################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AfaI AflIII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BalI BbrPI BbvCI BcgI BclI BcnI BglI BlnI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoRV EcoT22I FauI FbaI FseI FspI HapII HpaII KpnI KspI MamI MluI MluNI MroI MscI MspI MvnI NaeI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Alw44I 50 GTGCAC G^TGCAC AlwNI 319 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 10 CCTAAGG CC^T[ACGT]AGG ApaLI 50 GTGCAC G^TGCAC AvaII 100 GGACC G^G[AT]CC BamHI 314 GGATCC G^GATCC BbeI 15 GGCGCC GGCGC^C BciVI 106 GTATCC GTATCC(6/5) BlpI 559 GCTAAGC GC^T[ACGT]AGC Bpu1102I 559 GCTAAGC GC^T[ACGT]AGC BsmBI 1741 CGTCTC CGTCTC(1/5) BspHI 2079 TCATGA T^CATGA BspMI 588 GCAGGT ACCTGC(4/8) BstAPI 394 GCAAATAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 10 CCTAAGG CC^T[ACGT]AGG CelII 559 GCTAAGC GC^T[ACGT]AGC CfoI 16 GCGC GCG^C Cfr13I 100 GGACC G^G[ACGT]CC DraI 130 TTTAAA TTT^AAA EaeI 1454 CGGCCA [CT]^GGCC[GA] Eco81I 10 CCTAAGG CC^T[ACGT]AGG EcoRI 494 GAATTC G^AATTC EcoT14I 1457 CCAAGG C^C[AT][AT]GG HaeII 15 GGCGCC [GA]GCGC^[CT] HgaI 1740 GCGTC GACGC(5/10) HhaI 16 GCGC GCG^C HinP1I 16 GCGC G^CGC HpaI 1395 GTTAAC GTT^AAC HphI 593 TCACC GGTGA(8/7) KasI 15 GGCGCC G^GCGCC MaeII 294 ACGT A^CGT MfeI 1204 CAATTG C^AATTG MslI 573 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 1204 CAATTG C^AATTG NarI 15 GGCGCC GG^CGCC NdeI 1874 CATATG CA^TATG NspI 1920 GCATGT [GA]CATG^[CT] PflMI 318 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 2079 TCATGA T^CATGA Sau96I 100 GGACC G^G[ACGT]CC SfoI 15 GGCGCC GGC^GCC SspI 835 AATATT AAT^ATT StyI 1457 CCAAGG C^C[AT][AT]GG Tsp45I 592 GTCAC ^GT[GC]AC Van91I 318 CCAGGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 15 GGCGCC G[GA]^CG[CT]C 1739 GGCGTC AflII 814 CTTAAG C^TTAAG 1420 CTTAAG Asp700I 24 GAAACGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1000 GAAAAGCTTC AspI 907 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1551 GACCAAGTC BanI 15 GGCGCC G^G[CT][GA]CC 298 GGTGCC BanII 1509 GAGCTC G[GA]GC[CT]^C 2144 GAGCTC BfrI 814 CTTAAG C^TTAAG 1420 CTTAAG BsaHI 15 GGCGCC G[GA]^CG[CT]C 1739 GGCGTC BseRI 1744 CTCCTC GAGGAG(10/8) 829 GAGGAG BsgI 365 CTGCAC GTGCAG(16/14) 1838 GTGCAG BstEII 475 GGTAACC G^GT[ACGT]ACC 591 GGTCACC BstPI 475 GGTAACC G^GT[ACGT]ACC 591 GGTCACC Cac8I 186 GCCTGC GC[ACGT]^[ACGT]GC 1986 GCTTGC EarI 610 GAAGAG CTCTTC(1/4) 1210 GAAGAG EcoO65I 475 GGTAACC G^GT[ACGT]ACC 591 GGTCACC HaeIII 1311 GGCC GG^CC 1455 GGCC Hin1I 15 GGCGCC G[GA]^CG[CT]C 1739 GGCGTC HindIII 501 AAGCTT A^AGCTT 1003 AAGCTT Ksp632I 610 GAAGAG CTCTTC(1/4) 1210 GAAGAG PflFI 907 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1551 GACCAAGTC PstI 586 CTGCAG CTGCA^G 1546 CTGCAG PvuII 883 CAGCTG CAG^CTG 1663 CAGCTG SacI 1509 GAGCTC GAGCT^C 2144 GAGCTC Tth111I 907 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 1551 GACCAAGTC XbaI 1225 TCTAGA T^CTAGA 1539 TCTAGA XmnI 24 GAAACGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1000 GAAAAGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AspHI BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI DdeI DpnII EcoRII Fnu4HI FokI HincII HindII HinfI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII