################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## Acc65I AclI AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbeI BcgI BciVI BclI BfaI BglI BlnI BsaBI BsiEI BsiWI BsmBI BsmI BspDI BspHI BspLU11I BsrDI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV FbaI FseI FspI HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI TfiI XbaI XcmI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AatII 406 GACGTC GACGT^C AccI 384 GTCGAC GT^[AC][GT]AC AccIII 308 TCCGGA T^CCGGA AfaI 1275 GTAC GT^AC AflII 400 CTTAAG C^TTAAG AflIII 1164 ACACGT A^C[GA][CT]GT AlwI 138 GATCC GGATC(4/5) AocI 661 CCTGAGG CC^T[ACGT]AGG ApaI 824 GGGCCC GGGCC^C Asp700I 756 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 25 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 628 CACGTG CAC^GTG BfrI 400 CTTAAG C^TTAAG BlpI 201 GCTGAGC GC^T[ACGT]AGC Bpu1102I 201 GCTGAGC GC^T[ACGT]AGC BsaAI 628 CACGTG [CT]AC^GT[GA] BsaI 355 GAGACC GGTCTC(1/5) BseAI 308 TCCGGA T^CCGGA BsgI 475 CTGCAC GTGCAG(16/14) Bsp1407I 1274 TGTACA T^GTACA BspEI 308 TCCGGA T^CCGGA BspMI 1200 GCAGGT ACCTGC(4/8) BsrFI 285 GCCGGT [GA]^CCGG[CT] BsrGI 1274 TGTACA T^GTACA BstAPI 144 GCACCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 30 GGTCACC G^GT[ACGT]ACC BstPI 30 GGTCACC G^GT[ACGT]ACC Bsu36I 661 CCTGAGG CC^T[ACGT]AGG CelII 201 GCTGAGC GC^T[ACGT]AGC Cfr10I 285 GCCGGT [GA]^CCGG[CT] DraIII 145 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 661 CCTGAGG CC^T[ACGT]AGG EcoO65I 30 GGTCACC G^GT[ACGT]ACC EcoT22I 454 ATGCAT ATGCA^T HaeII 108 AGCGCC [GA]GCGC^[CT] HinfI 382 GAGTC G^A[ACGT]TC MroI 308 TCCGGA T^CCGGA MseI 401 TTAA T^TAA NsiI 454 ATGCAT ATGCA^T NspI 800 GCATGC [GA]CATG^[CT] PaeR7I 982 CTCGAG C^TCGAG PflFI 25 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 382 GAGTC GAGTC(4/5) PmaCI 628 CACGTG CAC^GTG PmlI 628 CACGTG CAC^GTG PpuMI 692 AGGACCC [GA]G^G[AT]CC[CT] RsaI 1275 GTAC GT^AC SalI 384 GTCGAC G^TCGAC SphI 800 GCATGC GCATG^C SspBI 1274 TGTACA T^GTACA StuI 677 AGGCCT AGG^CCT Tru9I 401 TTAA T^TAA Tth111I 25 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 982 CTCGAG C^TCGAG XmnI 756 GAAATGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 40 enzymes) # ################################### AccII 859 CGCG CG^CG 1036 CGCG AcsI 1008 GAATTT [GA]^AATT[CT] 1180 AAATTC AcyI 406 GACGTC G[GA]^CG[CT]C 1252 GACGCC ApoI 1008 GAATTT [GA]^AATT[CT] 1180 AAATTC BbsI 942 GAAGAC GAAGAC(2/6) 1392 GAAGAC BbvCI 45 CCTCAGC CCTCAGC(-5/-2) 116 CCTCAGC BglII 794 AGATCT A^GATCT 1375 AGATCT BpuAI 942 GAAGAC GAAGAC(2/6) 1392 GAAGAC BsaHI 406 GACGTC G[GA]^CG[CT]C 1252 GACGCC BsaWI 308 TCCGGA [AT]^CCGG[AT] 443 ACCGGA BseRI 379 GAGGAG GAGGAG(10/8) 664 GAGGAG BsmAI 355 GAGAC GTCTC(1/5) 928 GTCTC BsmFI 368 GGGAC GGGAC(10/14) 1238 GTCCC BsrBI 1050 CCGCTC CCGCTC(-3/-3) 640 GAGCGG BsrI 609 ACTGG ACTGG(1/-1) 636 ACTGG BstUI 859 CGCG CG^CG 1036 CGCG BstXI 734 CCAGCAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1196 CCAAGCAGGTGG BstYI 794 AGATCT [GA]^GATC[CT] 1375 AGATCT DraII 692 AGGACCC [GA]G^G[ACGT]CC[CT] 823 AGGGCCC EarI 214 CTCTTC CTCTTC(1/4) 447 GAAGAG EcoNI 359 CCTTCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1128 CCTGAATGAGG EcoO109I 692 AGGACCC [GA]G^G[ACGT]CC[CT] 823 AGGGCCC EcoT14I 364 CCAAGG C^C[AT][AT]GG 582 CCTTGG FauI 857 CCCGC CCCGC(4/6) 1037 GCGGG HgaI 9 GACGC GACGC(5/10) 1252 GACGC Hin1I 406 GACGTC G[GA]^CG[CT]C 1252 GACGCC HincII 384 GTCGAC GT[CT]^[GA]AC 1168 GTCAAC HindII 384 GTCGAC GT[CT]^[GA]AC 1168 GTCAAC Ksp632I 214 CTCTTC CTCTTC(1/4) 447 GAAGAG MflI 794 AGATCT [GA]^GATC[CT] 1375 AGATCT MslI 846 CATCGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 912 CATGAACATG MvnI 859 CGCG CG^CG 1036 CGCG PflMI 141 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 480 CCAGAAGCTGG PstI 331 CTGCAG CTGCA^G 591 CTGCAG SexAI 146 ACCTGGT A^CC[AT]GGT 779 ACCTGGT SmlI 400 CTTAAG C^T[CT][GA]AG 982 CTCGAG StyI 364 CCAAGG C^C[AT][AT]GG 582 CCTTGG Tsp509I 1009 AATT ^AATT 1181 AATT Van91I 141 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 480 CCAGAAGCTGG XhoII 794 AGATCT [GA]^GATC[CT] 1375 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AluI AlwNI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsoBI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PvuII SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI TaqI TseI Tsp45I TspRI TthHB8I XmaCI XmaI