Restriction map for
pFN21AE6689
(Circular) (Six-base) MAPSORT of: pFN21AE6689.seq Check: 4169 from: 1 to: 5539
pFN21AE6689 5539 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3349 278
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2882 1937
Size: 945 4594
Acc65I G'GTAC_C
Cuts at: 2860 2860
Size: 5539
AclI AA'CG_TT
Cuts at: 1563 3666 4039 1563
Size: 2103 373 3063
Fragments arranged by size:
3063 2103 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2324 2768 2801 3602 4986 5254 1632
Size: 692 444 33 801 1384 268 1917
Fragments arranged by size:
1917 1384 801 692 444 268 33
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5342
AflIII A'CryG_T
Cuts at: 1203 4438 5415 1203
Size: 3235 977 1327
Fragments arranged by size:
3235 1327 977
AgeI A'CCGG_T
Cuts at: 4370 4370
Size: 5539
AhdI GACnn_n'nnGTC
Cuts at: 4268 4268
Size: 5539
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5539
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2405 2495 2555 1184
Size: 1221 90 60 4168
Fragments arranged by size:
4168 1221 90 60
AlwNI CAG_nnn'CTG
Cuts at: 3282 4854 3282
Size: 1572 3967
ApaLI G'TGCA_C
Cuts at: 2390 3596 4752 2390
Size: 1206 1156 3177
Fragments arranged by size:
3177 1206 1156
ApoI r'AATT_y
Cuts at: 1493 2848 3074 3226 5335 1493
Size: 1355 226 152 2109 1697
Fragments arranged by size:
2109 1697 1355 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4093 160
Size: 872 3061 1606
Fragments arranged by size:
3061 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
AvaI C'yCGr_G
Cuts at: 1870 1943 2268 2864 1870
Size: 73 325 596 4545
Fragments arranged by size:
4545 596 325 73
BamHI G'GATC_C
Cuts at: 2869 3236 2869
Size: 367 5172
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 2860 4315 618
Size: 325 217 627 279 794 1455 1842
Fragments arranged by size:
1842 1455 794 627 325 279 217
BanII G_rGCy'C
Cuts at: 1391 2858 1391
Size: 1467 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4568
BbvCI CC'TCA_GC
Cuts at: 2498 2498
Size: 5539
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3730 3764 1903
Size: 34 1793 34 3678
Fragments arranged by size:
3678 1793 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3431 4641 3431
Size: 1210 4329
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5539
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4150 136
Size: 107 122 71 631 605 2478 1525
Fragments arranged by size:
2478 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2224 5534 2224
Size: 3310 2229
BlpI GC'TnA_GC
Cuts at: 2956 2956
Size: 5539
Bme1580I G_kGCm'C
Cuts at: 1790 2394 3600 4756 1790
Size: 604 1206 1156 2573
Fragments arranged by size:
2573 1206 1156 604
BmgBI CAC'GTC
Cuts at: 1318 2059 1318
Size: 741 4798
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2632 3270 4228
Size: 416 53 83 399 24 1215 638 958
Cuts at: 4228 442
Size: 1753
Fragments arranged by size:
1753 1215 958 638 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2084 1056
Size: 1028 4511
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 5539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2264 2509 2693 4199 1239
Size: 1025 245 184 1506 2579
Fragments arranged by size:
2579 1506 1025 245 184
Bpu10I CC'TnA_GC
Cuts at: 2498 2498
Size: 5539
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3664 4529 4827 3664
Size: 865 298 4376
Fragments arranged by size:
4376 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2365 2661 4202 882
Size: 617 866 296 1541 2219
Fragments arranged by size:
2219 1541 866 617 296
BsaAI yAC'GTr
Cuts at: 493 1204 2539 3330 493
Size: 711 1335 791 2702
Fragments arranged by size:
2702 1335 791 711
BsaBI GATnn'nnATC
Cuts at: 5533 5533
Size: 5539
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3346 3728 4363 275
Size: 53 83 186 936 1813 382 635 1451
Fragments arranged by size:
1813 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3972 4370 4644 4791 5202 3972
Size: 398 274 147 411 4309
Fragments arranged by size:
4309 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2249 2279 2582 2612 1178
Size: 30 165 30 846 30 303 30 4105
Fragments arranged by size:
4105 846 303 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2515 1186
Size: 1329 4210
BseYI C'CCAG_C
Cuts at: 2447 4742 5238 2447
Size: 2295 496 2748
Fragments arranged by size:
2748 2295 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4966
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2989 3750 4778 1540
Size: 275 186 988 761 1028 2301
Fragments arranged by size:
2301 1028 988 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2100 2262 2394 3600 3685 4756 2100
Size: 162 132 1206 85 1071 2883
Fragments arranged by size:
2883 1206 1071 162 132 85
BsmI GAATG_Cn'
Cuts at: 3050 3143 3050
Size: 93 5446
BsmBI CGTCTCn'nnnn_
Cuts at: 2579 2579
Size: 5539
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2262 2394 2858 3600 3685 4756
Size: 399 310 162 132 464 742 85 1071
Cuts at: 4756 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 464 399 310 162 132
85
BspEI T'CCGG_A
Cuts at: 5202 5202
Size: 5539
BspHI T'CATG_A
Cuts at: 3428 5158 3428
Size: 1730 3809
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2894 844
Size: 2050 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3426 1394
Size: 429 1603 3507
Fragments arranged by size:
3507 1603 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4034 4208 65
Size: 1183 2786 174 1396
Fragments arranged by size:
2786 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2027 4183 4370 1700
Size: 110 217 2156 187 2869
Fragments arranged by size:
2869 2156 217 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5539
BssHII G'CGCG_C
Cuts at: 1925 2455 1925
Size: 530 5009
BssSI C'ACGA_G
Cuts at: 2153 3599 4611 2153
Size: 1446 1012 3081
Fragments arranged by size:
3081 1446 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2398 794
Size: 1604 3935
BstBI TT'CG_AA
Cuts at: 4343 4343
Size: 5539
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5539
BstYI r'GATC_y
Cuts at: 1972 2224 2235 2869 3236 3621 3638 5090 5176
Size: 252 11 634 367 385 17 1452 86
Cuts at: 5176 5188 5534 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 634 385 367 346 252 86
17 12 11
BtgI C'CryG_G
Cuts at: 513 1065 2133 513
Size: 552 1068 3919
Fragments arranged by size:
3919 1068 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4800
Fragments arranged by size:
4800 683 56
BtsI GCAGTG_nn'
Cuts at: 3055 3849 3869 3055
Size: 794 20 4725
Fragments arranged by size:
4725 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5539
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5504
DraI TTT'AAA
Cuts at: 2617 2843 2617
Size: 226 5313
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4546 809
Size: 507 3230 1802
Fragments arranged by size:
3230 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2986 3875 5504
Size: 54 1425 50 275 18 1156 889 1629
Cuts at: 5504 8
Size: 43
Fragments arranged by size:
1629 1425 1156 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2986 1537
Size: 275 1174 4090
Fragments arranged by size:
4090 1174 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2784 2820 3469 1353
Size: 1431 36 649 3423
Fragments arranged by size:
3423 1431 649 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2270 2465 4126 4500 4646 387
Size: 1064 138 681 195 1661 374 146 1280
Fragments arranged by size:
1661 1280 1064 681 374 195 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2264 2324 2509 2693 2768 2801 3602
Size: 393 632 60 185 184 75 33 801
Cuts at: 3602 4199 4986 5254 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 801 787 632 597 393 268 185
184 75 60 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2153 2153
Size: 5539
EcoO109I rG'GnC_Cy
Cuts at: 2187 2187
Size: 5539
EcoRI G'AATT_C
Cuts at: 2848 3226 2848
Size: 378 5161
FspI TGC'GCA
Cuts at: 4045 4045
Size: 5539
HaeII r_GCGC'y
Cuts at: 1678 2329 4686 1678
Size: 651 2357 2531
Fragments arranged by size:
2531 2357 651
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2249 2281 2582 2614 4180
Size: 32 135 32 677 32 301 32 1566
Cuts at: 4180 4212 4254 4286 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1566 677 301 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2883 3129 669
Size: 988 281 945 246 3079
Fragments arranged by size:
3079 988 945 281 246
HindIII A'AGCT_T
Cuts at: 748 2708 748
Size: 1960 3579
HpaI GTT'AAC
Cuts at: 3129 3129
Size: 5539
KpnI G_GTAC'C
Cuts at: 2864 2864
Size: 5539
MfeI C'AATT_G
Cuts at: 3138 3138
Size: 5539
MluI A'CGCG_T
Cuts at: 5415 5415
Size: 5539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2487 4653 4837 1305
Size: 1182 2166 184 2007
Fragments arranged by size:
2166 2007 1182 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3717
Fragments arranged by size:
3717 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3497 3856 4015 518
Size: 648 2331 359 159 2042
Fragments arranged by size:
2331 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2064 2135 2288 2314 2955 3632 4780 5025
Size: 458 71 153 26 641 677 1148 245
Cuts at: 5025 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 641 458 245 153 71
26
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4987
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5539
NheI G'CTAG_C
Cuts at: 1052 2080 1052
Size: 1028 4511
NotI GC'GGCC_GC
Cuts at: 2986 2986
Size: 5539
NruI TCG'CGA
Cuts at: 2075 2075
Size: 5539
NspI r_CATG'y
Cuts at: 1132 1229 2897 4442 1132
Size: 97 1668 1545 2229
Fragments arranged by size:
2229 1668 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2368 1348
Size: 1020 4519
PciI A'CATG_T
Cuts at: 4438 4438
Size: 5539
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2166 1108
Size: 1058 4481
PmeI GTTT'AAAC
Cuts at: 2843 2843
Size: 5539
PmlI CAC'GTG
Cuts at: 2539 2539
Size: 5539
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3608 3640 5121 5153 3608
Size: 32 1481 32 3994
Fragments arranged by size:
3994 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5539
PsiI TTA'TAA
Cuts at: 3109 3109
Size: 5539
PstI C_TGCA'G
Cuts at: 830 2313 2718 2891 830
Size: 1483 405 173 3478
Fragments arranged by size:
3478 1483 405 173
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5539
PvuII CAG'CTG
Cuts at: 2314 2314
Size: 5539
SacII CC_GC'GG
Cuts at: 2136 2136
Size: 5539
SalI G'TCGA_C
Cuts at: 1936 2881 1936
Size: 945 4594
SbfI CC_TGCA'GG
Cuts at: 2891 2891
Size: 5539
ScaI AGT'ACT
Cuts at: 3787 5499 3787
Size: 1712 3827
SfcI C'TryA_G
Cuts at: 826 1045 2309 2714 2887 4022 4703 4894 826
Size: 219 1264 405 173 1135 681 191 1471
Fragments arranged by size:
1471 1264 1135 681 405 219 191 173
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5539
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5539
SmaI CCC'GGG
Cuts at: 1872 2270 2866 1872
Size: 398 596 4545
Fragments arranged by size:
4545 596 398
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3643 4544 4806 820
Size: 197 926 1700 901 262 1553
Fragments arranged by size:
1700 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3330 493
Size: 2837 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5539
SphI G_CATG'C
Cuts at: 1132 2897 1132
Size: 1765 3774
SspI AAT'ATT
Cuts at: 5 52 2764 3463 5
Size: 47 2712 699 2081
Fragments arranged by size:
2712 2081 699 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5539
StyI C'CwwG_G
Cuts at: 513 1065 2835 2978 513
Size: 552 1770 143 3074
Fragments arranged by size:
3074 1770 552 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2639 3566 3583 3736 3921 4366 325
Size: 1255 1059 927 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1059 927 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3785 5497 96
Size: 274 80 33 51 1443 1808 1712 138
Fragments arranged by size:
1808 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5499 5531 5499
Size: 32 5507
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5539
XbaI T'CTAG_A
Cuts at: 2041 2875 2041
Size: 834 4705
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5539
XmaI C'CCGG_G
Cuts at: 1870 2268 2864 1870
Size: 398 596 4545
Fragments arranged by size:
4545 596 398
XmnI GAAnn'nnTTC
Cuts at: 3668 3668
Size: 5539
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3347 276
Size: 53 83 186 936 1813 2468
Fragments arranged by size:
2468 1813 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NruI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI
PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI