T^GATCA
MaeI 212 CTAG C^TAG
MroI 506 TCCGGA T^CCGGA
MslI 1179 CATGACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1397 CAGCTG C[AC]G^C[GT]G
NdeI 305 CATATG CA^TATG
PpuMI 631 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 310 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1397 CAGCTG CAG^CTG
SfcI 26 CTACAG C^T[GA][CT]AG
SspBI 513 TGTACA T^GTACA
SspI 399 AATATT AAT^ATT
StyI 1038 CCTTGG C^C[AT][AT]GG
XmnI 1101 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccI 3 GTCTAC GT^[AC][GT]AC
1046 GTCTAC
AlwNI 1218 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1397 CAGCTGCTG
AvaII 10 GGACC G^G[AT]CC
632 GGTCC
BanII 572 GGGCTC G[GA]GC[CT]^C
1235 GAGCCC
BmyI 572 GGGCTC G[AGT]GC[ACT]^C
1235 GAGCCC
BsaI 658 GGTCTC GGTCTC(1/5)
842 GGTCTC
BsaWI 7 ACCGGA [AT]^CCGG[AT]
506 TCCGGA
BseRI 661 CTCCTC GAGGAG(10/8)
1262 CTCCTC
BsmFI 35 GGGAC GGGAC(10/14)
1149 GTCCC
Bsp1286I 572 GGGCTC G[AGT]GC[ACT]^C
1235 GAGCCC
BstYI 992 GGATCC [GA]^GATC[CT]
1165 AGATCC
DraI 57 TTTAAA TTT^AAA
286 TTTAAA
HapII 8 CCGG C^CGG
507 CCGG
HpaI 1243 GTTAAC GTT^AAC
1405 GTTAAC
HpaII 8 CCGG C^CGG
507 CCGG
MflI 992 GGATCC [GA]^GATC[CT]
1165 AGATCC
MspI 8 CCGG C^CGG
507 CCGG
PflMI 528 CCAGGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
674 CCATAGGATGG
SmlI 68 CTCAAG C^T[CT][GA]AG
168 CTTGAG
Van91I 528 CCAGGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
674 CCATAGGATGG
XhoII 992 GGATCC [GA]^GATC[CT]
1165 AGATCC
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# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AfaI AluI AlwI BbsI BbvI
BpmI BpuAI BsaJI BsiYI BslI
BsmAI BsrDI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI TfiI Tru9I TseI Tsp509I
TspRI XcmI