(Circular) (Six-base) MAPSORT of: pFN21AE1955.seq Check: 7958 from: 1 to: 6244 pFN21AE1955 6244 bp With 182 enzymes: SgfI * March 30, 2011 18:12 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4054 278 Size: 53 83 186 936 2518 2468 Fragments arranged by size: 2518 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3587 1937 Size: 1650 4594 Acc65I G'GTAC_C Cuts at: 3565 3565 Size: 6244 AclI AA'CG_TT Cuts at: 1563 4371 4744 1563 Size: 2808 373 3063 Fragments arranged by size: 3063 2808 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2411 2969 3189 4307 5691 5959 1632 Size: 779 558 220 1118 1384 268 1917 Fragments arranged by size: 1917 1384 1118 779 558 268 220 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6047 AflIII A'CryG_T Cuts at: 1203 5143 6120 1203 Size: 3940 977 1327 Fragments arranged by size: 3940 1327 977 AgeI A'CCGG_T Cuts at: 5075 5075 Size: 6244 AhdI GACnn_n'nnGTC Cuts at: 2458 4973 2458 Size: 2515 3729 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6244 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6244 AlwNI CAG_nnn'CTG Cuts at: 2035 2067 2134 2359 3987 5559 2035 Size: 32 67 225 1628 1572 2720 Fragments arranged by size: 2720 1628 1572 225 67 32 ApaI G_GGCC'C Cuts at: 2076 2076 Size: 6244 ApaLI G'TGCA_C Cuts at: 3107 4301 5457 3107 Size: 1194 1156 3894 Fragments arranged by size: 3894 1194 1156 ApoI r'AATT_y Cuts at: 1493 2768 2924 3553 3779 3931 6040 1493 Size: 1275 156 629 226 152 2109 1697 Fragments arranged by size: 2109 1697 1275 629 226 156 152 AseI AT'TA_AT Cuts at: 160 1032 4798 160 Size: 872 3766 1606 Fragments arranged by size: 3766 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6244 AvaI C'yCGr_G Cuts at: 1870 1943 2194 2381 2530 3027 3413 3569 1870 Size: 73 251 187 149 497 386 156 4545 Fragments arranged by size: 4545 497 386 251 187 156 149 73 BamHI G'GATC_C Cuts at: 3574 3941 3574 Size: 367 5877 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2534 3141 3565 5020 618 Size: 325 217 627 747 607 424 1455 1842 Fragments arranged by size: 1842 1455 747 627 607 424 325 217 BanII G_rGCy'C Cuts at: 1391 2076 2153 2271 2376 3563 1391 Size: 685 77 118 105 1187 4072 Fragments arranged by size: 4072 1187 685 118 105 77 BbeI G_GCGC'C Cuts at: 2538 3145 2538 Size: 607 5637 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5273 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2345 2379 2487 2521 4435 Size: 34 50 34 324 34 108 34 1914 Cuts at: 4435 4469 1903 Size: 34 3678 Fragments arranged by size: 3678 1914 324 108 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4136 5346 4136 Size: 1210 5034 BclI T'GATC_A Cuts at: 1548 1548 Size: 6244 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3045 4855 136 Size: 107 122 71 631 605 1373 1810 1525 Fragments arranged by size: 1810 1525 1373 631 605 122 107 71 BglII A'GATC_T Cuts at: 2250 6239 2250 Size: 3989 2255 BlpI GC'TnA_GC Cuts at: 2990 3661 2990 Size: 671 5573 Bme1580I G_kGCm'C Cuts at: 1790 2076 3111 4305 5461 1790 Size: 286 1035 1194 1156 2573 Fragments arranged by size: 2573 1194 1156 1035 286 BmgBI CAC'GTC Cuts at: 1318 3019 3184 1318 Size: 1701 165 4378 Fragments arranged by size: 4378 1701 165 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3166 3975 4933 Size: 416 53 83 399 24 1749 809 958 Cuts at: 4933 442 Size: 1753 Fragments arranged by size: 1753 1749 958 809 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6244 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3118 3181 4904 1239 Size: 1879 63 1723 2579 Fragments arranged by size: 2579 1879 1723 63 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2201 4369 5234 5532 2201 Size: 2168 865 298 2913 Fragments arranged by size: 2913 2168 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3199 4907 882 Size: 617 1700 1708 2219 Fragments arranged by size: 2219 1708 1700 617 BsaAI yAC'GTr Cuts at: 493 1204 3112 4035 493 Size: 711 1908 923 2702 Fragments arranged by size: 2702 1908 923 711 BsaBI GATnn'nnATC Cuts at: 6238 6238 Size: 6244 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2459 2535 3142 4051 Size: 53 83 186 936 926 76 607 909 Cuts at: 4051 4433 5068 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 926 909 635 607 382 186 83 76 53 BsaWI w'CCGG_w Cuts at: 4677 5075 5349 5496 5907 4677 Size: 398 274 147 411 5014 Fragments arranged by size: 5014 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2240 2270 3003 3033 1178 Size: 30 165 30 837 30 733 30 4389 Fragments arranged by size: 4389 837 733 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2182 2351 1186 Size: 996 169 5079 Fragments arranged by size: 5079 996 169 BseYI C'CCAG_C Cuts at: 2014 2134 2263 2511 5447 5943 2014 Size: 120 129 248 2936 496 2315 Fragments arranged by size: 2936 2315 496 248 129 120 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2080 2276 3407 1163 Size: 573 344 196 1131 4000 Fragments arranged by size: 4000 1131 573 344 196 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2112 2158 3694 4455 5483 1540 Size: 275 186 111 46 1536 761 1028 2301 Fragments arranged by size: 2301 1536 1028 761 275 186 111 46 BsiHKAI G_wGCw'C Cuts at: 3111 4305 4390 5461 3111 Size: 1194 85 1071 3894 Fragments arranged by size: 3894 1194 1071 85 BsmI GAATG_Cn' Cuts at: 3341 3370 3755 3848 3341 Size: 29 385 93 5737 Fragments arranged by size: 5737 385 93 29 BsmBI CGTCTCn'nnnn_ Cuts at: 3045 3045 Size: 6244 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2153 2271 2376 3111 3563 4305 Size: 399 286 77 118 105 735 452 742 Cuts at: 4305 4390 5461 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 735 452 399 286 118 105 85 77 BspEI T'CCGG_A Cuts at: 5907 5907 Size: 6244 BspHI T'CATG_A Cuts at: 2649 4133 5863 2649 Size: 1484 1730 3030 Fragments arranged by size: 3030 1730 1484 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3599 844 Size: 2755 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2548 4131 1394 Size: 429 725 1583 3507 Fragments arranged by size: 3507 1583 725 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3294 4739 4913 65 Size: 1183 2046 1445 174 1396 Fragments arranged by size: 2046 1445 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2208 2277 3138 4888 5075 1700 Size: 110 398 69 861 1750 187 2869 Fragments arranged by size: 2869 1750 861 398 187 110 69 BsrGI T'GTAC_A Cuts at: 96 2421 96 Size: 2325 3919 BssHII G'CGCG_C Cuts at: 1925 2610 1925 Size: 685 5559 BssSI C'ACGA_G Cuts at: 4304 5316 4304 Size: 1012 5232 BstAPI GCAn_nnn'nTGC Cuts at: 794 2067 2359 794 Size: 1273 292 4679 Fragments arranged by size: 4679 1273 292 BstBI TT'CG_AA Cuts at: 5048 5048 Size: 6244 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6244 BstYI r'GATC_y Cuts at: 1972 2250 2416 2576 3574 3941 4326 4343 5795 Size: 278 166 160 998 367 385 17 1452 Cuts at: 5795 5881 5893 6239 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 998 385 367 346 278 166 160 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2742 2742 Size: 6244 BtgI C'CryG_G Cuts at: 513 1065 2106 513 Size: 552 1041 4651 Fragments arranged by size: 4651 1041 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5505 Fragments arranged by size: 5505 683 56 BtsI GCAGTG_nn' Cuts at: 2036 3226 3760 4554 4574 2036 Size: 1190 534 794 20 3706 Fragments arranged by size: 3706 1190 794 534 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6244 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6209 DraI TTT'AAA Cuts at: 2806 3548 2806 Size: 742 5502 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3153 5251 809 Size: 507 1837 2098 1802 Fragments arranged by size: 2098 1837 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2109 2155 2937 Size: 54 1425 50 275 18 279 46 782 Cuts at: 2937 3691 4580 6209 8 Size: 754 889 1629 43 Fragments arranged by size: 1629 1425 889 782 754 279 275 54 50 46 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2109 2155 3691 1537 Size: 275 297 46 1536 4090 Fragments arranged by size: 4090 1536 297 275 46 EarI CTCTTCn'nnn_ Cuts at: 1353 2411 4174 1353 Size: 1058 1763 3423 Fragments arranged by size: 3423 1763 1058 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2035 2425 4831 5205 5351 387 Size: 1064 138 446 390 2406 374 146 1280 Fragments arranged by size: 2406 1280 1064 446 390 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2411 2969 3118 3181 3189 4307 4904 Size: 393 779 558 149 63 8 1118 597 Cuts at: 4904 5691 5959 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1118 787 779 597 558 393 268 149 63 8 EcoO109I rG'GnC_Cy Cuts at: 2073 2073 Size: 6244 EcoRI G'AATT_C Cuts at: 2768 3553 3931 2768 Size: 785 378 5081 Fragments arranged by size: 5081 785 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2269 2743 3091 2269 Size: 474 348 5422 Fragments arranged by size: 5422 474 348 FspI TGC'GCA Cuts at: 3116 4750 3116 Size: 1634 4610 FspAI rTGC'GCAy Cuts at: 3116 3116 Size: 6244 HaeII r_GCGC'y Cuts at: 1678 2324 2538 2625 3145 5391 1678 Size: 646 214 87 520 2246 2531 Fragments arranged by size: 2531 2246 646 520 214 87 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2240 2272 2444 2476 3003 Size: 32 135 32 668 32 172 32 527 Cuts at: 3003 3035 3145 3177 3470 3502 4885 4917 4959 Size: 32 110 32 293 32 1383 32 42 Cuts at: 4959 4991 1373 Size: 32 2626 Fragments arranged by size: 2626 1383 668 527 293 172 135 110 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3588 3834 669 Size: 988 281 1650 246 3079 Fragments arranged by size: 3079 1650 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6244 HpaI GTT'AAC Cuts at: 3834 3834 Size: 6244 KasI G'GCGC_C Cuts at: 2534 3141 2534 Size: 607 5637 KpnI G_GTAC'C Cuts at: 3569 3569 Size: 6244 MfeI C'AATT_G Cuts at: 3843 3843 Size: 6244 MluI A'CGCG_T Cuts at: 6120 6120 Size: 6244 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2326 2442 2862 5358 5542 1305 Size: 1021 116 420 2496 184 2007 Fragments arranged by size: 2496 2007 1021 420 184 116 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4422 Fragments arranged by size: 4422 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2648 3363 4202 4561 4720 518 Size: 648 1482 715 839 359 159 2042 Fragments arranged by size: 2042 1482 839 715 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2064 2083 2102 2108 2131 2359 2586 2989 Size: 458 19 19 6 23 228 227 403 Cuts at: 2989 3660 4337 5485 5730 1606 Size: 671 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 671 458 403 245 228 227 23 19 19 6 NaeI GCC'GGC Cuts at: 3140 3140 Size: 6244 NarI GG'CG_CC Cuts at: 2535 3142 2535 Size: 607 5637 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5692 NdeI CA'TA_TG Cuts at: 387 387 Size: 6244 NgoMIV G'CCGG_C Cuts at: 3138 3138 Size: 6244 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6244 NotI GC'GGCC_GC Cuts at: 2109 3691 2109 Size: 1582 4662 NspI r_CATG'y Cuts at: 1132 1229 3602 5147 1132 Size: 97 2373 1545 2229 Fragments arranged by size: 2373 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6244 PciI A'CATG_T Cuts at: 5143 5143 Size: 6244 PflMI CCAn_nnn'nTGG Cuts at: 1108 3184 3274 1108 Size: 2076 90 4078 Fragments arranged by size: 4078 2076 90 PmeI GTTT'AAAC Cuts at: 3548 3548 Size: 6244 PmlI CAC'GTG Cuts at: 3112 3112 Size: 6244 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2705 2737 4313 4345 5826 5858 2705 Size: 32 1576 32 1481 32 3091 Fragments arranged by size: 3091 1576 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2392 1613 Size: 779 5465 PsiI TTA'TAA Cuts at: 3814 3814 Size: 6244 PspOMI G'GGCC_C Cuts at: 2072 2072 Size: 6244 PstI C_TGCA'G Cuts at: 830 2088 2767 3242 3596 830 Size: 1258 679 475 354 3478 Fragments arranged by size: 3478 1258 679 475 354 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6244 PvuII CAG'CTG Cuts at: 2064 2131 2359 2989 2064 Size: 67 228 630 5319 Fragments arranged by size: 5319 630 228 67 SacII CC_GC'GG Cuts at: 2109 2109 Size: 6244 SalI G'TCGA_C Cuts at: 1936 3586 1936 Size: 1650 4594 SbfI CC_TGCA'GG Cuts at: 3242 3596 3242 Size: 354 5890 ScaI AGT'ACT Cuts at: 4492 6204 4492 Size: 1712 4532 SexAI A'CCwGG_T Cuts at: 2664 3122 2664 Size: 458 5786 SfcI C'TryA_G Cuts at: 826 1045 2084 2763 3238 3592 4727 5408 5599 Size: 219 1039 679 475 354 1135 681 191 Cuts at: 5599 826 Size: 1471 Fragments arranged by size: 1471 1135 1039 681 679 475 354 219 191 SfoI GGC'GCC Cuts at: 2536 3143 2536 Size: 607 5637 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6244 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6244 SmaI CCC'GGG Cuts at: 1872 2383 2532 3571 1872 Size: 511 149 1039 4545 Fragments arranged by size: 4545 1039 511 149 SmlI C'TyrA_G Cuts at: 820 1017 1943 2216 4348 5249 5511 820 Size: 197 926 273 2132 901 262 1553 Fragments arranged by size: 2132 1553 926 901 273 262 197 SnaBI TAC'GTA Cuts at: 493 4035 493 Size: 3542 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6244 SphI G_CATG'C Cuts at: 1132 3602 1132 Size: 2470 3774 SspI AAT'ATT Cuts at: 5 52 4168 5 Size: 47 4116 2081 Fragments arranged by size: 4116 2081 47 StuI AGG'CCT Cuts at: 1517 3039 1517 Size: 1522 4722 StyI C'CwwG_G Cuts at: 513 1065 3303 3393 3683 513 Size: 552 2238 90 290 3074 Fragments arranged by size: 3074 2238 552 290 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3316 4271 4288 4441 4626 5071 325 Size: 1255 1736 955 17 153 185 445 1498 Fragments arranged by size: 1736 1498 1255 955 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2421 4490 6202 Size: 274 80 33 51 1443 444 2069 1712 Cuts at: 6202 96 Size: 138 Fragments arranged by size: 2069 1712 1443 444 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6204 6236 6204 Size: 32 6212 Tth111I GACn'n_nGTC Cuts at: 1316 2645 1316 Size: 1329 4915 XbaI T'CTAG_A Cuts at: 3580 3580 Size: 6244 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6244 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6244 XmaI C'CCGG_G Cuts at: 1870 2381 2530 3569 1870 Size: 511 149 1039 4545 Fragments arranged by size: 4545 1039 511 149 XmnI GAAnn'nnTTC Cuts at: 4373 4373 Size: 6244 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4052 276 Size: 53 83 186 936 2518 2468 Fragments arranged by size: 2518 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacI SanDI SapI SfiI SrfI SwaI