(Linear) (Six-base) MAPSORT of: KIEE1955.seq Check: 7774 from: 1 to: 1539 KIEE1955.orf With 182 enzymes: SgfI * March 30, 2011 17:32 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 406 964 1184 1539 Size: 406 558 220 355 Fragments arranged by size: 558 406 355 220 AhdI GACnn_n'nnGTC Cuts at: 0 453 1539 Size: 453 1086 AlwNI CAG_nnn'CTG Cuts at: 0 30 62 129 354 1539 Size: 30 32 67 225 1185 Fragments arranged by size: 1185 225 67 32 30 ApaI G_GGCC'C Cuts at: 0 71 1539 Size: 71 1468 ApaLI G'TGCA_C Cuts at: 0 1102 1539 Size: 1102 437 ApoI r'AATT_y Cuts at: 0 763 919 1539 Size: 763 156 620 Fragments arranged by size: 763 620 156 AvaI C'yCGr_G Cuts at: 0 189 376 525 1022 1408 1539 Size: 189 187 149 497 386 131 Fragments arranged by size: 497 386 189 187 149 131 BanI G'GyrC_C Cuts at: 0 529 1136 1539 Size: 529 607 403 Fragments arranged by size: 607 529 403 BanII G_rGCy'C Cuts at: 0 71 148 266 371 1539 Size: 71 77 118 105 1168 Fragments arranged by size: 1168 118 105 77 71 BbeI G_GCGC'C Cuts at: 0 533 1140 1539 Size: 533 607 399 Fragments arranged by size: 607 533 399 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 340 374 482 516 1539 Size: 340 34 108 34 1023 Fragments arranged by size: 1023 340 108 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 1040 1539 Size: 1040 499 BglII A'GATC_T Cuts at: 0 245 1539 Size: 245 1294 BlpI GC'TnA_GC Cuts at: 0 985 1539 Size: 985 554 Bme1580I G_kGCm'C Cuts at: 0 71 1106 1539 Size: 71 1035 433 Fragments arranged by size: 1035 433 71 BmgBI CAC'GTC Cuts at: 0 1014 1179 1539 Size: 1014 165 360 Fragments arranged by size: 1014 360 165 BmrI ACTGGGnnnn_n' Cuts at: 0 1161 1539 Size: 1161 378 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1113 1176 1539 Size: 1113 63 363 Fragments arranged by size: 1113 363 63 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 196 1539 Size: 196 1343 BsaI GGTCTCn'nnnn_ Cuts at: 0 1194 1539 Size: 1194 345 BsaAI yAC'GTr Cuts at: 0 1107 1539 Size: 1107 432 BsaHI Gr'CG_yC Cuts at: 0 454 530 1137 1539 Size: 454 76 607 402 Fragments arranged by size: 607 454 402 76 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 235 265 998 1028 1539 Size: 235 30 733 30 511 Fragments arranged by size: 733 511 235 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 177 346 1539 Size: 177 169 1193 Fragments arranged by size: 1193 177 169 BseYI C'CCAG_C Cuts at: 0 9 129 258 506 1539 Size: 9 120 129 248 1033 Fragments arranged by size: 1033 248 129 120 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 75 271 1402 1539 Size: 75 196 1131 137 Fragments arranged by size: 1131 196 137 75 BsiEI CG_ry'CG Cuts at: 0 107 153 1539 Size: 107 46 1386 Fragments arranged by size: 1386 107 46 BsiHKAI G_wGCw'C Cuts at: 0 1106 1539 Size: 1106 433 BsmI GAATG_Cn' Cuts at: 0 1336 1365 1539 Size: 1336 29 174 Fragments arranged by size: 1336 174 29 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1040 1539 Size: 1040 499 Bsp1286I G_dGCh'C Cuts at: 0 71 148 266 371 1106 1539 Size: 71 77 118 105 735 433 Fragments arranged by size: 735 433 118 105 77 71 BspHI T'CATG_A Cuts at: 0 644 1539 Size: 644 895 BsrBI CCG'CTC Cuts at: 0 543 1539 Size: 543 996 BsrDI GCAATG_nn' Cuts at: 0 1289 1539 Size: 1289 250 BsrFI r'CCGG_y Cuts at: 0 203 272 1133 1539 Size: 203 69 861 406 Fragments arranged by size: 861 406 203 69 BsrGI T'GTAC_A Cuts at: 0 416 1539 Size: 416 1123 BssHII G'CGCG_C Cuts at: 0 605 1539 Size: 605 934 BstAPI GCAn_nnn'nTGC Cuts at: 0 62 354 1539 Size: 62 292 1185 Fragments arranged by size: 1185 292 62 BstYI r'GATC_y Cuts at: 0 245 411 571 1539 Size: 245 166 160 968 Fragments arranged by size: 968 245 166 160 Bsu36I CC'TnA_GG Cuts at: 0 737 1539 Size: 737 802 BtgI C'CryG_G Cuts at: 0 101 1539 Size: 101 1438 BtsI GCAGTG_nn' Cuts at: 0 31 1221 1539 Size: 31 1190 318 Fragments arranged by size: 1190 318 31 DraI TTT'AAA Cuts at: 0 801 1539 Size: 801 738 DrdI GACnn_nn'nnGTC Cuts at: 0 1148 1539 Size: 1148 391 EaeI y'GGCC_r Cuts at: 0 104 150 932 1539 Size: 104 46 782 607 Fragments arranged by size: 782 607 104 46 EagI C'GGCC_G Cuts at: 0 104 150 1539 Size: 104 46 1389 Fragments arranged by size: 1389 104 46 EarI CTCTTCn'nnn_ Cuts at: 0 406 1539 Size: 406 1133 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 30 420 1539 Size: 30 390 1119 Fragments arranged by size: 1119 390 30 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 406 964 1113 1176 1184 1539 Size: 406 558 149 63 8 355 Fragments arranged by size: 558 406 355 149 63 8 EcoO109I rG'GnC_Cy Cuts at: 0 68 1539 Size: 68 1471 EcoRI G'AATT_C Cuts at: 0 763 1539 Size: 763 776 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 264 738 1086 1539 Size: 264 474 348 453 Fragments arranged by size: 474 453 348 264 FspI TGC'GCA Cuts at: 0 1111 1539 Size: 1111 428 FspAI rTGC'GCAy Cuts at: 0 1111 1539 Size: 1111 428 HaeII r_GCGC'y Cuts at: 0 319 533 620 1140 1539 Size: 319 214 87 520 399 Fragments arranged by size: 520 399 319 214 87 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 235 267 439 471 998 1030 1140 1172 Size: 235 32 172 32 527 32 110 32 Cuts at: 1172 1465 1497 1539 Size: 293 32 42 Fragments arranged by size: 527 293 235 172 110 42 32 32 32 32 32 KasI G'GCGC_C Cuts at: 0 529 1136 1539 Size: 529 607 403 Fragments arranged by size: 607 529 403 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 321 437 857 1539 Size: 321 116 420 682 Fragments arranged by size: 682 420 321 116 MslI CAynn'nnrTG Cuts at: 0 643 1358 1539 Size: 643 715 181 Fragments arranged by size: 715 643 181 MspA1I CmG'CkG Cuts at: 0 59 78 97 103 126 354 581 984 Size: 59 19 19 6 23 228 227 403 Cuts at: 984 1539 Size: 555 Fragments arranged by size: 555 403 228 227 59 23 19 19 6 NaeI GCC'GGC Cuts at: 0 1135 1539 Size: 1135 404 NarI GG'CG_CC Cuts at: 0 530 1137 1539 Size: 530 607 402 Fragments arranged by size: 607 530 402 NgoMIV G'CCGG_C Cuts at: 0 1133 1539 Size: 1133 406 NotI GC'GGCC_GC Cuts at: 0 104 1539 Size: 104 1435 PflMI CCAn_nnn'nTGG Cuts at: 0 1179 1269 1539 Size: 1179 90 270 Fragments arranged by size: 1179 270 90 PmlI CAC'GTG Cuts at: 0 1107 1539 Size: 1107 432 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 700 732 1539 Size: 700 32 807 Fragments arranged by size: 807 700 32 PshAI GACnn'nnGTC Cuts at: 0 387 1539 Size: 387 1152 PspOMI G'GGCC_C Cuts at: 0 67 1539 Size: 67 1472 PstI C_TGCA'G Cuts at: 0 83 762 1237 1539 Size: 83 679 475 302 Fragments arranged by size: 679 475 302 83 PvuII CAG'CTG Cuts at: 0 59 126 354 984 1539 Size: 59 67 228 630 555 Fragments arranged by size: 630 555 228 67 59 SacII CC_GC'GG Cuts at: 0 104 1539 Size: 104 1435 SbfI CC_TGCA'GG Cuts at: 0 1237 1539 Size: 1237 302 SexAI A'CCwGG_T Cuts at: 0 659 1117 1539 Size: 659 458 422 Fragments arranged by size: 659 458 422 SfcI C'TryA_G Cuts at: 0 79 758 1233 1539 Size: 79 679 475 306 Fragments arranged by size: 679 475 306 79 SfoI GGC'GCC Cuts at: 0 531 1138 1539 Size: 531 607 401 Fragments arranged by size: 607 531 401 SmaI CCC'GGG Cuts at: 0 378 527 1539 Size: 378 149 1012 Fragments arranged by size: 1012 378 149 SmlI C'TyrA_G Cuts at: 0 211 1539 Size: 211 1328 StuI AGG'CCT Cuts at: 0 1034 1539 Size: 1034 505 StyI C'CwwG_G Cuts at: 0 1298 1388 1539 Size: 1298 90 151 Fragments arranged by size: 1298 151 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1311 1539 Size: 1311 228 TatI w'GTAC_w Cuts at: 0 416 1539 Size: 416 1123 Tth111I GACn'n_nGTC Cuts at: 0 640 1539 Size: 640 899 XmaI C'CCGG_G Cuts at: 0 376 525 1539 Size: 376 149 1014 Fragments arranged by size: 1014 376 149 Enzymes that do cut: AcuI AhdI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstYI Bsu36I BtgI BtsI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI NgoMIV NotI PflMI PmlI PpiI PshAI PspOMI PstI PvuII SacII SbfI SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI Tth111I XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI AloI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BmtI BplI Bpu10I BsaBI BsaWI BsiWI BspEI BspMI BssSI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraIII EcoICRI EcoNI EcoRV FseI HincII HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI NheI NruI NsiI NspI PacI PasI PciI PfoI PmeI PpuMI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI XcmI XhoI XmnI ZraI