(Circular) (Six-base) MAPSORT of: pFN21AB9605.seq Check: 285 from: 1 to: 5914 pFN21AB9605 5914 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3192 3192 Size: 5914 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3724 278 Size: 53 83 186 936 2188 2468 Fragments arranged by size: 2468 2188 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3257 1937 Size: 1320 4594 Acc65I G'GTAC_C Cuts at: 3235 3235 Size: 5914 AclI AA'CG_TT Cuts at: 1563 4041 4414 1563 Size: 2478 373 3063 Fragments arranged by size: 3063 2478 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2873 3977 5361 5629 1632 Size: 1241 1104 1384 268 1917 Fragments arranged by size: 1917 1384 1241 1104 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5717 AflIII A'CryG_T Cuts at: 1203 4813 5790 1203 Size: 3610 977 1327 Fragments arranged by size: 3610 1327 977 AgeI A'CCGG_T Cuts at: 4745 4745 Size: 5914 AhdI GACnn_n'nnGTC Cuts at: 2089 4643 2089 Size: 2554 3360 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5914 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2519 2978 1184 Size: 1335 459 4120 Fragments arranged by size: 4120 1335 459 AlwNI CAG_nnn'CTG Cuts at: 2249 2252 3657 5229 2249 Size: 3 1405 1572 2934 Fragments arranged by size: 2934 1572 1405 3 ApaI G_GGCC'C Cuts at: 2962 2962 Size: 5914 ApaLI G'TGCA_C Cuts at: 2328 3971 5127 2328 Size: 1643 1156 3115 Fragments arranged by size: 3115 1643 1156 ApoI r'AATT_y Cuts at: 1493 3223 3449 3601 5710 1493 Size: 1730 226 152 2109 1697 Fragments arranged by size: 2109 1730 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4468 160 Size: 872 3436 1606 Fragments arranged by size: 3436 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5914 AvaI C'yCGr_G Cuts at: 1870 1943 2678 2690 2701 2876 3239 1870 Size: 73 735 12 11 175 363 4545 Fragments arranged by size: 4545 735 363 175 73 12 11 BamHI G'GATC_C Cuts at: 3244 3611 3244 Size: 367 5547 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2171 2897 2947 3025 3235 Size: 325 217 627 384 726 50 78 210 Cuts at: 3235 4690 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 726 627 384 325 217 210 78 50 BanII G_rGCy'C Cuts at: 1391 2129 2962 3233 1391 Size: 738 833 271 4072 Fragments arranged by size: 4072 833 738 271 BbeI G_GCGC'C Cuts at: 2175 2901 2951 2175 Size: 726 50 5138 Fragments arranged by size: 5138 726 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2620 928 Size: 971 721 4222 Fragments arranged by size: 4222 971 721 BbvCI CC'TCA_GC Cuts at: 2651 2651 Size: 5914 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2186 2220 4105 4139 1903 Size: 34 249 34 1885 34 3678 Fragments arranged by size: 3678 1885 249 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3806 5016 3806 Size: 1210 4704 BclI T'GATC_A Cuts at: 1548 1548 Size: 5914 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2288 2741 2796 Size: 107 122 71 631 605 616 453 55 Cuts at: 2796 3001 3152 3174 4525 136 Size: 205 151 22 1351 1525 Fragments arranged by size: 1525 1351 631 616 605 453 205 151 122 107 71 55 22 BglII A'GATC_T Cuts at: 5909 5909 Size: 5914 BlpI GC'TnA_GC Cuts at: 3331 3331 Size: 5914 Bme1580I G_kGCm'C Cuts at: 1790 2332 2962 3975 5131 1790 Size: 542 630 1013 1156 2573 Fragments arranged by size: 2573 1156 1013 630 542 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5914 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3645 4603 442 Size: 416 53 83 399 24 2228 958 1753 Fragments arranged by size: 2228 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5914 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 2791 4574 1239 Size: 845 707 1783 2579 Fragments arranged by size: 2579 1783 845 707 Bpu10I CC'TnA_GC Cuts at: 2651 2651 Size: 5914 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4039 4904 5202 4039 Size: 865 298 4751 Fragments arranged by size: 4751 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4577 882 Size: 617 3078 2219 Fragments arranged by size: 3078 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3705 493 Size: 711 2501 2702 Fragments arranged by size: 2702 2501 711 BsaBI GATnn'nnATC Cuts at: 5908 5908 Size: 5914 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2172 2898 2948 3721 Size: 53 83 186 936 639 726 50 773 Cuts at: 3721 4103 4738 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 773 726 639 635 382 186 83 53 50 BsaWI w'CCGG_w Cuts at: 4347 4745 5019 5166 5577 4347 Size: 398 274 147 411 4684 Fragments arranged by size: 4684 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5689 Fragments arranged by size: 5689 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2243 2708 2792 2927 1186 Size: 1057 465 84 135 4173 Fragments arranged by size: 4173 1057 465 135 84 BseYI C'CCAG_C Cuts at: 3004 5117 5613 3004 Size: 2113 496 3305 Fragments arranged by size: 3305 2113 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2125 1163 Size: 573 389 4952 Fragments arranged by size: 4952 573 389 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2160 3036 3105 3364 4125 5153 Size: 275 186 159 876 69 259 761 1028 Cuts at: 5153 1540 Size: 2301 Fragments arranged by size: 2301 1028 876 761 275 259 186 159 69 BsiHKAI G_wGCw'C Cuts at: 2332 3975 4060 5131 2332 Size: 1643 85 1071 3115 Fragments arranged by size: 3115 1643 1071 85 BsiWI C'GTAC_G Cuts at: 2979 2979 Size: 5914 BsmI GAATG_Cn' Cuts at: 3425 3518 3425 Size: 93 5821 BsmBI CGTCTCn'nnnn_ Cuts at: 2106 3194 2106 Size: 1088 4826 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2129 2332 2962 3233 3975 4060 5131 Size: 399 339 203 630 271 742 85 1071 Cuts at: 5131 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 630 399 339 271 203 85 BspEI T'CCGG_A Cuts at: 5577 5577 Size: 5914 BspHI T'CATG_A Cuts at: 3803 5533 3803 Size: 1730 4184 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3192 3269 844 Size: 2348 77 3489 Fragments arranged by size: 3489 2348 77 BsrBI CCG'CTC Cuts at: 1394 1823 2319 2971 3046 3801 1394 Size: 429 496 652 75 755 3507 Fragments arranged by size: 3507 755 652 496 429 75 BsrDI GCAATG_nn' Cuts at: 65 1248 4409 4583 65 Size: 1183 3161 174 1396 Fragments arranged by size: 3161 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2665 2995 3104 4558 4745 1700 Size: 110 855 330 109 1454 187 2869 Fragments arranged by size: 2869 1454 855 330 187 110 109 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5914 BssHII G'CGCG_C Cuts at: 1925 2661 2782 3009 3011 3111 1925 Size: 736 121 227 2 100 4728 Fragments arranged by size: 4728 736 227 121 100 2 BssSI C'ACGA_G Cuts at: 3974 4986 3974 Size: 1012 4902 BstAPI GCAn_nnn'nTGC Cuts at: 794 2249 2252 794 Size: 1455 3 4456 Fragments arranged by size: 4456 1455 3 BstBI TT'CG_AA Cuts at: 4718 4718 Size: 5914 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5914 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5914 BstYI r'GATC_y Cuts at: 1972 2179 3244 3611 3996 4013 5465 5551 5563 Size: 207 1065 367 385 17 1452 86 12 Cuts at: 5563 5909 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1065 385 367 346 207 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2861 2861 Size: 5914 BtgI C'CryG_G Cuts at: 513 1065 2038 2160 2391 2473 2793 2943 513 Size: 552 973 122 231 82 320 150 3484 Fragments arranged by size: 3484 973 552 320 231 150 122 82 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2362 2371 487 Size: 683 56 1136 9 4030 Fragments arranged by size: 4030 1136 683 56 9 BtsI GCAGTG_nn' Cuts at: 2062 3430 4224 4244 2062 Size: 1368 794 20 3732 Fragments arranged by size: 3732 1368 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5914 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5879 DraI TTT'AAA Cuts at: 3218 3218 Size: 5914 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2534 4921 809 Size: 507 1218 2387 1802 Fragments arranged by size: 2387 1802 1218 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2145 2157 2394 Size: 54 1425 50 275 18 315 12 237 Cuts at: 2394 3102 3361 4250 5879 8 Size: 708 259 889 1629 43 Fragments arranged by size: 1629 1425 889 708 315 275 259 237 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2157 3102 3361 1537 Size: 275 345 945 259 4090 Fragments arranged by size: 4090 945 345 275 259 EarI CTCTTCn'nnn_ Cuts at: 1353 3844 1353 Size: 2491 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2172 2282 2447 2621 3155 4501 Size: 1064 138 583 110 165 174 534 1346 Cuts at: 4501 4875 5021 387 Size: 374 146 1280 Fragments arranged by size: 1346 1280 1064 583 534 374 174 165 146 138 110 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2791 2873 3977 4574 5361 5629 Size: 393 452 707 82 1104 597 787 268 Cuts at: 5629 1239 Size: 1524 Fragments arranged by size: 1524 1104 787 707 597 452 393 268 82 EcoO109I rG'GnC_Cy Cuts at: 2698 2958 2959 2698 Size: 260 1 5653 Fragments arranged by size: 5653 260 1 EcoRI G'AATT_C Cuts at: 3223 3601 3223 Size: 378 5536 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2482 2482 Size: 5914 FspI TGC'GCA Cuts at: 2837 4420 2837 Size: 1583 4331 HaeII r_GCGC'y Cuts at: 1678 2175 2422 2650 2901 2951 5061 1678 Size: 497 247 228 251 50 2110 2531 Fragments arranged by size: 2531 2110 497 251 247 228 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2075 2107 4555 4587 4629 Size: 32 135 32 503 32 2448 32 42 Cuts at: 4629 4661 1373 Size: 32 2626 Fragments arranged by size: 2626 2448 503 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3258 3504 669 Size: 988 281 1320 246 3079 Fragments arranged by size: 3079 1320 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5914 HpaI GTT'AAC Cuts at: 3504 3504 Size: 5914 KasI G'GCGC_C Cuts at: 2171 2897 2947 2171 Size: 726 50 5138 Fragments arranged by size: 5138 726 50 KpnI G_GTAC'C Cuts at: 3239 3239 Size: 5914 MfeI C'AATT_G Cuts at: 3513 3513 Size: 5914 MluI A'CGCG_T Cuts at: 5790 5790 Size: 5914 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1992 5028 5212 1305 Size: 687 3036 184 2007 Fragments arranged by size: 3036 2007 687 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2396 10 Size: 54 1425 343 564 3528 Fragments arranged by size: 3528 1425 564 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2390 3872 4231 4390 518 Size: 648 1224 1482 359 159 2042 Fragments arranged by size: 2042 1482 1224 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2098 2162 2249 2258 2475 2795 2917 2945 Size: 492 64 87 9 217 320 122 28 Cuts at: 2945 3330 4007 5155 5400 1606 Size: 385 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 492 385 320 245 217 122 87 64 28 9 NaeI GCC'GGC Cuts at: 2997 3106 2997 Size: 109 5805 NarI GG'CG_CC Cuts at: 2172 2898 2948 2172 Size: 726 50 5138 Fragments arranged by size: 5138 726 50 NcoI C'CATG_G Cuts at: 513 1065 2391 513 Size: 552 1326 4036 Fragments arranged by size: 4036 1326 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5914 NgoMIV G'CCGG_C Cuts at: 2995 3104 2995 Size: 109 5805 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5914 NotI GC'GGCC_GC Cuts at: 3361 3361 Size: 5914 NspI r_CATG'y Cuts at: 1132 1229 2758 3272 4817 1132 Size: 97 1529 514 1545 2229 Fragments arranged by size: 2229 1545 1529 514 97 PasI CC'CwG_GG Cuts at: 1348 2122 2565 1348 Size: 774 443 4697 Fragments arranged by size: 4697 774 443 PciI A'CATG_T Cuts at: 4813 4813 Size: 5914 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5914 PmeI GTTT'AAAC Cuts at: 3218 3218 Size: 5914 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3983 4015 5496 5528 3983 Size: 32 1481 32 4369 Fragments arranged by size: 4369 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5914 PsiI TTA'TAA Cuts at: 3484 3484 Size: 5914 PspOMI G'GGCC_C Cuts at: 2958 2958 Size: 5914 PstI C_TGCA'G Cuts at: 830 2257 2957 3153 3266 830 Size: 1427 700 196 113 3478 Fragments arranged by size: 3478 1427 700 196 113 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5914 PvuII CAG'CTG Cuts at: 2249 2249 Size: 5914 SacII CC_GC'GG Cuts at: 2163 2476 2796 2946 2163 Size: 313 320 150 5131 Fragments arranged by size: 5131 320 313 150 SalI G'TCGA_C Cuts at: 1936 3256 1936 Size: 1320 4594 SbfI CC_TGCA'GG Cuts at: 2957 3153 3266 2957 Size: 196 113 5605 Fragments arranged by size: 5605 196 113 ScaI AGT'ACT Cuts at: 4162 5874 4162 Size: 1712 4202 SfcI C'TryA_G Cuts at: 826 1045 2253 2953 3149 3262 4397 5078 5269 Size: 219 1208 700 196 113 1135 681 191 Cuts at: 5269 826 Size: 1471 Fragments arranged by size: 1471 1208 1135 700 681 219 196 191 113 SfoI GGC'GCC Cuts at: 2173 2899 2949 2173 Size: 726 50 5138 Fragments arranged by size: 5138 726 50 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5914 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5914 SmaI CCC'GGG Cuts at: 1872 2680 2703 2878 3241 1872 Size: 808 23 175 363 4545 Fragments arranged by size: 4545 808 363 175 23 SmlI C'TyrA_G Cuts at: 820 1017 1943 4018 4919 5181 820 Size: 197 926 2075 901 262 1553 Fragments arranged by size: 2075 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3705 493 Size: 3212 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5914 SphI G_CATG'C Cuts at: 1132 2758 3272 1132 Size: 1626 514 3774 Fragments arranged by size: 3774 1626 514 SrfI GCCC'GGGC Cuts at: 2680 2680 Size: 5914 SspI AAT'ATT Cuts at: 5 52 3838 5 Size: 47 3786 2081 Fragments arranged by size: 3786 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5914 StyI C'CwwG_G Cuts at: 513 1065 2391 3353 513 Size: 552 1326 962 3074 Fragments arranged by size: 3074 1326 962 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3941 3958 4111 4296 4741 325 Size: 1255 2361 17 153 185 445 1498 Fragments arranged by size: 2361 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2607 3120 4160 Size: 274 80 33 51 1443 630 513 1040 Cuts at: 4160 5872 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1040 630 513 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5874 5906 5874 Size: 32 5882 Tth111I GACn'n_nGTC Cuts at: 1316 2624 1316 Size: 1308 4606 XbaI T'CTAG_A Cuts at: 3250 3250 Size: 5914 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5914 XmaI C'CCGG_G Cuts at: 1870 2678 2701 2876 3239 1870 Size: 808 23 175 363 4545 Fragments arranged by size: 4545 808 363 175 23 XmnI GAAnn'nnTTC Cuts at: 4043 4043 Size: 5914 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3722 276 Size: 53 83 186 936 2188 2468 Fragments arranged by size: 2468 2188 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SwaI XcmI