(Circular) (Six-base) MAPSORT of: pFN21AE3245.seq Check: 8249 from: 1 to: 6409 pFN21AE3245 6409 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4219 278 Size: 53 83 186 936 2683 2468 Fragments arranged by size: 2683 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2321 2586 3752 1937 Size: 384 265 1166 4594 Fragments arranged by size: 4594 1166 384 265 Acc65I G'GTAC_C Cuts at: 3730 3730 Size: 6409 AclI AA'CG_TT Cuts at: 1563 4536 4909 1563 Size: 2973 373 3063 Fragments arranged by size: 3063 2973 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3558 4472 5856 6124 1632 Size: 1926 914 1384 268 1917 Fragments arranged by size: 1926 1917 1384 914 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6212 AflIII A'CryG_T Cuts at: 1203 3027 3661 5308 6285 1203 Size: 1824 634 1647 977 1327 Fragments arranged by size: 1824 1647 1327 977 634 AgeI A'CCGG_T Cuts at: 5240 5240 Size: 6409 AhdI GACnn_n'nnGTC Cuts at: 5138 5138 Size: 6409 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6409 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3138 1184 Size: 1954 4455 AlwNI CAG_nnn'CTG Cuts at: 3536 4152 5724 3536 Size: 616 1572 4221 Fragments arranged by size: 4221 1572 616 ApaLI G'TGCA_C Cuts at: 4466 5622 4466 Size: 1156 5253 ApoI r'AATT_y Cuts at: 1493 2348 2628 2684 2984 3152 3651 3718 3944 Size: 855 280 56 300 168 499 67 226 Cuts at: 3944 4096 6205 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 855 499 300 280 226 168 152 67 56 AseI AT'TA_AT Cuts at: 160 1032 2364 4963 160 Size: 872 1332 2599 1606 Fragments arranged by size: 2599 1606 1332 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6409 AvaI C'yCGr_G Cuts at: 1870 1943 2042 2904 3734 1870 Size: 73 99 862 830 4545 Fragments arranged by size: 4545 862 830 99 73 AvrII C'CTAG_G Cuts at: 2720 2720 Size: 6409 BamHI G'GATC_C Cuts at: 2491 3739 4106 2491 Size: 1248 367 4794 Fragments arranged by size: 4794 1248 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3730 5185 618 Size: 325 217 627 1943 1455 1842 Fragments arranged by size: 1943 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3147 3728 1391 Size: 1756 581 4072 Fragments arranged by size: 4072 1756 581 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2099 2133 3689 928 Size: 971 200 34 1556 3648 Fragments arranged by size: 3648 1556 971 200 34 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4600 4634 1903 Size: 34 2663 34 3678 Fragments arranged by size: 3678 2663 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4301 5511 4301 Size: 1210 5199 BclI T'GATC_A Cuts at: 1548 1548 Size: 6409 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5020 136 Size: 107 122 71 631 605 3348 1525 Fragments arranged by size: 3348 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2974 6404 2974 Size: 3430 2979 BlpI GC'TnA_GC Cuts at: 3826 3826 Size: 6409 Bme1580I G_kGCm'C Cuts at: 1790 4470 5626 1790 Size: 2680 1156 2573 Fragments arranged by size: 2680 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3028 1318 Size: 1710 4699 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2221 2227 4140 Size: 416 53 83 399 24 804 6 1913 Cuts at: 4140 5098 442 Size: 958 1753 Fragments arranged by size: 1913 1753 958 804 416 399 83 53 24 6 BmtI G_CTAG'C Cuts at: 1056 2598 1056 Size: 1542 4867 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2310 5069 1239 Size: 1071 2759 2579 Fragments arranged by size: 2759 2579 1071 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3094 4534 5399 5697 3094 Size: 1440 865 298 3806 Fragments arranged by size: 3806 1440 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3543 5072 882 Size: 617 2044 1529 2219 Fragments arranged by size: 2219 2044 1529 617 BsaAI yAC'GTr Cuts at: 493 1204 4200 493 Size: 711 2996 2702 Fragments arranged by size: 2996 2702 711 BsaBI GATnn'nnATC Cuts at: 2442 3195 6403 2442 Size: 753 3208 2448 Fragments arranged by size: 3208 2448 753 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4216 4598 5233 275 Size: 53 83 186 936 2683 382 635 1451 Fragments arranged by size: 2683 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4842 5240 5514 5661 6072 4842 Size: 398 274 147 411 5179 Fragments arranged by size: 5179 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2048 2078 1178 Size: 30 165 30 645 30 5509 Fragments arranged by size: 5509 645 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2056 2192 2195 2198 1186 Size: 870 136 3 3 5397 Fragments arranged by size: 5397 870 136 3 3 BseYI C'CCAG_C Cuts at: 5612 6108 5612 Size: 496 5913 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2847 3438 1163 Size: 573 1111 591 4134 Fragments arranged by size: 4134 1111 591 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3859 4620 5648 1540 Size: 275 186 1858 761 1028 2301 Fragments arranged by size: 2301 1858 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3048 3147 3524 4470 4555 5626 3048 Size: 99 377 946 85 1071 3831 Fragments arranged by size: 3831 1071 946 377 99 85 BsmI GAATG_Cn' Cuts at: 3382 3920 4013 3382 Size: 538 93 5778 Fragments arranged by size: 5778 538 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3048 3147 3524 3728 4470 4555 5626 Size: 399 1258 99 377 204 742 85 1071 Cuts at: 5626 1391 Size: 2174 Fragments arranged by size: 2174 1258 1071 742 399 377 204 99 85 BspEI T'CCGG_A Cuts at: 6072 6072 Size: 6409 BspHI T'CATG_A Cuts at: 2025 2832 3129 4298 6028 2025 Size: 807 297 1169 1730 2406 Fragments arranged by size: 2406 1730 1169 807 297 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2911 2925 3764 844 Size: 2067 14 839 3489 Fragments arranged by size: 3489 2067 839 14 BsrBI CCG'CTC Cuts at: 1394 1823 4296 1394 Size: 429 2473 3507 Fragments arranged by size: 3507 2473 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4904 5078 65 Size: 1183 3656 174 1396 Fragments arranged by size: 3656 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2013 5053 5240 1700 Size: 110 203 3040 187 2869 Fragments arranged by size: 3040 2869 203 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6409 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6409 BssSI C'ACGA_G Cuts at: 4469 5481 4469 Size: 1012 5397 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6409 BstBI TT'CG_AA Cuts at: 5213 5213 Size: 6409 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6409 BstYI r'GATC_y Cuts at: 1972 2491 2974 3163 3739 4106 4491 4508 5960 Size: 519 483 189 576 367 385 17 1452 Cuts at: 5960 6046 6058 6404 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 576 519 483 385 367 346 189 86 17 12 BstZ17I GTA'TAC Cuts at: 2587 2587 Size: 6409 BtgI C'CryG_G Cuts at: 513 1065 2004 3001 3039 3210 3576 513 Size: 552 939 997 38 171 366 3346 Fragments arranged by size: 3346 997 939 552 366 171 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5670 Fragments arranged by size: 5670 683 56 BtsI GCAGTG_nn' Cuts at: 3925 4719 4739 3925 Size: 794 20 5595 Fragments arranged by size: 5595 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6409 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3045 3080 529 Size: 35 2481 35 3858 Fragments arranged by size: 3858 2481 35 35 DraI TTT'AAA Cuts at: 3713 3713 Size: 6409 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5416 809 Size: 507 4100 1802 Fragments arranged by size: 4100 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3369 3856 4745 Size: 54 1425 50 275 18 1539 487 889 Cuts at: 4745 6374 8 Size: 1629 43 Fragments arranged by size: 1629 1539 1425 889 487 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3856 1537 Size: 275 2044 4090 Fragments arranged by size: 4090 2044 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3264 3354 3534 4339 1353 Size: 1911 90 180 805 3423 Fragments arranged by size: 3423 1911 805 180 90 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4996 5370 5516 387 Size: 1064 138 3407 374 146 1280 Fragments arranged by size: 3407 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3145 3145 Size: 6409 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2310 3558 4472 5069 5856 6124 1239 Size: 393 678 1248 914 597 787 268 1524 Fragments arranged by size: 1524 1248 914 787 678 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3448 3448 Size: 6409 EcoRI G'AATT_C Cuts at: 2684 3718 4096 2684 Size: 1034 378 4997 Fragments arranged by size: 4997 1034 378 FspI TGC'GCA Cuts at: 4915 4915 Size: 6409 HaeII r_GCGC'y Cuts at: 1678 5556 1678 Size: 3878 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2048 2080 2779 2811 3518 Size: 32 135 32 476 32 699 32 707 Cuts at: 3518 3550 5050 5082 5124 5156 1373 Size: 32 1500 32 42 32 2626 Fragments arranged by size: 2626 1500 707 699 476 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2526 3753 3999 669 Size: 988 281 588 1227 246 3079 Fragments arranged by size: 3079 1227 988 588 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6409 HpaI GTT'AAC Cuts at: 3999 3999 Size: 6409 KpnI G_GTAC'C Cuts at: 3734 3734 Size: 6409 MfeI C'AATT_G Cuts at: 4008 4008 Size: 6409 MluI A'CGCG_T Cuts at: 6285 6285 Size: 6409 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2212 2322 2469 5523 5707 1305 Size: 907 110 147 3054 184 2007 Fragments arranged by size: 3054 2007 907 184 147 110 MscI TGG'CCA Cuts at: 10 64 1489 1832 3371 10 Size: 54 1425 343 1539 3048 Fragments arranged by size: 3048 1539 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3026 3057 3134 3264 3344 3371 4367 Size: 648 1860 31 77 130 80 27 996 Cuts at: 4367 4726 4885 518 Size: 359 159 2042 Fragments arranged by size: 2042 1860 996 648 359 159 130 80 77 31 27 MspA1I CmG'CkG Cuts at: 1606 3825 4502 5650 5895 1606 Size: 2219 677 1148 245 2120 Fragments arranged by size: 2219 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3210 513 Size: 552 939 1206 3712 Fragments arranged by size: 3712 1206 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6409 NheI G'CTAG_C Cuts at: 1052 2594 1052 Size: 1542 4867 NotI GC'GGCC_GC Cuts at: 3856 3856 Size: 6409 NspI r_CATG'y Cuts at: 1132 1229 2860 2995 3062 3665 3767 5312 1132 Size: 97 1631 135 67 603 102 1545 2229 Fragments arranged by size: 2229 1631 1545 603 135 102 97 67 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6409 PciI A'CATG_T Cuts at: 3661 5308 3661 Size: 1647 4762 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6409 PmeI GTTT'AAAC Cuts at: 3713 3713 Size: 6409 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4478 4510 5991 6023 4478 Size: 32 1481 32 4864 Fragments arranged by size: 4864 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3448 3448 Size: 6409 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6409 PsiI TTA'TAA Cuts at: 2634 3979 2634 Size: 1345 5064 PstI C_TGCA'G Cuts at: 830 2922 3761 830 Size: 2092 839 3478 Fragments arranged by size: 3478 2092 839 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6409 SacI G_AGCT'C Cuts at: 3147 3147 Size: 6409 SalI G'TCGA_C Cuts at: 1936 3751 1936 Size: 1815 4594 SapI GCTCTTCn'nnn_ Cuts at: 3534 3534 Size: 6409 SbfI CC_TGCA'GG Cuts at: 2922 3761 2922 Size: 839 5570 ScaI AGT'ACT Cuts at: 4657 6369 4657 Size: 1712 4697 SfcI C'TryA_G Cuts at: 826 1045 2475 2918 3757 4892 5573 5764 826 Size: 219 1430 443 839 1135 681 191 1471 Fragments arranged by size: 1471 1430 1135 839 681 443 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6409 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6409 SmaI CCC'GGG Cuts at: 1872 2044 3736 1872 Size: 172 1692 4545 Fragments arranged by size: 4545 1692 172 SmlI C'TyrA_G Cuts at: 820 1017 1943 3073 4513 5414 5676 820 Size: 197 926 1130 1440 901 262 1553 Fragments arranged by size: 1553 1440 1130 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4200 493 Size: 3707 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6409 SphI G_CATG'C Cuts at: 1132 3767 1132 Size: 2635 3774 SspI AAT'ATT Cuts at: 5 52 3394 4333 5 Size: 47 3342 939 2081 Fragments arranged by size: 3342 2081 939 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6409 StyI C'CwwG_G Cuts at: 513 1065 2004 2244 2720 2898 3063 3210 3234 Size: 552 939 240 476 178 165 147 24 Cuts at: 3234 3848 513 Size: 614 3074 Fragments arranged by size: 3074 939 614 552 476 240 178 165 147 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3069 4436 4453 4606 4791 5236 325 Size: 1255 1489 1367 17 153 185 445 1498 Fragments arranged by size: 1498 1489 1367 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2931 4655 6367 Size: 274 80 33 51 1443 954 1724 1712 Cuts at: 6367 96 Size: 138 Fragments arranged by size: 1724 1712 1443 954 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6369 6401 6369 Size: 32 6377 Tth111I GACn'n_nGTC Cuts at: 1316 2336 1316 Size: 1020 5389 XbaI T'CTAG_A Cuts at: 2420 3745 2420 Size: 1325 5084 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6409 XmaI C'CCGG_G Cuts at: 1870 2042 3734 1870 Size: 172 1692 4545 Fragments arranged by size: 4545 1692 172 XmnI GAAnn'nnTTC Cuts at: 2130 4538 2130 Size: 2408 4001 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4217 276 Size: 53 83 186 936 2683 2468 Fragments arranged by size: 2683 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI