(Circular) (Six-base) MAPSORT of: pFN21AE2217.seq Check: 172 from: 1 to: 5584 pFN21AE2217 5584 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3394 278 Size: 53 83 186 936 1858 2468 Fragments arranged by size: 2468 1858 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2451 2927 1937 Size: 514 476 4594 Fragments arranged by size: 4594 514 476 Acc65I G'GTAC_C Cuts at: 2905 2905 Size: 5584 AclI AA'CG_TT Cuts at: 1563 3711 4084 1563 Size: 2148 373 3063 Fragments arranged by size: 3063 2148 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2340 3647 5031 5299 1632 Size: 708 1307 1384 268 1917 Fragments arranged by size: 1917 1384 1307 708 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5387 AflIII A'CryG_T Cuts at: 1203 4483 5460 1203 Size: 3280 977 1327 Fragments arranged by size: 3280 1327 977 AgeI A'CCGG_T Cuts at: 4415 4415 Size: 5584 AhdI GACnn_n'nnGTC Cuts at: 4313 4313 Size: 5584 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5584 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5584 AlwNI CAG_nnn'CTG Cuts at: 3327 4899 3327 Size: 1572 4012 ApaLI G'TGCA_C Cuts at: 3641 4797 3641 Size: 1156 4428 ApoI r'AATT_y Cuts at: 1493 2091 2727 2893 3119 3271 5380 1493 Size: 598 636 166 226 152 2109 1697 Fragments arranged by size: 2109 1697 636 598 226 166 152 AseI AT'TA_AT Cuts at: 160 1032 4138 160 Size: 872 3106 1606 Fragments arranged by size: 3106 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5584 AvaI C'yCGr_G Cuts at: 1870 1943 2909 1870 Size: 73 966 4545 Fragments arranged by size: 4545 966 73 BamHI G'GATC_C Cuts at: 2693 2914 3281 2693 Size: 221 367 4996 Fragments arranged by size: 4996 367 221 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2596 2905 4360 618 Size: 325 217 627 809 309 1455 1842 Fragments arranged by size: 1842 1455 809 627 325 309 217 BanII G_rGCy'C Cuts at: 1391 2903 1391 Size: 1512 4072 BbeI G_GCGC'C Cuts at: 2600 2600 Size: 5584 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4613 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2207 2241 3775 3809 1903 Size: 34 270 34 1534 34 3678 Fragments arranged by size: 3678 1534 270 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2110 3476 4686 2110 Size: 1366 1210 3008 Fragments arranged by size: 3008 1366 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5584 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4195 136 Size: 107 122 71 631 605 2523 1525 Fragments arranged by size: 2523 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5579 5579 Size: 5584 BlpI GC'TnA_GC Cuts at: 3001 3001 Size: 5584 Bme1580I G_kGCm'C Cuts at: 1790 3645 4801 1790 Size: 1855 1156 2573 Fragments arranged by size: 2573 1855 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5584 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3315 4273 442 Size: 416 53 83 399 24 1898 958 1753 Fragments arranged by size: 1898 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5584 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2169 2169 Size: 5584 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2366 2405 4244 1239 Size: 1127 39 1839 2579 Fragments arranged by size: 2579 1839 1127 39 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3709 4574 4872 3709 Size: 865 298 4421 Fragments arranged by size: 4421 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4247 882 Size: 617 2748 2219 Fragments arranged by size: 2748 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3375 493 Size: 711 2171 2702 Fragments arranged by size: 2702 2171 711 BsaBI GATnn'nnATC Cuts at: 5578 5578 Size: 5584 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2597 3391 3773 4408 Size: 53 83 186 936 1064 794 382 635 Cuts at: 4408 275 Size: 1451 Fragments arranged by size: 1451 1064 936 794 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4017 4415 4689 4836 5247 4017 Size: 398 274 147 411 4354 Fragments arranged by size: 4354 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5359 Fragments arranged by size: 5359 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5584 BseYI C'CCAG_C Cuts at: 2517 4787 5283 2517 Size: 2270 496 2818 Fragments arranged by size: 2818 2270 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2790 1163 Size: 573 1054 3957 Fragments arranged by size: 3957 1054 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2826 3034 3795 4823 1540 Size: 275 186 825 208 761 1028 2301 Fragments arranged by size: 2301 1028 825 761 275 208 186 BsiHKAI G_wGCw'C Cuts at: 3645 3730 4801 3645 Size: 85 1071 4428 Fragments arranged by size: 4428 1071 85 BsmI GAATG_Cn' Cuts at: 3095 3188 3095 Size: 93 5491 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2903 3645 3730 4801 1391 Size: 399 1113 742 85 1071 2174 Fragments arranged by size: 2174 1113 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5247 5247 Size: 5584 BspHI T'CATG_A Cuts at: 3473 5203 3473 Size: 1730 3854 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2939 844 Size: 2095 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3471 1394 Size: 429 1648 3507 Fragments arranged by size: 3507 1648 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4079 4253 65 Size: 1183 2831 174 1396 Fragments arranged by size: 2831 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4228 4415 1700 Size: 110 2418 187 2869 Fragments arranged by size: 2869 2418 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5584 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5584 BssSI C'ACGA_G Cuts at: 2216 3644 4656 2216 Size: 1428 1012 3144 Fragments arranged by size: 3144 1428 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5584 BstBI TT'CG_AA Cuts at: 4388 4388 Size: 5584 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5584 BstYI r'GATC_y Cuts at: 1972 2693 2703 2914 3281 3666 3683 5135 5221 Size: 721 10 211 367 385 17 1452 86 Cuts at: 5221 5233 5579 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 721 385 367 346 211 86 17 12 10 Bsu36I CC'TnA_GG Cuts at: 2313 2313 Size: 5584 BtgI C'CryG_G Cuts at: 513 1065 2424 2592 513 Size: 552 1359 168 3505 Fragments arranged by size: 3505 1359 552 168 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4845 Fragments arranged by size: 4845 683 56 BtsI GCAGTG_nn' Cuts at: 3100 3894 3914 3100 Size: 794 20 4770 Fragments arranged by size: 4770 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5584 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2842 2877 529 Size: 35 2278 35 3236 Fragments arranged by size: 3236 2278 35 35 DraI TTT'AAA Cuts at: 2743 2888 2743 Size: 145 5439 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4591 809 Size: 507 3275 1802 Fragments arranged by size: 3275 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3031 3920 5549 Size: 54 1425 50 275 18 1201 889 1629 Cuts at: 5549 8 Size: 43 Fragments arranged by size: 1629 1425 1201 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3031 1537 Size: 275 1219 4090 Fragments arranged by size: 4090 1219 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3514 1353 Size: 2161 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4171 4545 4691 387 Size: 1064 138 2582 374 146 1280 Fragments arranged by size: 2582 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2340 2366 2405 3647 4244 5031 5299 Size: 393 708 26 39 1242 597 787 268 Cuts at: 5299 1239 Size: 1524 Fragments arranged by size: 1524 1242 787 708 597 393 268 39 26 EcoO109I rG'GnC_Cy Cuts at: 2865 2865 Size: 5584 EcoRI G'AATT_C Cuts at: 2893 3271 2893 Size: 378 5206 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2391 2423 2650 2391 Size: 32 227 5325 Fragments arranged by size: 5325 227 32 FspI TGC'GCA Cuts at: 4090 4090 Size: 5584 HaeII r_GCGC'y Cuts at: 1678 2600 4731 1678 Size: 922 2131 2531 Fragments arranged by size: 2531 2131 922 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2227 2259 4225 4257 4299 Size: 32 135 32 655 32 1966 32 42 Cuts at: 4299 4331 1373 Size: 32 2626 Fragments arranged by size: 2626 1966 655 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2452 2928 3174 669 Size: 988 281 514 476 246 3079 Fragments arranged by size: 3079 988 514 476 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5584 HpaI GTT'AAC Cuts at: 3174 3174 Size: 5584 KasI G'GCGC_C Cuts at: 2596 2596 Size: 5584 KpnI G_GTAC'C Cuts at: 2909 2909 Size: 5584 MfeI C'AATT_G Cuts at: 3183 3183 Size: 5584 MluI A'CGCG_T Cuts at: 5460 5460 Size: 5584 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4698 4882 1305 Size: 3393 184 2007 Fragments arranged by size: 3393 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3762 Fragments arranged by size: 3762 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2179 3542 3901 4060 518 Size: 648 1013 1363 359 159 2042 Fragments arranged by size: 2042 1363 1013 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2344 2521 2594 3000 3677 4825 5070 1606 Size: 738 177 73 406 677 1148 245 2120 Fragments arranged by size: 2120 1148 738 677 406 245 177 73 NarI GG'CG_CC Cuts at: 2597 2597 Size: 5584 NcoI C'CATG_G Cuts at: 513 1065 2424 513 Size: 552 1359 3673 Fragments arranged by size: 3673 1359 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5584 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5584 NotI GC'GGCC_GC Cuts at: 3031 3031 Size: 5584 NspI r_CATG'y Cuts at: 1132 1229 2338 2942 4487 1132 Size: 97 1109 604 1545 2229 Fragments arranged by size: 2229 1545 1109 604 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5584 PciI A'CATG_T Cuts at: 4483 4483 Size: 5584 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5584 PmeI GTTT'AAAC Cuts at: 2888 2888 Size: 5584 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2572 2604 3653 3685 5166 5198 2572 Size: 32 1049 32 1481 32 2958 Fragments arranged by size: 2958 1481 1049 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2865 2865 Size: 5584 PshAI GACnn'nnGTC Cuts at: 1613 2870 1613 Size: 1257 4327 PsiI TTA'TAA Cuts at: 3154 3154 Size: 5584 PstI C_TGCA'G Cuts at: 830 2936 830 Size: 2106 3478 PvuI CG_AT'CG Cuts at: 2001 2826 2001 Size: 825 4759 PvuII CAG'CTG Cuts at: 2344 2521 2344 Size: 177 5407 SacII CC_GC'GG Cuts at: 2595 2595 Size: 5584 SalI G'TCGA_C Cuts at: 1936 2450 2926 1936 Size: 514 476 4594 Fragments arranged by size: 4594 514 476 SanDI GG'GwC_CC Cuts at: 2865 2865 Size: 5584 SbfI CC_TGCA'GG Cuts at: 2936 2936 Size: 5584 ScaI AGT'ACT Cuts at: 2258 3832 5544 2258 Size: 1574 1712 2298 Fragments arranged by size: 2298 1712 1574 SfcI C'TryA_G Cuts at: 826 1045 2932 4067 4748 4939 826 Size: 219 1887 1135 681 191 1471 Fragments arranged by size: 1887 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2598 2598 Size: 5584 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5584 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5584 SmaI CCC'GGG Cuts at: 1872 2911 1872 Size: 1039 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3688 4589 4851 820 Size: 197 926 1745 901 262 1553 Fragments arranged by size: 1745 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3375 493 Size: 2882 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5584 SphI G_CATG'C Cuts at: 1132 2942 1132 Size: 1810 3774 SspI AAT'ATT Cuts at: 5 52 2800 3508 5 Size: 47 2748 708 2081 Fragments arranged by size: 2748 2081 708 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5584 StyI C'CwwG_G Cuts at: 513 1065 2229 2424 3023 513 Size: 552 1164 195 599 3074 Fragments arranged by size: 3074 1164 599 552 195 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3611 3628 3781 3966 4411 325 Size: 1255 2031 17 153 185 445 1498 Fragments arranged by size: 2031 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2132 2256 3830 Size: 274 80 33 51 1443 155 124 1574 Cuts at: 3830 5542 96 Size: 1712 138 Fragments arranged by size: 1712 1574 1443 274 155 138 124 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2204 2236 5544 5576 2204 Size: 32 3308 32 2212 Fragments arranged by size: 3308 2212 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5584 XbaI T'CTAG_A Cuts at: 2920 2920 Size: 5584 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2431 2431 Size: 5584 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5584 XmaI C'CCGG_G Cuts at: 1870 2909 1870 Size: 1039 4545 XmnI GAAnn'nnTTC Cuts at: 2403 2799 3713 2403 Size: 396 914 4274 Fragments arranged by size: 4274 914 396 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3392 276 Size: 53 83 186 936 1858 2468 Fragments arranged by size: 2468 1858 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SapI SexAI SfiI SrfI SwaI