(Linear) (Six-base) MAPSORT of: KIEE3245.seq Check: 4273 from: 1 to: 1704 KIEE3245.orf With 182 enzymes: SgfI * December 27, 2011 16:34 .. AccI GT'mk_AC Cuts at: 0 316 581 1704 Size: 316 265 1123 Fragments arranged by size: 1123 316 265 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1553 1704 Size: 1553 151 AflIII A'CryG_T Cuts at: 0 1022 1656 1704 Size: 1022 634 48 Fragments arranged by size: 1022 634 48 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1133 1704 Size: 1133 571 AlwNI CAG_nnn'CTG Cuts at: 0 1531 1704 Size: 1531 173 ApoI r'AATT_y Cuts at: 0 343 623 679 979 1147 1646 1704 Size: 343 280 56 300 168 499 58 Fragments arranged by size: 499 343 300 280 168 58 56 AseI AT'TA_AT Cuts at: 0 359 1704 Size: 359 1345 AvaI C'yCGr_G Cuts at: 0 37 899 1704 Size: 37 862 805 Fragments arranged by size: 862 805 37 AvrII C'CTAG_G Cuts at: 0 715 1704 Size: 715 989 BamHI G'GATC_C Cuts at: 0 486 1704 Size: 486 1218 BanII G_rGCy'C Cuts at: 0 1142 1704 Size: 1142 562 BbsI GAAGACnn'nnnn_ Cuts at: 0 94 128 1684 1704 Size: 94 34 1556 20 Fragments arranged by size: 1556 94 34 20 BglII A'GATC_T Cuts at: 0 969 1704 Size: 969 735 BmgBI CAC'GTC Cuts at: 0 1023 1704 Size: 1023 681 BmrI ACTGGGnnnn_n' Cuts at: 0 216 222 1704 Size: 216 6 1482 Fragments arranged by size: 1482 216 6 BmtI G_CTAG'C Cuts at: 0 593 1704 Size: 593 1111 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 305 1704 Size: 305 1399 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1089 1704 Size: 1089 615 BsaI GGTCTCn'nnnn_ Cuts at: 0 1538 1704 Size: 1538 166 BsaBI GATnn'nnATC Cuts at: 0 437 1190 1704 Size: 437 753 514 Fragments arranged by size: 753 514 437 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 43 73 1704 Size: 43 30 1631 Fragments arranged by size: 1631 43 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 51 187 190 193 1704 Size: 51 136 3 3 1511 Fragments arranged by size: 1511 136 51 3 3 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 842 1433 1704 Size: 842 591 271 Fragments arranged by size: 842 591 271 BsiHKAI G_wGCw'C Cuts at: 0 1043 1142 1519 1704 Size: 1043 99 377 185 Fragments arranged by size: 1043 377 185 99 BsmI GAATG_Cn' Cuts at: 0 1377 1704 Size: 1377 327 Bsp1286I G_dGCh'C Cuts at: 0 1043 1142 1519 1704 Size: 1043 99 377 185 Fragments arranged by size: 1043 377 185 99 BspHI T'CATG_A Cuts at: 0 20 827 1124 1704 Size: 20 807 297 580 Fragments arranged by size: 807 580 297 20 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 906 920 1704 Size: 906 14 784 Fragments arranged by size: 906 784 14 BsrFI r'CCGG_y Cuts at: 0 8 1704 Size: 8 1696 BstYI r'GATC_y Cuts at: 0 486 969 1158 1704 Size: 486 483 189 546 Fragments arranged by size: 546 486 483 189 BstZ17I GTA'TAC Cuts at: 0 582 1704 Size: 582 1122 BtgI C'CryG_G Cuts at: 0 996 1034 1205 1571 1704 Size: 996 38 171 366 133 Fragments arranged by size: 996 366 171 133 38 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1040 1075 1704 Size: 1040 35 629 Fragments arranged by size: 1040 629 35 EaeI y'GGCC_r Cuts at: 0 1364 1704 Size: 1364 340 EarI CTCTTCn'nnn_ Cuts at: 0 1259 1349 1529 1704 Size: 1259 90 180 175 Fragments arranged by size: 1259 180 175 90 EcoICRI GAG'CTC Cuts at: 0 1140 1704 Size: 1140 564 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 305 1553 1704 Size: 305 1248 151 Fragments arranged by size: 1248 305 151 EcoO109I rG'GnC_Cy Cuts at: 0 1443 1704 Size: 1443 261 EcoRI G'AATT_C Cuts at: 0 679 1704 Size: 679 1025 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 43 75 774 806 1513 1545 1704 Size: 43 32 699 32 707 32 159 Fragments arranged by size: 707 699 159 43 32 32 32 HincII GTy'rAC Cuts at: 0 521 1704 Size: 521 1183 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 207 317 464 1704 Size: 207 110 147 1240 Fragments arranged by size: 1240 207 147 110 MscI TGG'CCA Cuts at: 0 1366 1704 Size: 1366 338 MslI CAynn'nnrTG Cuts at: 0 1021 1052 1129 1259 1339 1366 1704 Size: 1021 31 77 130 80 27 338 Fragments arranged by size: 1021 338 130 80 77 31 27 NcoI C'CATG_G Cuts at: 0 1205 1704 Size: 1205 499 NheI G'CTAG_C Cuts at: 0 589 1704 Size: 589 1115 NspI r_CATG'y Cuts at: 0 855 990 1057 1660 1704 Size: 855 135 67 603 44 Fragments arranged by size: 855 603 135 67 44 PciI A'CATG_T Cuts at: 0 1656 1704 Size: 1656 48 PpuMI rG'GwC_Cy Cuts at: 0 1443 1704 Size: 1443 261 PsiI TTA'TAA Cuts at: 0 629 1704 Size: 629 1075 PstI C_TGCA'G Cuts at: 0 917 1704 Size: 917 787 SacI G_AGCT'C Cuts at: 0 1142 1704 Size: 1142 562 SapI GCTCTTCn'nnn_ Cuts at: 0 1529 1704 Size: 1529 175 SbfI CC_TGCA'GG Cuts at: 0 917 1704 Size: 917 787 SfcI C'TryA_G Cuts at: 0 470 913 1704 Size: 470 443 791 Fragments arranged by size: 791 470 443 SmaI CCC'GGG Cuts at: 0 39 1704 Size: 39 1665 SmlI C'TyrA_G Cuts at: 0 1068 1704 Size: 1068 636 SspI AAT'ATT Cuts at: 0 1389 1704 Size: 1389 315 StyI C'CwwG_G Cuts at: 0 239 715 893 1058 1205 1229 1704 Size: 239 476 178 165 147 24 475 Fragments arranged by size: 476 475 239 178 165 147 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1064 1704 Size: 1064 640 TatI w'GTAC_w Cuts at: 0 926 1704 Size: 926 778 Tth111I GACn'n_nGTC Cuts at: 0 331 1704 Size: 331 1373 XbaI T'CTAG_A Cuts at: 0 415 1704 Size: 415 1289 XmaI C'CCGG_G Cuts at: 0 37 1704 Size: 37 1667 XmnI GAAnn'nnTTC Cuts at: 0 125 1704 Size: 125 1579 Enzymes that do cut: AccI AcuI AflIII AlfI AlwNI ApoI AseI AvaI AvrII BamHI BanII BbsI BglII BmgBI BmrI BmtI BpmI BpuEI BsaI BsaBI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI BstYI BstZ17I BtgI CspCI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI Hin4I HincII MmeI MscI MslI NcoI NheI NspI PciI PpuMI PsiI PstI SacI SapI SbfI SfcI SmaI SmlI SspI StyI TaqII TatI Tth111I XbaI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApaI ApaLI AscI AsiSI BaeI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BplI Bpu10I BsaAI BsaHI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI Bsu36I BtgZI BtsI ClaI DraI DraIII DrdI EagI EciI EcoNI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PasI PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI StuI SwaI TstI XcmI XhoI ZraI