(Circular) (Six-base) MAPSORT of: pFN21AE2416.seq Check: 9386 from: 1 to: 6622 pFN21AE2416 6622 bp With 182 enzymes: SgfI * July 23, 2012 18:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2195 3104 3717 2195 Size: 909 613 5100 Fragments arranged by size: 5100 909 613 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4432 278 Size: 53 83 186 936 2896 2468 Fragments arranged by size: 2896 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3965 1937 Size: 2028 4594 Acc65I G'GTAC_C Cuts at: 3468 3943 3468 Size: 475 6147 AclI AA'CG_TT Cuts at: 1563 4749 5122 1563 Size: 3186 373 3063 Fragments arranged by size: 3186 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3782 4685 6069 6337 1632 Size: 2150 903 1384 268 1917 Fragments arranged by size: 2150 1917 1384 903 268 AfeI AGC'GCT Cuts at: 3412 3412 Size: 6622 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6425 AflIII A'CryG_T Cuts at: 1203 5521 6498 1203 Size: 4318 977 1327 Fragments arranged by size: 4318 1327 977 AgeI A'CCGG_T Cuts at: 5453 5453 Size: 6622 AhdI GACnn_n'nnGTC Cuts at: 5351 5351 Size: 6622 AleI CACnn'nnGTG Cuts at: 1166 2606 1166 Size: 1440 5182 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3506 1184 Size: 2322 4300 AlwNI CAG_nnn'CTG Cuts at: 4365 5937 4365 Size: 1572 5050 ApaI G_GGCC'C Cuts at: 2165 3655 2165 Size: 1490 5132 ApaLI G'TGCA_C Cuts at: 3091 4679 5835 3091 Size: 1588 1156 3878 Fragments arranged by size: 3878 1588 1156 ApoI r'AATT_y Cuts at: 1493 3931 4157 4309 6418 1493 Size: 2438 226 152 2109 1697 Fragments arranged by size: 2438 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5176 160 Size: 872 4144 1606 Fragments arranged by size: 4144 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6622 AvaI C'yCGr_G Cuts at: 1870 1943 2128 3451 3701 3947 1870 Size: 73 185 1323 250 246 4545 Fragments arranged by size: 4545 1323 250 246 185 73 BamHI G'GATC_C Cuts at: 2124 2463 3074 3952 4319 2124 Size: 339 611 878 367 4427 Fragments arranged by size: 4427 878 611 367 339 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2297 2314 2585 2919 3230 Size: 325 217 627 510 17 271 334 311 Cuts at: 3230 3353 3468 3475 3705 3943 5398 618 Size: 123 115 7 230 238 1455 1842 Fragments arranged by size: 1842 1455 627 510 334 325 311 271 238 230 217 123 115 17 7 BanII G_rGCy'C Cuts at: 1391 2165 2492 2552 2576 2823 3060 3655 3819 Size: 774 327 60 24 247 237 595 164 Cuts at: 3819 3941 1391 Size: 122 4072 Fragments arranged by size: 4072 774 595 327 247 237 164 122 60 24 BbeI G_GCGC'C Cuts at: 2301 3234 2301 Size: 933 5689 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3838 928 Size: 971 1939 3712 Fragments arranged by size: 3712 1939 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3226 3260 3628 3662 4813 4847 1903 Size: 34 1289 34 368 34 1151 34 3678 Fragments arranged by size: 3678 1289 1151 368 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3467 4514 5724 3467 Size: 1047 1210 4365 Fragments arranged by size: 4365 1210 1047 BclI T'GATC_A Cuts at: 1548 3306 1548 Size: 1758 4864 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2237 2295 3374 Size: 107 122 71 631 605 565 58 1079 Cuts at: 3374 3659 5233 136 Size: 285 1574 1525 Fragments arranged by size: 1574 1525 1079 631 605 565 285 122 107 71 58 BglII A'GATC_T Cuts at: 2913 6617 2913 Size: 3704 2918 BlpI GC'TnA_GC Cuts at: 2178 2366 4039 2178 Size: 188 1673 4761 Fragments arranged by size: 4761 1673 188 Bme1580I G_kGCm'C Cuts at: 1790 2103 2165 3095 3480 3641 3655 3708 4683 Size: 313 62 930 385 161 14 53 975 Cuts at: 4683 5839 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 975 930 385 313 161 62 53 14 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6622 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2048 3347 4353 Size: 416 53 83 399 24 631 1299 1006 Cuts at: 4353 5311 442 Size: 958 1753 Fragments arranged by size: 1753 1299 1006 958 631 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6622 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2566 2989 3838 2566 Size: 423 849 5350 Fragments arranged by size: 5350 849 423 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2228 2264 2409 2476 2561 2735 2959 3872 Size: 989 36 145 67 85 174 224 913 Cuts at: 3872 5282 1239 Size: 1410 2579 Fragments arranged by size: 2579 1410 989 913 224 174 145 85 67 36 Bpu10I CC'TnA_GC Cuts at: 3813 3813 Size: 6622 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2294 2789 3119 4747 5612 5910 2294 Size: 495 330 1628 865 298 3006 Fragments arranged by size: 3006 1628 865 495 330 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2530 5285 882 Size: 617 1031 2755 2219 Fragments arranged by size: 2755 2219 1031 617 BsaAI yAC'GTr Cuts at: 493 1204 2098 4413 493 Size: 711 894 2315 2702 Fragments arranged by size: 2702 2315 894 711 BsaBI GATnn'nnATC Cuts at: 6616 6616 Size: 6622 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2298 3231 4429 4811 Size: 53 83 186 936 765 933 1198 382 Cuts at: 4811 5446 275 Size: 635 1451 Fragments arranged by size: 1451 1198 936 933 765 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3040 5055 5453 5727 5874 6285 3040 Size: 2015 398 274 147 411 3377 Fragments arranged by size: 3377 2015 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2382 2412 2915 2945 3389 Size: 30 165 30 979 30 503 30 444 Cuts at: 3389 3419 1178 Size: 30 4381 Fragments arranged by size: 4381 979 503 444 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2261 2264 2518 3869 1186 Size: 1075 3 254 1351 3939 Fragments arranged by size: 3939 1351 1075 254 3 BseYI C'CCAG_C Cuts at: 2026 2102 2153 3514 5825 6321 2026 Size: 76 51 1361 2311 496 2327 Fragments arranged by size: 2327 2311 1361 496 76 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2504 2720 3466 1163 Size: 573 768 216 746 4319 Fragments arranged by size: 4319 768 746 573 216 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4072 4833 5861 1540 Size: 275 186 2071 761 1028 2301 Fragments arranged by size: 2301 2071 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2184 2492 2552 3095 3675 4683 4768 5839 2184 Size: 308 60 543 580 1008 85 1071 2967 Fragments arranged by size: 2967 1071 1008 580 543 308 85 60 BsmI GAATG_Cn' Cuts at: 4133 4226 4133 Size: 93 6529 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2103 2165 2184 2492 2552 2576 2823 Size: 399 313 62 19 308 60 24 247 Cuts at: 2823 3060 3095 3480 3641 3655 3675 3708 3819 Size: 237 35 385 161 14 20 33 111 Cuts at: 3819 3941 4683 4768 5839 1391 Size: 122 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 385 313 308 247 237 161 122 111 85 62 60 35 33 24 20 19 14 BspEI T'CCGG_A Cuts at: 6285 6285 Size: 6622 BspHI T'CATG_A Cuts at: 4511 6241 4511 Size: 1730 4892 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2195 2742 2808 3104 3610 3717 3977 844 Size: 1351 547 66 296 506 107 260 3489 Fragments arranged by size: 3489 1351 547 506 296 260 107 66 BsrBI CCG'CTC Cuts at: 1394 1823 3331 3822 4509 1394 Size: 429 1508 491 687 3507 Fragments arranged by size: 3507 1508 687 491 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2868 5117 5291 65 Size: 1183 1620 2249 174 1396 Fragments arranged by size: 2249 1620 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5266 5453 1700 Size: 110 3456 187 2869 Fragments arranged by size: 3456 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6622 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6622 BssSI C'ACGA_G Cuts at: 4682 5694 4682 Size: 1012 5610 BstAPI GCAn_nnn'nTGC Cuts at: 794 2074 3896 794 Size: 1280 1822 3520 Fragments arranged by size: 3520 1822 1280 BstBI TT'CG_AA Cuts at: 5426 5426 Size: 6622 BstEII G'GTnAC_C Cuts at: 1184 2470 1184 Size: 1286 5336 BstXI CCAn_nnnn'nTGG Cuts at: 2387 2443 2569 2987 3226 3502 3514 2387 Size: 56 126 418 239 276 12 5495 Fragments arranged by size: 5495 418 276 239 126 56 12 BstYI r'GATC_y Cuts at: 1972 2124 2463 2913 3074 3952 4319 4704 4721 Size: 152 339 450 161 878 367 385 17 Cuts at: 4721 6173 6259 6271 6617 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 878 450 385 367 346 339 161 152 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2109 2891 2109 Size: 782 5840 BtgI C'CryG_G Cuts at: 513 1065 2059 2664 2776 3000 3219 3363 3606 Size: 552 994 605 112 224 219 144 243 Cuts at: 3606 513 Size: 3529 Fragments arranged by size: 3529 994 605 552 243 224 219 144 112 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5883 Fragments arranged by size: 5883 683 56 BtsI GCAGTG_nn' Cuts at: 2222 3890 4138 4932 4952 2222 Size: 1668 248 794 20 3892 Fragments arranged by size: 3892 1668 794 248 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6622 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6587 DraI TTT'AAA Cuts at: 3926 3926 Size: 6622 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5629 809 Size: 507 4313 1802 Fragments arranged by size: 4313 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2445 2782 3147 Size: 54 1425 50 275 18 615 337 365 Cuts at: 3147 3276 3366 3384 3504 3528 4069 4958 6587 Size: 129 90 18 120 24 541 889 1629 Cuts at: 6587 8 Size: 43 Fragments arranged by size: 1629 1425 889 615 541 365 337 275 129 120 90 54 50 43 24 18 18 EagI C'GGCC_G Cuts at: 1537 1812 4069 1537 Size: 275 2257 4090 Fragments arranged by size: 4090 2257 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2348 4552 1353 Size: 995 2204 3423 Fragments arranged by size: 3423 2204 995 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5209 5583 5729 387 Size: 1064 138 3620 374 146 1280 Fragments arranged by size: 3620 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2490 2550 2490 Size: 60 6562 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2228 2264 2409 2476 2561 2735 2959 Size: 393 596 36 145 67 85 174 224 Cuts at: 2959 3782 3872 4685 5282 6069 6337 1239 Size: 823 90 813 597 787 268 1524 Fragments arranged by size: 1524 823 813 787 597 596 393 268 224 174 145 90 85 67 36 EcoNI CCTnn'n_nnAGG Cuts at: 2246 2618 3134 2246 Size: 372 516 5734 Fragments arranged by size: 5734 516 372 EcoO109I rG'GnC_Cy Cuts at: 2031 2113 2133 2162 2203 3321 3651 3652 2031 Size: 82 20 29 41 1118 330 1 5001 Fragments arranged by size: 5001 1118 330 82 41 29 20 1 EcoRI G'AATT_C Cuts at: 3931 4309 3931 Size: 378 6244 FspI TGC'GCA Cuts at: 5128 5128 Size: 6622 HaeII r_GCGC'y Cuts at: 1678 2215 2301 3234 3414 3515 5769 1678 Size: 537 86 933 180 101 2254 2531 Fragments arranged by size: 2531 2254 933 537 180 101 86 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3389 3421 3682 3714 5263 Size: 32 135 32 1817 32 261 32 1549 Cuts at: 5263 5295 5337 5369 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1817 1549 261 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3966 4212 669 Size: 988 281 2028 246 3079 Fragments arranged by size: 3079 2028 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6622 HpaI GTT'AAC Cuts at: 4212 4212 Size: 6622 KasI G'GCGC_C Cuts at: 2297 3230 2297 Size: 933 5689 KpnI G_GTAC'C Cuts at: 3472 3947 3472 Size: 475 6147 MfeI C'AATT_G Cuts at: 4221 4221 Size: 6622 MluI A'CGCG_T Cuts at: 6498 6498 Size: 6622 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2460 2601 3369 3674 5736 5920 1305 Size: 1155 141 768 305 2062 184 2007 Fragments arranged by size: 2062 2007 1155 768 305 184 141 MscI TGG'CCA Cuts at: 10 64 1489 1832 3149 3386 3506 10 Size: 54 1425 343 1317 237 120 3126 Fragments arranged by size: 3126 1425 1317 343 237 120 54 MslI CAynn'nnrTG Cuts at: 518 1166 2567 2606 2951 3023 3224 3500 3605 Size: 648 1401 39 345 72 201 276 105 Cuts at: 3605 3906 4580 4939 5098 518 Size: 301 674 359 159 2042 Fragments arranged by size: 2042 1401 674 648 359 345 301 276 201 159 105 72 39 MspA1I CmG'CkG Cuts at: 1606 2383 2845 3540 3832 4038 4715 5863 6108 Size: 777 462 695 292 206 677 1148 245 Cuts at: 6108 1606 Size: 2120 Fragments arranged by size: 2120 1148 777 695 677 462 292 245 206 NarI GG'CG_CC Cuts at: 2298 3231 2298 Size: 933 5689 NcoI C'CATG_G Cuts at: 513 1065 2776 3000 3219 3606 513 Size: 552 1711 224 219 387 3529 Fragments arranged by size: 3529 1711 552 387 224 219 NdeI CA'TA_TG Cuts at: 387 387 Size: 6622 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6622 NotI GC'GGCC_GC Cuts at: 4069 4069 Size: 6622 NspI r_CATG'y Cuts at: 1132 1229 2072 2365 3980 5525 1132 Size: 97 843 293 1615 1545 2229 Fragments arranged by size: 2229 1615 1545 843 293 97 PasI CC'CwG_GG Cuts at: 1348 3393 3840 1348 Size: 2045 447 4130 Fragments arranged by size: 4130 2045 447 PciI A'CATG_T Cuts at: 5521 5521 Size: 6622 PflMI CCAn_nnn'nTGG Cuts at: 1108 2060 1108 Size: 952 5670 PfoI T'CCnGG_A Cuts at: 2205 3849 2205 Size: 1644 4978 PmeI GTTT'AAAC Cuts at: 3926 3926 Size: 6622 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4691 4723 6204 6236 4691 Size: 32 1481 32 5077 Fragments arranged by size: 5077 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2031 2113 2133 2203 2031 Size: 82 20 70 6450 Fragments arranged by size: 6450 82 70 20 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6622 PsiI TTA'TAA Cuts at: 4192 4192 Size: 6622 PspOMI G'GGCC_C Cuts at: 2161 3651 2161 Size: 1490 5132 PstI C_TGCA'G Cuts at: 830 3690 3974 830 Size: 2860 284 3478 Fragments arranged by size: 3478 2860 284 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6622 PvuII CAG'CTG Cuts at: 2383 2845 2383 Size: 462 6160 SacI G_AGCT'C Cuts at: 2492 2552 2492 Size: 60 6562 SalI G'TCGA_C Cuts at: 1936 3964 1936 Size: 2028 4594 SanDI GG'GwC_CC Cuts at: 2031 2031 Size: 6622 SbfI CC_TGCA'GG Cuts at: 3974 3974 Size: 6622 ScaI AGT'ACT Cuts at: 4870 6582 4870 Size: 1712 4910 SfcI C'TryA_G Cuts at: 826 1045 3686 3970 5105 5786 5977 826 Size: 219 2641 284 1135 681 191 1471 Fragments arranged by size: 2641 1471 1135 681 284 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3659 3659 Size: 6622 SfoI GGC'GCC Cuts at: 2299 3232 2299 Size: 933 5689 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6622 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6622 SmaI CCC'GGG Cuts at: 1872 2130 3703 3949 1872 Size: 258 1573 246 4545 Fragments arranged by size: 4545 1573 258 246 SmlI C'TyrA_G Cuts at: 820 1017 1943 2309 2804 3134 4726 5627 5889 Size: 197 926 366 495 330 1592 901 262 Cuts at: 5889 820 Size: 1553 Fragments arranged by size: 1592 1553 926 901 495 366 330 262 197 SnaBI TAC'GTA Cuts at: 493 4413 493 Size: 3920 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6622 SphI G_CATG'C Cuts at: 1132 3980 1132 Size: 2848 3774 SspI AAT'ATT Cuts at: 5 52 4546 5 Size: 47 4494 2081 Fragments arranged by size: 4494 2081 47 StuI AGG'CCT Cuts at: 1517 3811 1517 Size: 2294 4328 StyI C'CwwG_G Cuts at: 513 1065 2776 2986 3000 3150 3219 3507 3606 Size: 552 1711 210 14 150 69 288 99 Cuts at: 3606 4061 513 Size: 455 3074 Fragments arranged by size: 3074 1711 552 455 288 210 150 99 69 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2940 3284 4649 4666 4819 5004 5449 Size: 1255 1360 344 1365 17 153 185 445 Cuts at: 5449 325 Size: 1498 Fragments arranged by size: 1498 1365 1360 1255 445 344 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4868 6580 96 Size: 274 80 33 51 1443 2891 1712 138 Fragments arranged by size: 2891 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2040 2072 2382 2414 2915 2947 3066 3098 6582 Size: 32 310 32 501 32 119 32 3484 Cuts at: 6582 6614 2040 Size: 32 2048 Fragments arranged by size: 3484 2048 501 310 119 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6622 XbaI T'CTAG_A Cuts at: 3958 3958 Size: 6622 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2239 2605 3370 3847 2239 Size: 366 765 477 5014 Fragments arranged by size: 5014 765 477 366 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6622 XmaI C'CCGG_G Cuts at: 1870 2128 3701 3947 1870 Size: 258 1573 246 4545 Fragments arranged by size: 4545 1573 258 246 XmnI GAAnn'nnTTC Cuts at: 4751 4751 Size: 6622 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4430 276 Size: 53 83 186 936 2896 2468 Fragments arranged by size: 2896 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SrfI SwaI