(Circular) (Six-base) MAPSORT of: pFN21AB9595.seq Check: 378 from: 1 to: 6364 pFN21AB9595 6364 bp With 182 enzymes: SgfI * September 8, 2014 18:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2951 4174 278 Size: 53 83 186 936 1415 1223 2468 Fragments arranged by size: 2468 1415 1223 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3645 3707 1937 Size: 1708 62 4594 Fragments arranged by size: 4594 1708 62 Acc65I G'GTAC_C Cuts at: 3423 3685 3423 Size: 262 6102 AclI AA'CG_TT Cuts at: 1563 4491 4864 1563 Size: 2928 373 3063 Fragments arranged by size: 3063 2928 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2911 4427 5811 6079 1632 Size: 1279 1516 1384 268 1917 Fragments arranged by size: 1917 1516 1384 1279 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6167 AflIII A'CryG_T Cuts at: 1203 2484 5263 6240 1203 Size: 1281 2779 977 1327 Fragments arranged by size: 2779 1327 1281 977 AgeI A'CCGG_T Cuts at: 5195 5195 Size: 6364 AhdI GACnn_n'nnGTC Cuts at: 5093 5093 Size: 6364 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6364 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2522 1184 Size: 1338 5026 AlwNI CAG_nnn'CTG Cuts at: 2457 2722 3619 4107 5679 2457 Size: 265 897 488 1572 3142 Fragments arranged by size: 3142 1572 897 488 265 ApaI G_GGCC'C Cuts at: 2202 2202 Size: 6364 ApaLI G'TGCA_C Cuts at: 4421 5577 4421 Size: 1156 5208 ApoI r'AATT_y Cuts at: 1493 3673 3899 4051 6160 1493 Size: 2180 226 152 2109 1697 Fragments arranged by size: 2180 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 3246 3246 Size: 6364 AseI AT'TA_AT Cuts at: 160 1032 4918 160 Size: 872 3886 1606 Fragments arranged by size: 3886 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6364 AvaI C'yCGr_G Cuts at: 1870 1943 2173 2824 3124 3251 3496 3689 1870 Size: 73 230 651 300 127 245 193 4545 Fragments arranged by size: 4545 651 300 245 230 193 127 73 BamHI G'GATC_C Cuts at: 3694 4061 3694 Size: 367 5997 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2050 2419 2764 3056 3170 Size: 325 217 627 263 369 345 292 114 Cuts at: 3170 3423 3685 5140 618 Size: 253 262 1455 1842 Fragments arranged by size: 1842 1455 627 369 345 325 292 263 262 253 217 114 BanII G_rGCy'C Cuts at: 1391 2202 2783 2988 3080 3683 1391 Size: 811 581 205 92 603 4072 Fragments arranged by size: 4072 811 603 581 205 92 BbeI G_GCGC'C Cuts at: 3060 3174 3060 Size: 114 6250 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5393 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4555 4589 1903 Size: 34 50 34 2534 34 3678 Fragments arranged by size: 3678 2534 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4256 5466 4256 Size: 1210 5154 BclI T'GATC_A Cuts at: 1548 1548 Size: 6364 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2099 2126 Size: 107 122 71 631 605 337 90 27 Cuts at: 2126 2826 3011 4975 136 Size: 700 185 1964 1525 Fragments arranged by size: 1964 1525 700 631 605 337 185 122 107 90 71 27 BglII A'GATC_T Cuts at: 6359 6359 Size: 6364 BlpI GC'TnA_GC Cuts at: 2852 2987 3781 2852 Size: 135 794 5435 Fragments arranged by size: 5435 794 135 Bme1580I G_kGCm'C Cuts at: 1790 2055 2202 2705 2769 4425 5581 1790 Size: 265 147 503 64 1656 1156 2573 Fragments arranged by size: 2573 1656 1156 503 265 147 64 BmgBI CAC'GTC Cuts at: 1318 3630 1318 Size: 2312 4052 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4095 5053 442 Size: 416 53 83 399 24 2678 958 1753 Fragments arranged by size: 2678 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6364 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3007 3007 Size: 6364 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3094 3552 5024 1239 Size: 1855 458 1472 2579 Fragments arranged by size: 2579 1855 1472 458 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2561 4489 5354 5652 2561 Size: 1928 865 298 3273 Fragments arranged by size: 3273 1928 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3438 5027 882 Size: 617 1939 1589 2219 Fragments arranged by size: 2219 1939 1589 617 BsaAI yAC'GTr Cuts at: 493 1204 2700 4155 493 Size: 711 1496 1455 2702 Fragments arranged by size: 2702 1496 1455 711 BsaBI GATnn'nnATC Cuts at: 6358 6358 Size: 6364 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2948 3057 3171 4171 Size: 53 83 186 936 1415 109 114 1000 Cuts at: 4171 4553 5188 275 Size: 382 635 1451 Fragments arranged by size: 1451 1415 1000 936 635 382 186 114 109 83 53 BsaWI w'CCGG_w Cuts at: 3307 3632 4797 5195 5469 5616 6027 3307 Size: 325 1165 398 274 147 411 3644 Fragments arranged by size: 3644 1165 411 398 325 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2459 2489 3627 3657 1178 Size: 30 165 30 1056 30 1138 30 3885 Fragments arranged by size: 3885 1138 1056 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2570 1186 Size: 1384 4980 BseYI C'CCAG_C Cuts at: 2715 5567 6063 2715 Size: 2852 496 3016 Fragments arranged by size: 3016 2852 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2555 1163 Size: 573 819 4972 Fragments arranged by size: 4972 819 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2094 2103 2599 2733 2849 2979 Size: 275 186 93 9 496 134 116 130 Cuts at: 2979 3015 3219 3273 3486 3814 4575 5603 1540 Size: 36 204 54 213 328 761 1028 2301 Fragments arranged by size: 2301 1028 761 496 328 275 213 204 186 134 130 116 93 54 36 9 BsiHKAI G_wGCw'C Cuts at: 2988 3080 4425 4510 5581 2988 Size: 92 1345 85 1071 3771 Fragments arranged by size: 3771 1345 1071 92 85 BsmI GAATG_Cn' Cuts at: 3875 3968 3875 Size: 93 6271 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2202 2705 2769 2783 2988 3080 Size: 399 265 147 503 64 14 205 92 Cuts at: 3080 3683 4425 4510 5581 1391 Size: 603 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 603 503 399 265 205 147 92 85 64 14 BspEI T'CCGG_A Cuts at: 3632 6027 3632 Size: 2395 3969 BspHI T'CATG_A Cuts at: 2943 4253 5983 2943 Size: 1310 1730 3324 Fragments arranged by size: 3324 1730 1310 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3201 3719 844 Size: 2357 518 3489 Fragments arranged by size: 3489 2357 518 BsrBI CCG'CTC Cuts at: 1394 1823 2851 4251 1394 Size: 429 1028 1400 3507 Fragments arranged by size: 3507 1400 1028 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4859 5033 65 Size: 1183 3611 174 1396 Fragments arranged by size: 3611 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2125 3218 3380 5008 5195 1700 Size: 110 315 1093 162 1628 187 2869 Fragments arranged by size: 2869 1628 1093 315 187 162 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6364 BssHII G'CGCG_C Cuts at: 1925 2012 3025 3246 1925 Size: 87 1013 221 5043 Fragments arranged by size: 5043 1013 221 87 BssSI C'ACGA_G Cuts at: 3418 4424 5436 3418 Size: 1006 1012 4346 Fragments arranged by size: 4346 1012 1006 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6364 BstBI TT'CG_AA Cuts at: 5168 5168 Size: 6364 BstEII G'GTnAC_C Cuts at: 1184 3310 1184 Size: 2126 4238 BstXI CCAn_nnnn'nTGG Cuts at: 2041 2230 2041 Size: 189 6175 BstYI r'GATC_y Cuts at: 1972 3694 4061 4446 4463 5915 6001 6013 6359 Size: 1722 367 385 17 1452 86 12 346 Cuts at: 6359 1972 Size: 1977 Fragments arranged by size: 1977 1722 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2094 2268 2760 2979 3153 3273 513 Size: 552 1029 174 492 219 174 120 3604 Fragments arranged by size: 3604 1029 552 492 219 174 174 120 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2239 487 Size: 683 56 1013 4612 Fragments arranged by size: 4612 1013 683 56 BtsI GCAGTG_nn' Cuts at: 3184 3880 4674 4694 3184 Size: 696 794 20 4854 Fragments arranged by size: 4854 794 696 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6364 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6329 DraI TTT'AAA Cuts at: 3668 3668 Size: 6364 DraIII CAC_nnn'GTG Cuts at: 3420 3523 3420 Size: 103 6261 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5371 809 Size: 507 4055 1802 Fragments arranged by size: 4055 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 2100 2271 Size: 54 1425 50 275 18 261 9 171 Cuts at: 2271 2596 2730 2846 2976 3012 3070 3216 3270 Size: 325 134 116 130 36 58 146 54 Cuts at: 3270 3382 3483 3811 4700 6329 8 Size: 112 101 328 889 1629 43 Fragments arranged by size: 1629 1425 889 328 325 275 261 171 146 134 130 116 112 101 58 54 54 50 43 36 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2091 2100 2596 2730 2846 2976 3012 Size: 275 279 9 496 134 116 130 36 Cuts at: 3012 3216 3270 3483 3811 1537 Size: 204 54 213 328 4090 Fragments arranged by size: 4090 496 328 279 275 213 204 134 130 116 54 36 9 EarI CTCTTCn'nnn_ Cuts at: 1353 4294 1353 Size: 2941 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2372 2887 3456 4951 5325 5471 Size: 1064 138 783 515 569 1495 374 146 Cuts at: 5471 387 Size: 1280 Fragments arranged by size: 1495 1280 1064 783 569 515 374 146 138 EcoICRI GAG'CTC Cuts at: 2986 3078 2986 Size: 92 6272 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2911 3094 3552 4427 5024 5811 6079 Size: 393 1279 183 458 875 597 787 268 Cuts at: 6079 1239 Size: 1524 Fragments arranged by size: 1524 1279 875 787 597 458 393 268 183 EcoNI CCTnn'n_nnAGG Cuts at: 3431 3431 Size: 6364 EcoO109I rG'GnC_Cy Cuts at: 2199 3177 2199 Size: 978 5386 EcoRI G'AATT_C Cuts at: 3673 4051 3673 Size: 378 5986 FseI GG_CCGG'CC Cuts at: 3384 3384 Size: 6364 FspI TGC'GCA Cuts at: 2157 2669 4870 2157 Size: 512 2201 3651 Fragments arranged by size: 3651 2201 512 FspAI rTGC'GCAy Cuts at: 2669 2669 Size: 6364 HaeII r_GCGC'y Cuts at: 1678 2524 2757 3053 3060 3174 5511 1678 Size: 846 233 296 7 114 2337 2531 Fragments arranged by size: 2531 2337 846 296 233 114 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2459 2491 3627 3659 5005 Size: 32 135 32 887 32 1136 32 1346 Cuts at: 5005 5037 5079 5111 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1346 1136 887 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3708 3954 669 Size: 988 281 1770 246 3079 Fragments arranged by size: 3079 1770 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6364 HpaI GTT'AAC Cuts at: 3954 3954 Size: 6364 KasI G'GCGC_C Cuts at: 3056 3170 3056 Size: 114 6250 KpnI G_GTAC'C Cuts at: 3427 3689 3427 Size: 262 6102 MfeI C'AATT_G Cuts at: 3963 3963 Size: 6364 MluI A'CGCG_T Cuts at: 6240 6240 Size: 6364 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5478 5662 1305 Size: 4173 184 2007 Fragments arranged by size: 4173 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2273 10 Size: 54 1425 343 441 4101 Fragments arranged by size: 4101 1425 441 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2120 2138 2267 4322 4681 4840 518 Size: 648 954 18 129 2055 359 159 2042 Fragments arranged by size: 2055 2042 954 648 359 159 129 18 MspA1I CmG'CkG Cuts at: 1606 2096 2748 2762 2811 2981 3046 3155 3780 Size: 490 652 14 49 170 65 109 625 Cuts at: 3780 4457 5605 5850 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 652 625 490 245 170 109 65 49 14 NaeI GCC'GGC Cuts at: 2127 3220 3382 2127 Size: 1093 162 5109 Fragments arranged by size: 5109 1093 162 NarI GG'CG_CC Cuts at: 3057 3171 3057 Size: 114 6250 NcoI C'CATG_G Cuts at: 513 1065 2268 513 Size: 552 1203 4609 Fragments arranged by size: 4609 1203 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6364 NgoMIV G'CCGG_C Cuts at: 2125 3218 3380 2125 Size: 1093 162 5109 Fragments arranged by size: 5109 1093 162 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6364 NotI GC'GGCC_GC Cuts at: 2091 2846 2976 3012 3483 3811 2091 Size: 755 130 36 471 328 4644 Fragments arranged by size: 4644 755 471 328 130 36 NspI r_CATG'y Cuts at: 1132 1229 2488 3722 5267 1132 Size: 97 1259 1234 1545 2229 Fragments arranged by size: 2229 1545 1259 1234 97 PasI CC'CwG_GG Cuts at: 1348 2041 2740 1348 Size: 693 699 4972 Fragments arranged by size: 4972 699 693 PciI A'CATG_T Cuts at: 2484 5263 2484 Size: 2779 3585 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6364 PfoI T'CCnGG_A Cuts at: 2673 2673 Size: 6364 PmeI GTTT'AAAC Cuts at: 3668 3668 Size: 6364 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3503 3535 4433 4465 5946 5978 3503 Size: 32 898 32 1481 32 3889 Fragments arranged by size: 3889 1481 898 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3177 3177 Size: 6364 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6364 PsiI TTA'TAA Cuts at: 3934 3934 Size: 6364 PspOMI G'GGCC_C Cuts at: 2198 2198 Size: 6364 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2300 2332 2300 Size: 32 6332 PstI C_TGCA'G Cuts at: 830 3101 3716 830 Size: 2271 615 3478 Fragments arranged by size: 3478 2271 615 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6364 RsrII CG'GwC_CG Cuts at: 2648 2648 Size: 6364 SacI G_AGCT'C Cuts at: 2988 3080 2988 Size: 92 6272 SacII CC_GC'GG Cuts at: 2097 2763 2982 3156 2097 Size: 666 219 174 5305 Fragments arranged by size: 5305 666 219 174 SalI G'TCGA_C Cuts at: 1936 3706 1936 Size: 1770 4594 SbfI CC_TGCA'GG Cuts at: 3716 3716 Size: 6364 ScaI AGT'ACT Cuts at: 4612 6324 4612 Size: 1712 4652 SfcI C'TryA_G Cuts at: 826 1045 2245 3085 3097 3652 3712 4847 5528 Size: 219 1200 840 12 555 60 1135 681 Cuts at: 5528 5719 826 Size: 191 1471 Fragments arranged by size: 1471 1200 1135 840 681 555 219 191 60 12 SfiI GGCCn_nnn'nGGCC Cuts at: 2099 3011 2099 Size: 912 5452 SfoI GGC'GCC Cuts at: 3058 3172 3058 Size: 114 6250 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6364 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6364 SmaI CCC'GGG Cuts at: 1872 2175 2826 3253 3498 3691 1872 Size: 303 651 427 245 193 4545 Fragments arranged by size: 4545 651 427 303 245 193 SmlI C'TyrA_G Cuts at: 820 1017 1943 2576 4468 5369 5631 820 Size: 197 926 633 1892 901 262 1553 Fragments arranged by size: 1892 1553 926 901 633 262 197 SnaBI TAC'GTA Cuts at: 493 4155 493 Size: 3662 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6364 SphI G_CATG'C Cuts at: 1132 3722 1132 Size: 2590 3774 SspI AAT'ATT Cuts at: 5 52 4288 5 Size: 47 4236 2081 Fragments arranged by size: 4236 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6364 StyI C'CwwG_G Cuts at: 513 1065 2223 2268 3403 3803 513 Size: 552 1158 45 1135 400 3074 Fragments arranged by size: 3074 1158 1135 552 400 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2702 4391 4408 4561 4746 5191 325 Size: 1255 1122 1689 17 153 185 445 1498 Fragments arranged by size: 1689 1498 1255 1122 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2142 4610 6322 Size: 274 80 33 51 1443 165 2468 1712 Cuts at: 6322 96 Size: 138 Fragments arranged by size: 2468 1712 1443 274 165 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6324 6356 6324 Size: 32 6332 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6364 XbaI T'CTAG_A Cuts at: 3700 3700 Size: 6364 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6364 XmaI C'CCGG_G Cuts at: 1870 2173 2824 3251 3496 3689 1870 Size: 303 651 427 245 193 4545 Fragments arranged by size: 4545 651 427 303 245 193 XmnI GAAnn'nnTTC Cuts at: 4493 4493 Size: 6364 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2949 4172 276 Size: 53 83 186 936 1415 1223 2468 Fragments arranged by size: 2468 1415 1223 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI RsrII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI NruI NsiI PacI PmlI PspXI PvuII SanDI SapI SexAI SrfI SwaI XcmI