(Circular) (Six-base) MAPSORT of: pFN21AE6018.seq Check: 6301 from: 1 to: 5650 pFN21AE6018 5650 bp With 182 enzymes: SgfI * December 8, 2014 13:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2735 2735 Size: 5650 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3460 278 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2577 2993 1937 Size: 640 416 4594 Fragments arranged by size: 4594 640 416 Acc65I G'GTAC_C Cuts at: 2971 2971 Size: 5650 AclI AA'CG_TT Cuts at: 1563 3777 4150 1563 Size: 2214 373 3063 Fragments arranged by size: 3063 2214 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3713 5097 5365 1632 Size: 2081 1384 268 1917 Fragments arranged by size: 2081 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5453 AflIII A'CryG_T Cuts at: 1203 4549 5526 1203 Size: 3346 977 1327 Fragments arranged by size: 3346 1327 977 AgeI A'CCGG_T Cuts at: 4481 4481 Size: 5650 AhdI GACnn_n'nnGTC Cuts at: 4379 4379 Size: 5650 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5650 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5650 AlwNI CAG_nnn'CTG Cuts at: 3393 4965 3393 Size: 1572 4078 ApaI G_GGCC'C Cuts at: 2809 2809 Size: 5650 ApaLI G'TGCA_C Cuts at: 3707 4863 3707 Size: 1156 4494 ApoI r'AATT_y Cuts at: 1493 2500 2959 3185 3337 5446 1493 Size: 1007 459 226 152 2109 1697 Fragments arranged by size: 2109 1697 1007 459 226 152 AseI AT'TA_AT Cuts at: 160 1032 4204 160 Size: 872 3172 1606 Fragments arranged by size: 3172 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 AvaI C'yCGr_G Cuts at: 1870 1943 2975 1870 Size: 73 1032 4545 Fragments arranged by size: 4545 1032 73 AvrII C'CTAG_G Cuts at: 2792 2792 Size: 5650 BamHI G'GATC_C Cuts at: 2980 3347 2980 Size: 367 5283 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2971 4426 618 Size: 325 217 627 1184 1455 1842 Fragments arranged by size: 1842 1455 1184 627 325 217 BanII G_rGCy'C Cuts at: 1391 2346 2450 2809 2969 1391 Size: 955 104 359 160 4072 Fragments arranged by size: 4072 955 359 160 104 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2101 2611 928 Size: 971 202 510 3967 Fragments arranged by size: 3967 971 510 202 BbvCI CC'TCA_GC Cuts at: 2195 2195 Size: 5650 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3841 3875 1903 Size: 34 1904 34 3678 Fragments arranged by size: 3678 1904 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3542 4752 3542 Size: 1210 4440 BclI T'GATC_A Cuts at: 1548 1548 Size: 5650 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4261 136 Size: 107 122 71 631 605 2589 1525 Fragments arranged by size: 2589 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5645 5645 Size: 5650 BlpI GC'TnA_GC Cuts at: 3067 3067 Size: 5650 Bme1580I G_kGCm'C Cuts at: 1790 2809 2908 3711 4867 1790 Size: 1019 99 803 1156 2573 Fragments arranged by size: 2573 1156 1019 803 99 BmgBI CAC'GTC Cuts at: 1318 2013 1318 Size: 695 4955 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2451 3381 4339 Size: 416 53 83 399 24 1034 930 958 Cuts at: 4339 442 Size: 1753 Fragments arranged by size: 1753 1034 958 930 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5650 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2417 4310 1239 Size: 1178 1893 2579 Fragments arranged by size: 2579 1893 1178 Bpu10I CC'TnA_GC Cuts at: 2195 2700 2195 Size: 505 5145 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2539 2902 3775 4640 4938 2539 Size: 363 873 865 298 3251 Fragments arranged by size: 3251 873 865 363 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4313 882 Size: 617 2814 2219 Fragments arranged by size: 2814 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3441 493 Size: 711 2237 2702 Fragments arranged by size: 2702 2237 711 BsaBI GATnn'nnATC Cuts at: 5644 5644 Size: 5650 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3457 3839 4474 275 Size: 53 83 186 936 1924 382 635 1451 Fragments arranged by size: 1924 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4083 4481 4755 4902 5313 4083 Size: 398 274 147 411 4420 Fragments arranged by size: 4420 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5425 Fragments arranged by size: 5425 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 2722 1186 Size: 943 593 4114 Fragments arranged by size: 4114 943 593 BseYI C'CCAG_C Cuts at: 2350 2810 4853 5349 2350 Size: 460 2043 496 2651 Fragments arranged by size: 2651 2043 496 460 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5077 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3100 3861 4889 1540 Size: 275 186 1099 761 1028 2301 Fragments arranged by size: 2301 1099 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2450 3711 3796 4867 2450 Size: 1261 85 1071 3233 Fragments arranged by size: 3233 1261 1071 85 BsmI GAATG_Cn' Cuts at: 3161 3254 3161 Size: 93 5557 BsmBI CGTCTCn'nnnn_ Cuts at: 2003 2003 Size: 5650 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2346 2450 2809 2908 2969 3711 3796 Size: 399 556 104 359 99 61 742 85 Cuts at: 3796 4867 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 556 399 359 104 99 85 61 BspEI T'CCGG_A Cuts at: 5313 5313 Size: 5650 BspHI T'CATG_A Cuts at: 2412 3539 5269 2412 Size: 1127 1730 2793 Fragments arranged by size: 2793 1730 1127 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2735 3005 844 Size: 1891 270 3489 Fragments arranged by size: 3489 1891 270 BsrBI CCG'CTC Cuts at: 1394 1823 3537 1394 Size: 429 1714 3507 Fragments arranged by size: 3507 1714 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4145 4319 65 Size: 1183 2897 174 1396 Fragments arranged by size: 2897 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4294 4481 1700 Size: 110 2484 187 2869 Fragments arranged by size: 2869 2484 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5650 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5650 BssSI C'ACGA_G Cuts at: 3710 4722 3710 Size: 1012 4638 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5650 BstBI TT'CG_AA Cuts at: 4454 4454 Size: 5650 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5650 BstXI CCAn_nnnn'nTGG Cuts at: 2441 2650 2441 Size: 209 5441 BstYI r'GATC_y Cuts at: 1972 2333 2980 3347 3732 3749 5201 5287 5299 Size: 361 647 367 385 17 1452 86 12 Cuts at: 5299 5645 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 647 385 367 361 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2786 2786 Size: 5650 BtgI C'CryG_G Cuts at: 513 1065 2004 2094 513 Size: 552 939 90 4069 Fragments arranged by size: 4069 939 552 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4911 Fragments arranged by size: 4911 683 56 BtsI GCAGTG_nn' Cuts at: 3166 3960 3980 3166 Size: 794 20 4836 Fragments arranged by size: 4836 794 20 ClaI AT'CG_AT Cuts at: 1554 2469 1554 Size: 915 4735 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5615 DraI TTT'AAA Cuts at: 2954 2954 Size: 5650 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4657 809 Size: 507 3341 1802 Fragments arranged by size: 3341 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2379 2721 3097 Size: 54 1425 50 275 18 549 342 376 Cuts at: 3097 3986 5615 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 549 376 342 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3097 1537 Size: 275 1285 4090 Fragments arranged by size: 4090 1285 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2093 2636 2762 3580 1353 Size: 740 543 126 818 3423 Fragments arranged by size: 3423 818 740 543 126 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4237 4611 4757 387 Size: 1064 138 2648 374 146 1280 Fragments arranged by size: 2648 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2448 2448 Size: 5650 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2417 3713 4310 5097 5365 1239 Size: 393 785 1296 597 787 268 1524 Fragments arranged by size: 1524 1296 787 785 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2716 2806 2716 Size: 90 5560 EcoRI G'AATT_C Cuts at: 2959 3337 2959 Size: 378 5272 FspI TGC'GCA Cuts at: 4156 4156 Size: 5650 HaeII r_GCGC'y Cuts at: 1678 4797 1678 Size: 3119 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2413 2445 2537 2569 4291 Size: 32 135 32 841 32 92 32 1722 Cuts at: 4291 4323 4365 4397 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1722 841 135 92 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2994 3240 669 Size: 988 281 1056 246 3079 Fragments arranged by size: 3079 1056 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5650 HpaI GTT'AAC Cuts at: 3240 3240 Size: 5650 KpnI G_GTAC'C Cuts at: 2975 2975 Size: 5650 MfeI C'AATT_G Cuts at: 3249 3249 Size: 5650 MluI A'CGCG_T Cuts at: 5526 5526 Size: 5650 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4764 4948 1305 Size: 3459 184 2007 Fragments arranged by size: 3459 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2381 2723 10 Size: 54 1425 343 549 342 2937 Fragments arranged by size: 2937 1425 549 343 342 54 MslI CAynn'nnrTG Cuts at: 518 1166 2156 2180 2411 2648 3608 3967 4126 Size: 648 990 24 231 237 960 359 159 Cuts at: 4126 518 Size: 2042 Fragments arranged by size: 2042 990 960 648 359 237 231 159 24 MspA1I CmG'CkG Cuts at: 1606 2747 2928 3066 3743 4891 5136 1606 Size: 1141 181 138 677 1148 245 2120 Fragments arranged by size: 2120 1148 1141 677 245 181 138 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4159 Fragments arranged by size: 4159 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5650 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5650 NotI GC'GGCC_GC Cuts at: 3097 3097 Size: 5650 NspI r_CATG'y Cuts at: 1132 1229 3008 4553 1132 Size: 97 1779 1545 2229 Fragments arranged by size: 2229 1779 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5650 PciI A'CATG_T Cuts at: 4549 4549 Size: 5650 PflMI CCAn_nnn'nTGG Cuts at: 1108 2488 1108 Size: 1380 4270 PmeI GTTT'AAAC Cuts at: 2954 2954 Size: 5650 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3719 3751 5232 5264 3719 Size: 32 1481 32 4105 Fragments arranged by size: 4105 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2747 1613 Size: 1134 4516 PsiI TTA'TAA Cuts at: 3220 3220 Size: 5650 PspOMI G'GGCC_C Cuts at: 2805 2805 Size: 5650 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2012 2044 2012 Size: 32 5618 PstI C_TGCA'G Cuts at: 830 2933 3002 830 Size: 2103 69 3478 Fragments arranged by size: 3478 2103 69 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5650 PvuII CAG'CTG Cuts at: 2747 2747 Size: 5650 SacI G_AGCT'C Cuts at: 2450 2450 Size: 5650 SalI G'TCGA_C Cuts at: 1936 2992 1936 Size: 1056 4594 SbfI CC_TGCA'GG Cuts at: 3002 3002 Size: 5650 ScaI AGT'ACT Cuts at: 2071 3898 5610 2071 Size: 1827 1712 2111 Fragments arranged by size: 2111 1827 1712 SfcI C'TryA_G Cuts at: 826 1045 2929 2998 4133 4814 5005 826 Size: 219 1884 69 1135 681 191 1471 Fragments arranged by size: 1884 1471 1135 681 219 191 69 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5650 SmaI CCC'GGG Cuts at: 1872 2977 1872 Size: 1105 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2518 2881 3754 4655 4917 820 Size: 197 926 575 363 873 901 262 1553 Fragments arranged by size: 1553 926 901 873 575 363 262 197 SnaBI TAC'GTA Cuts at: 493 3441 493 Size: 2948 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5650 SphI G_CATG'C Cuts at: 1132 3008 1132 Size: 1876 3774 SspI AAT'ATT Cuts at: 5 52 3574 5 Size: 47 3522 2081 Fragments arranged by size: 3522 2081 47 StuI AGG'CCT Cuts at: 1517 2705 2791 1517 Size: 1188 86 4376 Fragments arranged by size: 4376 1188 86 StyI C'CwwG_G Cuts at: 513 1065 2004 2792 3089 513 Size: 552 939 788 297 3074 Fragments arranged by size: 3074 939 788 552 297 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2005 2406 3677 3694 3847 4032 4477 Size: 1255 425 401 1271 17 153 185 445 Cuts at: 4477 325 Size: 1498 Fragments arranged by size: 1498 1271 1255 445 425 401 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2069 2184 3896 Size: 274 80 33 51 1443 92 115 1712 Cuts at: 3896 5608 96 Size: 1712 138 Fragments arranged by size: 1712 1712 1443 274 138 115 92 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5610 5642 5610 Size: 32 5618 Tth111I GACn'n_nGTC Cuts at: 1316 2081 1316 Size: 765 4885 XbaI T'CTAG_A Cuts at: 2986 2986 Size: 5650 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2287 2287 Size: 5650 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5650 XmaI C'CCGG_G Cuts at: 1870 2975 1870 Size: 1105 4545 XmnI GAAnn'nnTTC Cuts at: 2534 3779 2534 Size: 1245 4405 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3458 276 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI