################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BanI BbeI BbrPI BcgI BciVI BclI BcnI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsmI Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I CelII CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FbaI FokI FseI FspI HaeII Hin1I HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI MslI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI SspI SwaI Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccII 445 CGCG CG^CG AflII 1632 CTTAAG C^TTAAG AflIII 459 ACGTGT A^C[GA][CT]GT AspI 1458 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1417 CTCGGG C^[CT]CG[GA]G AvaII 720 GGACC G^G[AT]CC BamHI 744 GGATCC G^GATCC BanII 1205 GGGCTC G[GA]GC[CT]^C BbvCI 1624 GCTGAGG CCTCAGC(-5/-2) BfrI 1632 CTTAAG C^TTAAG BglII 702 AGATCT A^GATCT BsiYI 303 CCATGGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 303 CCATGGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsoBI 1417 CTCGGG C^[CT]CG[GA]G BspDI 1433 ATCGAT AT^CGAT BsrDI 799 GCAATG GCAATG(2/0) BsrFI 617 ACCGGC [GA]^CCGG[CT] BssSI 686 CTCGTG CACGAG(-5/-1) BstUI 445 CGCG CG^CG Cfr10I 617 ACCGGC [GA]^CCGG[CT] Cfr13I 720 GGACC G^G[ACGT]CC ClaI 1433 ATCGAT AT^CGAT EcoT22I 1394 ATGCAT ATGCA^T HaeIII 1629 GGCC GG^CC HapII 618 CCGG C^CGG HgaI 1621 GACGC GACGC(5/10) HindIII 1687 AAGCTT A^AGCTT HpaII 618 CCGG C^CGG MfeI 118 CAATTG C^AATTG MspI 618 CCGG C^CGG MunI 118 CAATTG C^AATTG MvnI 445 CGCG CG^CG NcoI 303 CCATGG C^CATGG NlaIV 744 GGATCC GG[ACGT]^[ACGT]CC NsiI 1394 ATGCAT ATGCA^T PflFI 1458 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC SalI 1066 GTCGAC G^TCGAC Sau96I 720 GGACC G^G[ACGT]CC SfcI 910 CTACAG C^T[GA][CT]AG SpeI 87 ACTAGT A^CTAGT StuI 1628 AGGCCT AGG^CCT Tsp45I 840 GTCAC ^GT[GC]AC Tth111I 1458 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1209 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 613 GTAGAC GT^[AC][GT]AC 1066 GTCGAC AlwI 745 GATCC GGATC(4/5) 744 GGATC AspHI 1363 GTGCTC G[AT]GC[AT]^C 1412 GTGCTC BsaI 1795 GAGACC GGTCTC(1/5) 923 GGTCTC BsgI 68 CTGCAC GTGCAG(16/14) 463 GTGCAG BsiHKAI 1363 GTGCTC G[AT]GC[AT]^C 1412 GTGCTC BsrI 1528 CCAGT ACTGG(1/-1) 1800 CCAGT BstYI 702 AGATCT [GA]^GATC[CT] 744 GGATCC CfoI 444 GCGC GCG^C 606 GCGC DsaI 303 CCATGG C^C[GA][CT]GG 1562 CCGTGG EcoT14I 303 CCATGG C^C[AT][AT]GG 740 CCTTGG FauI 1615 CCCGC CCCGC(4/6) 1203 GCGGG HhaI 444 GCGC GCG^C 606 GCGC HinP1I 444 GCGC G^CGC 606 GCGC HincII 1066 GTCGAC GT[CT]^[GA]AC 1402 GTCAAC HindII 1066 GTCGAC GT[CT]^[GA]AC 1402 GTCAAC MaeIII 840 GTCAC ^GT[ACGT]AC 891 GTTAC MflI 702 AGATCT [GA]^GATC[CT] 744 GGATCC NspI 473 GCATGT [GA]CATG^[CT] 1396 GCATGT PvuII 385 CAGCTG CAG^CTG 1573 CAGCTG SapI 52 GAAGAGC GCTCTTC(1/4) 493 GCTCTTC SmlI 202 CTCAAG C^T[CT][GA]AG 1632 CTTAAG StyI 303 CCATGG C^C[AT][AT]GG 740 CCTTGG TspRI 1410 CAGTG CA[GC]TG[ACGT][ACGT]^ 1801 CAGTG XhoII 702 AGATCT [GA]^GATC[CT] 744 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsmAI Bsp1286I BssKI BstNI Cac8I DdeI DpnII EarI EcoRII Fnu4HI HinfI HphI ItaI Ksp632I MaeI MaeII MboI MboII MnlI MseI MspA1I MvaI MwoI NdeII NlaIII PleI RsaI Sau3AI ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp509I TthHB8I