(Circular) (Six-base) MAPSORT of: pFN21AE4092.seq Check: 7356 from: 1 to: 6103 pFN21AE4092 6103 bp With 182 enzymes: SgfI * March 28, 2012 13:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3196 3196 Size: 6103 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2415 3913 278 Size: 53 83 186 936 879 1498 2468 Fragments arranged by size: 2468 1498 936 879 186 83 53 AccI GT'mk_AC Cuts at: 1937 2390 3446 1937 Size: 453 1056 4594 Fragments arranged by size: 4594 1056 453 Acc65I G'GTAC_C Cuts at: 3424 3424 Size: 6103 AclI AA'CG_TT Cuts at: 1563 4230 4603 1563 Size: 2667 373 3063 Fragments arranged by size: 3063 2667 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2710 2963 3088 3280 4166 5550 5818 1632 Size: 1078 253 125 192 886 1384 268 1917 Fragments arranged by size: 1917 1384 1078 886 268 253 192 125 AflII C'TTAA_G Cuts at: 820 1017 2405 820 Size: 197 1388 4518 Fragments arranged by size: 4518 1388 197 AflIII A'CryG_T Cuts at: 1203 3169 5002 5979 1203 Size: 1966 1833 977 1327 Fragments arranged by size: 1966 1833 1327 977 AgeI A'CCGG_T Cuts at: 4934 4934 Size: 6103 AhdI GACnn_n'nnGTC Cuts at: 4832 4832 Size: 6103 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6103 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6103 AlwNI CAG_nnn'CTG Cuts at: 2017 2152 2281 2479 2491 2626 3846 5418 2017 Size: 135 129 198 12 135 1220 1572 2702 Fragments arranged by size: 2702 1572 1220 198 135 135 129 12 ApaI G_GGCC'C Cuts at: 2833 2833 Size: 6103 ApaLI G'TGCA_C Cuts at: 4160 5316 4160 Size: 1156 4947 ApoI r'AATT_y Cuts at: 1493 3013 3185 3412 3638 3790 5899 1493 Size: 1520 172 227 226 152 2109 1697 Fragments arranged by size: 2109 1697 1520 227 226 172 152 AseI AT'TA_AT Cuts at: 160 1032 4657 160 Size: 872 3625 1606 Fragments arranged by size: 3625 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 AvaI C'yCGr_G Cuts at: 1870 1943 2177 2232 2247 2307 2987 3428 1870 Size: 73 234 55 15 60 680 441 4545 Fragments arranged by size: 4545 680 441 234 73 60 55 15 BamHI G'GATC_C Cuts at: 3433 3800 3433 Size: 367 5736 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2155 2400 2528 3424 4879 Size: 325 217 627 368 245 128 896 1455 Cuts at: 4879 618 Size: 1842 Fragments arranged by size: 1842 1455 896 627 368 325 245 217 128 BanII G_rGCy'C Cuts at: 1391 2073 2213 2232 2241 2586 2833 2930 3341 Size: 682 140 19 9 345 247 97 411 Cuts at: 3341 3422 1391 Size: 81 4072 Fragments arranged by size: 4072 682 411 345 247 140 97 81 19 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2954 3404 928 Size: 971 1055 450 3627 Fragments arranged by size: 3627 1055 971 450 BbvCI CC'TCA_GC Cuts at: 2051 2122 2051 Size: 71 6032 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4294 4328 1903 Size: 34 50 34 2273 34 3678 Fragments arranged by size: 3678 2273 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3995 5205 3995 Size: 1210 4893 BclI T'GATC_A Cuts at: 1548 1548 Size: 6103 BfrBI ATG'CAT Cuts at: 2461 2461 Size: 6103 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4714 136 Size: 107 122 71 631 605 3042 1525 Fragments arranged by size: 3042 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2799 3380 6098 2799 Size: 581 2718 2804 Fragments arranged by size: 2804 2718 581 BlpI GC'TnA_GC Cuts at: 2207 3520 2207 Size: 1313 4790 Bme1580I G_kGCm'C Cuts at: 1790 2160 2403 2833 4164 5320 1790 Size: 370 243 430 1331 1156 2573 Fragments arranged by size: 2573 1331 1156 430 370 243 BmgBI CAC'GTC Cuts at: 1318 2857 3172 1318 Size: 1539 315 4249 Fragments arranged by size: 4249 1539 315 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3834 4792 442 Size: 416 53 83 399 24 2417 958 1753 Fragments arranged by size: 2417 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6103 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2663 2768 2920 3212 4763 1239 Size: 1424 105 152 292 1551 2579 Fragments arranged by size: 2579 1551 1424 292 152 105 Bpu10I CC'TnA_GC Cuts at: 2051 2122 2051 Size: 71 6032 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4228 5093 5391 4228 Size: 865 298 4940 Fragments arranged by size: 4940 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2354 4766 882 Size: 617 855 2412 2219 Fragments arranged by size: 2412 2219 855 617 BsaAI yAC'GTr Cuts at: 493 1204 2635 3894 493 Size: 711 1431 1259 2702 Fragments arranged by size: 2702 1431 1259 711 BsaBI GATnn'nnATC Cuts at: 6097 6097 Size: 6103 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2412 3258 3910 4292 Size: 53 83 186 936 879 846 652 382 Cuts at: 4292 4927 275 Size: 635 1451 Fragments arranged by size: 1451 936 879 846 652 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2313 2448 4536 4934 5208 5355 5766 2313 Size: 135 2088 398 274 147 411 2650 Fragments arranged by size: 2650 2088 411 398 274 147 135 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2876 2906 1178 Size: 30 165 30 1473 30 4375 Fragments arranged by size: 4375 1473 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2399 2684 1186 Size: 1213 285 4605 Fragments arranged by size: 4605 1213 285 BseYI C'CCAG_C Cuts at: 2090 2619 5306 5802 2090 Size: 529 2687 496 2391 Fragments arranged by size: 2687 2391 529 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2465 1163 Size: 573 729 4801 Fragments arranged by size: 4801 729 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3553 4314 5342 1540 Size: 275 186 1552 761 1028 2301 Fragments arranged by size: 2301 1552 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2213 2241 2586 2994 4164 4249 5320 2213 Size: 28 345 408 1170 85 1071 2996 Fragments arranged by size: 2996 1170 1071 408 345 85 28 BsmI GAATG_Cn' Cuts at: 3614 3707 3614 Size: 93 6010 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2160 2213 2232 2241 2403 2586 Size: 399 283 87 53 19 9 162 183 Cuts at: 2586 2833 2930 2994 3341 3422 4164 4249 5320 Size: 247 97 64 347 81 742 85 1071 Cuts at: 5320 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 347 283 247 183 162 97 87 85 81 64 53 19 9 BspEI T'CCGG_A Cuts at: 2313 5766 2313 Size: 3453 2650 BspHI T'CATG_A Cuts at: 3992 5722 3992 Size: 1730 4373 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3196 3458 844 Size: 2352 262 3489 Fragments arranged by size: 3489 2352 262 BsrBI CCG'CTC Cuts at: 1394 1823 2647 3057 3990 1394 Size: 429 824 410 933 3507 Fragments arranged by size: 3507 933 824 429 410 BsrDI GCAATG_nn' Cuts at: 65 1248 4598 4772 65 Size: 1183 3350 174 1396 Fragments arranged by size: 3350 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2290 4747 4934 1700 Size: 110 480 2457 187 2869 Fragments arranged by size: 2869 2457 480 187 110 BsrGI T'GTAC_A Cuts at: 96 3279 96 Size: 3183 2920 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6103 BssSI C'ACGA_G Cuts at: 4163 5175 4163 Size: 1012 5091 BstAPI GCAn_nnn'nTGC Cuts at: 794 2155 794 Size: 1361 4742 BstBI TT'CG_AA Cuts at: 4907 4907 Size: 6103 BstEII G'GTnAC_C Cuts at: 1184 2035 1184 Size: 851 5252 BstXI CCAn_nnnn'nTGG Cuts at: 2746 3208 2746 Size: 462 5641 BstYI r'GATC_y Cuts at: 1972 2799 3380 3433 3800 4185 4202 5654 5740 Size: 827 581 53 367 385 17 1452 86 Cuts at: 5740 5752 6098 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 827 581 385 367 346 86 53 17 12 Bsu36I CC'TnA_GG Cuts at: 2667 2667 Size: 6103 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5551 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2410 487 Size: 683 56 1184 4180 Fragments arranged by size: 4180 1184 683 56 BtsI GCAGTG_nn' Cuts at: 3619 4413 4433 3619 Size: 794 20 5289 Fragments arranged by size: 5289 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6103 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6068 DraI TTT'AAA Cuts at: 3407 3407 Size: 6103 DraIII CAC_nnn'GTG Cuts at: 2155 2155 Size: 6103 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5110 809 Size: 507 3794 1802 Fragments arranged by size: 3794 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2346 2706 2901 Size: 54 1425 50 275 18 516 360 195 Cuts at: 2901 2970 3198 3550 4439 6068 8 Size: 69 228 352 889 1629 43 Fragments arranged by size: 1629 1425 889 516 360 352 275 228 195 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3550 1537 Size: 275 1738 4090 Fragments arranged by size: 4090 1738 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2225 2447 4033 1353 Size: 872 222 1586 3423 Fragments arranged by size: 3423 1586 872 222 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4690 5064 5210 387 Size: 1064 138 3101 374 146 1280 Fragments arranged by size: 3101 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2211 2239 2584 2211 Size: 28 345 5730 Fragments arranged by size: 5730 345 28 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2663 2710 2768 2920 2963 3088 3212 Size: 393 1031 47 58 152 43 125 124 Cuts at: 3212 3280 4166 4763 5550 5818 1239 Size: 68 886 597 787 268 1524 Fragments arranged by size: 1524 1031 886 787 597 393 268 152 125 124 68 58 47 43 EcoNI CCTnn'n_nnAGG Cuts at: 2368 3137 2368 Size: 769 5334 EcoO109I rG'GnC_Cy Cuts at: 2698 2829 2698 Size: 131 5972 EcoRI G'AATT_C Cuts at: 3412 3790 3412 Size: 378 5725 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2740 2740 Size: 6103 FspI TGC'GCA Cuts at: 4609 4609 Size: 6103 HaeII r_GCGC'y Cuts at: 1678 2117 5250 1678 Size: 439 3133 2531 Fragments arranged by size: 3133 2531 439 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2446 2478 4744 4776 4818 Size: 32 135 32 874 32 2266 32 42 Cuts at: 4818 4850 1373 Size: 32 2626 Fragments arranged by size: 2626 2266 874 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2391 3175 3447 3693 669 Size: 988 281 453 784 272 246 3079 Fragments arranged by size: 3079 988 784 453 281 272 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6103 HpaI GTT'AAC Cuts at: 3693 3693 Size: 6103 KpnI G_GTAC'C Cuts at: 3428 3428 Size: 6103 MfeI C'AATT_G Cuts at: 3702 3702 Size: 6103 MluI A'CGCG_T Cuts at: 5979 5979 Size: 6103 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2052 5217 5401 1305 Size: 747 3165 184 2007 Fragments arranged by size: 3165 2007 747 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2708 2903 3200 10 Size: 54 1425 343 876 195 297 2913 Fragments arranged by size: 2913 1425 876 343 297 195 54 MslI CAynn'nnrTG Cuts at: 518 1166 2855 2921 4061 4420 4579 518 Size: 648 1689 66 1140 359 159 2042 Fragments arranged by size: 2042 1689 1140 648 359 159 66 MspA1I CmG'CkG Cuts at: 1606 2281 2341 2626 3519 4196 5344 5589 1606 Size: 675 60 285 893 677 1148 245 2120 Fragments arranged by size: 2120 1148 893 677 675 285 245 60 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5551 NdeI CA'TA_TG Cuts at: 387 387 Size: 6103 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6103 NotI GC'GGCC_GC Cuts at: 3550 3550 Size: 6103 NsiI A_TGCA'T Cuts at: 2463 2463 Size: 6103 NspI r_CATG'y Cuts at: 1132 1229 2809 3461 5006 1132 Size: 97 1580 652 1545 2229 Fragments arranged by size: 2229 1580 1545 652 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6103 PciI A'CATG_T Cuts at: 5002 5002 Size: 6103 PflMI CCAn_nnn'nTGG Cuts at: 1108 2152 2491 1108 Size: 1044 339 4720 Fragments arranged by size: 4720 1044 339 PfoI T'CCnGG_A Cuts at: 2103 2379 3189 2103 Size: 276 810 5017 Fragments arranged by size: 5017 810 276 PmeI GTTT'AAAC Cuts at: 3407 3407 Size: 6103 PmlI CAC'GTG Cuts at: 2635 2635 Size: 6103 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4172 4204 5685 5717 4172 Size: 32 1481 32 4558 Fragments arranged by size: 4558 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2698 2698 Size: 6103 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6103 PsiI TTA'TAA Cuts at: 3673 3673 Size: 6103 PspOMI G'GGCC_C Cuts at: 2829 2829 Size: 6103 PspXI vC'TCGA_Gb Cuts at: 2987 2987 Size: 6103 PstI C_TGCA'G Cuts at: 830 2340 2600 3455 830 Size: 1510 260 855 3478 Fragments arranged by size: 3478 1510 855 260 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6103 PvuII CAG'CTG Cuts at: 2281 2341 2626 2281 Size: 60 285 5758 Fragments arranged by size: 5758 285 60 SacI G_AGCT'C Cuts at: 2213 2241 2586 2213 Size: 28 345 5730 Fragments arranged by size: 5730 345 28 SalI G'TCGA_C Cuts at: 1936 2389 3445 1936 Size: 453 1056 4594 Fragments arranged by size: 4594 1056 453 SbfI CC_TGCA'GG Cuts at: 3455 3455 Size: 6103 ScaI AGT'ACT Cuts at: 4351 6063 4351 Size: 1712 4391 SexAI A'CCwGG_T Cuts at: 2151 2784 2151 Size: 633 5470 SfcI C'TryA_G Cuts at: 826 1045 2336 2471 2548 2596 3025 3451 4586 Size: 219 1291 135 77 48 429 426 1135 Cuts at: 4586 5267 5458 826 Size: 681 191 1471 Fragments arranged by size: 1471 1291 1135 681 429 426 219 191 135 77 48 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6103 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6103 SmaI CCC'GGG Cuts at: 1872 2234 2249 2309 3430 1872 Size: 362 15 60 1121 4545 Fragments arranged by size: 4545 1121 362 60 15 SmlI C'TyrA_G Cuts at: 820 1017 1943 2405 2987 4207 5108 5370 820 Size: 197 926 462 582 1220 901 262 1553 Fragments arranged by size: 1553 1220 926 901 582 462 262 197 SnaBI TAC'GTA Cuts at: 493 3894 493 Size: 3401 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6103 SphI G_CATG'C Cuts at: 1132 2809 3461 1132 Size: 1677 652 3774 Fragments arranged by size: 3774 1677 652 SspI AAT'ATT Cuts at: 5 52 4027 5 Size: 47 3975 2081 Fragments arranged by size: 3975 2081 47 StuI AGG'CCT Cuts at: 1517 2684 1517 Size: 1167 4936 StyI C'CwwG_G Cuts at: 513 1065 2369 2587 3542 513 Size: 552 1304 218 955 3074 Fragments arranged by size: 3074 1304 955 552 218 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2372 2516 3284 4130 4147 4300 4485 Size: 1255 792 144 768 846 17 153 185 Cuts at: 4485 4930 325 Size: 445 1498 Fragments arranged by size: 1498 1255 846 792 768 445 185 153 144 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3279 4349 6061 Size: 274 80 33 51 1443 1302 1070 1712 Cuts at: 6061 96 Size: 138 Fragments arranged by size: 1712 1443 1302 1070 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6063 6095 6063 Size: 32 6071 Tth111I GACn'n_nGTC Cuts at: 1316 2033 1316 Size: 717 5386 XbaI T'CTAG_A Cuts at: 3439 3439 Size: 6103 XhoI C'TCGA_G Cuts at: 1943 2987 1943 Size: 1044 5059 XmaI C'CCGG_G Cuts at: 1870 2232 2247 2307 3428 1870 Size: 362 15 60 1121 4545 Fragments arranged by size: 4545 1121 362 60 15 XmnI GAAnn'nnTTC Cuts at: 2765 4232 2765 Size: 1467 4636 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2413 3911 276 Size: 53 83 186 936 879 1498 2468 Fragments arranged by size: 2468 1498 936 879 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI