################################## # NO CUT ENZYMES ( 201 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI ItaI KasI KpnI KspI MaeI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI TseI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AluI 140 AGCT AG^CT AlwI 562 GATCC GGATC(4/5) AseI 472 ATTAAT AT^TAAT AsnI 472 ATTAAT AT^TAAT AvaII 580 GGTCC G^G[AT]CC BcnI 243 CCGGG CC^[GC]GG BseRI 455 GAGGAG GAGGAG(10/8) BsiYI 202 CCTCTTTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 202 CCTCTTTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 246 GGGAC GGGAC(10/14) BstAPI 460 GCATTAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr13I 580 GGTCC G^G[ACGT]CC EarI 31 GAAGAG CTCTTC(1/4) HapII 243 CCGG C^CGG HinfI 309 GAATC G^A[ACGT]TC HpaII 243 CCGG C^CGG Ksp632I 31 GAAGAG CTCTTC(1/4) MaeII 642 ACGT A^CGT MspI 243 CCGG C^CGG MwoI 460 GCATTAGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 243 CCGGG CC^[GC]GG NlaIV 349 GGAACC GG[ACGT]^[ACGT]CC NspI 600 ACATGC [GA]CATG^[CT] PshBI 472 ATTAAT AT^TAAT Sau96I 580 GGTCC G^G[ACGT]CC SfaNI 661 GATGC GCATC(5/9) SmlI 649 CTTGAG C^T[CT][GA]AG SspI 424 AATATT AAT^ATT TfiI 309 GAATC G^A[AT]TC ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### AciI 209 GCGG CCGC(-3/-1) 217 GCGG BciVI 42 GGATAC GTATCC(6/5) 284 GGATAC BstF5I 574 CATCC GGATG(2/0) 3 GGATG FokI 574 CATCC GGATG(9/13) 3 GGATG NlaIII 441 CATG CATG^ 601 CATG ScaI 35 AGTACT AGT^ACT 66 AGTACT TspRI 252 CAGTG CA[GC]TG[ACGT][ACGT]^ 273 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 21 enzymes) # ######################################### AcsI AfaI ApoI BsaJI BssKI BstNI DdeI DpnII EcoRII HphI MboI MboII MnlI MseI MvaI NdeII RsaI Sau3AI ScrFI Tru9I Tsp509I