Restriction map for
pFN21AE3245
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AviII BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BlpI Bpu1102I BsaAI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI
BstEII BstNI BstPI BstXI Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRII
EcoRV EcoT22I FbaI FseI FspI
HaeII Hin1I HindIII HpaI KasI
KpnI KspI MfeI MluI MroI
MspA1I MunI MvaI NaeI NarI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI PvuII RsrII SacII SalI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI SspBI
StuI SwaI Van91I XcmI XhoI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccII 54 CGCG CG^CG
AlwNI 1526 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 358 ATTAAT AT^TAAT
AsnI 358 ATTAAT AT^TAAT
Asp700I 121 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 328 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 715 CCTAGG C^CTAGG
BalI 1364 TGGCCA TGG^CCA
BamHI 486 GGATCC G^GATCC
BanII 1138 GAGCTC G[GA]GC[CT]^C
BbvI 551 GCTGC GCAGC(8/12)
BglII 969 AGATCT A^GATCT
BlnI 715 CCTAGG C^CTAGG
BpmI 320 CTCCAG CTGGAG(16/14)
BsaI 1544 GAGACC GGTCTC(1/5)
BsiYI 893 CCAAGGCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 893 CCAAGGCTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 1371 GAATGC GAATGC(1/-1)
BspLU11I 1656 ACATGT A^CATGT
BsrFI 8 ACCGGC [GA]^CCGG[CT]
Bst1107I 580 GTATAC GTA^TAC
BstUI 54 CGCG CG^CG
BstZ17I 580 GTATAC GTA^TAC
CfoI 1082 GCGC GCG^C
Cfr10I 8 ACCGGC [GA]^CCGG[CT]
DraII 1442 AGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 1364 TGGCCA [CT]^GGCC[GA]
EcoO109I 1442 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 679 GAATTC G^AATTC
HgaI 52 GACGC GACGC(5/10)
HhaI 1082 GCGC GCG^C
HinP1I 1082 GCGC G^CGC
HincII 519 GTTGAC GT[CT]^[GA]AC
HindII 519 GTTGAC GT[CT]^[GA]AC
MluNI 1364 TGGCCA TGG^CCA
MscI 1364 TGGCCA TGG^CCA
MvnI 54 CGCG CG^CG
NcoI 1205 CCATGG C^CATGG
NheI 589 GCTAGC G^CTAGC
PflFI 328 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1442 AGGTCCC [GA]G^G[AT]CC[CT]
PshBI 358 ATTAAT AT^TAAT
PstI 913 CTGCAG CTGCA^G
SacI 1138 GAGCTC GAGCT^C
SapI 1534 GAAGAGC GCTCTTC(1/4)
SbfI 912 CCTGCAGG CCTGCA^GG
SmaI 37 CCCGGG CCC^GGG
SmlI 1068 CTTGAG C^T[CT][GA]AG
Sse8387I 912 CCTGCAGG CCTGCA^GG
SspI 1387 AATATT AAT^ATT
TseI 551 GCTGC G^C[AT]GC
Tth111I 328 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 415 TCTAGA T^CTAGA
XmaCI 37 CCCGGG C^CCGGG
XmaI 37 CCCGGG C^CCGGG
XmnI 121 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 315 GTCTAC GT^[AC][GT]AC
580 GTATAC
AflIII 1022 ACGTGT A^C[GA][CT]GT
1656 ACATGT
AvaI 37 CCCGGG C^[CT]CG[GA]G
899 CTCGGG
BsaBI 433 GATATGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1186 GATGCTTATC
BsgI 857 CTGCAC GTGCAG(16/14)
1448 CTGCAC
BsoBI 37 CCCGGG C^[CT]CG[GA]G
899 CTCGGG
BspMI 911 ACCTGC ACCTGC(4/8)
915 GCAGGT
FauI 1553 CCCGC CCCGC(4/6)
1134 GCGGG
Fnu4HI 12 GCGGC GC^[ACGT]GC
551 GCTGC
HaeIII 1203 GGCC GG^CC
1365 GGCC
ItaI 12 GCGGC GC^[ACGT]GC
551 GCTGC
MaeII 1022 ACGT A^CGT
1088 ACGT
MamI 433 GATATGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1186 GATGCTTATC
PleI 58 GACTC GAGTC(4/5)
1528 GACTC
SfcI 470 CTGTAG C^T[GA][CT]AG
913 CTGCAG
TaqI 47 TCGA T^CGA
282 TCGA
Tsp45I 403 GTCAC ^GT[GC]AC
1153 GTCAC
TthHB8I 47 TCGA T^CGA
282 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbsI BcnI
BfaI BmyI BpuAI BsaJI BseRI
BsiHKAI BsmAI BsmFI Bsp1286I BspHI
BsrI BssKI BstF5I BstYI Cac8I
Cfr13I DdeI DpnII DsaI EarI
EcoT14I FokI HapII HinfI HpaII
HphI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspI MwoI NciI NdeII NlaIII
NlaIV NspI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TfiI
Tru9I Tsp509I TspRI XhoII