(Linear) (Six-base) MAPSORT of: KIEE4583.seq Check: 8119 from: 1 to: 2520 KIEE4583.orf With 182 enzymes: SgfI * March 22, 2012 18:25 .. AccI GT'mk_AC Cuts at: 0 1460 2520 Size: 1460 1060 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 2109 2402 2520 Size: 103 2006 293 118 Fragments arranged by size: 2006 293 118 103 AflIII A'CryG_T Cuts at: 0 84 2024 2520 Size: 84 1940 496 Fragments arranged by size: 1940 496 84 AleI CACnn'nnGTG Cuts at: 0 2171 2520 Size: 2171 349 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 404 436 2520 Size: 404 32 2084 Fragments arranged by size: 2084 404 32 AlwNI CAG_nnn'CTG Cuts at: 0 824 2109 2520 Size: 824 1285 411 Fragments arranged by size: 1285 824 411 ApoI r'AATT_y Cuts at: 0 235 514 572 918 993 2520 Size: 235 279 58 346 75 1527 Fragments arranged by size: 1527 346 279 235 75 58 BamHI G'GATC_C Cuts at: 0 1316 2520 Size: 1316 1204 BanI G'GyrC_C Cuts at: 0 1447 2520 Size: 1447 1073 BanII G_rGCy'C Cuts at: 0 108 889 1413 1426 1798 2010 2407 2478 Size: 108 781 524 13 372 212 397 71 Cuts at: 2478 2520 Size: 42 Fragments arranged by size: 781 524 397 372 212 108 71 42 13 BbsI GAAGACnn'nnnn_ Cuts at: 0 1481 1730 2267 2520 Size: 1481 249 537 253 Fragments arranged by size: 1481 537 253 249 BbvCI CC'TCA_GC Cuts at: 0 1158 2520 Size: 1158 1362 BclI T'GATC_A Cuts at: 0 32 2520 Size: 32 2488 BglI GCCn_nnn'nGGC Cuts at: 0 1223 2520 Size: 1223 1297 BglII A'GATC_T Cuts at: 0 561 2520 Size: 561 1959 BlpI GC'TnA_GC Cuts at: 0 2116 2520 Size: 2116 404 Bme1580I G_kGCm'C Cuts at: 0 1184 2493 2520 Size: 1184 1309 27 Fragments arranged by size: 1309 1184 27 BmgBI CAC'GTC Cuts at: 0 1092 2025 2499 2520 Size: 1092 933 474 21 Fragments arranged by size: 1092 933 474 21 BmrI ACTGGGnnnn_n' Cuts at: 0 577 1948 2520 Size: 577 1371 572 Fragments arranged by size: 1371 577 572 BmtI G_CTAG'C Cuts at: 0 670 2520 Size: 670 1850 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2497 2520 Size: 2497 23 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 1080 1937 2520 Size: 130 950 857 583 Fragments arranged by size: 950 857 583 130 Bpu10I CC'TnA_GC Cuts at: 0 1104 1158 1666 2520 Size: 1104 54 508 854 Fragments arranged by size: 1104 854 508 54 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2190 2376 2520 Size: 2190 186 144 Fragments arranged by size: 2190 186 144 BsaI GGTCTCn'nnnn_ Cuts at: 0 2126 2287 2520 Size: 2126 161 233 Fragments arranged by size: 2126 233 161 BsaAI yAC'GTr Cuts at: 0 87 1965 2015 2520 Size: 87 1878 50 505 Fragments arranged by size: 1878 505 87 50 BsaBI GATnn'nnATC Cuts at: 0 176 2520 Size: 176 2344 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 482 512 2520 Size: 482 30 2008 Fragments arranged by size: 2008 482 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 506 989 2520 Size: 506 483 1531 Fragments arranged by size: 1531 506 483 BseYI C'CCAG_C Cuts at: 0 1145 1660 1821 2520 Size: 1145 515 161 699 Fragments arranged by size: 1145 699 515 161 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2149 2520 Size: 2149 371 BsiEI CG_ry'CG Cuts at: 0 2268 2520 Size: 2268 252 BsiHKAI G_wGCw'C Cuts at: 0 108 259 1413 2407 2520 Size: 108 151 1154 994 113 Fragments arranged by size: 1154 994 151 113 108 BsiWI C'GTAC_G Cuts at: 0 2015 2520 Size: 2015 505 BsmI GAATG_Cn' Cuts at: 0 1815 2038 2520 Size: 1815 223 482 Fragments arranged by size: 1815 482 223 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1098 2520 Size: 1098 1422 Bsp1286I G_dGCh'C Cuts at: 0 108 259 889 1184 1413 1426 1798 2010 Size: 108 151 630 295 229 13 372 212 Cuts at: 2010 2407 2478 2493 2520 Size: 397 71 15 27 Fragments arranged by size: 630 397 372 295 229 212 151 108 71 27 15 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 839 2520 Size: 839 1681 BsrDI GCAATG_nn' Cuts at: 0 851 1157 1269 1934 2447 2520 Size: 851 306 112 665 513 73 Fragments arranged by size: 851 665 513 306 112 73 BssSI C'ACGA_G Cuts at: 0 1192 2520 Size: 1192 1328 BstAPI GCAn_nnn'nTGC Cuts at: 0 2109 2520 Size: 2109 411 BstEII G'GTnAC_C Cuts at: 0 1186 2520 Size: 1186 1334 BstXI CCAn_nnnn'nTGG Cuts at: 0 1821 2520 Size: 1821 699 BstYI r'GATC_y Cuts at: 0 561 1316 2520 Size: 561 755 1204 Fragments arranged by size: 1204 755 561 Bsu36I CC'TnA_GG Cuts at: 0 424 1338 2221 2520 Size: 424 914 883 299 Fragments arranged by size: 914 883 424 299 BtgI C'CryG_G Cuts at: 0 431 1403 2520 Size: 431 972 1117 Fragments arranged by size: 1117 972 431 DraIII CAC_nnn'GTG Cuts at: 0 70 1615 2520 Size: 70 1545 905 Fragments arranged by size: 1545 905 70 EaeI y'GGCC_r Cuts at: 0 864 1224 1400 1893 1959 2265 2520 Size: 864 360 176 493 66 306 255 Fragments arranged by size: 864 493 360 306 255 176 66 EagI C'GGCC_G Cuts at: 0 2265 2520 Size: 2265 255 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2081 2520 Size: 2081 439 EcoICRI GAG'CTC Cuts at: 0 106 1411 2405 2520 Size: 106 1305 994 115 Fragments arranged by size: 1305 994 115 106 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 130 1080 1937 2109 2402 2520 Size: 103 27 950 857 172 293 118 Fragments arranged by size: 950 857 293 172 118 103 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 1171 1300 2520 Size: 1171 129 1220 Fragments arranged by size: 1220 1171 129 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 439 2483 2520 Size: 439 2044 37 Fragments arranged by size: 2044 439 37 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1000 1032 2011 2043 2224 2256 2520 Size: 1000 32 979 32 181 32 264 Fragments arranged by size: 1000 979 264 181 32 32 32 HincII GTy'rAC Cuts at: 0 141 948 1287 2520 Size: 141 807 339 1233 Fragments arranged by size: 1233 807 339 141 HindIII A'AGCT_T Cuts at: 0 795 2520 Size: 795 1725 HpaI GTT'AAC Cuts at: 0 141 2520 Size: 141 2379 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 785 1349 1983 2520 Size: 785 564 634 537 Fragments arranged by size: 785 634 564 537 MscI TGG'CCA Cuts at: 0 1961 2520 Size: 1961 559 MslI CAynn'nnrTG Cuts at: 0 170 2077 2171 2416 2520 Size: 170 1907 94 245 104 Fragments arranged by size: 1907 245 170 104 94 MspA1I CmG'CkG Cuts at: 0 392 1162 2129 2520 Size: 392 770 967 391 Fragments arranged by size: 967 770 392 391 NcoI C'CATG_G Cuts at: 0 431 2520 Size: 431 2089 NheI G'CTAG_C Cuts at: 0 666 2520 Size: 666 1854 NspI r_CATG'y Cuts at: 0 860 2082 2520 Size: 860 1222 438 Fragments arranged by size: 1222 860 438 PasI CC'CwG_GG Cuts at: 0 320 882 2520 Size: 320 562 1638 Fragments arranged by size: 1638 562 320 PfoI T'CCnGG_A Cuts at: 0 997 2520 Size: 997 1523 PstI C_TGCA'G Cuts at: 0 1560 2520 Size: 1560 960 PvuII CAG'CTG Cuts at: 0 392 1162 2520 Size: 392 770 1358 Fragments arranged by size: 1358 770 392 SacI G_AGCT'C Cuts at: 0 108 1413 2407 2520 Size: 108 1305 994 113 Fragments arranged by size: 1305 994 113 108 SexAI A'CCwGG_T Cuts at: 0 1611 2520 Size: 1611 909 SfcI C'TryA_G Cuts at: 0 144 1212 1556 2502 2520 Size: 144 1068 344 946 18 Fragments arranged by size: 1068 946 344 144 18 SmlI C'TyrA_G Cuts at: 0 2169 2391 2520 Size: 2169 222 129 Fragments arranged by size: 2169 222 129 SnaBI TAC'GTA Cuts at: 0 2015 2520 Size: 2015 505 SspI AAT'ATT Cuts at: 0 780 2520 Size: 780 1740 StyI C'CwwG_G Cuts at: 0 431 1358 1860 2061 2301 2426 2508 2520 Size: 431 927 502 201 240 125 82 12 Fragments arranged by size: 927 502 431 240 201 125 82 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 895 2520 Size: 895 1625 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 482 514 2520 Size: 482 32 2006 Fragments arranged by size: 2006 482 32 XmnI GAAnn'nnTTC Cuts at: 0 366 1488 2463 2520 Size: 366 1122 975 57 Fragments arranged by size: 1122 975 366 57 Enzymes that do cut: AccI AcuI AflIII AleI AloI AlwNI ApoI BamHI BanI BanII BbsI BbvCI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspMI BsrDI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI DraIII EaeI EagI EciI EcoICRI Eco57MI EcoNI FalI Hin4I HincII HindIII HpaI MmeI MscI MslI MspA1I NcoI NheI NspI PasI PfoI PstI PvuII SacI SexAI SfcI SmlI SnaBI SspI StyI TaqII TstI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI BcgI BciVI BfrBI BsaHI BsaWI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstBI BstZ17I BtgZI BtsI ClaI CspCI DraI DrdI EarI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI StuI SwaI TatI Tth111I XbaI XcmI XhoI XmaI ZraI