################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BanII BbrPI BbsI BbvCI BclI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Cac8I CelII Cfr10I ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI Ksp632I MaeII MamI MfeI MluI MluNI MroI MscI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI RcaI RsrII SacI SapI SbfI SexAI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp45I Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 445 GTCGAC GT^[AC][GT]AC AcyI 591 GGCGCC G[GA]^CG[CT]C AocI 307 CCTCAGG CC^T[ACGT]AGG BamHI 688 GGATCC G^GATCC BanI 591 GGCGCC G^G[CT][GA]CC BbeI 591 GGCGCC GGCGC^C BbvI 516 GCTGC GCAGC(8/12) BcgI 213 CGAGTCCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 111 GGATAC GTATCC(6/5) BsaHI 591 GGCGCC G[GA]^CG[CT]C BsgI 764 GTGCAG GTGCAG(16/14) BsiEI 818 CGATCG CG[GA][CT]^CG BssSI 211 CACGAG CACGAG(-5/-1) BstNI 801 CCAGG CC^[AT]GG Bsu36I 307 CCTCAGG CC^T[ACGT]AGG CfoI 592 GCGC GCG^C DraI 736 TTTAAA TTT^AAA DraII 859 GGGACCC [GA]G^G[ACGT]CC[CT] Eco81I 307 CCTCAGG CC^T[ACGT]AGG EcoO109I 859 GGGACCC [GA]G^G[ACGT]CC[CT] EcoRII 801 CCAGG ^CC[AT]GG FauI 634 GCGGG CCCGC(4/6) HaeII 591 GGCGCC [GA]GCGC^[CT] HaeIII 831 GGCC GG^CC HhaI 592 GCGC GCG^C Hin1I 591 GGCGCC G[GA]^CG[CT]C HinP1I 592 GCGC G^CGC HincII 445 GTCGAC GT[CT]^[GA]AC HindII 445 GTCGAC GT[CT]^[GA]AC KasI 591 GGCGCC G^GCGCC KspI 587 CCGCGG CCGC^GG MslI 170 CATTGGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 801 CCAGG CC^[AT]GG MwoI 510 GCCCCAGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NarI 591 GGCGCC GG^CGCC NcoI 419 CCATGG C^CATGG NspI 329 GCATGT [GA]CATG^[CT] PpuMI 859 GGGACCC [GA]G^G[AT]CC[CT] PshAI 861 GACCCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 818 CGATCG CGAT^CG SacII 587 CCGCGG CCGC^GG SalI 445 GTCGAC G^TCGAC ScaI 251 AGTACT AGT^ACT SfaNI 298 GATGC GCATC(5/9) SfoI 591 GGCGCC GGC^GCC SspI 793 AATATT AAT^ATT TseI 516 GCTGC G^C[AT]GC XcmI 419 CCATGGAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 588 CGCG CG^CG 816 CGCG AcsI 86 AAATTC [GA]^AATT[CT] 722 AAATTT ApoI 86 AAATTC [GA]^AATT[CT] 722 AAATTT Asp700I 394 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 790 GAAAATATTC AvaII 860 GGACC G^G[AT]CC 867 GGTCC BcnI 498 CCGGG CC^[GC]GG 864 CCCGG BfaI 190 CTAG C^TAG 703 CTAG BpmI 340 CTGGAG CTGGAG(16/14) 379 CTGGAG BstUI 588 CGCG CG^CG 816 CGCG BstYI 688 GGATCC [GA]^GATC[CT] 698 AGATCC Cfr13I 860 GGACC G^G[ACGT]CC 867 GGTCC DsaI 419 CCATGG C^C[GA][CT]GG 587 CCGCGG EcoT14I 224 CCAAGG C^C[AT][AT]GG 419 CCATGG Fnu4HI 516 GCTGC GC^[ACGT]GC 589 GCGGC HapII 498 CCGG C^CGG 865 CCGG HgaI 467 GACGC GACGC(5/10) 814 GACGC HpaII 498 CCGG C^CGG 865 CCGG HphI 228 GGTGA GGTGA(8/7) 461 TCACC ItaI 516 GCTGC GC^[ACGT]GC 589 GCGGC MaeI 190 CTAG C^TAG 703 CTAG MaeIII 354 GTTAC ^GT[ACGT]AC 452 GTTAC MflI 688 GGATCC [GA]^GATC[CT] 698 AGATCC MspI 498 CCGG C^CGG 865 CCGG MvnI 588 CGCG CG^CG 816 CGCG NciI 498 CCGGG CC^[GC]GG 864 CCCGG PvuII 337 CAGCTG CAG^CTG 514 CAGCTG Sau96I 860 GGACC G^G[ACGT]CC 867 GGTCC StyI 224 CCAAGG C^C[AT][AT]GG 419 CCATGG XhoII 688 GGATCC [GA]^GATC[CT] 698 AGATCC XmnI 394 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 790 GAAAATATTC ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AciI AfaI AluI AlwI BsaJI BsiYI BslI BsmFI BsrI BssKI BstF5I DdeI DpnII FokI HinfI MboI MboII MnlI MseI MspA1I NdeII NlaIII NlaIV PleI RsaI Sau3AI ScrFI TaqI TfiI Tru9I Tsp509I TspRI TthHB8I