Restriction map for
pFN21AE9691
(Linear) (Six-base) MAPSORT of: KIEE9691.seq Check: 4450 from: 1 to: 1815
KIEE9691.orf
With 182 enzymes: SgfI *
December 5, 2014 13:30 ..
AccI GT'mk_AC
Cuts at: 0 614 1067 1815
Size: 614 453 748
Fragments arranged by size:
748 614 453
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 409 464 691 1046 1096 1340 1597
Size: 68 341 55 227 355 50 244 257
Cuts at: 1597 1815
Size: 218
Fragments arranged by size:
355 341 257 244 227 218 68 55
50
AflII C'TTAA_G
Cuts at: 0 1632 1815
Size: 1632 183
AflIII A'CryG_T
Cuts at: 0 459 1815
Size: 459 1356
ApoI r'AATT_y
Cuts at: 0 934 1024 1032 1815
Size: 934 90 8 783
Fragments arranged by size:
934 783 90 8
AvaI C'yCGr_G
Cuts at: 0 1417 1815
Size: 1417 398
BamHI G'GATC_C
Cuts at: 0 744 1815
Size: 744 1071
BanII G_rGCy'C
Cuts at: 0 1209 1815
Size: 1209 606
BbsI GAAGACnn'nnnn_
Cuts at: 0 470 535 1769 1815
Size: 470 65 1234 46
Fragments arranged by size:
1234 470 65 46
BbvCI CC'TCA_GC
Cuts at: 0 1625 1815
Size: 1625 190
BfrBI ATG'CAT
Cuts at: 0 1396 1815
Size: 1396 419
BglII A'GATC_T
Cuts at: 0 702 1815
Size: 702 1113
Bme1580I G_kGCm'C
Cuts at: 0 1807 1815
Size: 1807 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 1794 1815
Size: 1794 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 715 1039 1234 1775 1815
Size: 292 423 324 195 541 40
Fragments arranged by size:
541 423 324 292 195 40
Bpu10I CC'TnA_GC
Cuts at: 0 1625 1815
Size: 1625 190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 1815
Size: 187 1628
BsaI GGTCTCn'nnnn_
Cuts at: 0 929 1789 1815
Size: 929 860 26
Fragments arranged by size:
929 860 26
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1447 1477 1815
Size: 1447 30 338
Fragments arranged by size:
1447 338 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 409 652 1468 1535 1815
Size: 409 243 816 67 280
Fragments arranged by size:
816 409 280 243 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 484 1815
Size: 53 431 1331
Fragments arranged by size:
1331 431 53
BsiHKAI G_wGCw'C
Cuts at: 0 1367 1416 1815
Size: 1367 49 399
Fragments arranged by size:
1367 399 49
Bsp1286I G_dGCh'C
Cuts at: 0 1209 1367 1416 1807 1815
Size: 1209 158 49 391 8
Fragments arranged by size:
1209 391 158 49 8
BsrDI GCAATG_nn'
Cuts at: 0 806 1815
Size: 806 1009
BsrFI r'CCGG_y
Cuts at: 0 617 1815
Size: 617 1198
BssSI C'ACGA_G
Cuts at: 0 686 1815
Size: 686 1129
BstYI r'GATC_y
Cuts at: 0 702 744 1815
Size: 702 42 1071
Fragments arranged by size:
1071 702 42
BtgI C'CryG_G
Cuts at: 0 303 1562 1815
Size: 303 1259 253
Fragments arranged by size:
1259 303 253
ClaI AT'CG_AT
Cuts at: 0 1434 1815
Size: 1434 381
EarI CTCTTCn'nnn_
Cuts at: 0 47 500 1316 1815
Size: 47 453 816 499
Fragments arranged by size:
816 499 453 47
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 292 409 464 691 715 1039 1046
Size: 68 224 117 55 227 24 324 7
Cuts at: 1046 1096 1234 1340 1597 1775 1815
Size: 50 138 106 257 178 40
Fragments arranged by size:
324 257 227 224 178 138 117 106
68 55 50 40 24 7
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 388 494 1815
Size: 388 106 1321
Fragments arranged by size:
1321 388 106
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 20 52 477 509 1447 1479 1815
Size: 20 32 425 32 938 32 336
Fragments arranged by size:
938 425 336 32 32 32 20
HincII GTy'rAC
Cuts at: 0 1068 1404 1815
Size: 1068 336 411
Fragments arranged by size:
1068 411 336
HindIII A'AGCT_T
Cuts at: 0 1687 1815
Size: 1687 128
MfeI C'AATT_G
Cuts at: 0 118 1815
Size: 118 1697
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 947 1815
Size: 698 249 868
Fragments arranged by size:
868 698 249
MspA1I CmG'CkG
Cuts at: 0 387 1203 1575 1618 1815
Size: 387 816 372 43 197
Fragments arranged by size:
816 387 372 197 43
NcoI C'CATG_G
Cuts at: 0 303 1815
Size: 303 1512
NsiI A_TGCA'T
Cuts at: 0 1398 1815
Size: 1398 417
NspI r_CATG'y
Cuts at: 0 477 1400 1815
Size: 477 923 415
Fragments arranged by size:
923 477 415
PvuII CAG'CTG
Cuts at: 0 387 1575 1815
Size: 387 1188 240
Fragments arranged by size:
1188 387 240
SalI G'TCGA_C
Cuts at: 0 1066 1815
Size: 1066 749
SapI GCTCTTCn'nnn_
Cuts at: 0 47 500 1815
Size: 47 453 1315
Fragments arranged by size:
1315 453 47
SfcI C'TryA_G
Cuts at: 0 910 1815
Size: 910 905
SmlI C'TyrA_G
Cuts at: 0 202 1632 1815
Size: 202 1430 183
Fragments arranged by size:
1430 202 183
SpeI A'CTAG_T
Cuts at: 0 87 1815
Size: 87 1728
StuI AGG'CCT
Cuts at: 0 1630 1815
Size: 1630 185
StyI C'CwwG_G
Cuts at: 0 303 740 1815
Size: 303 437 1075
Fragments arranged by size:
1075 437 303
TatI w'GTAC_w
Cuts at: 0 1341 1815
Size: 1341 474
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 299 331 1815
Size: 299 32 1484
Fragments arranged by size:
1484 299 32
Tth111I GACn'n_nGTC
Cuts at: 0 1461 1815
Size: 1461 354
XbaI T'CTAG_A
Cuts at: 0 1209 1815
Size: 1209 606
Enzymes that do cut:
AccI AcuI AflII AflIII ApoI AvaI BamHI BanII
BbsI BbvCI BfrBI BglII Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaXI BseRI BsgI BsiHKAI Bsp1286I BsrDI
BsrFI BssSI BstYI BtgI ClaI EarI Eco57MI FalI
Hin4I HincII HindIII MfeI MmeI MspA1I NcoI NsiI
NspI PvuII SalI SapI SfcI SmlI SpeI StuI
StyI TatI TstI Tth111I XbaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BanI BbeI BcgI BciVI BclI BglI
BlpI BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI
FspI FspAI HaeII HpaI KasI KpnI MluI MscI
MslI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SspI
SwaI TaqII XcmI XhoI XmaI XmnI ZraI