(Circular) (Six-base) MAPSORT of: pFN21AE2458.seq Check: 8069 from: 1 to: 5533 pFN21AE2458 5533 bp With 182 enzymes: SgfI * February 8, 2013 19:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3343 278 Size: 53 83 186 936 1807 2468 Fragments arranged by size: 2468 1807 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2876 1937 Size: 939 4594 Acc65I G'GTAC_C Cuts at: 2854 2854 Size: 5533 AclI AA'CG_TT Cuts at: 1563 3660 4033 1563 Size: 2097 373 3063 Fragments arranged by size: 3063 2097 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2528 2610 3596 4980 5248 1632 Size: 896 82 986 1384 268 1917 Fragments arranged by size: 1917 1384 986 896 268 82 AfeI AGC'GCT Cuts at: 2072 2072 Size: 5533 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5336 AflIII A'CryG_T Cuts at: 1203 2133 4432 5409 1203 Size: 930 2299 977 1327 Fragments arranged by size: 2299 1327 977 930 AgeI A'CCGG_T Cuts at: 4364 4364 Size: 5533 AhdI GACnn_n'nnGTC Cuts at: 4262 4262 Size: 5533 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5533 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5533 AlwNI CAG_nnn'CTG Cuts at: 2387 3276 4848 2387 Size: 889 1572 3072 Fragments arranged by size: 3072 1572 889 ApaLI G'TGCA_C Cuts at: 3590 4746 3590 Size: 1156 4377 ApoI r'AATT_y Cuts at: 1493 2176 2784 2842 3068 3220 5329 1493 Size: 683 608 58 226 152 2109 1697 Fragments arranged by size: 2109 1697 683 608 226 152 58 AseI AT'TA_AT Cuts at: 160 1032 4087 160 Size: 872 3055 1606 Fragments arranged by size: 3055 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5533 AvaI C'yCGr_G Cuts at: 1870 1943 2016 2858 1870 Size: 73 73 842 4545 Fragments arranged by size: 4545 842 73 73 BamHI G'GATC_C Cuts at: 2863 3230 2863 Size: 367 5166 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2468 2854 4309 618 Size: 325 217 627 681 386 1455 1842 Fragments arranged by size: 1842 1455 681 627 386 325 217 BanII G_rGCy'C Cuts at: 1391 2394 2653 2852 1391 Size: 1003 259 199 4072 Fragments arranged by size: 4072 1003 259 199 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4562 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3724 3758 1903 Size: 34 1787 34 3678 Fragments arranged by size: 3678 1787 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3425 4635 3425 Size: 1210 4323 BclI T'GATC_A Cuts at: 1548 1548 Size: 5533 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4144 136 Size: 107 122 71 631 605 2472 1525 Fragments arranged by size: 2472 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5528 5528 Size: 5533 BlpI GC'TnA_GC Cuts at: 2388 2950 2388 Size: 562 4971 Bme1580I G_kGCm'C Cuts at: 1790 3594 4750 1790 Size: 1804 1156 2573 Fragments arranged by size: 2573 1804 1156 BmgBI CAC'GTC Cuts at: 1318 2767 1318 Size: 1449 4084 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2156 2825 3264 Size: 416 53 83 399 24 739 669 439 Cuts at: 3264 4222 442 Size: 958 1753 Fragments arranged by size: 1753 958 739 669 439 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5533 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4193 1239 Size: 2954 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3658 4523 4821 3658 Size: 865 298 4370 Fragments arranged by size: 4370 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2403 4196 882 Size: 617 904 1793 2219 Fragments arranged by size: 2219 1793 904 617 BsaAI yAC'GTr Cuts at: 493 1204 3324 493 Size: 711 2120 2702 Fragments arranged by size: 2702 2120 711 BsaBI GATnn'nnATC Cuts at: 5527 5527 Size: 5533 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2043 3340 3722 4357 Size: 53 83 186 936 510 1297 382 635 Cuts at: 4357 275 Size: 1451 Fragments arranged by size: 1451 1297 936 635 510 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3966 4364 4638 4785 5196 3966 Size: 398 274 147 411 4303 Fragments arranged by size: 4303 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5308 Fragments arranged by size: 5308 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5533 BseYI C'CCAG_C Cuts at: 2708 4736 5232 2708 Size: 2028 496 3009 Fragments arranged by size: 3009 2028 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4960 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2983 3744 4772 1540 Size: 275 186 982 761 1028 2301 Fragments arranged by size: 2301 1028 982 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2394 3594 3679 4750 2394 Size: 1200 85 1071 3177 Fragments arranged by size: 3177 1200 1071 85 BsmI GAATG_Cn' Cuts at: 2235 3044 3137 2235 Size: 809 93 4631 Fragments arranged by size: 4631 809 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2394 2653 2852 3594 3679 4750 1391 Size: 399 604 259 199 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 604 399 259 199 85 BspEI T'CCGG_A Cuts at: 5196 5196 Size: 5533 BspHI T'CATG_A Cuts at: 2166 3422 5152 2166 Size: 1256 1730 2547 Fragments arranged by size: 2547 1730 1256 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2050 2888 844 Size: 1206 838 3489 Fragments arranged by size: 3489 1206 838 BsrBI CCG'CTC Cuts at: 1394 1823 3420 1394 Size: 429 1597 3507 Fragments arranged by size: 3507 1597 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4028 4202 65 Size: 1183 2780 174 1396 Fragments arranged by size: 2780 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4177 4364 1700 Size: 110 2367 187 2869 Fragments arranged by size: 2869 2367 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5533 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5533 BssSI C'ACGA_G Cuts at: 3593 4605 3593 Size: 1012 4521 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5533 BstBI TT'CG_AA Cuts at: 4337 4337 Size: 5533 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5533 BstYI r'GATC_y Cuts at: 1972 2512 2863 3230 3615 3632 5084 5170 5182 Size: 540 351 367 385 17 1452 86 12 Cuts at: 5182 5528 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 540 385 367 351 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4042 Fragments arranged by size: 4042 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4794 Fragments arranged by size: 4794 683 56 BtsI GCAGTG_nn' Cuts at: 2374 2758 3049 3843 3863 2374 Size: 384 291 794 20 4044 Fragments arranged by size: 4044 794 384 291 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5533 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5498 DraI TTT'AAA Cuts at: 2569 2837 2569 Size: 268 5265 DraIII CAC_nnn'GTG Cuts at: 2188 2188 Size: 5533 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4540 809 Size: 507 3224 1802 Fragments arranged by size: 3224 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2980 3869 5498 Size: 54 1425 50 275 18 1150 889 1629 Cuts at: 5498 8 Size: 43 Fragments arranged by size: 1629 1425 1150 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2980 1537 Size: 275 1168 4090 Fragments arranged by size: 4090 1168 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2097 2547 3463 1353 Size: 744 450 916 3423 Fragments arranged by size: 3423 916 744 450 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4120 4494 4640 387 Size: 1064 138 2531 374 146 1280 Fragments arranged by size: 2531 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2392 2392 Size: 5533 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2528 2610 3596 4193 4980 5248 1239 Size: 393 896 82 986 597 787 268 1524 Fragments arranged by size: 1524 986 896 787 597 393 268 82 EcoRI G'AATT_C Cuts at: 2842 3220 2842 Size: 378 5155 FspI TGC'GCA Cuts at: 4039 4039 Size: 5533 HaeII r_GCGC'y Cuts at: 1678 2038 2074 4680 1678 Size: 360 36 2606 2531 Fragments arranged by size: 2606 2531 360 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2524 2556 4174 4206 4248 Size: 32 135 32 952 32 1618 32 42 Cuts at: 4248 4280 1373 Size: 32 2626 Fragments arranged by size: 2626 1618 952 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2877 3123 669 Size: 988 281 939 246 3079 Fragments arranged by size: 3079 988 939 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5533 HpaI GTT'AAC Cuts at: 3123 3123 Size: 5533 KpnI G_GTAC'C Cuts at: 2858 2858 Size: 5533 MfeI C'AATT_G Cuts at: 3132 3132 Size: 5533 MluI A'CGCG_T Cuts at: 5409 5409 Size: 5533 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2490 4647 4831 1305 Size: 1185 2157 184 2007 Fragments arranged by size: 2157 2007 1185 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3711 Fragments arranged by size: 3711 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2138 2165 2240 2474 3491 3850 4009 Size: 648 972 27 75 234 1017 359 159 Cuts at: 4009 518 Size: 2042 Fragments arranged by size: 2042 1017 972 648 359 234 159 75 27 MspA1I CmG'CkG Cuts at: 1606 2048 2384 2718 2949 3626 4774 5019 1606 Size: 442 336 334 231 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 442 336 334 245 231 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4042 Fragments arranged by size: 4042 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5533 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5533 NotI GC'GGCC_GC Cuts at: 2980 2980 Size: 5533 NspI r_CATG'y Cuts at: 1132 1229 2137 2891 4436 1132 Size: 97 908 754 1545 2229 Fragments arranged by size: 2229 1545 908 754 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5533 PciI A'CATG_T Cuts at: 2133 4432 2133 Size: 2299 3234 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5533 PmeI GTTT'AAAC Cuts at: 2837 2837 Size: 5533 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3602 3634 5115 5147 3602 Size: 32 1481 32 3988 Fragments arranged by size: 3988 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5533 PsiI TTA'TAA Cuts at: 3103 3103 Size: 5533 PstI C_TGCA'G Cuts at: 830 2885 830 Size: 2055 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5533 PvuII CAG'CTG Cuts at: 2384 2384 Size: 5533 SacI G_AGCT'C Cuts at: 2394 2394 Size: 5533 SalI G'TCGA_C Cuts at: 1936 2875 1936 Size: 939 4594 SapI GCTCTTCn'nnn_ Cuts at: 2547 2547 Size: 5533 SbfI CC_TGCA'GG Cuts at: 2885 2885 Size: 5533 ScaI AGT'ACT Cuts at: 3781 5493 3781 Size: 1712 3821 SfcI C'TryA_G Cuts at: 826 1045 2881 4016 4697 4888 826 Size: 219 1836 1135 681 191 1471 Fragments arranged by size: 1836 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5533 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5533 SmaI CCC'GGG Cuts at: 1872 2018 2860 1872 Size: 146 842 4545 Fragments arranged by size: 4545 842 146 SmlI C'TyrA_G Cuts at: 820 1017 1943 3637 4538 4800 820 Size: 197 926 1694 901 262 1553 Fragments arranged by size: 1694 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3324 493 Size: 2831 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5533 SphI G_CATG'C Cuts at: 1132 2891 1132 Size: 1759 3774 SspI AAT'ATT Cuts at: 5 52 3457 5 Size: 47 3405 2081 Fragments arranged by size: 3405 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5533 StyI C'CwwG_G Cuts at: 513 1065 2004 2972 513 Size: 552 939 968 3074 Fragments arranged by size: 3074 968 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3560 3577 3730 3915 4360 325 Size: 1255 1980 17 153 185 445 1498 Fragments arranged by size: 1980 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3779 5491 96 Size: 274 80 33 51 1443 1802 1712 138 Fragments arranged by size: 1802 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5493 5525 5493 Size: 32 5501 Tth111I GACn'n_nGTC Cuts at: 1316 2162 1316 Size: 846 4687 XbaI T'CTAG_A Cuts at: 2869 2869 Size: 5533 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5533 XmaI C'CCGG_G Cuts at: 1870 2016 2858 1870 Size: 146 842 4545 Fragments arranged by size: 4545 842 146 XmnI GAAnn'nnTTC Cuts at: 2283 3662 2283 Size: 1379 4154 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3341 276 Size: 53 83 186 936 1807 2468 Fragments arranged by size: 2468 1807 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI