################################## # NO CUT ENZYMES ( 192 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DpnII DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MluI MroI MseI MunI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 25 enzymes) # ################################### ApaI 293 GGGCCC GGGCC^C BalI 140 TGGCCA TGG^CCA BanI 154 GGCACC G^G[CT][GA]CC BpmI 106 CTGGAG CTGGAG(16/14) BstF5I 195 CATCC GGATG(2/0) BstXI 77 CCAAGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 6 GCTCGC GC[ACGT]^[ACGT]GC CfoI 103 GCGC GCG^C DsaI 257 CCGTGG C^C[GA][CT]GG EcoNI 210 CCTCATAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 165 CCCGC CCCGC(4/6) FokI 195 CATCC GGATG(9/13) HhaI 103 GCGC GCG^C HinP1I 103 GCGC G^CGC HinfI 109 GAGTC G^A[ACGT]TC MluNI 140 TGGCCA TGG^CCA MscI 140 TGGCCA TGG^CCA MslI 195 CATCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PflMI 252 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 109 GAGTC GAGTC(4/5) PstI 42 CTGCAG CTGCA^G SfaNI 194 GCATC GCATC(5/9) SfcI 42 CTGCAG C^T[GA][CT]AG TspRI 302 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 252 CCAGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AvaII 134 GGACC G^G[AT]CC 247 GGTCC BcnI 125 CCGGG CC^[GC]GG 131 CCGGG BlpI 180 GCTGAGC GC^T[ACGT]AGC 238 GCTGAGC Bpu1102I 180 GCTGAGC GC^T[ACGT]AGC 238 GCTGAGC BsmFI 133 GGGAC GGGAC(10/14) 248 GTCCC CelII 180 GCTGAGC GC^T[ACGT]AGC 238 GCTGAGC EaeI 74 CGGCCA [CT]^GGCC[GA] 140 TGGCCA HapII 125 CCGG C^CGG 131 CCGG HpaII 125 CCGG C^CGG 131 CCGG MspA1I 50 CCGCTG C[AC]G^C[GT]G 166 CCGCTG MspI 125 CCGG C^CGG 131 CCGG NciI 125 CCGGG CC^[GC]GG 131 CCGGG PpuMI 133 GGGACCC [GA]G^G[AT]CC[CT] 246 GGGTCCC ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AciI AluI BanII BbvI BglI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI HaeIII ItaI MnlI MvaI MwoI NlaIV Sau96I ScrFI SfiI TseI