################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraII DrdI DsaI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AccII 736 CGCG CG^CG AcyI 37 GACGCC G[GA]^CG[CT]C AfaI 146 GTAC GT^AC AflIII 128 ACATGT A^C[GA][CT]GT AlwI 507 GGATC GGATC(4/5) AlwNI 377 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 65 AGCGCT AGC^GCT Asp700I 274 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 385 GAGCTC G[AT]GC[AT]^C AspI 154 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 11 CCCGGG C^[CT]CG[GA]G AvaII 720 GGACC G^G[AT]CC BanI 463 GGCACC G^G[CT][GA]CC BlpI 382 GCTGAGC GC^T[ACGT]AGC Bpu1102I 382 GCTGAGC GC^T[ACGT]AGC BsaHI 37 GACGCC G[GA]^CG[CT]C BsaI 404 GAGACC GGTCTC(1/5) BsiHKAI 385 GAGCTC G[AT]GC[AT]^C BsmAI 404 GAGAC GTCTC(1/5) BsmFI 599 GGGAC GGGAC(10/14) BsmI 230 GCATTC GAATGC(1/-1) BsoBI 11 CCCGGG C^[CT]CG[GA]G BspHI 161 TCATGA T^CATGA BspLU11I 128 ACATGT A^CATGT BspMI 54 GCAGGT ACCTGC(4/8) BstNI 648 CCTGG CC^[AT]GG BstUI 736 CGCG CG^CG BstYI 507 GGATCT [GA]^GATC[CT] CelII 382 GCTGAGC GC^T[ACGT]AGC DpnII 508 GATC ^GATC DraI 562 TTTAAA TTT^AAA DraIII 178 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 65 AGCGCT AGC^GCT EcoRII 648 CCTGG ^CC[AT]GG FauI 713 GCGGG CCCGC(4/6) Hin1I 37 GACGCC G[GA]^CG[CT]C MaeII 761 ACGT A^CGT MboI 508 GATC ^GATC MflI 507 GGATCT [GA]^GATC[CT] MseI 563 TTAA T^TAA MvaI 648 CCTGG CC^[AT]GG MvnI 736 CGCG CG^CG NdeII 508 GATC ^GATC NspI 128 ACATGT [GA]CATG^[CT] PflFI 154 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 21 GACTC GAGTC(4/5) PvuII 377 CAGCTG CAG^CTG RcaI 161 TCATGA T^CATGA RsaI 146 GTAC GT^AC SacI 385 GAGCTC GAGCT^C SapI 535 GCTCTTC GCTCTTC(1/4) Sau3AI 508 GATC ^GATC SmaI 11 CCCGGG CCC^GGG TaqI 110 TCGA T^CGA Tru9I 563 TTAA T^TAA Tsp45I 752 GTCAC ^GT[GC]AC Tth111I 154 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 110 TCGA T^CGA XhoII 507 GGATCT [GA]^GATC[CT] XmaCI 11 CCCGGG C^CCGGG XmaI 11 CCCGGG C^CCGGG XmnI 274 GAAAACTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcsI 171 AAATTT [GA]^AATT[CT] 779 AAATTC ApoI 171 AAATTT [GA]^AATT[CT] 779 AAATTC BanII 385 GAGCTC G[GA]GC[CT]^C 644 GAGCCC BmyI 385 GAGCTC G[AGT]GC[ACT]^C 644 GAGCCC BsaJI 11 CCCGGG C^C[ACGT][ACGT]GG 647 CCCTGG Bsp1286I 385 GAGCTC G[AGT]GC[ACT]^C 644 GAGCCC Cfr13I 655 GGCCC G^G[ACGT]CC 720 GGACC EarI 536 CTCTTC CTCTTC(1/4) 97 GAAGAG HaeII 29 GGCGCT [GA]GCGC^[CT] 65 AGCGCT HaeIII 429 GGCC GG^CC 655 GGCC HapII 12 CCGG C^CGG 297 CCGG HpaII 12 CCGG C^CGG 297 CCGG HphI 183 GGTGA GGTGA(8/7) 417 TCACC Ksp632I 536 CTCTTC CTCTTC(1/4) 97 GAAGAG MaeIII 89 GTTAC ^GT[ACGT]AC 752 GTCAC MspI 12 CCGG C^CGG 297 CCGG Sau96I 655 GGCCC G^G[ACGT]CC 720 GGACC TfiI 528 GATTC G^A[AT]TC 741 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AluI BbvI BcnI BsiYI BslI BsrI BssKI BstF5I Cac8I CfoI DdeI Fnu4HI FokI HgaI HhaI HinP1I HinfI ItaI MboII MnlI MslI MspA1I MwoI NciI NlaIII NlaIV ScrFI SfaNI TseI Tsp509I TspRI