Restriction map for
pFN21AE5168
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# NO CUT ENZYMES ( 180 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI
BstEII BstF5I BstPI BstXI Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I FbaI FokI FseI
FspI HgaI Hin1I HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MaeII MaeIII MamI MboII MfeI
MluI MluNI MroI MscI MseI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tru9I
Tsp45I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 268 GTATAC GT^[AC][GT]AC
AfaI 402 GTAC GT^AC
ApaI 28 GGGCCC GGGCC^C
AvaI 31 CCCGAG C^[CT]CG[GA]G
BanII 28 GGGCCC G[GA]GC[CT]^C
BcnI 56 CCGGG CC^[GC]GG
BmyI 28 GGGCCC G[AGT]GC[ACT]^C
BpmI 427 CTGGAG CTGGAG(16/14)
BsiYI 426 CCTGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 426 CCTGGAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 248 GAGAC GTCTC(1/5)
BsmFI 441 GTCCC GGGAC(10/14)
BsoBI 31 CCCGAG C^[CT]CG[GA]G
Bsp1286I 28 GGGCCC G[AGT]GC[ACT]^C
BsrI 283 CCAGT ACTGG(1/-1)
Bst1107I 268 GTATAC GTA^TAC
BstAPI 438 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 18 AGATCC [GA]^GATC[CT]
BstZ17I 268 GTATAC GTA^TAC
Cac8I 352 GCAGGC GC[ACGT]^[ACGT]GC
EcoNI 426 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 147 ATGCAT ATGCA^T
FauI 185 GCGGG CCCGC(4/6)
HaeII 362 AGCGCC [GA]GCGC^[CT]
HaeIII 29 GGCC GG^CC
HapII 56 CCGG C^CGG
HinfI 177 GATTC G^A[ACGT]TC
HpaII 56 CCGG C^CGG
MflI 18 AGATCC [GA]^GATC[CT]
MslI 369 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 56 CCGG C^CGG
NciI 56 CCGGG CC^[GC]GG
NsiI 147 ATGCAT ATGCA^T
RsaI 402 GTAC GT^AC
SfaNI 149 GCATC GCATC(5/9)
TfiI 177 GATTC G^A[AT]TC
TspRI 254 CACTG CA[GC]TG[ACGT][ACGT]^
XhoII 18 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 205 AAATTT [GA]^AATT[CT]
303 AAATTT
AluI 77 AGCT AG^CT
137 AGCT
AlwI 19 GATCC GGATC(4/5)
230 GGATC
ApoI 205 AAATTT [GA]^AATT[CT]
303 AAATTT
AvaII 297 GGACC G^G[AT]CC
336 GGACC
BseRI 397 GAGGAG GAGGAG(10/8)
430 GAGGAG
BsgI 344 GTGCAG GTGCAG(16/14)
436 GTGCAG
DdeI 38 CTCAG C^T[ACGT]AG
329 CTAAG
DpnII 19 GATC ^GATC
231 GATC
HincII 85 GTCAAC GT[CT]^[GA]AC
211 GTCAAC
HindII 85 GTCAAC GT[CT]^[GA]AC
211 GTCAAC
HphI 162 GGTGA GGTGA(8/7)
189 GGTGA
MboI 19 GATC ^GATC
231 GATC
NdeII 19 GATC ^GATC
231 GATC
NlaIV 28 GGGCCC GG[ACGT]^[ACGT]CC
355 GGCTCC
Sau3AI 19 GATC ^GATC
231 GATC
Tsp509I 206 AATT ^AATT
304 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AccII AciI AlwNI BbvI BsaJI
BssKI BstNI BstUI CfoI Cfr13I
EcoRII Fnu4HI HhaI HinP1I ItaI
MnlI MvaI MvnI MwoI NlaIII
Sau96I ScrFI TseI