Restriction map for
pFN21AB9595
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# NO CUT ENZYMES ( 107 enzymes) #
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AclI AcsI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AseI AsnI Asp700I AspEI AspI
AvrII BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BpuAI BsaBI BsiWI
BsmBI BsmI Bsp1407I BspDI BsrDI
BsrGI Bst1107I BstAPI BstBI BstYI
BstZ17I Bsu36I ClaI DraI DrdI
Eam1105I EarI Eco47III Eco81I EcoRI
EcoRV EcoT22I FbaI HincII HindII
HindIII HpaI Ksp632I MaeI MamI
MfeI MflI MluI MseI MunI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII SalI SapI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 942 GACGTC GACGT^C
Acc65I 1418 GGTACC G^GTACC
AccI 1639 GTCTAC GT^[AC][GT]AC
AccIII 1627 TCCGGA T^CCGGA
AflIII 479 ACATGT A^C[GA][CT]GT
ApaI 193 GGGCCC GGGCC^C
AscI 1240 GGCGCGCC GG^CGCGCC
Asp718I 1418 GGTACC G^GTACC
BalI 266 TGGCCA TGG^CCA
BsaAI 693 TACGTG [CT]AC^GT[GA]
BsaI 1439 GAGACC GGTCTC(1/5)
BseAI 1627 TCCGGA T^CCGGA
BseRI 550 GAGGAG GAGGAG(10/8)
BsgI 565 CTGCAC GTGCAG(16/14)
BspEI 1627 TCCGGA T^CCGGA
BspHI 938 TCATGA T^CATGA
BspLU11I 479 ACATGT A^CATGT
BspMI 1187 ACCTGC ACCTGC(4/8)
BsrBI 844 CCGCTC CCGCTC(-3/-3)
BssSI 1413 CTCGTG CACGAG(-5/-1)
BstEII 1305 GGTCACC G^GT[ACGT]ACC
BstPI 1305 GGTCACC G^GT[ACGT]ACC
CpoI 642 CGGTCCG CG^G[AT]CCG
EcoNI 1422 CCTGAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1305 GGTCACC G^GT[ACGT]ACC
FseI 1374 GGCCGGCC GGCCGG^CC
KpnI 1418 GGTACC GGTAC^C
MluNI 266 TGGCCA TGG^CCA
MroI 1627 TCCGGA T^CCGGA
MscI 266 TGGCCA TGG^CCA
NcoI 263 CCATGG C^CATGG
NspI 479 ACATGT [GA]CATG^[CT]
PpuMI 1171 GGGACCT [GA]G^G[AT]CC[CT]
PstI 1092 CTGCAG CTGCA^G
RcaI 938 TCATGA T^CATGA
RsrII 642 CGGTCCG CG^G[AT]CCG
SmlI 571 CTCAAG C^T[CT][GA]AG
TfiI 865 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AlwI 1028 GGATC GGATC(4/5)
1544 GGATC
AspHI 979 GAGCTC G[AT]GC[AT]^C
1071 GAGCTC
AviII 150 TGCGCA TGC^GCA
662 TGCGCA
BbeI 1051 GGCGCC GGCGC^C
1165 GGCGCC
BlpI 846 GCTCAGC GC^T[ACGT]AGC
981 GCTCAGC
BpmI 1104 CTCCAG CTGGAG(16/14)
1562 CTCCAG
Bpu1102I 846 GCTCAGC GC^T[ACGT]AGC
981 GCTCAGC
BsaWI 1302 TCCGGT [AT]^CCGG[AT]
1627 TCCGGA
BsiHKAI 979 GAGCTC G[AT]GC[AT]^C
1071 GAGCTC
BsmAI 1439 GAGAC GTCTC(1/5)
1611 GTCTC
BstXI 29 CCAACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
218 CCAAGGACATGG
CelII 846 GCTCAGC GC^T[ACGT]AGC
981 GCTCAGC
DraII 193 GGGCCCT [GA]G^G[ACGT]CC[CT]
1171 GGGACCT
DraIII 1410 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
1513 CACTCGGTG
EcoO109I 193 GGGCCCT [GA]G^G[ACGT]CC[CT]
1171 GGGACCT
FspI 150 TGCGCA TGC^GCA
662 TGCGCA
KasI 1051 GGCGCC G^GCGCC
1165 GGCGCC
MboII 291 GAAGA GAAGA(8/7)
525 GAAGA
NarI 1051 GGCGCC GG^CGCC
1165 GGCGCC
SacI 979 GAGCTC GAGCT^C
1071 GAGCTC
SfiI 87 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
999 GGCCTCGGCGGCC
SfoI 1051 GGCGCC GGC^GCC
1165 GGCGCC
TspRI 1180 CACTG CA[GC]TG[ACGT][ACGT]^
1565 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AvaI AvaII BanI BanII
BbvI BcnI BglI BmyI BsaHI
BsaJI BsiEI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EaeI EagI EclXI Eco52I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KspI MaeII MaeIII MboI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NotI PleI
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StyI TaqI
TseI Tsp45I TthHB8I XmaCI XmaI