Restriction map for
pFN21AE6689
##################################
# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI AlwNI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaII
AviII AvrII BalI BamHI BanII
BbeI BbsI BcgI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
Hin1I HincII HindII HinfI HpaI
KasI KpnI MamI MfeI MluI
MluNI MroI MscI MslI MunI
NaeI NarI NcoI NdeI NgoMIV
NotI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI StuI
StyI SwaI TfiI Tsp509I Tth111I
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Alw44I 385 GTGCAC G^TGCAC
AlwI 231 GATCC GGATC(4/5)
ApaLI 385 GTGCAC G^TGCAC
AvaI 263 CCCGGG C^[CT]CG[GA]G
BanI 61 GGCACC G^G[CT][GA]CC
BbrPI 532 CACGTG CAC^GTG
BbvCI 492 GCTGAGG CCTCAGC(-5/-2)
BglII 219 AGATCT A^GATCT
BsaAI 532 CACGTG [CT]AC^GT[GA]
BseRI 495 GAGGAG GAGGAG(10/8)
BsmBI 580 GAGACG CGTCTC(1/5)
BsoBI 263 CCCGGG C^[CT]CG[GA]G
BsrFI 22 ACCGGC [GA]^CCGG[CT]
BsrI 633 CCAGT ACTGG(1/-1)
BssHII 450 GCGCGC G^CGCGC
BssSI 148 CACGAG CACGAG(-5/-1)
BstAPI 387 GCACGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 22 ACCGGC [GA]^CCGG[CT]
DraI 610 TTTAAA TTT^AAA
DraII 181 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 128 CCGCGG C^C[GA][CT]GG
EcoNI 144 CCTCCACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 181 GGGGCCT [GA]G^G[ACGT]CC[CT]
HaeII 320 AGCGCC [GA]GCGC^[CT]
HindIII 703 AAGCTT A^AGCTT
HphI 20 TCACC GGTGA(8/7)
KspI 128 CCGCGG CCGC^GG
MaeIII 530 GTCAC ^GT[ACGT]AC
MseI 611 TTAA T^TAA
NheI 75 GCTAGC G^CTAGC
NruI 68 TCGCGA TCG^CGA
PflMI 155 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 532 CACGTG CAC^GTG
PmlI 532 CACGTG CAC^GTG
PvuII 307 CAGCTG CAG^CTG
SacII 128 CCGCGG CCGC^GG
SmaI 263 CCCGGG CCC^GGG
SspI 757 AATATT AAT^ATT
TaqI 224 TCGA T^CGA
Tru9I 611 TTAA T^TAA
Tsp45I 530 GTCAC ^GT[GC]AC
TspRI 555 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 224 TCGA T^CGA
Van91I 155 CCAGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 36 TCTAGA T^CTAGA
XmaCI 263 CCCGGG C^CCGGG
XmaI 263 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BcnI 263 CCCGG CC^[GC]GG
264 CCGGG
BfaI 37 CTAG C^TAG
76 CTAG
BsaI 662 GAGACC GGTCTC(1/5)
354 GGTCTC
BstYI 219 AGATCT [GA]^GATC[CT]
230 AGATCC
EarI 773 CTCTTC CTCTTC(1/4)
809 CTCTTC
Ksp632I 773 CTCTTC CTCTTC(1/4)
809 CTCTTC
MaeI 37 CTAG C^TAG
76 CTAG
MflI 219 AGATCT [GA]^GATC[CT]
230 AGATCC
NciI 263 CCCGG CC^[GC]GG
264 CCGGG
NlaIII 201 CATG CATG^
675 CATG
PstI 304 CTGCAG CTGCA^G
709 CTGCAG
SfaNI 467 GCATC GCATC(5/9)
500 GCATC
SfcI 304 CTGCAG C^T[GA][CT]AG
709 CTGCAG
XhoII 219 AGATCT [GA]^GATC[CT]
230 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AccII AciI AluI AspHI BbvI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HpaII ItaI MaeII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIV
Sau3AI Sau96I ScrFI TseI