(Linear) (Six-base) MAPSORT of: KIEE4092.seq Check: 1428 from: 1 to: 1398 KIEE4092.orf With 182 enzymes: SgfI * March 22, 2012 17:21 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1191 1398 Size: 1191 207 AatII G_ACGT'C Cuts at: 0 410 1398 Size: 410 988 AccI GT'mk_AC Cuts at: 0 385 1398 Size: 385 1013 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 705 958 1083 1275 1398 Size: 705 253 125 192 123 Fragments arranged by size: 705 253 192 125 123 AflII C'TTAA_G Cuts at: 0 400 1398 Size: 400 998 AflIII A'CryG_T Cuts at: 0 1164 1398 Size: 1164 234 AlwNI CAG_nnn'CTG Cuts at: 0 12 147 276 474 486 621 1398 Size: 12 135 129 198 12 135 777 Fragments arranged by size: 777 198 135 135 129 12 12 ApaI G_GGCC'C Cuts at: 0 828 1398 Size: 828 570 ApoI r'AATT_y Cuts at: 0 1008 1180 1398 Size: 1008 172 218 Fragments arranged by size: 1008 218 172 AvaI C'yCGr_G Cuts at: 0 172 227 242 302 982 1398 Size: 172 55 15 60 680 416 Fragments arranged by size: 680 416 172 60 55 15 BanI G'GyrC_C Cuts at: 0 150 395 523 1398 Size: 150 245 128 875 Fragments arranged by size: 875 245 150 128 BanII G_rGCy'C Cuts at: 0 68 208 227 236 581 828 925 1336 Size: 68 140 19 9 345 247 97 411 Cuts at: 1336 1398 Size: 62 Fragments arranged by size: 411 345 247 140 97 68 62 19 9 BbsI GAAGACnn'nnnn_ Cuts at: 0 949 1398 Size: 949 449 BbvCI CC'TCA_GC Cuts at: 0 46 117 1398 Size: 46 71 1281 Fragments arranged by size: 1281 71 46 BfrBI ATG'CAT Cuts at: 0 456 1398 Size: 456 942 BglII A'GATC_T Cuts at: 0 794 1375 1398 Size: 794 581 23 Fragments arranged by size: 794 581 23 BlpI GC'TnA_GC Cuts at: 0 202 1398 Size: 202 1196 Bme1580I G_kGCm'C Cuts at: 0 155 398 828 1398 Size: 155 243 430 570 Fragments arranged by size: 570 430 243 155 BmgBI CAC'GTC Cuts at: 0 852 1167 1398 Size: 852 315 231 Fragments arranged by size: 852 315 231 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 658 763 915 1207 1398 Size: 658 105 152 292 191 Fragments arranged by size: 658 292 191 152 105 Bpu10I CC'TnA_GC Cuts at: 0 46 117 1398 Size: 46 71 1281 Fragments arranged by size: 1281 71 46 BsaI GGTCTCn'nnnn_ Cuts at: 0 349 1398 Size: 349 1049 BsaAI yAC'GTr Cuts at: 0 630 1398 Size: 630 768 BsaHI Gr'CG_yC Cuts at: 0 407 1253 1398 Size: 407 846 145 Fragments arranged by size: 846 407 145 BsaWI w'CCGG_w Cuts at: 0 308 443 1398 Size: 308 135 955 Fragments arranged by size: 955 308 135 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 871 901 1398 Size: 871 30 497 Fragments arranged by size: 871 497 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 394 679 1398 Size: 394 285 719 Fragments arranged by size: 719 394 285 BseYI C'CCAG_C Cuts at: 0 85 614 1398 Size: 85 529 784 Fragments arranged by size: 784 529 85 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 460 1398 Size: 460 938 BsiHKAI G_wGCw'C Cuts at: 0 208 236 581 989 1398 Size: 208 28 345 408 409 Fragments arranged by size: 409 408 345 208 28 Bsp1286I G_dGCh'C Cuts at: 0 68 155 208 227 236 398 581 828 Size: 68 87 53 19 9 162 183 247 Cuts at: 828 925 989 1336 1398 Size: 97 64 347 62 Fragments arranged by size: 347 247 183 162 97 87 68 64 62 53 19 9 BspEI T'CCGG_A Cuts at: 0 308 1398 Size: 308 1090 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1191 1398 Size: 1191 207 BsrBI CCG'CTC Cuts at: 0 642 1052 1398 Size: 642 410 346 Fragments arranged by size: 642 410 346 BsrFI r'CCGG_y Cuts at: 0 285 1398 Size: 285 1113 BsrGI T'GTAC_A Cuts at: 0 1274 1398 Size: 1274 124 BstAPI GCAn_nnn'nTGC Cuts at: 0 150 1398 Size: 150 1248 BstEII G'GTnAC_C Cuts at: 0 30 1398 Size: 30 1368 BstXI CCAn_nnnn'nTGG Cuts at: 0 741 1203 1398 Size: 741 462 195 Fragments arranged by size: 741 462 195 BstYI r'GATC_y Cuts at: 0 794 1375 1398 Size: 794 581 23 Fragments arranged by size: 794 581 23 Bsu36I CC'TnA_GG Cuts at: 0 662 1398 Size: 662 736 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 405 1398 Size: 405 993 DraIII CAC_nnn'GTG Cuts at: 0 150 1398 Size: 150 1248 EaeI y'GGCC_r Cuts at: 0 341 701 896 965 1193 1398 Size: 341 360 195 69 228 205 Fragments arranged by size: 360 341 228 205 195 69 EarI CTCTTCn'nnn_ Cuts at: 0 220 442 1398 Size: 220 222 956 Fragments arranged by size: 956 222 220 EcoICRI GAG'CTC Cuts at: 0 206 234 579 1398 Size: 206 28 345 819 Fragments arranged by size: 819 345 206 28 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 658 705 763 915 958 1083 1207 1275 Size: 658 47 58 152 43 125 124 68 Cuts at: 1275 1398 Size: 123 Fragments arranged by size: 658 152 125 124 123 68 58 47 43 EcoNI CCTnn'n_nnAGG Cuts at: 0 363 1132 1398 Size: 363 769 266 Fragments arranged by size: 769 363 266 EcoO109I rG'GnC_Cy Cuts at: 0 693 824 1398 Size: 693 131 574 Fragments arranged by size: 693 574 131 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 735 1398 Size: 735 663 HaeII r_GCGC'y Cuts at: 0 112 1398 Size: 112 1286 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 441 473 1398 Size: 441 32 925 Fragments arranged by size: 925 441 32 HincII GTy'rAC Cuts at: 0 386 1170 1398 Size: 386 784 228 Fragments arranged by size: 784 386 228 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 47 1398 Size: 47 1351 MscI TGG'CCA Cuts at: 0 703 898 1195 1398 Size: 703 195 297 203 Fragments arranged by size: 703 297 203 195 MslI CAynn'nnrTG Cuts at: 0 850 916 1398 Size: 850 66 482 Fragments arranged by size: 850 482 66 MspA1I CmG'CkG Cuts at: 0 276 336 621 1398 Size: 276 60 285 777 Fragments arranged by size: 777 285 276 60 NsiI A_TGCA'T Cuts at: 0 458 1398 Size: 458 940 NspI r_CATG'y Cuts at: 0 804 1398 Size: 804 594 PflMI CCAn_nnn'nTGG Cuts at: 0 147 486 1398 Size: 147 339 912 Fragments arranged by size: 912 339 147 PfoI T'CCnGG_A Cuts at: 0 98 374 1184 1398 Size: 98 276 810 214 Fragments arranged by size: 810 276 214 98 PmlI CAC'GTG Cuts at: 0 630 1398 Size: 630 768 PpuMI rG'GwC_Cy Cuts at: 0 693 1398 Size: 693 705 PspOMI G'GGCC_C Cuts at: 0 824 1398 Size: 824 574 PspXI vC'TCGA_Gb Cuts at: 0 982 1398 Size: 982 416 PstI C_TGCA'G Cuts at: 0 335 595 1398 Size: 335 260 803 Fragments arranged by size: 803 335 260 PvuII CAG'CTG Cuts at: 0 276 336 621 1398 Size: 276 60 285 777 Fragments arranged by size: 777 285 276 60 SacI G_AGCT'C Cuts at: 0 208 236 581 1398 Size: 208 28 345 817 Fragments arranged by size: 817 345 208 28 SalI G'TCGA_C Cuts at: 0 384 1398 Size: 384 1014 SexAI A'CCwGG_T Cuts at: 0 146 779 1398 Size: 146 633 619 Fragments arranged by size: 633 619 146 SfcI C'TryA_G Cuts at: 0 331 466 543 591 1020 1398 Size: 331 135 77 48 429 378 Fragments arranged by size: 429 378 331 135 77 48 SmaI CCC'GGG Cuts at: 0 229 244 304 1398 Size: 229 15 60 1094 Fragments arranged by size: 1094 229 60 15 SmlI C'TyrA_G Cuts at: 0 400 982 1398 Size: 400 582 416 Fragments arranged by size: 582 416 400 SphI G_CATG'C Cuts at: 0 804 1398 Size: 804 594 StuI AGG'CCT Cuts at: 0 679 1398 Size: 679 719 StyI C'CwwG_G Cuts at: 0 364 582 1398 Size: 364 218 816 Fragments arranged by size: 816 364 218 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 367 511 1279 1398 Size: 367 144 768 119 Fragments arranged by size: 768 367 144 119 TatI w'GTAC_w Cuts at: 0 1274 1398 Size: 1274 124 Tth111I GACn'n_nGTC Cuts at: 0 28 1398 Size: 28 1370 XhoI C'TCGA_G Cuts at: 0 982 1398 Size: 982 416 XmaI C'CCGG_G Cuts at: 0 227 242 302 1398 Size: 227 15 60 1096 Fragments arranged by size: 1096 227 60 15 XmnI GAAnn'nnTTC Cuts at: 0 760 1398 Size: 760 638 ZraI GAC'GTC Cuts at: 0 408 1398 Size: 408 990 Enzymes that do cut: AarI AatII AccI AcuI AflII AflIII AlwNI ApaI ApoI AvaI BanI BanII BbsI BbvCI BfrBI BglII BlpI Bme1580I BmgBI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgZI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII MmeI MscI MslI MspA1I NsiI NspI PflMI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII SacI SalI SexAI SfcI SmaI SmlI SphI StuI StyI TaqII TatI Tth111I XhoI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI BglI BmrI BmtI BplI BpuEI BsaBI BsiEI BsiWI BsmI BsmBI BspHI BsrDI BssHII BssSI BstBI BstZ17I BtgI BtsI ClaI CspCI DraI DrdI EagI EciI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PpiI PshAI PsiI PsrI PvuI RsrII SacII SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TstI XbaI XcmI