(Circular) (Six-base) MAPSORT of: pFN21AE5168.seq Check: 8836 from: 1 to: 5164 pFN21AE5168 5164 bp With 182 enzymes: SgfI * January 30, 2014 17:14 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2974 278 Size: 53 83 186 936 1438 2468 Fragments arranged by size: 2468 1438 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2274 2507 1937 Size: 337 233 4594 Fragments arranged by size: 4594 337 233 Acc65I G'GTAC_C Cuts at: 2485 2485 Size: 5164 AclI AA'CG_TT Cuts at: 1563 3291 3664 1563 Size: 1728 373 3063 Fragments arranged by size: 3063 1728 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3227 4611 4879 1632 Size: 1595 1384 268 1917 Fragments arranged by size: 1917 1595 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4967 AflIII A'CryG_T Cuts at: 1203 4063 5040 1203 Size: 2860 977 1327 Fragments arranged by size: 2860 1327 977 AgeI A'CCGG_T Cuts at: 3995 3995 Size: 5164 AhdI GACnn_n'nnGTC Cuts at: 3893 3893 Size: 5164 AleI CACnn'nnGTG Cuts at: 1166 2378 1166 Size: 1212 3952 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5164 AlwNI CAG_nnn'CTG Cuts at: 2083 2116 2449 2907 4479 2083 Size: 33 333 458 1572 2768 Fragments arranged by size: 2768 1572 458 333 33 ApaI G_GGCC'C Cuts at: 2037 2037 Size: 5164 ApaLI G'TGCA_C Cuts at: 3221 4377 3221 Size: 1156 4008 ApoI r'AATT_y Cuts at: 1493 2210 2308 2473 2699 2851 4960 1493 Size: 717 98 165 226 152 2109 1697 Fragments arranged by size: 2109 1697 717 226 165 152 98 AseI AT'TA_AT Cuts at: 160 1032 3718 160 Size: 872 2686 1606 Fragments arranged by size: 2686 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5164 AvaI C'yCGr_G Cuts at: 1870 1943 2036 2489 1870 Size: 73 93 453 4545 Fragments arranged by size: 4545 453 93 73 BamHI G'GATC_C Cuts at: 2494 2861 2494 Size: 367 4797 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2485 3940 618 Size: 325 217 627 698 1455 1842 Fragments arranged by size: 1842 1455 698 627 325 217 BanII G_rGCy'C Cuts at: 1391 2037 2483 1391 Size: 646 446 4072 Fragments arranged by size: 4072 646 446 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4193 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3355 3389 1903 Size: 34 1418 34 3678 Fragments arranged by size: 3678 1418 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3056 4266 3056 Size: 1210 3954 BclI T'GATC_A Cuts at: 1548 1548 Size: 5164 BfrBI ATG'CAT Cuts at: 2154 2154 Size: 5164 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3775 136 Size: 107 122 71 631 605 2103 1525 Fragments arranged by size: 2103 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5159 5159 Size: 5164 BlpI GC'TnA_GC Cuts at: 2581 2581 Size: 5164 Bme1580I G_kGCm'C Cuts at: 1790 2037 3225 4381 1790 Size: 247 1188 1156 2573 Fragments arranged by size: 2573 1188 1156 247 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5164 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2282 2895 3853 Size: 416 53 83 399 24 865 613 958 Cuts at: 3853 442 Size: 1753 Fragments arranged by size: 1753 958 865 613 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5164 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2453 3824 1239 Size: 1214 1371 2579 Fragments arranged by size: 2579 1371 1214 Bpu10I CC'TnA_GC Cuts at: 2043 2043 Size: 5164 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3289 4154 4452 3289 Size: 865 298 4001 Fragments arranged by size: 4001 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3827 882 Size: 617 2328 2219 Fragments arranged by size: 2328 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2955 493 Size: 711 1751 2702 Fragments arranged by size: 2702 1751 711 BsaBI GATnn'nnATC Cuts at: 5158 5158 Size: 5164 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2971 3353 3988 275 Size: 53 83 186 936 1438 382 635 1451 Fragments arranged by size: 1451 1438 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3597 3995 4269 4416 4827 3597 Size: 398 274 147 411 3934 Fragments arranged by size: 3934 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2429 2459 1178 Size: 30 165 30 1026 30 3883 Fragments arranged by size: 3883 1026 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2417 2450 1186 Size: 1231 33 3900 Fragments arranged by size: 3900 1231 33 BseYI C'CCAG_C Cuts at: 4367 4863 4367 Size: 496 4668 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2370 2462 1163 Size: 573 634 92 3865 Fragments arranged by size: 3865 634 573 92 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2614 3375 4403 1540 Size: 275 186 613 761 1028 2301 Fragments arranged by size: 2301 1028 761 613 275 186 BsiHKAI G_wGCw'C Cuts at: 3225 3310 4381 3225 Size: 85 1071 4008 Fragments arranged by size: 4008 1071 85 BsmI GAATG_Cn' Cuts at: 2675 2768 2675 Size: 93 5071 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2037 2483 3225 3310 4381 1391 Size: 399 247 446 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 446 399 247 85 BspEI T'CCGG_A Cuts at: 4827 4827 Size: 5164 BspHI T'CATG_A Cuts at: 3053 4783 3053 Size: 1730 3434 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2519 844 Size: 1675 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3051 1394 Size: 429 1228 3507 Fragments arranged by size: 3507 1228 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3659 3833 65 Size: 1183 2411 174 1396 Fragments arranged by size: 2411 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3808 3995 1700 Size: 110 1998 187 2869 Fragments arranged by size: 2869 1998 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5164 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5164 BssSI C'ACGA_G Cuts at: 3224 4236 3224 Size: 1012 4152 BstAPI GCAn_nnn'nTGC Cuts at: 794 2449 794 Size: 1655 3509 BstBI TT'CG_AA Cuts at: 3968 3968 Size: 5164 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5164 BstYI r'GATC_y Cuts at: 1972 2023 2494 2861 3246 3263 4715 4801 4813 Size: 51 471 367 385 17 1452 86 12 Cuts at: 4813 5159 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 471 385 367 346 86 51 17 12 BstZ17I GTA'TAC Cuts at: 2275 2275 Size: 5164 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4612 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2089 487 Size: 683 56 863 3562 Fragments arranged by size: 3562 863 683 56 BtsI GCAGTG_nn' Cuts at: 2258 2680 3474 3494 2258 Size: 422 794 20 3928 Fragments arranged by size: 3928 794 422 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5164 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5129 DraI TTT'AAA Cuts at: 2468 2468 Size: 5164 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4171 809 Size: 507 2855 1802 Fragments arranged by size: 2855 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2611 3500 5129 Size: 54 1425 50 275 18 781 889 1629 Cuts at: 5129 8 Size: 43 Fragments arranged by size: 1629 1425 889 781 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2611 1537 Size: 275 799 4090 Fragments arranged by size: 4090 799 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3094 1353 Size: 1741 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2063 3751 4125 4271 387 Size: 1064 138 474 1688 374 146 1280 Fragments arranged by size: 1688 1280 1064 474 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2453 3227 3824 4611 4879 1239 Size: 393 821 774 597 787 268 1524 Fragments arranged by size: 1524 821 787 774 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2435 2435 Size: 5164 EcoRI G'AATT_C Cuts at: 2473 2851 2473 Size: 378 4786 FspI TGC'GCA Cuts at: 3670 3670 Size: 5164 HaeII r_GCGC'y Cuts at: 1678 2371 4311 1678 Size: 693 1940 2531 Fragments arranged by size: 2531 1940 693 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2429 2461 3805 3837 3879 Size: 32 135 32 857 32 1344 32 42 Cuts at: 3879 3911 1373 Size: 32 2626 Fragments arranged by size: 2626 1344 857 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2092 2218 2508 2754 669 Size: 988 281 154 126 290 246 3079 Fragments arranged by size: 3079 988 290 281 246 154 126 HindIII A'AGCT_T Cuts at: 748 748 Size: 5164 HpaI GTT'AAC Cuts at: 2754 2754 Size: 5164 KpnI G_GTAC'C Cuts at: 2489 2489 Size: 5164 MfeI C'AATT_G Cuts at: 2763 2763 Size: 5164 MluI A'CGCG_T Cuts at: 5040 5040 Size: 5164 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4278 4462 1305 Size: 2973 184 2007 Fragments arranged by size: 2973 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3342 Fragments arranged by size: 3342 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2378 3122 3481 3640 518 Size: 648 1212 744 359 159 2042 Fragments arranged by size: 2042 1212 744 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2580 3257 4405 4650 1606 Size: 974 677 1148 245 2120 Fragments arranged by size: 2120 1148 974 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4612 NdeI CA'TA_TG Cuts at: 387 387 Size: 5164 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5164 NotI GC'GGCC_GC Cuts at: 2611 2611 Size: 5164 NsiI A_TGCA'T Cuts at: 2156 2156 Size: 5164 NspI r_CATG'y Cuts at: 1132 1229 2522 4067 1132 Size: 97 1293 1545 2229 Fragments arranged by size: 2229 1545 1293 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5164 PciI A'CATG_T Cuts at: 4063 4063 Size: 5164 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5164 PmeI GTTT'AAAC Cuts at: 2468 2468 Size: 5164 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3233 3265 4746 4778 3233 Size: 32 1481 32 3619 Fragments arranged by size: 3619 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5164 PsiI TTA'TAA Cuts at: 2734 2734 Size: 5164 PspOMI G'GGCC_C Cuts at: 2033 2033 Size: 5164 PstI C_TGCA'G Cuts at: 830 2516 830 Size: 1686 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5164 SalI G'TCGA_C Cuts at: 1936 2506 1936 Size: 570 4594 SbfI CC_TGCA'GG Cuts at: 2516 2516 Size: 5164 ScaI AGT'ACT Cuts at: 3412 5124 3412 Size: 1712 3452 SfcI C'TryA_G Cuts at: 826 1045 2512 3647 4328 4519 826 Size: 219 1467 1135 681 191 1471 Fragments arranged by size: 1471 1467 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5164 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5164 SmaI CCC'GGG Cuts at: 1872 2491 1872 Size: 619 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3268 4169 4431 820 Size: 197 926 1325 901 262 1553 Fragments arranged by size: 1553 1325 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2955 493 Size: 2462 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5164 SphI G_CATG'C Cuts at: 1132 2522 1132 Size: 1390 3774 SspI AAT'ATT Cuts at: 5 52 3088 5 Size: 47 3036 2081 Fragments arranged by size: 3036 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5164 StyI C'CwwG_G Cuts at: 513 1065 2603 513 Size: 552 1538 3074 Fragments arranged by size: 3074 1538 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2319 2336 3191 3208 3361 3546 3991 Size: 1255 739 17 855 17 153 185 445 Cuts at: 3991 325 Size: 1498 Fragments arranged by size: 1498 1255 855 739 445 185 153 17 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3410 5122 96 Size: 274 80 33 51 1443 1433 1712 138 Fragments arranged by size: 1712 1443 1433 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5124 5156 5124 Size: 32 5132 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5164 XbaI T'CTAG_A Cuts at: 2500 2500 Size: 5164 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5164 XmaI C'CCGG_G Cuts at: 1870 2489 1870 Size: 619 4545 XmnI GAAnn'nnTTC Cuts at: 3293 3293 Size: 5164 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2972 276 Size: 53 83 186 936 1438 2468 Fragments arranged by size: 2468 1438 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI