(Circular) (Six-base) MAPSORT of: pFN21AE4583.seq Check: 3446 from: 1 to: 7225 pFN21AE4583 7225 bp With 182 enzymes: SgfI * March 28, 2012 14:18 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5035 278 Size: 53 83 186 936 3499 2468 Fragments arranged by size: 3499 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3465 4568 1937 Size: 1528 1103 4594 Fragments arranged by size: 4594 1528 1103 Acc65I G'GTAC_C Cuts at: 4546 4546 Size: 7225 AclI AA'CG_TT Cuts at: 1563 5352 5725 1563 Size: 3789 373 3063 Fragments arranged by size: 3789 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2108 4114 4407 5288 6672 6940 1632 Size: 476 2006 293 881 1384 268 1917 Fragments arranged by size: 2006 1917 1384 881 476 293 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7028 AflIII A'CryG_T Cuts at: 1203 2089 4029 6124 7101 1203 Size: 886 1940 2095 977 1327 Fragments arranged by size: 2095 1940 1327 977 886 AgeI A'CCGG_T Cuts at: 6056 6056 Size: 7225 AhdI GACnn_n'nnGTC Cuts at: 5954 5954 Size: 7225 AleI CACnn'nnGTG Cuts at: 1166 4176 1166 Size: 3010 4215 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7225 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2409 2441 2409 Size: 32 7193 AlwNI CAG_nnn'CTG Cuts at: 2829 4114 4968 6540 2829 Size: 1285 854 1572 3514 Fragments arranged by size: 3514 1572 1285 854 ApaLI G'TGCA_C Cuts at: 5282 6438 5282 Size: 1156 6069 ApoI r'AATT_y Cuts at: 1493 2240 2519 2577 2923 2998 4534 4760 4912 Size: 747 279 58 346 75 1536 226 152 Cuts at: 4912 7021 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1536 747 346 279 226 152 75 58 AseI AT'TA_AT Cuts at: 160 1032 5779 160 Size: 872 4747 1606 Fragments arranged by size: 4747 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7225 AvaI C'yCGr_G Cuts at: 1870 1943 4550 1870 Size: 73 2607 4545 Fragments arranged by size: 4545 2607 73 BamHI G'GATC_C Cuts at: 3321 4555 4922 3321 Size: 1234 367 5624 Fragments arranged by size: 5624 1234 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3452 4546 6001 618 Size: 325 217 627 1665 1094 1455 1842 Fragments arranged by size: 1842 1665 1455 1094 627 325 217 BanII G_rGCy'C Cuts at: 1391 2113 2894 3418 3431 3803 4015 4412 4483 Size: 722 781 524 13 372 212 397 71 Cuts at: 4483 4544 1391 Size: 61 4072 Fragments arranged by size: 4072 781 722 524 397 372 212 71 61 13 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3486 3735 4272 928 Size: 971 1587 249 537 3881 Fragments arranged by size: 3881 1587 971 537 249 BbvCI CC'TCA_GC Cuts at: 3163 3163 Size: 7225 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5416 5450 1903 Size: 34 3479 34 3678 Fragments arranged by size: 3678 3479 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5117 6327 5117 Size: 1210 6015 BclI T'GATC_A Cuts at: 1548 2037 1548 Size: 489 6736 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3228 5836 136 Size: 107 122 71 631 605 1556 2608 1525 Fragments arranged by size: 2608 1556 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2566 7220 2566 Size: 4654 2571 BlpI GC'TnA_GC Cuts at: 4121 4642 4121 Size: 521 6704 Bme1580I G_kGCm'C Cuts at: 1790 3189 4498 5286 6442 1790 Size: 1399 1309 788 1156 2573 Fragments arranged by size: 2573 1399 1309 1156 788 BmgBI CAC'GTC Cuts at: 1318 3097 4030 4504 1318 Size: 1779 933 474 4039 Fragments arranged by size: 4039 1779 933 474 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2582 3953 4956 Size: 416 53 83 399 24 1165 1371 1003 Cuts at: 4956 5914 442 Size: 958 1753 Fragments arranged by size: 1753 1371 1165 1003 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2675 1056 Size: 1619 5606 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4502 4502 Size: 7225 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2135 3085 3942 5885 1239 Size: 896 950 857 1943 2579 Fragments arranged by size: 2579 1943 950 896 857 Bpu10I CC'TnA_GC Cuts at: 3109 3163 3671 3109 Size: 54 508 6663 Fragments arranged by size: 6663 508 54 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4195 4381 5350 6215 6513 4195 Size: 186 969 865 298 4907 Fragments arranged by size: 4907 969 865 298 186 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4131 4292 5888 882 Size: 617 2632 161 1596 2219 Fragments arranged by size: 2632 2219 1596 617 161 BsaAI yAC'GTr Cuts at: 493 1204 2092 3970 4020 5016 493 Size: 711 888 1878 50 996 2702 Fragments arranged by size: 2702 1878 996 888 711 50 BsaBI GATnn'nnATC Cuts at: 2181 7219 2181 Size: 5038 2187 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5032 5414 6049 275 Size: 53 83 186 936 3499 382 635 1451 Fragments arranged by size: 3499 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5658 6056 6330 6477 6888 5658 Size: 398 274 147 411 5995 Fragments arranged by size: 5995 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2487 2517 1178 Size: 30 165 30 1084 30 5886 Fragments arranged by size: 5886 1084 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2511 2994 1186 Size: 1325 483 5417 Fragments arranged by size: 5417 1325 483 BseYI C'CCAG_C Cuts at: 3150 3665 3826 6428 6924 3150 Size: 515 161 2602 496 3451 Fragments arranged by size: 3451 2602 515 496 161 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4154 1163 Size: 573 2418 4234 Fragments arranged by size: 4234 2418 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4273 4675 5436 6464 1540 Size: 275 186 2272 402 761 1028 2301 Fragments arranged by size: 2301 2272 1028 761 402 275 186 BsiHKAI G_wGCw'C Cuts at: 2113 2264 3418 4412 5286 5371 6442 2113 Size: 151 1154 994 874 85 1071 2896 Fragments arranged by size: 2896 1154 1071 994 874 151 85 BsiWI C'GTAC_G Cuts at: 4020 4020 Size: 7225 BsmI GAATG_Cn' Cuts at: 3820 4043 4736 4829 3820 Size: 223 693 93 6216 Fragments arranged by size: 6216 693 223 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3103 3103 Size: 7225 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2113 2264 2894 3189 3418 3431 3803 Size: 399 323 151 630 295 229 13 372 Cuts at: 3803 4015 4412 4483 4498 4544 5286 5371 6442 Size: 212 397 71 15 46 742 85 1071 Cuts at: 6442 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 630 399 397 372 323 295 229 212 151 85 71 46 15 13 BspEI T'CCGG_A Cuts at: 6888 6888 Size: 7225 BspHI T'CATG_A Cuts at: 5114 6844 5114 Size: 1730 5495 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2844 4580 844 Size: 2000 1736 3489 Fragments arranged by size: 3489 2000 1736 BsrBI CCG'CTC Cuts at: 1394 1823 5112 1394 Size: 429 3289 3507 Fragments arranged by size: 3507 3289 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2856 3162 3274 3939 4452 5720 5894 Size: 1183 1608 306 112 665 513 1268 174 Cuts at: 5894 65 Size: 1396 Fragments arranged by size: 1608 1396 1268 1183 665 513 306 174 112 BsrFI r'CCGG_y Cuts at: 1700 1810 5869 6056 1700 Size: 110 4059 187 2869 Fragments arranged by size: 4059 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7225 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7225 BssSI C'ACGA_G Cuts at: 3197 5285 6297 3197 Size: 2088 1012 4125 Fragments arranged by size: 4125 2088 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4114 794 Size: 3320 3905 BstBI TT'CG_AA Cuts at: 6029 6029 Size: 7225 BstEII G'GTnAC_C Cuts at: 1184 3191 1184 Size: 2007 5218 BstXI CCAn_nnnn'nTGG Cuts at: 3826 3826 Size: 7225 BstYI r'GATC_y Cuts at: 1972 2566 3321 4555 4922 5307 5324 6776 6862 Size: 594 755 1234 367 385 17 1452 86 Cuts at: 6862 6874 7220 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1234 755 594 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2429 3343 4226 2429 Size: 914 883 5428 Fragments arranged by size: 5428 914 883 BtgI C'CryG_G Cuts at: 513 1065 2436 3408 513 Size: 552 1371 972 4330 Fragments arranged by size: 4330 1371 972 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6486 Fragments arranged by size: 6486 683 56 BtsI GCAGTG_nn' Cuts at: 4741 5535 5555 4741 Size: 794 20 6411 Fragments arranged by size: 6411 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7225 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7190 DraI TTT'AAA Cuts at: 4529 4529 Size: 7225 DraIII CAC_nnn'GTG Cuts at: 2075 3620 2075 Size: 1545 5680 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6232 809 Size: 507 4916 1802 Fragments arranged by size: 4916 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2869 3229 3405 Size: 54 1425 50 275 18 1039 360 176 Cuts at: 3405 3898 3964 4270 4672 5561 7190 8 Size: 493 66 306 402 889 1629 43 Fragments arranged by size: 1629 1425 1039 889 493 402 360 306 275 176 66 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4270 4672 1537 Size: 275 2458 402 4090 Fragments arranged by size: 4090 2458 402 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5155 1353 Size: 3802 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4086 5812 6186 6332 387 Size: 1064 138 2497 1726 374 146 1280 Fragments arranged by size: 2497 1726 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2111 3416 4410 2111 Size: 1305 994 4926 Fragments arranged by size: 4926 1305 994 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2135 3085 3942 4114 4407 5288 Size: 393 476 27 950 857 172 293 881 Cuts at: 5288 5885 6672 6940 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 950 881 857 787 597 476 393 293 268 172 27 EcoNI CCTnn'n_nnAGG Cuts at: 3176 3305 3176 Size: 129 7096 EcoRI G'AATT_C Cuts at: 4534 4912 4534 Size: 378 6847 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2444 4488 2444 Size: 2044 5181 FspI TGC'GCA Cuts at: 5731 5731 Size: 7225 HaeII r_GCGC'y Cuts at: 1678 6372 1678 Size: 4694 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3005 3037 4016 4048 4229 Size: 32 135 32 1433 32 979 32 181 Cuts at: 4229 4261 5866 5898 5940 5972 1373 Size: 32 1605 32 42 32 2626 Fragments arranged by size: 2626 1605 1433 979 181 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2146 2953 3292 4569 4815 669 Size: 988 281 208 807 339 1277 246 3079 Fragments arranged by size: 3079 1277 988 807 339 281 246 208 HindIII A'AGCT_T Cuts at: 748 2800 748 Size: 2052 5173 HpaI GTT'AAC Cuts at: 2146 4815 2146 Size: 2669 4556 KpnI G_GTAC'C Cuts at: 4550 4550 Size: 7225 MfeI C'AATT_G Cuts at: 4824 4824 Size: 7225 MluI A'CGCG_T Cuts at: 7101 7101 Size: 7225 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2790 3354 3988 6339 6523 1305 Size: 1485 564 634 2351 184 2007 Fragments arranged by size: 2351 2007 1485 634 564 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3966 10 Size: 54 1425 343 2134 3269 Fragments arranged by size: 3269 2134 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2175 4082 4176 4421 5183 5542 5701 Size: 648 1009 1907 94 245 762 359 159 Cuts at: 5701 518 Size: 2042 Fragments arranged by size: 2042 1907 1009 762 648 359 245 159 94 MspA1I CmG'CkG Cuts at: 1606 2397 3167 4134 4641 5318 6466 6711 1606 Size: 791 770 967 507 677 1148 245 2120 Fragments arranged by size: 2120 1148 967 791 770 677 507 245 NcoI C'CATG_G Cuts at: 513 1065 2436 513 Size: 552 1371 5302 Fragments arranged by size: 5302 1371 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7225 NheI G'CTAG_C Cuts at: 1052 2671 1052 Size: 1619 5606 NotI GC'GGCC_GC Cuts at: 4672 4672 Size: 7225 NspI r_CATG'y Cuts at: 1132 1229 2865 4087 4583 6128 1132 Size: 97 1636 1222 496 1545 2229 Fragments arranged by size: 2229 1636 1545 1222 496 97 PasI CC'CwG_GG Cuts at: 1348 2325 2887 1348 Size: 977 562 5686 Fragments arranged by size: 5686 977 562 PciI A'CATG_T Cuts at: 6124 6124 Size: 7225 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7225 PfoI T'CCnGG_A Cuts at: 3002 3002 Size: 7225 PmeI GTTT'AAAC Cuts at: 4529 4529 Size: 7225 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5294 5326 6807 6839 5294 Size: 32 1481 32 5680 Fragments arranged by size: 5680 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7225 PsiI TTA'TAA Cuts at: 4795 4795 Size: 7225 PstI C_TGCA'G Cuts at: 830 3565 4577 830 Size: 2735 1012 3478 Fragments arranged by size: 3478 2735 1012 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7225 PvuII CAG'CTG Cuts at: 2397 3167 2397 Size: 770 6455 SacI G_AGCT'C Cuts at: 2113 3418 4412 2113 Size: 1305 994 4926 Fragments arranged by size: 4926 1305 994 SalI G'TCGA_C Cuts at: 1936 4567 1936 Size: 2631 4594 SbfI CC_TGCA'GG Cuts at: 4577 4577 Size: 7225 ScaI AGT'ACT Cuts at: 5473 7185 5473 Size: 1712 5513 SexAI A'CCwGG_T Cuts at: 3616 3616 Size: 7225 SfcI C'TryA_G Cuts at: 826 1045 2149 3217 3561 4507 4573 5708 6389 Size: 219 1104 1068 344 946 66 1135 681 Cuts at: 6389 6580 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1104 1068 946 681 344 219 191 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7225 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7225 SmaI CCC'GGG Cuts at: 1872 4552 1872 Size: 2680 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4174 4396 5329 6230 6492 820 Size: 197 926 2231 222 933 901 262 1553 Fragments arranged by size: 2231 1553 933 926 901 262 222 197 SnaBI TAC'GTA Cuts at: 493 4020 5016 493 Size: 3527 996 2702 Fragments arranged by size: 3527 2702 996 SpeI A'CTAG_T Cuts at: 152 152 Size: 7225 SphI G_CATG'C Cuts at: 1132 4583 1132 Size: 3451 3774 SspI AAT'ATT Cuts at: 5 52 2785 5149 5 Size: 47 2733 2364 2081 Fragments arranged by size: 2733 2364 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7225 StyI C'CwwG_G Cuts at: 513 1065 2436 3363 3865 4066 4306 4431 4513 Size: 552 1371 927 502 201 240 125 82 Cuts at: 4513 4664 513 Size: 151 3074 Fragments arranged by size: 3074 1371 927 552 502 240 201 151 125 82 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2900 5252 5269 5422 5607 6052 325 Size: 1255 1320 2352 17 153 185 445 1498 Fragments arranged by size: 2352 1498 1320 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5471 7183 96 Size: 274 80 33 51 1443 3494 1712 138 Fragments arranged by size: 3494 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2487 2519 7185 7217 2487 Size: 32 4666 32 2495 Fragments arranged by size: 4666 2495 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7225 XbaI T'CTAG_A Cuts at: 4561 4561 Size: 7225 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7225 XmaI C'CCGG_G Cuts at: 1870 4550 1870 Size: 2680 4545 XmnI GAAnn'nnTTC Cuts at: 2371 3493 4468 5354 2371 Size: 1122 975 886 4242 Fragments arranged by size: 4242 1122 975 886 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5033 276 Size: 53 83 186 936 3499 2468 Fragments arranged by size: 3499 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BstZ17I EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI