################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AvrII BamHI BbrPI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraIII EarI Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI HphI KpnI Ksp632I MaeII MamI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AhdI 79 GACGCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 323 GTGCAC G^TGCAC AocI 855 CCTCAGG CC^T[ACGT]AGG ApaI 953 GGGCCC GGGCC^C ApaLI 323 GTGCAC G^TGCAC AspEI 79 GACGCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 323 GTGCAC G[AT]GC[AT]^C AspI 616 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 663 GGTCC G^G[AT]CC AviII 830 TGCGCA TGC^GCA BalI 389 TGGCCA TGG^CCA BbsI 622 GTCTTC GAAGAC(2/6) BbvCI 645 CCTCAGC CCTCAGC(-5/-2) BcgI 192 CGAACAGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 1204 CTAG C^TAG BpuAI 622 GTCTTC GAAGAC(2/6) BsgI 135 CTGCAC GTGCAG(16/14) BsiHKAI 323 GTGCAC G[AT]GC[AT]^C BsiWI 974 CGTACG C^GTACG BspMI 1178 ACCTGC ACCTGC(4/8) BsrI 578 ACTGG ACTGG(1/-1) BstYI 174 AGATCC [GA]^GATC[CT] Bsu36I 855 CCTCAGG CC^T[ACGT]AGG DrdI 523 GACTGCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 79 GACGCGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 855 CCTCAGG CC^T[ACGT]AGG EcoT14I 386 CCATGG C^C[AT][AT]GG FspI 830 TGCGCA TGC^GCA MaeI 1204 CTAG C^TAG MaeIII 53 GTGAC ^GT[ACGT]AC MboII 623 TCTTC GAAGA(8/7) MflI 174 AGATCC [GA]^GATC[CT] MluNI 389 TGGCCA TGG^CCA MscI 389 TGGCCA TGG^CCA MslI 381 CATCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 386 CCATGG C^CATGG NspI 749 GCATGC [GA]CATG^[CT] PflFI 616 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 242 CAGCTG CAG^CTG SphI 749 GCATGC GCATG^C StyI 386 CCATGG C^C[AT][AT]GG TaqI 818 TCGA T^CGA Tsp45I 53 GTGAC ^GT[GC]AC Tth111I 616 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 818 TCGA T^CGA XhoII 174 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwI 175 GATCC GGATC(4/5) 231 GATCC AlwNI 239 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 242 CAGCTGCTG BanII 120 GGGCTC G[GA]GC[CT]^C 953 GGGCCC BpmI 801 CTCCAG CTGGAG(16/14) 58 CTGGAG BsmAI 107 GAGAC GTCTC(1/5) 1195 GAGAC BsmBI 107 GAGACG CGTCTC(1/5) 1195 GAGACG BstAPI 238 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 241 GCAGCTGCTGC EagI 152 CGGCCG C^GGCCG 1097 CGGCCG EclXI 152 CGGCCG C^GGCCG 1097 CGGCCG Eco52I 152 CGGCCG C^GGCCG 1097 CGGCCG HinfI 112 GACTC G^A[ACGT]TC 400 GACTC NaeI 990 GCCGGC GCC^GGC 1099 GCCGGC NgoMIV 990 GCCGGC G^CCGGC 1099 GCCGGC PleI 112 GACTC GAGTC(4/5) 400 GACTC SbfI 947 CCTGCAGG CCTGCA^GG 1143 CCTGCAGG SfaNI 582 GATGC GCATC(5/9) 824 GCATC Sse8387I 947 CCTGCAGG CCTGCA^GG 1143 CCTGCAGG TspRI 51 CAGTG CA[GC]TG[ACGT][ACGT]^ 886 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AvaI BanI BbeI BbvI BcnI BglI BmyI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BsrBI BsrFI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI KasI KspI MboI MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PstI RsaI SacII Sau3AI Sau96I ScrFI SfcI SfoI SmaI TseI XmaCI XmaI