(Circular) (Six-base) MAPSORT of: pFN21AE9691.seq Check: 6235 from: 1 to: 6520 pFN21AE9691 6520 bp With 182 enzymes: SgfI * December 8, 2014 13:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4330 278 Size: 53 83 186 936 2794 2468 Fragments arranged by size: 2794 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2619 3072 3863 1937 Size: 682 453 791 4594 Fragments arranged by size: 4594 791 682 453 Acc65I G'GTAC_C Cuts at: 3841 3841 Size: 6520 AclI AA'CG_TT Cuts at: 1563 4647 5020 1563 Size: 3084 373 3063 Fragments arranged by size: 3084 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2073 2414 2469 2696 3051 3101 3345 3602 Size: 441 341 55 227 355 50 244 257 Cuts at: 3602 4583 5967 6235 1632 Size: 981 1384 268 1917 Fragments arranged by size: 1917 1384 981 441 355 341 268 257 244 227 55 50 AflII C'TTAA_G Cuts at: 820 1017 3637 820 Size: 197 2620 3703 Fragments arranged by size: 3703 2620 197 AflIII A'CryG_T Cuts at: 1203 2464 5419 6396 1203 Size: 1261 2955 977 1327 Fragments arranged by size: 2955 1327 1261 977 AgeI A'CCGG_T Cuts at: 5351 5351 Size: 6520 AhdI GACnn_n'nnGTC Cuts at: 5249 5249 Size: 6520 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6520 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6520 AlwNI CAG_nnn'CTG Cuts at: 4263 5835 4263 Size: 1572 4948 ApaLI G'TGCA_C Cuts at: 4577 5733 4577 Size: 1156 5364 ApoI r'AATT_y Cuts at: 1493 2939 3029 3037 3829 4055 4207 6316 1493 Size: 1446 90 8 792 226 152 2109 1697 Fragments arranged by size: 2109 1697 1446 792 226 152 90 8 AseI AT'TA_AT Cuts at: 160 1032 5074 160 Size: 872 4042 1606 Fragments arranged by size: 4042 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6520 AvaI C'yCGr_G Cuts at: 1870 1943 3422 3845 1870 Size: 73 1479 423 4545 Fragments arranged by size: 4545 1479 423 73 BamHI G'GATC_C Cuts at: 2749 3850 4217 2749 Size: 1101 367 5052 Fragments arranged by size: 5052 1101 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3841 5296 618 Size: 325 217 627 2054 1455 1842 Fragments arranged by size: 2054 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3214 3839 1391 Size: 1823 625 4072 Fragments arranged by size: 4072 1823 625 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2475 2540 3774 928 Size: 971 576 65 1234 3674 Fragments arranged by size: 3674 1234 971 576 65 BbvCI CC'TCA_GC Cuts at: 3630 3630 Size: 6520 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4711 4745 1903 Size: 34 2774 34 3678 Fragments arranged by size: 3678 2774 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4412 5622 4412 Size: 1210 5310 BclI T'GATC_A Cuts at: 1548 1548 Size: 6520 BfrBI ATG'CAT Cuts at: 3401 3401 Size: 6520 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5131 136 Size: 107 122 71 631 605 337 3122 1525 Fragments arranged by size: 3122 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2707 6515 2707 Size: 3808 2712 BlpI GC'TnA_GC Cuts at: 3937 3937 Size: 6520 Bme1580I G_kGCm'C Cuts at: 1790 3812 4581 5737 1790 Size: 2022 769 1156 2573 Fragments arranged by size: 2573 2022 1156 769 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6520 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3799 4251 5209 Size: 416 53 83 399 24 2382 452 958 Cuts at: 5209 442 Size: 1753 Fragments arranged by size: 2382 1753 958 452 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6520 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2297 2720 3044 3239 3780 5180 1239 Size: 1058 423 324 195 541 1400 2579 Fragments arranged by size: 2579 1400 1058 541 423 324 195 Bpu10I CC'TnA_GC Cuts at: 3630 3630 Size: 6520 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2192 4645 5510 5808 2192 Size: 2453 865 298 2904 Fragments arranged by size: 2904 2453 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2934 3794 5183 882 Size: 617 1435 860 1389 2219 Fragments arranged by size: 2219 1435 1389 860 617 BsaAI yAC'GTr Cuts at: 493 1204 4311 493 Size: 711 3107 2702 Fragments arranged by size: 3107 2702 711 BsaBI GATnn'nnATC Cuts at: 6514 6514 Size: 6520 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4327 4709 5344 275 Size: 53 83 186 936 2794 382 635 1451 Fragments arranged by size: 2794 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4953 5351 5625 5772 6183 4953 Size: 398 274 147 411 5290 Fragments arranged by size: 5290 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3452 3482 1178 Size: 30 165 30 2049 30 4216 Fragments arranged by size: 4216 2049 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2414 2657 3473 3540 1186 Size: 1228 243 816 67 4166 Fragments arranged by size: 4166 1228 816 243 67 BseYI C'CCAG_C Cuts at: 5723 6219 5723 Size: 496 6024 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2058 2489 1163 Size: 573 322 431 5194 Fragments arranged by size: 5194 573 431 322 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3970 4731 5759 1540 Size: 275 186 1969 761 1028 2301 Fragments arranged by size: 2301 1969 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3372 3421 4581 4666 5737 3372 Size: 49 1160 85 1071 4155 Fragments arranged by size: 4155 1160 1071 85 49 BsmI GAATG_Cn' Cuts at: 4031 4124 4031 Size: 93 6427 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3214 3372 3421 3812 3839 4581 4666 Size: 399 1424 158 49 391 27 742 85 Cuts at: 4666 5737 1391 Size: 1071 2174 Fragments arranged by size: 2174 1424 1071 742 399 391 158 85 49 27 BspEI T'CCGG_A Cuts at: 6183 6183 Size: 6520 BspHI T'CATG_A Cuts at: 4409 6139 4409 Size: 1730 4790 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3875 844 Size: 3031 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4407 1394 Size: 429 2584 3507 Fragments arranged by size: 3507 2584 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2811 5015 5189 65 Size: 1183 1563 2204 174 1396 Fragments arranged by size: 2204 1563 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2622 5164 5351 1700 Size: 110 812 2542 187 2869 Fragments arranged by size: 2869 2542 812 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6520 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6520 BssSI C'ACGA_G Cuts at: 2691 4580 5592 2691 Size: 1889 1012 3619 Fragments arranged by size: 3619 1889 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6520 BstBI TT'CG_AA Cuts at: 5324 5324 Size: 6520 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6520 BstYI r'GATC_y Cuts at: 1972 2707 2749 3850 4217 4602 4619 6071 6157 Size: 735 42 1101 367 385 17 1452 86 Cuts at: 6157 6169 6515 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1101 735 385 367 346 86 42 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2308 3567 513 Size: 552 939 304 1259 3466 Fragments arranged by size: 3466 1259 939 552 304 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5781 Fragments arranged by size: 5781 683 56 BtsI GCAGTG_nn' Cuts at: 4036 4830 4850 4036 Size: 794 20 5706 Fragments arranged by size: 5706 794 20 ClaI AT'CG_AT Cuts at: 1554 3439 1554 Size: 1885 4635 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6485 DraI TTT'AAA Cuts at: 3824 3824 Size: 6520 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5527 809 Size: 507 4211 1802 Fragments arranged by size: 4211 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3967 4856 6485 Size: 54 1425 50 275 18 2137 889 1629 Cuts at: 6485 8 Size: 43 Fragments arranged by size: 2137 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3967 1537 Size: 275 2155 4090 Fragments arranged by size: 4090 2155 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2052 2505 3321 4450 1353 Size: 699 453 816 1129 3423 Fragments arranged by size: 3423 1129 816 699 453 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5107 5481 5627 387 Size: 1064 138 3518 374 146 1280 Fragments arranged by size: 3518 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2073 2297 2414 2469 2696 2720 3044 Size: 393 441 224 117 55 227 24 324 Cuts at: 3044 3051 3101 3239 3345 3602 3780 4583 5180 Size: 7 50 138 106 257 178 803 597 Cuts at: 5180 5967 6235 1239 Size: 787 268 1524 Fragments arranged by size: 1524 803 787 597 441 393 324 268 257 227 224 178 138 117 106 55 50 24 7 EcoRI G'AATT_C Cuts at: 3829 4207 3829 Size: 378 6142 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2393 2499 2393 Size: 106 6414 FspI TGC'GCA Cuts at: 5026 5026 Size: 6520 HaeII r_GCGC'y Cuts at: 1678 5667 1678 Size: 3989 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2025 2057 2482 2514 3452 Size: 32 135 32 453 32 425 32 938 Cuts at: 3452 3484 5161 5193 5235 5267 1373 Size: 32 1677 32 42 32 2626 Fragments arranged by size: 2626 1677 938 453 425 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3073 3409 3864 4110 669 Size: 988 281 1135 336 455 246 3079 Fragments arranged by size: 3079 1135 988 455 336 281 246 HindIII A'AGCT_T Cuts at: 748 3692 748 Size: 2944 3576 HpaI GTT'AAC Cuts at: 4110 4110 Size: 6520 KpnI G_GTAC'C Cuts at: 3845 3845 Size: 6520 MfeI C'AATT_G Cuts at: 2123 4119 2123 Size: 1996 4524 MluI A'CGCG_T Cuts at: 6396 6396 Size: 6520 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2703 2952 5634 5818 1305 Size: 1398 249 2682 184 2007 Fragments arranged by size: 2682 2007 1398 249 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4698 Fragments arranged by size: 4698 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4478 4837 4996 518 Size: 648 3312 359 159 2042 Fragments arranged by size: 3312 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2392 3208 3580 3623 3936 4613 5761 6006 Size: 786 816 372 43 313 677 1148 245 Cuts at: 6006 1606 Size: 2120 Fragments arranged by size: 2120 1148 816 786 677 372 313 245 43 NcoI C'CATG_G Cuts at: 513 1065 2004 2308 513 Size: 552 939 304 4725 Fragments arranged by size: 4725 939 552 304 NdeI CA'TA_TG Cuts at: 387 387 Size: 6520 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6520 NotI GC'GGCC_GC Cuts at: 3967 3967 Size: 6520 NsiI A_TGCA'T Cuts at: 3403 3403 Size: 6520 NspI r_CATG'y Cuts at: 1132 1229 2482 3405 3878 5423 1132 Size: 97 1253 923 473 1545 2229 Fragments arranged by size: 2229 1545 1253 923 473 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6520 PciI A'CATG_T Cuts at: 5419 5419 Size: 6520 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6520 PmeI GTTT'AAAC Cuts at: 3824 3824 Size: 6520 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4589 4621 6102 6134 4589 Size: 32 1481 32 4975 Fragments arranged by size: 4975 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6520 PsiI TTA'TAA Cuts at: 4090 4090 Size: 6520 PstI C_TGCA'G Cuts at: 830 3872 830 Size: 3042 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6520 PvuII CAG'CTG Cuts at: 2392 3580 2392 Size: 1188 5332 SalI G'TCGA_C Cuts at: 1936 3071 3862 1936 Size: 1135 791 4594 Fragments arranged by size: 4594 1135 791 SapI GCTCTTCn'nnn_ Cuts at: 2052 2505 2052 Size: 453 6067 SbfI CC_TGCA'GG Cuts at: 3872 3872 Size: 6520 ScaI AGT'ACT Cuts at: 4768 6480 4768 Size: 1712 4808 SfcI C'TryA_G Cuts at: 826 1045 2915 3868 5003 5684 5875 826 Size: 219 1870 953 1135 681 191 1471 Fragments arranged by size: 1870 1471 1135 953 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6520 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6520 SmaI CCC'GGG Cuts at: 1872 3847 1872 Size: 1975 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2207 3637 4624 5525 5787 820 Size: 197 926 264 1430 987 901 262 1553 Fragments arranged by size: 1553 1430 987 926 901 264 262 197 SnaBI TAC'GTA Cuts at: 493 4311 493 Size: 3818 2702 SpeI A'CTAG_T Cuts at: 152 2092 152 Size: 1940 4580 SphI G_CATG'C Cuts at: 1132 3878 1132 Size: 2746 3774 SspI AAT'ATT Cuts at: 5 52 4444 5 Size: 47 4392 2081 Fragments arranged by size: 4392 2081 47 StuI AGG'CCT Cuts at: 1517 3635 1517 Size: 2118 4402 StyI C'CwwG_G Cuts at: 513 1065 2004 2308 2745 3959 513 Size: 552 939 304 437 1214 3074 Fragments arranged by size: 3074 1214 939 552 437 304 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4547 4564 4717 4902 5347 325 Size: 1255 2967 17 153 185 445 1498 Fragments arranged by size: 2967 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3346 4766 6478 Size: 274 80 33 51 1443 1369 1420 1712 Cuts at: 6478 96 Size: 138 Fragments arranged by size: 1712 1443 1420 1369 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2304 2336 6480 6512 2304 Size: 32 4144 32 2312 Fragments arranged by size: 4144 2312 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3466 1316 Size: 2150 4370 XbaI T'CTAG_A Cuts at: 3214 3856 3214 Size: 642 5878 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6520 XmaI C'CCGG_G Cuts at: 1870 3845 1870 Size: 1975 4545 XmnI GAAnn'nnTTC Cuts at: 4649 4649 Size: 6520 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4328 276 Size: 53 83 186 936 2794 2468 Fragments arranged by size: 2794 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI