(Linear) (Six-base) MAPSORT of: KIBB9595.seq Check: 7820 from: 1 to: 1659 KIBB9595.orf With 182 enzymes: SgfI * September 8, 2014 17:51 .. AatII G_ACGT'C Cuts at: 0 946 1659 Size: 946 713 AccI GT'mk_AC Cuts at: 0 1640 1659 Size: 1640 19 Acc65I G'GTAC_C Cuts at: 0 1418 1659 Size: 1418 241 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 906 1659 Size: 906 753 AflIII A'CryG_T Cuts at: 0 479 1659 Size: 479 1180 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 517 1659 Size: 517 1142 AlwNI CAG_nnn'CTG Cuts at: 0 452 717 1614 1659 Size: 452 265 897 45 Fragments arranged by size: 897 452 265 45 ApaI G_GGCC'C Cuts at: 0 197 1659 Size: 197 1462 AscI GG'CGCG_CC Cuts at: 0 1241 1659 Size: 1241 418 AvaI C'yCGr_G Cuts at: 0 168 819 1119 1246 1491 1659 Size: 168 651 300 127 245 168 Fragments arranged by size: 651 300 245 168 168 127 BanI G'GyrC_C Cuts at: 0 45 414 759 1051 1165 1418 1659 Size: 45 369 345 292 114 253 241 Fragments arranged by size: 369 345 292 253 241 114 45 BanII G_rGCy'C Cuts at: 0 197 778 983 1075 1659 Size: 197 581 205 92 584 Fragments arranged by size: 584 581 205 197 92 BbeI G_GCGC'C Cuts at: 0 1055 1169 1659 Size: 1055 114 490 Fragments arranged by size: 1055 490 114 BglI GCCn_nnn'nGGC Cuts at: 0 94 121 821 1006 1659 Size: 94 27 700 185 653 Fragments arranged by size: 700 653 185 94 27 BlpI GC'TnA_GC Cuts at: 0 847 982 1659 Size: 847 135 677 Fragments arranged by size: 847 677 135 Bme1580I G_kGCm'C Cuts at: 0 50 197 700 764 1659 Size: 50 147 503 64 895 Fragments arranged by size: 895 503 147 64 50 BmgBI CAC'GTC Cuts at: 0 1625 1659 Size: 1625 34 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1002 1659 Size: 1002 657 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1089 1547 1659 Size: 1089 458 112 Fragments arranged by size: 1089 458 112 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 556 1659 Size: 556 1103 BsaI GGTCTCn'nnnn_ Cuts at: 0 1433 1659 Size: 1433 226 BsaAI yAC'GTr Cuts at: 0 695 1659 Size: 695 964 BsaHI Gr'CG_yC Cuts at: 0 943 1052 1166 1659 Size: 943 109 114 493 Fragments arranged by size: 943 493 114 109 BsaWI w'CCGG_w Cuts at: 0 1302 1627 1659 Size: 1302 325 32 Fragments arranged by size: 1302 325 32 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 454 484 1622 1652 1659 Size: 454 30 1138 30 7 Fragments arranged by size: 1138 454 30 30 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 565 1659 Size: 565 1094 BseYI C'CCAG_C Cuts at: 0 710 1659 Size: 710 949 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 550 1659 Size: 550 1109 BsiEI CG_ry'CG Cuts at: 0 89 98 594 728 844 974 1010 1214 Size: 89 9 496 134 116 130 36 204 Cuts at: 1214 1268 1481 1659 Size: 54 213 178 Fragments arranged by size: 496 213 204 178 134 130 116 89 54 36 9 BsiHKAI G_wGCw'C Cuts at: 0 983 1075 1659 Size: 983 92 584 Fragments arranged by size: 983 584 92 Bsp1286I G_dGCh'C Cuts at: 0 50 197 700 764 778 983 1075 1659 Size: 50 147 503 64 14 205 92 584 Fragments arranged by size: 584 503 205 147 92 64 50 14 BspEI T'CCGG_A Cuts at: 0 1627 1659 Size: 1627 32 BspHI T'CATG_A Cuts at: 0 938 1659 Size: 938 721 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1196 1659 Size: 1196 463 BsrBI CCG'CTC Cuts at: 0 846 1659 Size: 846 813 BsrFI r'CCGG_y Cuts at: 0 120 1213 1375 1659 Size: 120 1093 162 284 Fragments arranged by size: 1093 284 162 120 BssHII G'CGCG_C Cuts at: 0 7 1020 1241 1659 Size: 7 1013 221 418 Fragments arranged by size: 1013 418 221 7 BssSI C'ACGA_G Cuts at: 0 1413 1659 Size: 1413 246 BstEII G'GTnAC_C Cuts at: 0 1305 1659 Size: 1305 354 BstXI CCAn_nnnn'nTGG Cuts at: 0 36 225 1659 Size: 36 189 1434 Fragments arranged by size: 1434 189 36 BtgI C'CryG_G Cuts at: 0 89 263 755 974 1148 1268 1659 Size: 89 174 492 219 174 120 391 Fragments arranged by size: 492 391 219 174 174 120 89 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 234 1659 Size: 234 1425 BtsI GCAGTG_nn' Cuts at: 0 1179 1659 Size: 1179 480 DraIII CAC_nnn'GTG Cuts at: 0 1415 1518 1659 Size: 1415 103 141 Fragments arranged by size: 1415 141 103 EaeI y'GGCC_r Cuts at: 0 86 95 266 591 725 841 971 1007 Size: 86 9 171 325 134 116 130 36 Cuts at: 1007 1065 1211 1265 1377 1478 1659 Size: 58 146 54 112 101 181 Fragments arranged by size: 325 181 171 146 134 130 116 112 101 86 58 54 36 9 EagI C'GGCC_G Cuts at: 0 86 95 591 725 841 971 1007 1211 Size: 86 9 496 134 116 130 36 204 Cuts at: 1211 1265 1478 1659 Size: 54 213 181 Fragments arranged by size: 496 213 204 181 134 130 116 86 54 36 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 367 882 1451 1659 Size: 367 515 569 208 Fragments arranged by size: 569 515 367 208 EcoICRI GAG'CTC Cuts at: 0 981 1073 1659 Size: 981 92 586 Fragments arranged by size: 981 586 92 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 906 1089 1547 1659 Size: 906 183 458 112 Fragments arranged by size: 906 458 183 112 EcoNI CCTnn'n_nnAGG Cuts at: 0 1426 1659 Size: 1426 233 EcoO109I rG'GnC_Cy Cuts at: 0 194 1172 1659 Size: 194 978 487 Fragments arranged by size: 978 487 194 FseI GG_CCGG'CC Cuts at: 0 1379 1659 Size: 1379 280 FspI TGC'GCA Cuts at: 0 152 664 1659 Size: 152 512 995 Fragments arranged by size: 995 512 152 FspAI rTGC'GCAy Cuts at: 0 664 1659 Size: 664 995 HaeII r_GCGC'y Cuts at: 0 519 752 1048 1055 1169 1659 Size: 519 233 296 7 114 490 Fragments arranged by size: 519 490 296 233 114 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 454 486 1622 1654 1659 Size: 454 32 1136 32 5 Fragments arranged by size: 1136 454 32 32 5 KasI G'GCGC_C Cuts at: 0 1051 1165 1659 Size: 1051 114 494 Fragments arranged by size: 1051 494 114 KpnI G_GTAC'C Cuts at: 0 1422 1659 Size: 1422 237 MscI TGG'CCA Cuts at: 0 268 1659 Size: 268 1391 MslI CAynn'nnrTG Cuts at: 0 115 133 262 1659 Size: 115 18 129 1397 Fragments arranged by size: 1397 129 115 18 MspA1I CmG'CkG Cuts at: 0 91 743 757 806 976 1041 1150 1659 Size: 91 652 14 49 170 65 109 509 Fragments arranged by size: 652 509 170 109 91 65 49 14 NaeI GCC'GGC Cuts at: 0 122 1215 1377 1659 Size: 122 1093 162 282 Fragments arranged by size: 1093 282 162 122 NarI GG'CG_CC Cuts at: 0 1052 1166 1659 Size: 1052 114 493 Fragments arranged by size: 1052 493 114 NcoI C'CATG_G Cuts at: 0 263 1659 Size: 263 1396 NgoMIV G'CCGG_C Cuts at: 0 120 1213 1375 1659 Size: 120 1093 162 284 Fragments arranged by size: 1093 284 162 120 NotI GC'GGCC_GC Cuts at: 0 86 841 971 1007 1478 1659 Size: 86 755 130 36 471 181 Fragments arranged by size: 755 471 181 130 86 36 NspI r_CATG'y Cuts at: 0 483 1659 Size: 483 1176 PasI CC'CwG_GG Cuts at: 0 36 735 1659 Size: 36 699 924 Fragments arranged by size: 924 699 36 PciI A'CATG_T Cuts at: 0 479 1659 Size: 479 1180 PfoI T'CCnGG_A Cuts at: 0 668 1659 Size: 668 991 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1498 1530 1659 Size: 1498 32 129 Fragments arranged by size: 1498 129 32 PpuMI rG'GwC_Cy Cuts at: 0 1172 1659 Size: 1172 487 PspOMI G'GGCC_C Cuts at: 0 193 1659 Size: 193 1466 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 295 327 1659 Size: 295 32 1332 Fragments arranged by size: 1332 295 32 PstI C_TGCA'G Cuts at: 0 1096 1659 Size: 1096 563 RsrII CG'GwC_CG Cuts at: 0 643 1659 Size: 643 1016 SacI G_AGCT'C Cuts at: 0 983 1075 1659 Size: 983 92 584 Fragments arranged by size: 983 584 92 SacII CC_GC'GG Cuts at: 0 92 758 977 1151 1659 Size: 92 666 219 174 508 Fragments arranged by size: 666 508 219 174 92 SfcI C'TryA_G Cuts at: 0 240 1080 1092 1647 1659 Size: 240 840 12 555 12 Fragments arranged by size: 840 555 240 12 12 SfiI GGCCn_nnn'nGGCC Cuts at: 0 94 1006 1659 Size: 94 912 653 Fragments arranged by size: 912 653 94 SfoI GGC'GCC Cuts at: 0 1053 1167 1659 Size: 1053 114 492 Fragments arranged by size: 1053 492 114 SmaI CCC'GGG Cuts at: 0 170 821 1248 1493 1659 Size: 170 651 427 245 166 Fragments arranged by size: 651 427 245 170 166 SmlI C'TyrA_G Cuts at: 0 571 1659 Size: 571 1088 StyI C'CwwG_G Cuts at: 0 218 263 1398 1659 Size: 218 45 1135 261 Fragments arranged by size: 1135 261 218 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 697 1659 Size: 697 962 TatI w'GTAC_w Cuts at: 0 137 1659 Size: 137 1522 XmaI C'CCGG_G Cuts at: 0 168 819 1246 1491 1659 Size: 168 651 427 245 168 Fragments arranged by size: 651 427 245 168 168 ZraI GAC'GTC Cuts at: 0 944 1659 Size: 944 715 Enzymes that do cut: AatII AccI Acc65I AcuI AflIII AlfI AlwNI ApaI AscI AvaI BanI BanII BbeI BglI BlpI Bme1580I BmgBI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstEII BstXI BtgI BtgZI BtsI DraIII EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI FspI FspAI HaeII Hin4I KasI KpnI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PciI PfoI PpiI PpuMI PspOMI PsrI PstI RsrII SacI SacII SfcI SfiI SfoI SmaI SmlI StyI TaqII TatI XmaI ZraI Enzymes that do not cut: AarI AclI AfeI AflII AgeI AhdI AleI AloI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmrI BmtI Bpu10I BsaBI BsiWI BsmI BsmBI BsrDI BsrGI BstAPI BstBI BstYI BstZ17I Bsu36I ClaI CspCI DraI DrdI EarI EcoRI EcoRV FalI HincII HindIII HpaI MfeI MluI MmeI NdeI NheI NruI NsiI PacI PflMI PmeI PmlI PshAI PsiI PspXI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmnI