Restriction map for
pFN21AE4583
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BbeI BbrPI BcgI BciVI
BfrI BlnI BsaHI BsaWI BseAI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
Bst1107I BstBI BstZ17I Cfr10I ClaI
CpoI DraI DraII DrdI Eam1105I
EarI Eco47III EcoO109I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
Hin1I KasI KpnI Ksp632I KspI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 1459 GTCTAC GT^[AC][GT]AC
BalI 1959 TGGCCA TGG^CCA
BamHI 1316 GGATCC G^GATCC
BanI 1447 GGTGCC G^G[CT][GA]CC
BbvCI 1157 CCTCAGC CCTCAGC(-5/-2)
BclI 32 TGATCA T^GATCA
BglI 1217 GCCGCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 561 AGATCT A^GATCT
BlpI 2115 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2115 GCTCAGC GC^T[ACGT]AGC
BsaBI 172 GATGCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 2164 CTGCAC GTGCAG(16/14)
BsiEI 2265 CGGCCG CG[GA][CT]^CG
BsiWI 2015 CGTACG C^GTACG
BsmBI 1092 CGTCTC CGTCTC(1/5)
BspMI 830 ACCTGC ACCTGC(4/8)
BssSI 1192 CTCGTG CACGAG(-5/-1)
BstAPI 2103 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1186 GGTGACC G^GT[ACGT]ACC
BstPI 1186 GGTGACC G^GT[ACGT]ACC
BstXI 1814 CCAGCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2115 GCTCAGC GC^T[ACGT]AGC
EagI 2265 CGGCCG C^GGCCG
EclXI 2265 CGGCCG C^GGCCG
Eco52I 2265 CGGCCG C^GGCCG
EcoO65I 1186 GGTGACC G^GT[ACGT]ACC
FbaI 32 TGATCA T^GATCA
HindIII 795 AAGCTT A^AGCTT
HpaI 139 GTTAAC GTT^AAC
MamI 172 GATGCGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1959 TGGCCA TGG^CCA
MscI 1959 TGGCCA TGG^CCA
NcoI 431 CCATGG C^CATGG
NheI 666 GCTAGC G^CTAGC
PstI 1556 CTGCAG CTGCA^G
SexAI 1611 ACCAGGT A^CC[AT]GGT
SnaBI 2013 TACGTA TAC^GTA
SspI 778 AATATT AAT^ATT
TaqI 594 TCGA T^CGA
TthHB8I 594 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 1243 CGCG CG^CG
2100 CGCG
AflIII 84 ACACGT A^C[GA][CT]GT
2024 ACGTGT
AlwI 1317 GATCC GGATC(4/5)
1316 GGATC
AlwNI 819 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
2104 CAGCCTCTG
BsaI 2132 GAGACC GGTCTC(1/5)
2293 GAGACC
BseRI 491 GAGGAG GAGGAG(10/8)
974 GAGGAG
BsmI 1809 GAATGC GAATGC(1/-1)
2038 GCATTC
BstUI 1243 CGCG CG^CG
2100 CGCG
BstYI 561 AGATCT [GA]^GATC[CT]
1316 GGATCC
DraIII 65 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
1610 CACCAGGTG
DsaI 431 CCATGG C^C[GA][CT]GG
1403 CCGTGG
EcoNI 1167 CCTCAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1296 CCTGGACAAGG
HgaI 539 GACGC GACGC(5/10)
2098 GACGC
MflI 561 AGATCT [GA]^GATC[CT]
1316 GGATCC
MvnI 1243 CGCG CG^CG
2100 CGCG
NspI 856 GCATGT [GA]CATG^[CT]
2078 GCATGT
PvuII 390 CAGCTG CAG^CTG
1160 CAGCTG
SmlI 2169 CTTGAG C^T[CT][GA]AG
2391 CTCAAG
XhoII 561 AGATCT [GA]^GATC[CT]
1316 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AluI AocI
ApoI Asp700I AspHI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII EaeI Eco81I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XmnI